UniProtKB - P45442 (LAG2_CAEEL)
Protein
Protein lag-2
Gene
lag-2
Organism
Caenorhabditis elegans
Status
Functioni
Putative intercellular signal for lin-12 and GLP-1 receptors (PubMed:8139658). Functions in uterine cells to promote basement membrane mobility during tissue remodeling (PubMed:27661254).2 Publications
GO - Molecular functioni
- Notch binding Source: WormBase
GO - Biological processi
- cell communication Source: InterPro
- cell fate specification Source: WormBase
- gonad development Source: WormBase
- maintenance of dauer Source: WormBase
- nematode larval development Source: WormBase
- positive regulation of stem cell proliferation Source: WormBase
- regulation of vulval development Source: WormBase
Keywordsi
Molecular function | Developmental protein |
Biological process | Differentiation |
Enzyme and pathway databases
SignaLinki | P45442 |
Names & Taxonomyi
Protein namesi | Recommended name: Protein lag-2Alternative name(s): Lethal protein 461 |
Gene namesi | ORF Names:Y73C8B.4Imported |
Organismi | Caenorhabditis elegans |
Taxonomic identifieri | 6239 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditina › Rhabditomorpha › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis |
Proteomesi |
|
Organism-specific databases
WormBasei | Y73C8B.4 ; CE22970 ; WBGene00002246 ; lag-2 |
Subcellular locationi
Other locations
Plasma Membrane
- cell projection membrane Source: WormBase
- integral component of plasma membrane Source: WormBase
- neuronal cell body membrane Source: WormBase
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 16 – 279 | ExtracellularSequence analysisAdd BLAST | 264 | |
Transmembranei | 280 – 306 | HelicalSequence analysisAdd BLAST | 27 | |
Topological domaini | 307 – 402 | CytoplasmicSequence analysisAdd BLAST | 96 |
Keywords - Cellular componenti
MembranePathology & Biotechi
Disruption phenotypei
RNAi-mediated knockdown in cells of the pi uterine cell lineage results in impaired basement membrane mobility.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 262 | G → D in SA37; suppresses lin-12 function. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 15 | Sequence analysisAdd BLAST | 15 | |
ChainiPRO_0000007632 | 16 – 402 | Protein lag-2Add BLAST | 387 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 72 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 105 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 124 ↔ 133 | By similarity | ||
Disulfide bondi | 137 ↔ 149 | By similarity | ||
Disulfide bondi | 157 ↔ 166 | By similarity | ||
Disulfide bondi | 175 ↔ 183 | By similarity | ||
Disulfide bondi | 177 ↔ 204 | By similarity | ||
Glycosylationi | 194 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 206 ↔ 215 | By similarity | ||
Disulfide bondi | 233 ↔ 245 | By similarity | ||
Disulfide bondi | 239 ↔ 254 | By similarity | ||
Disulfide bondi | 256 ↔ 265 | By similarity |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
PaxDbi | P45442 |
Expressioni
Gene expression databases
Bgeei | WBGene00002246, Expressed in multi-cellular organism and 4 other tissues |
Interactioni
GO - Molecular functioni
- Notch binding Source: WormBase
Protein-protein interaction databases
BioGRIDi | 43812, 3 interactors |
DIPi | DIP-46051N |
IntActi | P45442, 1 interactor |
STRINGi | 6239.Y73C8B.4 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 122 – 166 | DSLPROSITE-ProRule annotationAdd BLAST | 45 | |
Domaini | 171 – 216 | EGF-like 1PROSITE-ProRule annotationAdd BLAST | 46 | |
Domaini | 229 – 266 | EGF-like 2PROSITE-ProRule annotationAdd BLAST | 38 |
Domaini
The DSL domain is required for binding to the Notch receptor.
