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UniProtKB - P44774 (SHDHL_HAEIN)
Protein
Shikimate dehydrogenase-like protein HI_0607
Gene
sdhL
Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Functioni
In vitro, is able to catalyze the NADP+-dependent oxidation of shikimate to 3-dehydroshikimate. However, has much lower activity than classical shikimate dehydrogenases AroE, indicating that shikimate may not be the biological substrate. Cannot utilize NAD+ instead of NADP+. Is not able to catalyze the oxidation of quinate.
1 PublicationCatalytic activityi
- EC:1.1.1.251 Publication
Kineticsi
kcat is 0.2 sec(-1).1 Publication
- KM=234 µM for shikimate1 Publication
- KM=234 µM for NADP+1 Publication
pH dependencei
Optimum pH is 8.8.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 67 | Proton donor/acceptor1 Publication | 1 | |
Binding sitei | 103 | Substrate1 Publication | 1 | |
Binding sitei | 154 | NADPBy similarity | 1 | |
Binding sitei | 184 | NADPBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 126 – 130 | NADPBy similarity | 5 |
GO - Molecular functioni
- NADP binding Source: GO_Central
- shikimate 3-dehydrogenase (NADP+) activity Source: GO_Central
GO - Biological processi
- chorismate biosynthetic process Source: GO_Central
- shikimate metabolic process Source: GO_Central
Keywordsi
Molecular function | Oxidoreductase |
Ligand | NADP |
Enzyme and pathway databases
BRENDAi | 1.1.1.282, 2529 |
Names & Taxonomyi
Protein namesi | Recommended name: Shikimate dehydrogenase-like protein HI_06071 Publication (EC:1.1.1.251 Publication)Short name: SDH-L1 Publication |
Gene namesi | Name:sdhL1 Publication Ordered Locus Names:HI_0607 |
Organismi | Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) |
Taxonomic identifieri | 71421 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus › |
Proteomesi |
|
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 67 | K → A, H or N: Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 103 | D → A or N: Loss of activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000136073 | 1 – 271 | Shikimate dehydrogenase-like protein HI_0607Add BLAST | 271 |
Interactioni
Subunit structurei
Homodimer.
1 PublicationProtein-protein interaction databases
STRINGi | 71421.HI_0607 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P44774 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P44774 |
Family & Domainsi
Sequence similaritiesi
Belongs to the shikimate dehydrogenase-like family.2 Publications
Phylogenomic databases
eggNOGi | COG0169, Bacteria |
HOGENOMi | CLU_044063_3_0_6 |
OMAi | MPFKESC |
PhylomeDBi | P44774 |
Family and domain databases
InterProi | View protein in InterPro IPR036291, NAD(P)-bd_dom_sf IPR013708, Shikimate_DH-bd_N IPR022893, Shikimate_DH_fam |
PANTHERi | PTHR21089, PTHR21089, 1 hit |
Pfami | View protein in Pfam PF08501, Shikimate_dh_N, 1 hit |
SUPFAMi | SSF51735, SSF51735, 1 hit |
i Sequence
Sequence statusi: Complete.
P44774-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MINKDTQLCM SLSGRPSNFG TTFHNYLYDK LGLNFIYKAF TTQDIEHAIK
60 70 80 90 100
GVRALGIRGC AVSMPFKETC MPFLDEIHPS AQAIESVNTI VNDNGFLRAY
110 120 130 140 150
NTDYIAIVKL IEKYHLNKNA KVIVHGSGGM AKAVVAAFKN SGFEKLKIYA
160 170 180 190 200
RNVKTGQYLA ALYGYAYINS LENQQADILV NVTSIGMKGG KEEMDLAFPK
210 220 230 240 250
AFIDNASVAF DVVAMPVETP FIRYAQARGK QTISGAAVIV LQAVEQFELY
260 270
THQRPSDELI AEAAAFARTK F
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L42023 Genomic DNA Translation: AAC22266.1 |
PIRi | H64080 |
RefSeqi | NP_438765.1, NC_000907.1 WP_005694543.1, NC_000907.1 |
Genome annotation databases
EnsemblBacteriai | AAC22266; AAC22266; HI_0607 |
KEGGi | hin:HI_0607 |
PATRICi | fig|71421.8.peg.631 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L42023 Genomic DNA Translation: AAC22266.1 |
PIRi | H64080 |
RefSeqi | NP_438765.1, NC_000907.1 WP_005694543.1, NC_000907.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1NPY | X-ray | 1.75 | A/B/C/D | 1-271 | [»] | |
SMRi | P44774 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 71421.HI_0607 |
Genome annotation databases
EnsemblBacteriai | AAC22266; AAC22266; HI_0607 |
KEGGi | hin:HI_0607 |
PATRICi | fig|71421.8.peg.631 |
Phylogenomic databases
eggNOGi | COG0169, Bacteria |
HOGENOMi | CLU_044063_3_0_6 |
OMAi | MPFKESC |
PhylomeDBi | P44774 |
Enzyme and pathway databases
BRENDAi | 1.1.1.282, 2529 |
Miscellaneous databases
EvolutionaryTracei | P44774 |
Family and domain databases
InterProi | View protein in InterPro IPR036291, NAD(P)-bd_dom_sf IPR013708, Shikimate_DH-bd_N IPR022893, Shikimate_DH_fam |
PANTHERi | PTHR21089, PTHR21089, 1 hit |
Pfami | View protein in Pfam PF08501, Shikimate_dh_N, 1 hit |
SUPFAMi | SSF51735, SSF51735, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | SHDHL_HAEIN | |
Accessioni | P44774Primary (citable) accession number: P44774 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | November 1, 1995 | |
Last modified: | February 23, 2022 | |
This is version 131 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families