UniProtKB - P44969 (IGA0_HAEIN)
Protein
Immunoglobulin A1 protease autotransporter
Gene
iga
Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Functioni
Virulence factor; cleaves host immunoglobulin A producing intact Fc and Fab fragments.
Catalytic activityi
- Cleavage of immunoglobulin A molecules at certain Pro-|-Xaa bonds in the hinge region. No small molecule substrates are known. EC:3.4.21.72
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 288 | Curated | 1 |
GO - Molecular functioni
- serine-type endopeptidase activity Source: InterPro
GO - Biological processi
- pathogenesis Source: UniProtKB-KW
Keywordsi
Molecular function | Hydrolase, Protease, Serine protease |
Biological process | Virulence |
Enzyme and pathway databases
BioCyci | HINF71421:G1GJ1-1032-MONOMER |
BRENDAi | 3.4.21.72, 2529 |
Protein family/group databases
MEROPSi | S06.007 |
Names & Taxonomyi
Protein namesi | Recommended name: Immunoglobulin A1 protease autotransporter (EC:3.4.21.72)Cleaved into the following 2 chains: Alternative name(s): Helper peptide |
Gene namesi | Name:iga Synonyms:iga1 Ordered Locus Names:HI_0990 |
Organismi | Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) |
Taxonomic identifieri | 71421 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus › |
Proteomesi |
|
Subcellular locationi
Other locations
- Periplasm By similarity
Extracellular region or secreted
Other locations
Other locations
- Cell outer membrane By similarity; Multi-pass membrane protein By similarity
Note: The cleaved C-terminal fragment (autotransporter domain) is localized in the outer membrane.By similarity
Extracellular region or secreted
- extracellular region Source: UniProtKB-SubCell
Other locations
- cell outer membrane Source: UniProtKB-SubCell
- cell surface Source: UniProtKB-SubCell
- integral component of membrane Source: UniProtKB-KW
- periplasmic space Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell outer membrane, Membrane, Periplasm, SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 25 | Sequence analysisAdd BLAST | 25 | |
ChainiPRO_0000387598 | 26 – 1694 | Immunoglobulin A1 protease autotransporterAdd BLAST | 1669 | |
ChainiPRO_0000026958 | 26 – 1014 | Immunoglobulin A1 proteaseAdd BLAST | 989 | |
ChainiPRO_0000026959 | 1015 – 1694 | Immunoglobulin A1 protease translocatorSequence analysisAdd BLAST | 680 |
Keywords - PTMi
ZymogenStructurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P44969 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P44969 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 26 – 332 | Peptidase S6PROSITE-ProRule annotationAdd BLAST | 307 | |
Domaini | 1442 – 1694 | AutotransporterPROSITE-ProRule annotationAdd BLAST | 253 |
Domaini
The signal peptide, cleaved at the inner membrane, guides the autotransporter protein to the periplasmic space. Then, insertion of the C-terminal translocator domain in the outer membrane forms a hydrophilic pore for the translocation of the passenger domain to the bacterial cell surface, with subsequent cleavage (By similarity).By similarity
Keywords - Domaini
Signal, Transmembrane, Transmembrane beta strandPhylogenomic databases
