UniProtKB - P45448 (NR5A2_MOUSE)
Nuclear receptor subfamily 5 group A member 2
Nr5a2
Functioni
Nuclear receptor that acts as a key metabolic sensor by regulating the expression of genes involved in bile acid synthesis, cholesterol homeostasis and triglyceride synthesis. Together with the oxysterol receptors NR1H3/LXR-alpha and NR1H2/LXR-beta, acts as an essential transcriptional regulator of lipid metabolism. Plays an anti-inflammatory role during the hepatic acute phase response by acting as a corepressor: inhibits the hepatic acute phase response by preventing dissociation of the N-Cor corepressor complex. Key regulator of cholesterol 7-alpha-hydroxylase gene (CYP7A) expression in liver. May also contribute to the regulation of pancreas-specific genes and play important roles in embryonic development (By similarity).
Activates the transcription of CYP2C38 (PubMed:30555544).
By similarity1 PublicationSites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 535 | Phosphatidylglycerol phosphateBy similarity | 1 | |
Binding sitei | 539 | Phosphatidylglycerol phosphateBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 104 – 175 | Nuclear receptorPROSITE-ProRule annotationAdd BLAST | 72 | |
Zinc fingeri | 107 – 127 | NR C4-typePROSITE-ProRule annotationAdd BLAST | 21 | |
Zinc fingeri | 143 – 167 | NR C4-typePROSITE-ProRule annotationAdd BLAST | 25 |
GO - Molecular functioni
- chromatin binding Source: BHF-UCL
- DNA binding Source: MGI
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: NTNU_SB
- DNA-binding transcription factor activity Source: UniProtKB
- double-stranded DNA binding Source: MGI
- nuclear receptor activity Source: MGI
- phospholipid binding Source: MGI
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: NTNU_SB
- sequence-specific DNA binding Source: MGI
- sequence-specific double-stranded DNA binding Source: MGI
- transcription cis-regulatory region binding Source: MGI
- transcription coregulator binding Source: Ensembl
- zinc ion binding Source: InterPro
GO - Biological processi
- bile acid metabolic process Source: MGI
- calcineurin-mediated signaling Source: MGI
- cellular response to leukemia inhibitory factor Source: MGI
- cholesterol homeostasis Source: MGI
- epithelial cell differentiation Source: MGI
- hormone-mediated signaling pathway Source: GO_Central
- pancreas morphogenesis Source: MGI
- positive regulation of transcription, DNA-templated Source: UniProtKB
- positive regulation of transcription by RNA polymerase II Source: NTNU_SB
- positive regulation of viral genome replication Source: MGI
- regulation of cell population proliferation Source: MGI
- regulation of transcription, DNA-templated Source: UniProtKB
- regulation of transcription by RNA polymerase II Source: GO_Central
- tissue development Source: GO_Central
Keywordsi
Molecular function | Activator, DNA-binding, Receptor |
Biological process | Transcription, Transcription regulation |
Ligand | Lipid-binding, Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-MMU-383280, Nuclear Receptor transcription pathway R-MMU-4090294, SUMOylation of intracellular receptors R-MMU-9018519, Estrogen-dependent gene expression |
Names & Taxonomyi
Protein namesi | Recommended name: Nuclear receptor subfamily 5 group A member 2Alternative name(s): Liver receptor homolog 1 Short name: LRH-1 |
Gene namesi | Name:Nr5a2 Synonyms:Lrh1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1346834, Nr5a2 |
VEuPathDBi | HostDB:ENSMUSG00000026398 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Cytosol
- cytosol Source: Reactome
Nucleus
- nucleus Source: MGI
- RNA polymerase II transcription regulator complex Source: BHF-UCL
Other locations
- cytoplasm Source: MGI
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000053736 | 1 – 560 | Nuclear receptor subfamily 5 group A member 2Add BLAST | 560 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 289 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity |
Post-translational modificationi
Keywords - PTMi
Isopeptide bond, Ubl conjugationProteomic databases
MaxQBi | P45448 |
PaxDbi | P45448 |
PRIDEi | P45448 |
ProteomicsDBi | 293888 |
PTM databases
iPTMneti | P45448 |
PhosphoSitePlusi | P45448 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000026398, Expressed in female gonad and 199 other tissues |
Genevisiblei | P45448, MM |
Interactioni
Subunit structurei
Binds DNA as a monomer (By similarity).
Interacts with GRIP1, NCOA2 and NR0B2 (PubMed:12820970, PubMed:15976031).
