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UniProtKB - P43773 (HSLU_HAEIN)
Protein
ATP-dependent protease ATPase subunit HslU
Gene
hslU
Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Functioni
ATPase subunit of a proteasome-like degradation complex; this subunit has chaperone activity. The binding of ATP and its subsequent hydrolysis by HslU are essential for unfolding of protein substrates subsequently hydrolyzed by HslV. HslU recognizes the N-terminal part of its protein substrates and unfolds these before they are guided to HslV for hydrolysis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 18 | ATP; via amide nitrogen and carbonyl oxygen1 Publication | 1 | |
Binding sitei | 257 | ATPBy similarity | 1 | |
Binding sitei | 322 | ATPBy similarity | 1 | |
Binding sitei | 394 | ATPBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 60 – 65 | ATP1 Publication | 6 | |
Nucleotide bindingi | 306 – 309 | ATP1 Publication | 4 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-UniRule
- ATP hydrolysis activity Source: InterPro
- peptidase activity Source: InterPro
- proteasome-activating activity Source: UniProtKB-UniRule
GO - Biological processi
- protein unfolding Source: UniProtKB-UniRule
Keywordsi
Molecular function | Chaperone |
Ligand | ATP-binding, Nucleotide-binding |
Protein family/group databases
MEROPSi | X20.005 |
Names & Taxonomyi
Protein namesi | Recommended name: ATP-dependent protease ATPase subunit HslUAlternative name(s): Unfoldase HslU |
Gene namesi | Name:hslU Ordered Locus Names:HI_0497 |
Organismi | Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) |
Taxonomic identifieri | 71421 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm By similarity
Cytosol
- HslUV protease complex Source: UniProtKB-UniRule
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000160508 | 1 – 444 | ATP-dependent protease ATPase subunit HslUAdd BLAST | 444 |
Interactioni
Subunit structurei
A double ring-shaped homohexamer of HslV is capped on each side by a ring-shaped HslU homohexamer. The assembly of the HslU/HslV complex is dependent on binding of ATP.
3 PublicationsBinary interactionsi
P43773
With | #Exp. | IntAct |
---|---|---|
hslV [P43772] | 4 | EBI-1030296,EBI-1030290 |
Protein-protein interaction databases
DIPi | DIP-6175N |
IntActi | P43773, 1 interactor |
STRINGi | 71421.HI_0497 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P43773 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P43773 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 143 – 163 | DisorderedSequence analysisAdd BLAST | 21 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 145 – 163 | Basic and acidic residuesSequence analysisAdd BLAST | 19 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG1220, Bacteria |
HOGENOMi | CLU_033123_0_0_6 |
OMAi | KYGMIKT |
PhylomeDBi | P43773 |
Family and domain databases
Gene3Di | 3.40.50.300, 2 hits |
HAMAPi | MF_00249, HslU, 1 hit |
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR003959, ATPase_AAA_core IPR019489, Clp_ATPase_C IPR004491, HslU IPR027417, P-loop_NTPase |
PANTHERi | PTHR48102:SF3, PTHR48102:SF3, 1 hit |
Pfami | View protein in Pfam PF00004, AAA, 1 hit PF07724, AAA_2, 1 hit PF10431, ClpB_D2-small, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 1 hit SM01086, ClpB_D2-small, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00390, hslU, 1 hit |
i Sequence
Sequence statusi: Complete.
P43773-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSEMTPREIV SELDQHIIGQ ADAKRAVAIA LRNRWRRMQL QEPLRHEVTP
60 70 80 90 100
KNILMIGPTG VGKTEIARRL AKLANAPFIK VEATKFTEVG YVGKEVDSII
110 120 130 140 150
RDLTDSAMKL VRQQEIAKNR ARAEDVAEER ILDALLPPAK NQWGEVENHD
160 170 180 190 200
SHSSTRQAFR KKLREGQLDD KEIEIDVSAG VSMGVEIMAP PGMEEMTNQL
210 220 230 240 250
QSLFQNLGSD KTKKRKMKIK DALKALIDDE AAKLINPEEL KQKAIDAVEQ
260 270 280 290 300
NGIVFIDEID KICKKGEYSG ADVSREGVQR DLLPLVEGST VSTKHGMVKT
310 320 330 340 350
DHILFIASGA FQVARPSDLI PELQGRLPIR VELTALSAAD FERILTEPHA
360 370 380 390 400
SLTEQYKALM ATEGVNIAFT TDAVKKIAEA AFRVNEKTEN IGARRLHTVM
410 420 430 440
ERLMDKISFS ASDMNGQTVN IDAAYVADAL GEVVENEDLS RFIL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L42023 Genomic DNA Translation: AAC22154.1 |
RefSeqi | NP_438655.1, NC_000907.1 WP_010868991.1, NC_000907.1 |
Genome annotation databases
EnsemblBacteriai | AAC22154; AAC22154; HI_0497 |
KEGGi | hin:HI_0497 |
PATRICi | fig|71421.8.peg.515 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L42023 Genomic DNA Translation: AAC22154.1 |
RefSeqi | NP_438655.1, NC_000907.1 WP_010868991.1, NC_000907.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1G3I | X-ray | 3.41 | A/B/C/D/E/F/S/T/U/V/W/X | 1-444 | [»] | |
1G41 | X-ray | 2.30 | A | 1-444 | [»] | |
1IM2 | X-ray | 2.80 | A | 1-444 | [»] | |
1KYI | X-ray | 3.10 | A/B/C/D/E/F/S/T/U/V/W/X | 1-444 | [»] | |
1OFH | X-ray | 2.50 | A/B/C | 1-107 | [»] | |
A/B/C | 244-444 | [»] | ||||
1OFI | X-ray | 3.20 | A/B/C | 1-107 | [»] | |
A/B/C | 244-444 | [»] | ||||
SMRi | P43773 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
DIPi | DIP-6175N |
IntActi | P43773, 1 interactor |
STRINGi | 71421.HI_0497 |
Protein family/group databases
MEROPSi | X20.005 |
Genome annotation databases
EnsemblBacteriai | AAC22154; AAC22154; HI_0497 |
KEGGi | hin:HI_0497 |
PATRICi | fig|71421.8.peg.515 |
Phylogenomic databases
eggNOGi | COG1220, Bacteria |
HOGENOMi | CLU_033123_0_0_6 |
OMAi | KYGMIKT |
PhylomeDBi | P43773 |
Miscellaneous databases
EvolutionaryTracei | P43773 |
Family and domain databases
Gene3Di | 3.40.50.300, 2 hits |
HAMAPi | MF_00249, HslU, 1 hit |
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR003959, ATPase_AAA_core IPR019489, Clp_ATPase_C IPR004491, HslU IPR027417, P-loop_NTPase |
PANTHERi | PTHR48102:SF3, PTHR48102:SF3, 1 hit |
Pfami | View protein in Pfam PF00004, AAA, 1 hit PF07724, AAA_2, 1 hit PF10431, ClpB_D2-small, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 1 hit SM01086, ClpB_D2-small, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00390, hslU, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | HSLU_HAEIN | |
Accessioni | P43773Primary (citable) accession number: P43773 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | November 1, 1995 | |
Last modified: | February 23, 2022 | |
This is version 148 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families