eggNOGi | COG3266, Bacteria COG3468, Bacteria |
HOGENOMi | CLU_004023_0_0_6 |
OMAi | AAPQDYM |
Family and domain databases
Gene3Di | 2.160.20.20, 1 hit 2.40.128.130, 1 hit |
InterProi | View protein in InterPro IPR005546, Autotransporte_beta IPR036709, Autotransporte_beta_dom_sf IPR012332, P22_tailspike-like_C_sf IPR011050, Pectin_lyase_fold/virulence IPR000710, Peptidase_S6 IPR030396, Peptidase_S6_dom IPR004899, Pertactin_central |
Pfami | View protein in Pfam PF03797, Autotransporter, 1 hit PF02395, Peptidase_S6, 1 hit PF03212, Pertactin, 1 hit |
PRINTSi | PR00921, IGASERPTASE |
SMARTi | View protein in SMART SM00869, Autotransporter, 1 hit |
SUPFAMi | SSF103515, SSF103515, 1 hit SSF51126, SSF51126, 1 hit |
PROSITEi | View protein in PROSITE PS51208, AUTOTRANSPORTER, 1 hit PS51691, PEPTIDASE_S6, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P44969-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLNKKFKLNF IALTVAYALT PYTEAALVRD DVDYQIFRDF AENKGRFSVG
60 70 80 90 100
ATNVEVRDKN NHSLGNVLPN GIPMIDFSVV DVDKRIATLI NPQYVVGVKH
110 120 130 140 150
VSNGVSELHF GNLNGNMNNG NAKSHRDVSS EENRYFSVEK NEYPTKLNGK
160 170 180 190 200
AVTTEDQTQK RREDYYMPRL DKFVTEVAPI EASTASSDAG TYNDQNKYPA
210 220 230 240 250
FVRLGSGSQF IYKKGDNYSL ILNNHEVGGN NLKLVGDAYT YGIAGTPYKV
260 270 280 290 300
NHENNGLIGF GNSKEEHSDP KGILSQDPLT NYAVLGDSGS PLFVYDREKG
310 320 330 340 350
KWLFLGSYDF WAGYNKKSWQ EWNIYKPEFA KTVLDKDTAG SLTGSNTQYN
360 370 380 390 400
WNPTGKTSVI SNGSESLNVD LFDSSQDTDS KKNNHGKSVT LRGSGTLTLN
410 420 430 440 450
NNIDQGAGGL FFEGDYEVKG TSDSTTWKGA GVSVADGKTV TWKVHNPKSD
460 470 480 490 500
RLAKIGKGTL IVEGKGENKG SLKVGDGTVI LKQQADANNK VKAFSQVGIV
510 520 530 540 550
SGRSTVVLND DKQVDPNSIY FGFRGGRLDA NGNNLTFEHI RNIDDGARLV
560 570 580 590 600
NHNTSKTSTV TITGESLITD PNTITPYNID APDEDNPYAF RRIKDGGQLY
610 620 630 640 650
LNLENYTYYA LRKGASTRSE LPKNSGESNE NWLYMGKTSD EAKRNVMNHI
660 670 680 690 700
NNERMNGFNG YFGEEEGKNN GNLNVTFKGK SEQNRFLLTG GTNLNGDLKV
710 720 730 740 750
EKGTLFLSGR PTPHARDIAG ISSTKKDQHF AENNEVVVED DWINRNFKAT
760 770 780 790 800
NINVTNNATL YSGRNVANIT SNITASDNAK VHIGYKAGDT VCVRSDYTGY
810 820 830 840 850
VTCTTDKLSD KALNSFNATN VSGNVNLSGN ANFVLGKANL FGTISGTGNS
860 870 880 890 900
QVRLTENSHW HLTGDSNVNQ LNLDKGHIHL NAQNDANKVT TYNTLTVNSL
910 920 930 940 950
SGNGSFYYLT DLSNKQGDKV VVTKSATGNF TLQVADKTGE PTKNELTLFD
960 970 980 990 1000
ASNATRNNLN VSLVGNTVDL GAWKYKLRNV NGRYDLYNPE VEKRNQTVDT
1010 1020 1030 1040 1050
TNITTPNNIQ ADVPSVPSNN EEIARVETPV PPPAPATPSE TTETVAENSK
1060 1070 1080 1090 1100
QESKTVEKNE QDATETTAQN GEVAEEAKPS VKANTQTNEV AQSGSETEET
1110 1120 1130 1140 1150
QTTEIKETAK VEKEEKAKVE KDEIQEAPQM ASETSPKQAK PAPKEVSTDT
1160 1170 1180 1190 1200
KVEETQVQAQ PQTQSTTVAA AEATSPNSKP AEETQPSEKT NAEPVTPVVS
1210 1220 1230 1240 1250
KNQTENTTDQ PTEREKTAKV ETEKTQEPPQ VASQASPKQE QSETVQPQAV
1260 1270 1280 1290 1300
LESENVPTVN NAEEVQAQLQ TQTSATVSTK QPAPENSINT GSATAITETA
1310 1320 1330 1340 1350
EKSDKPQTET AASTEDASQH KANTVADNSV ANNSESSDPK SRRRRSISQP
1360 1370 1380 1390 1400
QETSAEETTA ASTDETTIAD NSKRSKPNRR SRRSVRSEPT VTNGSDRSTV
1410 1420 1430 1440 1450
ALRDLTSTNT NAVISDAMAK AQFVALNVGK AVSQHISQLE MNNEGQYNVW
1460 1470 1480 1490 1500
VSNTSMNENY SSSQYRRFSS KSTQTQLGWD QTISNNVQLG GVFTYVRNSN
