UniProtKB - P45243 (SPPA_HAEIN)
Protein
Protease 4
Gene
sppA
Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Functioni
Digests cleaved signal peptides in vitro, its in vivo function is unknown. This activity is necessary to maintain proper secretion of mature proteins across the membrane (By similarity).By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 201 | Proton donor/acceptorBy similarity | 1 | |
Active sitei | 405 | NucleophileBy similarity | 1 |
GO - Molecular functioni
- serine-type peptidase activity Source: UniProtKB-KW
GO - Biological processi
- signal peptide processing Source: InterPro
Keywordsi
Molecular function | Hydrolase, Protease, Serine protease |
Enzyme and pathway databases
BioCyci | HINF71421:G1GJ1-1561-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Protease 4 (EC:3.4.21.-)Alternative name(s): Endopeptidase IV Protease IV Signal peptide peptidase |
Gene namesi | Name:sppA Ordered Locus Names:HI_1541 |
Organismi | Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) |
Taxonomic identifieri | 71421 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus › |
Proteomesi |
|
Subcellular locationi
- Cell inner membrane By similarity; Single-pass membrane protein By similarity
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 17 | CytoplasmicSequence analysisAdd BLAST | 17 | |
Transmembranei | 18 – 38 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 39 – 615 | PeriplasmicSequence analysisAdd BLAST | 577 |
GO - Cellular componenti
- integral component of membrane Source: UniProtKB-KW
- plasma membrane Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000171439 | 1 – 615 | Protease 4Add BLAST | 615 |
Interactioni
Subunit structurei
Homotetramer.
By similarityProtein-protein interaction databases
STRINGi | 71421.HI_1541 |
Family & Domainsi
Sequence similaritiesi
Belongs to the peptidase S49 family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG0616, Bacteria |
HOGENOMi | CLU_008856_1_1_6 |
OMAi | DPKGQYL |
PhylomeDBi | P45243 |
Family and domain databases
CDDi | cd07023, S49_Sppa_N_C, 1 hit |
InterProi | View protein in InterPro IPR029045, ClpP/crotonase-like_dom_sf IPR004634, Pept_S49_pIV IPR004635, Pept_S49_SppA IPR002142, Peptidase_S49 |
Pfami | View protein in Pfam PF01343, Peptidase_S49, 2 hits |
PIRSFi | PIRSF001217, Protease_4_SppA, 1 hit |
SUPFAMi | SSF52096, SSF52096, 2 hits |
TIGRFAMsi | TIGR00705, SppA_67K, 1 hit TIGR00706, SppA_dom, 1 hit |
i Sequence
Sequence statusi: Complete.
P45243-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MFQVLKFCWK VLCFIRDLVM NVVFLGFVLL LVAIISFSSG GKKSTALTSE
60 70 80 90 100
GALLLNLDGY LADNRDETLR WQDALSELNG EHVPRKISTF DVVFAIQQAE
110 120 130 140 150
DDPKIKGLVL DLNYFEGADL PALDFIGGAI SHFKDAGKPV IAYADNYSQG
160 170 180 190 200
QYYLASFADE IYLNSIGSVD IHGLSQENLY FKEMLDKLAV TPHIFRVGTY
210 220 230 240 250
KSAVEPFLRN DMSAEAKANM QRWLGEMWNN YVLSVSENRN IKKDRILPNA
260 270 280 290 300
KQYLAELKAL KGNSTAYAQQ RGLVTDVVTR LDLDKKLSAL FGKGSDGKAN
310 320 330 340 350
LIEFDDYLTQ LPDRLEHYNV PNKIAVVNVE GTIIDGESDE ENAGGDTIAR
360 370 380 390 400
ILRKAHDDNS VKAVILRVNS PGGSAFASEI IRQETENLQK IGKPVIVSMG
410 420 430 440 450
AMAASGGYWI SSTADYIIAD SNTITGSIGI FTMFPTFENS IKKIGVHADG
460 470 480 490 500
VSTTELANTS AFSPLAKPVQ DIYQTEIEHG YDRFLEIVSK GRQLSKTQVD
510 520 530 540 550
KLAQGQVWLG SDAFQNGLVD EIGSFNEAVN KAEQLVNQRQ DTAVQDFSVE
560 570 580 590 600
WFTDDNVSLI STLLSDTKKG AQEQLVKWLG LPAPIQKLQK ELNILTKFND
610
PKGQYLYCLN CGKVK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L42023 Genomic DNA Translation: AAC23191.1 |
PIRi | F64128 |
RefSeqi | NP_439690.1, NC_000907.1 WP_005693566.1, NC_000907.1 |
Genome annotation databases
EnsemblBacteriai | AAC23191; AAC23191; HI_1541 |
KEGGi | hin:HI1541 |
PATRICi | fig|71421.8.peg.1612 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L42023 Genomic DNA Translation: AAC23191.1 |
PIRi | F64128 |
RefSeqi | NP_439690.1, NC_000907.1 WP_005693566.1, NC_000907.1 |
3D structure databases
SMRi | P45243 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 71421.HI_1541 |
Genome annotation databases
EnsemblBacteriai | AAC23191; AAC23191; HI_1541 |
KEGGi | hin:HI1541 |
PATRICi | fig|71421.8.peg.1612 |
Phylogenomic databases
eggNOGi | COG0616, Bacteria |
HOGENOMi | CLU_008856_1_1_6 |
OMAi | DPKGQYL |
PhylomeDBi | P45243 |
Enzyme and pathway databases
BioCyci | HINF71421:G1GJ1-1561-MONOMER |
Family and domain databases
CDDi | cd07023, S49_Sppa_N_C, 1 hit |
InterProi | View protein in InterPro IPR029045, ClpP/crotonase-like_dom_sf IPR004634, Pept_S49_pIV IPR004635, Pept_S49_SppA IPR002142, Peptidase_S49 |
Pfami | View protein in Pfam PF01343, Peptidase_S49, 2 hits |
PIRSFi | PIRSF001217, Protease_4_SppA, 1 hit |
SUPFAMi | SSF52096, SSF52096, 2 hits |
TIGRFAMsi | TIGR00705, SppA_67K, 1 hit TIGR00706, SppA_dom, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SPPA_HAEIN | |
Accessioni | P45243Primary (citable) accession number: P45243 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | November 1, 1995 | |
Last modified: | December 2, 2020 | |
This is version 127 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - SIMILARITY comments
Index of protein domains and families