Keywords - Domaini
EGF-like domain, Repeat, Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1218, Eukaryota |
GeneTreei | ENSGT00970000195885 |
HOGENOMi | CLU_693062_0_0_1 |
InParanoidi | P45442 |
OMAi | AVDPRKC |
OrthoDBi | 1103092at2759 |
PhylomeDBi | P45442 |
Family and domain databases
InterProi | View protein in InterPro IPR001774, DSL IPR000742, EGF-like_dom IPR039178, Lag2 |
PANTHERi | PTHR22669, PTHR22669, 1 hit |
Pfami | View protein in Pfam PF01414, DSL, 1 hit PF00008, EGF, 1 hit |
SMARTi | View protein in SMART SM00051, DSL, 1 hit SM00181, EGF, 2 hits |
PROSITEi | View protein in PROSITE PS51051, DSL, 1 hit PS00022, EGF_1, 2 hits PS01186, EGF_2, 2 hits PS50026, EGF_3, 2 hits |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P45442-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MIAYFLLLLT CLPVLQARVE VHQEFISSKR VSVRFEIVTE SHSPNRPVTF
60 70 80 90 100
DLFPRGPKTN IILLDTFNPV FNFSIQLVQP FTGQPLGDRI YRKVQFSGTN
110 120 130 140 150
QPWINDTFTT TSGISLSVAT EVTCARNYFG NRCENFCDAH LAKAARKRCD
160 170 180 190 200
AMGRLRCDIG WMGPHCGQAV DPRKCSCEND GICVSSMIHP SQPNQTSSNE
210 220 230 240 250
QLICECTNGF TGTRCEIFGF NQFQLTAPRP DACSVKDACL NGAKCFPNGP
260 270 280 290 300
KVFCSCAVGF IGEFCEISLT TTTPTTVEIT VSTSGYSSAV YITVALFVIF
310 320 330 340 350
SIIIGCFKYK FKPMRQQALA RGQVPEPYKM PETKSMLIDP EASEAQKKVF
360 370 380 390 400
TIEGSVQKID EEVRYTSAPR KYESNNEYAV IQKSTPPPPS LSPPSIPACH
YV
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X77495 Genomic DNA Translation: CAA54629.1 BX284605 Genomic DNA Translation: CCD66873.1 |
PIRi | S42367 |
RefSeqi | NP_503877.1, NM_071476.1 |
Genome annotation databases
EnsemblMetazoai | Y73C8B.4.1; Y73C8B.4.1; WBGene00002246 |
GeneIDi | 178755 |
KEGGi | cel:CELE_Y73C8B.4 |
UCSCi | Y73C8B.4, c. elegans |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X77495 Genomic DNA Translation: CAA54629.1 BX284605 Genomic DNA Translation: CCD66873.1 |
PIRi | S42367 |
RefSeqi | NP_503877.1, NM_071476.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 43812, 3 interactors |
DIPi | DIP-46051N |
IntActi | P45442, 1 interactor |
STRINGi | 6239.Y73C8B.4 |
Proteomic databases
PaxDbi | P45442 |
Genome annotation databases
EnsemblMetazoai | Y73C8B.4.1; Y73C8B.4.1; WBGene00002246 |
GeneIDi | 178755 |
KEGGi | cel:CELE_Y73C8B.4 |
UCSCi | Y73C8B.4, c. elegans |
Organism-specific databases
CTDi | 178755 |
WormBasei | Y73C8B.4 ; CE22970 ; WBGene00002246 ; lag-2 |
Phylogenomic databases
eggNOGi | KOG1218, Eukaryota |
GeneTreei | ENSGT00970000195885 |
HOGENOMi | CLU_693062_0_0_1 |
InParanoidi | P45442 |
OMAi | AVDPRKC |
OrthoDBi | 1103092at2759 |
PhylomeDBi | P45442 |
Enzyme and pathway databases
SignaLinki | P45442 |
Miscellaneous databases
PROi | PR:P45442 |
Gene expression databases
Bgeei | WBGene00002246, Expressed in multi-cellular organism and 4 other tissues |
Family and domain databases
InterProi | View protein in InterPro IPR001774, DSL IPR000742, EGF-like_dom IPR039178, Lag2 |
PANTHERi | PTHR22669, PTHR22669, 1 hit |
Pfami | View protein in Pfam PF01414, DSL, 1 hit PF00008, EGF, 1 hit |
SMARTi | View protein in SMART SM00051, DSL, 1 hit SM00181, EGF, 2 hits |
PROSITEi | View protein in PROSITE PS51051, DSL, 1 hit PS00022, EGF_1, 2 hits PS01186, EGF_2, 2 hits PS50026, EGF_3, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | LAG2_CAEEL | |
Accessioni | P45442Primary (citable) accession number: P45442 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | November 1, 1995 | |
Last modified: | April 7, 2021 | |
This is version 136 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Caenorhabditis annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Caenorhabditis elegans
Caenorhabditis elegans: entries, gene names and cross-references to WormBase