Interacts (when sumoylated) with GPS2; interaction with GPS2 onto hepatic acute phase protein promoters prevents N-Cor corepressor complex dissociation (By similarity).
By similarity2 PublicationsGO - Molecular functioni
- transcription coregulator binding Source: Ensembl
Protein-protein interaction databases
BioGRIDi | 204976, 3 interactors |
STRINGi | 10090.ENSMUSP00000027649 |
Miscellaneous databases
RNActi | P45448, protein |
Structurei
Secondary structure
3D structure databases
SMRi | P45448 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P45448 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 319 – 558 | NR LBDPROSITE-ProRule annotationAdd BLAST | 240 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 21 – 55 | DisorderedSequence analysisAdd BLAST | 35 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 176 – 205 | FTZ-F1 boxAdd BLAST | 30 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 29 – 55 | Basic and acidic residuesSequence analysisAdd BLAST | 27 |
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 107 – 127 | NR C4-typePROSITE-ProRule annotationAdd BLAST | 21 | |
Zinc fingeri | 143 – 167 | NR C4-typePROSITE-ProRule annotationAdd BLAST | 25 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG4218, Eukaryota |
GeneTreei | ENSGT00940000153391 |
InParanoidi | P45448 |
OrthoDBi | 619653at2759 |
PhylomeDBi | P45448 |
TreeFami | TF350737 |
Family and domain databases
Gene3Di | 1.10.565.10, 1 hit 3.30.50.10, 1 hit |
IDEALi | IID50211 |
InterProi | View protein in InterPro IPR035500, NHR-like_dom_sf IPR016355, NR5_fam IPR000536, Nucl_hrmn_rcpt_lig-bd IPR001723, Nuclear_hrmn_rcpt IPR001628, Znf_hrmn_rcpt IPR013088, Znf_NHR/GATA |
PANTHERi | PTHR24086, PTHR24086, 1 hit |
Pfami | View protein in Pfam PF00104, Hormone_recep, 1 hit PF00105, zf-C4, 1 hit |
PIRSFi | PIRSF002530, Nuc_orph_FTZ-F1, 1 hit |
PRINTSi | PR00398, STRDHORMONER PR00047, STROIDFINGER |
SMARTi | View protein in SMART SM00430, HOLI, 1 hit SM00399, ZnF_C4, 1 hit |
SUPFAMi | SSF48508, SSF48508, 1 hit |
PROSITEi | View protein in PROSITE PS51843, NR_LBD, 1 hit PS00031, NUCLEAR_REC_DBD_1, 1 hit PS51030, NUCLEAR_REC_DBD_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MSASLDTGDF QEFLKHGLTA IASAPGSETR HSPKREEQLR EKRAGLPDRH
60 70 80 90 100
RRPIPARSRL VMLPKVETEA PGLVRSHGEQ GQMPENMQVS QFKMVNYSYD
110 120 130 140 150
EDLEELCPVC GDKVSGYHYG LLTCESCKGF FKRTVQNQKR YTCIENQNCQ
160 170 180 190 200
IDKTQRKRCP YCRFKKCIDV GMKLEAVRAD RMRGGRNKFG PMYKRDRALK
210 220 230 240 250
QQKKALIRAN GLKLEAMSQV IQAMPSDLTS AIQNIHSASK GLPLSHVALP
260 270 280 290 300
PTDYDRSPFV TSPISMTMPP HSSLHGYQPY GHFPSRAIKS EYPDPYSSSP
310 320 330 340 350
ESMMGYSYMD GYQTNSPASI PHLILELLKC EPDEPQVQAK IMAYLQQEQS
360 370 380 390 400
NRNRQEKLSA FGLLCKMADQ TLFSIVEWAR SSIFFRELKV DDQMKLLQNC
410 420 430 440 450
WSELLILDHI YRQVAHGKEG TIFLVTGEHV DYSTIISHTE VAFNNLLSLA
460 470 480 490 500
QELVVRLRSL QFDQREFVCL KFLVLFSSDV KNLENLQLVE GVQEQVNAAL
510 520 530 540 550
LDYTVCNYPQ QTEKFGQLLL RLPEIRAISK QAEDYLYYKH VNGDVPYNNL
560
LIEMLHAKRA
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ1WLP7 | Q1WLP7_MOUSE | Liver receptor-like protein 1 varia... | Nr5a2 | 499 | Annotation score: | ||
A0A0A6YY04 | A0A0A6YY04_MOUSE | Nuclear receptor subfamily 5 group ... | Nr5a2 | 539 | Annotation score: | ||
