UniProtKB - P44414 (MTHD_HAEIN)
Protein
Probable modification methylase HindII
Gene
hindIIM
Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Functioni
This methylase recognizes the double-stranded sequence GTYRAC, causes specific methylation on A-5 on both strands, and protects the DNA from cleavage by the HindII endonuclease.
Catalytic activityi
- a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an N6-methyl-2'-deoxyadenosine in DNA + H+ + S-adenosyl-L-homocysteineEC:2.1.1.72
GO - Molecular functioni
- DNA binding Source: InterPro
- N-methyltransferase activity Source: InterPro
- site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC
GO - Biological processi
- DNA restriction-modification system Source: UniProtKB-KW
Keywordsi
Molecular function | Methyltransferase, Transferase |
Biological process | Restriction system |
Ligand | S-adenosyl-L-methionine |
Enzyme and pathway databases
BioCyci | HINF71421:G1GJ1-526-MONOMER |
Protein family/group databases
REBASEi | 3427, M.HindII |
Names & Taxonomyi
Protein namesi | Recommended name: Probable modification methylase HindII (EC:2.1.1.72)Short name: M.HindII Alternative name(s): Adenine-specific methyltransferase HindII |
Gene namesi | Name:hindIIM Ordered Locus Names:HI_0513 |
Organismi | Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) |
Taxonomic identifieri | 71421 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000087974 | 1 – 518 | Probable modification methylase HindIIAdd BLAST | 518 |
Family & Domainsi
Sequence similaritiesi
Belongs to the N(4)/N(6)-methyltransferase family.Curated
Phylogenomic databases
eggNOGi | COG0827, Bacteria |
HOGENOMi | CLU_508657_0_0_6 |
Family and domain databases
InterProi | View protein in InterPro IPR003356, DNA_methylase_A-5 IPR002052, DNA_methylase_N6_adenine_CS IPR011639, RM_methylase_Eco57I IPR029063, SAM-dependent_MTases |
Pfami | View protein in Pfam PF07669, Eco57I, 1 hit PF02384, N6_Mtase, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
PROSITEi | View protein in PROSITE PS00092, N6_MTASE, 1 hit |
i Sequence
Sequence statusi: Complete.
P44414-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDESKKISLG QFFTPTHIVK YMIGLMTKNK NASILEPSSG NGVFLDSLIQ
60 70 80 90 100
LGYTNLTSYE IDGDIISHPF VINSSFITSY DKPQYDSIIG NPPYVRWKNL
110 120 130 140 150
SELQKKELKD NSIWKMYCNS LCDYFYIFII KSILQLKVGG ELIFICPDYF
160 170 180 190 200
FSTKNAEGLR KFLINNGSFE KIILFNESKV FHGVSSSVVI FKYIKGKNID
210 220 230 240 250
NINIINIDSK SPIKSEDIES LGESYYIPRF SSSDVWVTSP NHIKVALDKF
260 270 280 290 300
ESYCKTIKKV QPKSLFDDLE FSRIGSVCDI GNGMVSGLDK AFQMNDINYS
310 320 330 340 350
ELELLNSICV AKAKHLDAFC FSGYTRYKFI LDDINEDKLI TYFPNFFYEF
360 370 380 390 400
NNYKDYLLKR YSYNKYLPYW KWAFLRNFSL FSKNEKKIFV PCKERISKKS
410 420 430 440 450
NFRFSLVDEF IYPTQDVTAL YKKENVKESI EYITAYLNSK AVFLWMKYKG
460 470 480 490 500
VVKGNVVEFS EKPLANIPFR RIDWQLKSEK KIHDDITNLV RKYLSNKEFS
510
ILHEINLNLE KLGIKVEI
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L42023 Genomic DNA Translation: AAC22171.1 |
PIRi | F64073 |
RefSeqi | NP_438671.1, NC_000907.1 WP_010868995.1, NC_000907.1 |
Genome annotation databases
EnsemblBacteriai | AAC22171; AAC22171; HI_0513 |
KEGGi | hin:HI0513 |
PATRICi | fig|71421.8.peg.532 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L42023 Genomic DNA Translation: AAC22171.1 |
PIRi | F64073 |
RefSeqi | NP_438671.1, NC_000907.1 WP_010868995.1, NC_000907.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 71421.HI_0513 |
Protein family/group databases
REBASEi | 3427, M.HindII |
Genome annotation databases
EnsemblBacteriai | AAC22171; AAC22171; HI_0513 |
KEGGi | hin:HI0513 |
PATRICi | fig|71421.8.peg.532 |
Phylogenomic databases
eggNOGi | COG0827, Bacteria |
HOGENOMi | CLU_508657_0_0_6 |
Enzyme and pathway databases
BioCyci | HINF71421:G1GJ1-526-MONOMER |
Miscellaneous databases
PROi | PR:P44414 |
Family and domain databases
InterProi | View protein in InterPro IPR003356, DNA_methylase_A-5 IPR002052, DNA_methylase_N6_adenine_CS IPR011639, RM_methylase_Eco57I IPR029063, SAM-dependent_MTases |
Pfami | View protein in Pfam PF07669, Eco57I, 1 hit PF02384, N6_Mtase, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
PROSITEi | View protein in PROSITE PS00092, N6_MTASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MTHD_HAEIN | |
Accessioni | P44414Primary (citable) accession number: P44414 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | November 1, 1995 | |
Last modified: | December 2, 2020 | |
This is version 104 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - Restriction enzymes and methylases
Classification of restriction enzymes and methylases and list of entries