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UniProtKB - P45450 (MURF_SYNY3)
Protein
UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase
Gene
murF
Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Functioni
Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein.
UniRule annotationCatalytic activityi
- ATP + D-alanyl-D-alanine + UDP-N-acetyl-α-D-muramoyl-L-alanyl-γ-D-glutamyl-meso-2,6-diaminoheptanedioate = ADP + H+ + phosphate + UDP-N-acetyl-α-D-muramoyl-L-alanyl-γ-D-glutamyl-meso-2,6-diaminopimeloyl-D-alanyl-D-alanineUniRule annotationEC:6.3.2.10UniRule annotation
: peptidoglycan biosynthesis Pathwayi
This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotationView all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 116 – 122 | ATPUniRule annotation | 7 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-UniRule
- UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity Source: UniProtKB-EC
- UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase activity Source: UniProtKB-UniRule
GO - Biological processi
- cell cycle Source: UniProtKB-KW
- cell division Source: UniProtKB-KW
- cell wall organization Source: UniProtKB-KW
- peptidoglycan biosynthetic process Source: UniProtKB-UniRule
- regulation of cell shape Source: UniProtKB-KW
Keywordsi
Molecular function | Ligase |
Biological process | Cell cycle, Cell division, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
UniPathwayi | UPA00219 |
Names & Taxonomyi
Protein namesi | Recommended name: UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligaseUniRule annotation (EC:6.3.2.10UniRule annotation)Alternative name(s): D-alanyl-D-alanine-adding enzymeUniRule annotation UDP-MurNAc-pentapeptide synthetase |
Gene namesi | Name:murFUniRule annotation Ordered Locus Names:slr1351 |
Organismi | Synechocystis sp. (strain PCC 6803 / Kazusa) |
Taxonomic identifieri | 1111708 [NCBI] |
Taxonomic lineagei | Bacteria › Cyanobacteria › Synechococcales › Merismopediaceae › Synechocystis › unclassified Synechocystis › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm UniRule annotation
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000101706 | 1 – 454 | UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligaseAdd BLAST | 454 |
Proteomic databases
PaxDbi | P45450 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0770, Bacteria |
InParanoidi | P45450 |
OMAi | VEMGANH |
PhylomeDBi | P45450 |
Family and domain databases
Gene3Di | 3.40.1190.10, 1 hit 3.90.190.20, 1 hit |
HAMAPi | MF_02019, MurF, 1 hit |
InterProi | View protein in InterPro IPR036565, Mur-like_cat_sf IPR004101, Mur_ligase_C IPR036615, Mur_ligase_C_dom_sf IPR013221, Mur_ligase_cen IPR000713, Mur_ligase_N IPR035911, MurE/MurF_N IPR005863, UDP-N-AcMur_synth |
Pfami | View protein in Pfam PF01225, Mur_ligase, 1 hit PF02875, Mur_ligase_C, 1 hit PF08245, Mur_ligase_M, 1 hit |
SUPFAMi | SSF53244, SSF53244, 1 hit SSF53623, SSF53623, 1 hit SSF63418, SSF63418, 1 hit |
TIGRFAMsi | TIGR01143, murF, 1 hit |
i Sequence
Sequence statusi: Complete.
P45450-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNSKLSLFDI RTFLESCLLS VTNLSEDIRI NNICTDTRSL VSGDLFLALR
60 70 80 90 100
GESFDGHSFI PQALTAGAIA VVTDRPVEGL GETVAQFLVE DTLVAYQHIA
110 120 130 140 150
AGWRQRFTIP IIGVTGSVGK TTTKELIAAV LSQFGNVHKT RANYNNEIGV
160 170 180 190 200
PKTLLELSPD HDFAIVEMAM RGRGQIALLA DIAKPTIGLI TNVGTAHIGL
210 220 230 240 250
LGSELAIAEA KCELLAHQPP ESTAILNRDN ALLMETAQRF WQGKTITYGL
260 270 280 290 300
EGGDVHGTVD GENLILDGVS LPLPLAGVHN ASNYLAAIAL AQCLGLDWQQ
310 320 330 340 350
LQSGLTVELP KGRARRYQWG QDVVLLDETY NDGLESMLAS LDLLANTPGK
360 370 380 390 400
RRLAVLGAMK ELGDYGPTFH QRVGAKVKAL GLDGLFLLAN DPNTDAIAAG
410 420 430 440 450
ANGVETQSFS DGPSLVAALK TTLQPGDRLL FKASNSVGLG AVVSQLLAEN
PTSV
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 290 | L → V in BAA18171 (PubMed:8905231).Curated | 1 | |
Sequence conflicti | 332 | D → A in BAA18171 (PubMed:8905231).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X62437 Genomic DNA Translation: CAA44303.1 BA000022 Genomic DNA Translation: BAA18171.1 |
PIRi | S75610 |
Genome annotation databases
EnsemblBacteriai | BAA18171; BAA18171; BAA18171 |
KEGGi | syn:slr1351 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X62437 Genomic DNA Translation: CAA44303.1 BA000022 Genomic DNA Translation: BAA18171.1 |
PIRi | S75610 |
3D structure databases
AlphaFoldDBi | P45450 |
SMRi | P45450 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | P45450, 1 interactor |
STRINGi | 1148.1653256 |
Proteomic databases
PaxDbi | P45450 |
Genome annotation databases
EnsemblBacteriai | BAA18171; BAA18171; BAA18171 |
KEGGi | syn:slr1351 |
Phylogenomic databases
eggNOGi | COG0770, Bacteria |
InParanoidi | P45450 |
OMAi | VEMGANH |
PhylomeDBi | P45450 |
Enzyme and pathway databases
UniPathwayi | UPA00219 |
Family and domain databases
Gene3Di | 3.40.1190.10, 1 hit 3.90.190.20, 1 hit |
HAMAPi | MF_02019, MurF, 1 hit |
InterProi | View protein in InterPro IPR036565, Mur-like_cat_sf IPR004101, Mur_ligase_C IPR036615, Mur_ligase_C_dom_sf IPR013221, Mur_ligase_cen IPR000713, Mur_ligase_N IPR035911, MurE/MurF_N IPR005863, UDP-N-AcMur_synth |
Pfami | View protein in Pfam PF01225, Mur_ligase, 1 hit PF02875, Mur_ligase_C, 1 hit PF08245, Mur_ligase_M, 1 hit |
SUPFAMi | SSF53244, SSF53244, 1 hit SSF53623, SSF53623, 1 hit SSF63418, SSF63418, 1 hit |
TIGRFAMsi | TIGR01143, murF, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MURF_SYNY3 | |
Accessioni | P45450Primary (citable) accession number: P45450 Secondary accession number(s): P74091 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | November 1, 1995 | |
Last modified: | May 25, 2022 | |
This is version 134 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families