A0A0A6YWQ4 | A0A0A6YWQ4_MOUSE | Nuclear receptor subfamily 5 group ... | Nr5a2 | 136 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M81385 mRNA Translation: AAA39447.1 BC137845 mRNA Translation: AAI37846.1 |
CCDSi | CCDS15328.1 |
PIRi | S27874 |
RefSeqi | NP_109601.1, NM_030676.3 |
Genome annotation databases
Ensembli | ENSMUST00000027649; ENSMUSP00000027649; ENSMUSG00000026398 |
GeneIDi | 26424 |
KEGGi | mmu:26424 |
UCSCi | uc007cva.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M81385 mRNA Translation: AAA39447.1 BC137845 mRNA Translation: AAI37846.1 |
CCDSi | CCDS15328.1 |
PIRi | S27874 |
RefSeqi | NP_109601.1, NM_030676.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1PK5 | X-ray | 2.40 | A/B | 313-560 | [»] | |
1ZH7 | X-ray | 2.50 | A/B | 318-560 | [»] | |
3F5C | X-ray | 3.00 | A | 313-560 | [»] | |
SMRi | P45448 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 204976, 3 interactors |
STRINGi | 10090.ENSMUSP00000027649 |
PTM databases
iPTMneti | P45448 |
PhosphoSitePlusi | P45448 |
Proteomic databases
MaxQBi | P45448 |
PaxDbi | P45448 |
PRIDEi | P45448 |
ProteomicsDBi | 293888 |
Protocols and materials databases
Antibodypediai | 1690, 490 antibodies from 40 providers |
DNASUi | 26424 |
Genome annotation databases
Ensembli | ENSMUST00000027649; ENSMUSP00000027649; ENSMUSG00000026398 |
GeneIDi | 26424 |
KEGGi | mmu:26424 |
UCSCi | uc007cva.2, mouse |
Organism-specific databases
CTDi | 2494 |
MGIi | MGI:1346834, Nr5a2 |
VEuPathDBi | HostDB:ENSMUSG00000026398 |
Phylogenomic databases
eggNOGi | KOG4218, Eukaryota |
GeneTreei | ENSGT00940000153391 |
InParanoidi | P45448 |
OrthoDBi | 619653at2759 |
PhylomeDBi | P45448 |
TreeFami | TF350737 |
Enzyme and pathway databases
Reactomei | R-MMU-383280, Nuclear Receptor transcription pathway R-MMU-4090294, SUMOylation of intracellular receptors R-MMU-9018519, Estrogen-dependent gene expression |
Miscellaneous databases
BioGRID-ORCSi | 26424, 2 hits in 67 CRISPR screens |
ChiTaRSi | Nr5a2, mouse |
EvolutionaryTracei | P45448 |
PROi | PR:P45448 |
RNActi | P45448, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000026398, Expressed in female gonad and 199 other tissues |
Genevisiblei | P45448, MM |
Family and domain databases
Gene3Di | 1.10.565.10, 1 hit 3.30.50.10, 1 hit |
IDEALi | IID50211 |
InterProi | View protein in InterPro IPR035500, NHR-like_dom_sf IPR016355, NR5_fam IPR000536, Nucl_hrmn_rcpt_lig-bd IPR001723, Nuclear_hrmn_rcpt IPR001628, Znf_hrmn_rcpt IPR013088, Znf_NHR/GATA |
PANTHERi | PTHR24086, PTHR24086, 1 hit |
Pfami | View protein in Pfam PF00104, Hormone_recep, 1 hit PF00105, zf-C4, 1 hit |
PIRSFi | PIRSF002530, Nuc_orph_FTZ-F1, 1 hit |
PRINTSi | PR00398, STRDHORMONER PR00047, STROIDFINGER |
SMARTi | View protein in SMART SM00430, HOLI, 1 hit SM00399, ZnF_C4, 1 hit |
SUPFAMi | SSF48508, SSF48508, 1 hit |
PROSITEi | View protein in PROSITE PS51843, NR_LBD, 1 hit PS00031, NUCLEAR_REC_DBD_1, 1 hit PS51030, NUCLEAR_REC_DBD_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | NR5A2_MOUSE | |
Accessioni | P45448Primary (citable) accession number: P45448 Secondary accession number(s): B9EHF9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | May 30, 2000 | |
Last modified: | February 23, 2022 | |
This is version 185 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families