1510 1520 1530 1540 1550
NFDKASSKNT LAQVNFYSKY YADNHWYLGI DLGYGKFQSN LKTNHNAKFA
1560 1570 1580 1590 1600
RHTAQFGLTA GKAFNLGNFG ITPIVGVRYS YLSNANFALA KDRIKVNPIS
1610 1620 1630 1640 1650
VKTAFAQVDL SYTYHLGEFS VTPILSARYD TNQGSGKINV NQYDFAYNVE
1660 1670 1680 1690
NQQQYNAGLK LKYHNVKLSL IGGLTKAKQA EKQKTAELKL SFSF
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 253 – 254 | EN → GV in CAB56789 (Ref. 1) Curated | 2 | |
Sequence conflicti | 272 | G → A in CAB56789 (Ref. 1) Curated | 1 | |
Sequence conflicti | 464 | G → E in CAB56789 (Ref. 1) Curated | 1 | |
Sequence conflicti | 866 | S → T in CAB56789 (Ref. 1) Curated | 1 | |
Sequence conflicti | 1036 | A → D in CAB56789 (Ref. 1) Curated | 1 | |
Sequence conflicti | 1074 | A → G in CAB56789 (Ref. 1) Curated | 1 | |
Sequence conflicti | 1421 | A → G in CAB56789 (Ref. 1) Curated | 1 | |
Sequence conflicti | 1545 | H → T in CAB56789 (Ref. 1) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X59800 Genomic DNA Translation: CAB56789.1 L42023 Genomic DNA Translation: AAC22651.1 |
PIRi | H64106 |
RefSeqi | NP_439153.1, NC_000907.1 WP_005693332.1, NC_000907.1 |
Genome annotation databases
EnsemblBacteriai | AAC22651; AAC22651; HI_0990 |
KEGGi | hin:HI_0990 |
PATRICi | fig|71421.8.peg.1033 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X59800 Genomic DNA Translation: CAB56789.1 L42023 Genomic DNA Translation: AAC22651.1 |
PIRi | H64106 |
RefSeqi | NP_439153.1, NC_000907.1 WP_005693332.1, NC_000907.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3H09 | X-ray | 1.75 | A/B | 26-1014 | [»] | |
SMRi | P44969 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 71421.HI_0990 |
Protein family/group databases
MEROPSi | S06.007 |
Genome annotation databases
EnsemblBacteriai | AAC22651; AAC22651; HI_0990 |
KEGGi | hin:HI_0990 |
PATRICi | fig|71421.8.peg.1033 |
Phylogenomic databases
eggNOGi | COG3266, Bacteria COG3468, Bacteria |
HOGENOMi | CLU_004023_0_0_6 |
OMAi | AAPQDYM |
Enzyme and pathway databases
BioCyci | HINF71421:G1GJ1-1032-MONOMER |
BRENDAi | 3.4.21.72, 2529 |
Miscellaneous databases
EvolutionaryTracei | P44969 |
Family and domain databases
Gene3Di | 2.160.20.20, 1 hit 2.40.128.130, 1 hit |
InterProi | View protein in InterPro IPR005546, Autotransporte_beta IPR036709, Autotransporte_beta_dom_sf IPR012332, P22_tailspike-like_C_sf IPR011050, Pectin_lyase_fold/virulence IPR000710, Peptidase_S6 IPR030396, Peptidase_S6_dom IPR004899, Pertactin_central |
Pfami | View protein in Pfam PF03797, Autotransporter, 1 hit PF02395, Peptidase_S6, 1 hit PF03212, Pertactin, 1 hit |
PRINTSi | PR00921, IGASERPTASE |
SMARTi | View protein in SMART SM00869, Autotransporter, 1 hit |
SUPFAMi | SSF103515, SSF103515, 1 hit SSF51126, SSF51126, 1 hit |
PROSITEi | View protein in PROSITE PS51208, AUTOTRANSPORTER, 1 hit PS51691, PEPTIDASE_S6, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | IGA0_HAEIN | |
Accessioni | P44969Primary (citable) accession number: P44969 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | November 1, 1995 | |
Last modified: | April 7, 2021 | |
This is version 141 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references