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UniProtKB - P44539 (NANA_HAEIN)
Protein
N-acetylneuraminate lyase
Gene
nanA
Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Functioni
Catalyzes the reversible aldol cleavage of N-acetylneuraminic acid (sialic acid; Neu5Ac) to form pyruvate and N-acetylmannosamine (ManNAc) via a Schiff base intermediate.
Catalytic activityi
- EC:4.1.3.3
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 136 | Involved in proton transfer during cleavage | 1 | |
Active sitei | 164 | Schiff-base intermediate with substrate | 1 |
GO - Molecular functioni
- N-acetylneuraminate lyase activity Source: GO_Central
GO - Biological processi
- carbohydrate metabolic process Source: UniProtKB-UniRule
- N-acetylneuraminate catabolic process Source: GO_Central
Keywordsi
Molecular function | Lyase |
Biological process | Carbohydrate metabolism |
Ligand | Schiff base |
Names & Taxonomyi
Protein namesi | Recommended name: N-acetylneuraminate lyase (EC:4.1.3.3)Short name: NAL Short name: Neu5Ac lyase Alternative name(s): N-acetylneuraminate pyruvate-lyase N-acetylneuraminic acid aldolase Sialate lyase Sialic acid aldolase Sialic acid lyase |
Gene namesi | Name:nanA Ordered Locus Names:HI_0142 |
Organismi | Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) |
Taxonomic identifieri | 71421 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
Cytosol
- cytosol Source: GO_Central
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000103214 | 1 – 293 | N-acetylneuraminate lyaseAdd BLAST | 293 |
Interactioni
Subunit structurei
Homotetramer.
Protein-protein interaction databases
STRINGi | 71421.HI_0142 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | P44539 |
SMRi | P44539 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P44539 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 47 – 48 | Substrate bindingBy similarity | 2 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0329, Bacteria |
HOGENOMi | CLU_049343_6_0_6 |
OMAi | VVPPVCT |
PhylomeDBi | P44539 |
Family and domain databases
CDDi | cd00954, NAL, 1 hit |
Gene3Di | 3.20.20.70, 1 hit |
HAMAPi | MF_01237, N_acetylneuram_lyase, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR002220, DapA-like IPR005264, NanA IPR020625, Schiff_base-form_aldolases_AS IPR020624, Schiff_base-form_aldolases_CS |
Pfami | View protein in Pfam PF00701, DHDPS, 1 hit |
PIRSFi | PIRSF001365, DHDPS, 1 hit |
PRINTSi | PR00146, DHPICSNTHASE |
SMARTi | View protein in SMART SM01130, DHDPS, 1 hit |
TIGRFAMsi | TIGR00683, nanA, 1 hit |
PROSITEi | View protein in PROSITE PS00665, DHDPS_1, 1 hit PS00666, DHDPS_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P44539-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRDLKGIFSA LLVSFNEDGT INEKGLRQII RHNIDKMKVD GLYVGGSTGE
60 70 80 90 100
NFMLSTEEKK EIFRIAKDEA KDQIALIAQV GSVNLKEAVE LGKYATELGY
110 120 130 140 150
DCLSAVTPFY YKFSFPEIKH YYDTIIAETG NNMIVYSIPF LTGVNMGIEQ
160 170 180 190 200
FGELYKNPKV LGVKFTAGDF YLLERLKKAY PNHLIWAGFD EMMLPAASLG
210 220 230 240 250
VDGAIGSTFN VNGVRARQIF ELTKAGKLAE ALEIQHVTND LIEGILANGL
260 270 280 290
YLTIKELLKL EGVDAGYCRE PMTSKATEEQ LAKAKDLKAK FLS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L42023 Genomic DNA Translation: AAC21814.1 |
PIRi | G64050 |
RefSeqi | NP_438311.1, NC_000907.1 WP_005694428.1, NC_000907.1 |
Genome annotation databases
EnsemblBacteriai | AAC21814; AAC21814; HI_0142 |
KEGGi | hin:HI_0142 |
PATRICi | fig|71421.8.peg.144 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L42023 Genomic DNA Translation: AAC21814.1 |
PIRi | G64050 |
RefSeqi | NP_438311.1, NC_000907.1 WP_005694428.1, NC_000907.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1F5Z | X-ray | 1.88 | A/B/C/D | 1-293 | [»] | |
1F6K | X-ray | 1.60 | A/C | 1-293 | [»] | |
1F6P | X-ray | 2.25 | A/B/C/D | 1-293 | [»] | |
1F73 | X-ray | 1.95 | A/B/C/D | 1-293 | [»] | |
1F74 | X-ray | 1.60 | A/C | 1-293 | [»] | |
1F7B | X-ray | 1.80 | A/C | 1-293 | [»] | |
AlphaFoldDBi | P44539 | |||||
SMRi | P44539 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 71421.HI_0142 |
Genome annotation databases
EnsemblBacteriai | AAC21814; AAC21814; HI_0142 |
KEGGi | hin:HI_0142 |
PATRICi | fig|71421.8.peg.144 |
Phylogenomic databases
eggNOGi | COG0329, Bacteria |
HOGENOMi | CLU_049343_6_0_6 |
OMAi | VVPPVCT |
PhylomeDBi | P44539 |
Miscellaneous databases
EvolutionaryTracei | P44539 |
PROi | PR:P44539 |
Family and domain databases
CDDi | cd00954, NAL, 1 hit |
Gene3Di | 3.20.20.70, 1 hit |
HAMAPi | MF_01237, N_acetylneuram_lyase, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR002220, DapA-like IPR005264, NanA IPR020625, Schiff_base-form_aldolases_AS IPR020624, Schiff_base-form_aldolases_CS |
Pfami | View protein in Pfam PF00701, DHDPS, 1 hit |
PIRSFi | PIRSF001365, DHDPS, 1 hit |
PRINTSi | PR00146, DHPICSNTHASE |
SMARTi | View protein in SMART SM01130, DHDPS, 1 hit |
TIGRFAMsi | TIGR00683, nanA, 1 hit |
PROSITEi | View protein in PROSITE PS00665, DHDPS_1, 1 hit PS00666, DHDPS_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | NANA_HAEIN | |
Accessioni | P44539Primary (citable) accession number: P44539 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | November 1, 1995 | |
Last modified: | May 25, 2022 | |
This is version 136 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families