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UniProtKB - P43681 (ACHA4_HUMAN)

Entry version 205 (22 Apr 2020)
Sequence version 2 (01 Feb 1996)
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Protein

Neuronal acetylcholine receptor subunit alpha-4

Gene

CHRNA4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane permeable to sodium ions.1 Publication

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Complete GO annotation on QuickGO ...

GO - Biological processi

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionIon channel, Ligand-gated ion channel, Receptor
Biological processIon transport, Transport

Enzyme and pathway databases

Reactome - a knowledgebase of biological pathways and processes

More...Reactomei		R-HSA-629587 Highly sodium permeable acetylcholine nicotinic receptors
R-HSA-629594 Highly calcium permeable postsynaptic nicotinic acetylcholine receptors
R-HSA-629597 Highly calcium permeable nicotinic acetylcholine receptors

Protein family/group databases

Transport Classification Database

More...TCDBi		1.A.9.1.6 the neurotransmitter receptor, cys loop, ligand-gated ion channel (lic) family

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Neuronal acetylcholine receptor subunit alpha-4
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:CHRNA4
Synonyms:NACRA4
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiHomo sapiens (Human)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri9606 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000005640 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 20

Organism-specific databases

Human Gene Nomenclature Database

More...HGNCi		HGNC:1958 CHRNA4

Online Mendelian Inheritance in Man (OMIM)

More...MIMi		118504 gene

neXtProt; the human protein knowledge platform

More...neXtProti		NX_P43681

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular%5Flocation%5Fsection">'Subcellular location'</a> section describes the subcellular compartment where each non-membrane region of a membrane-spanning protein is found.<p><a href='/help/topo_dom' target='_top'>More...</a></p>Topological domaini29 – 242ExtracellularSequence analysisAdd BLAST214
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular%5Flocation%5Fsection">'Subcellular location'</a> section describes the extent of a membrane-spanning region of the protein. It denotes the presence of both alpha-helical transmembrane regions and the membrane spanning regions of beta-barrel transmembrane proteins.<p><a href='/help/transmem' target='_top'>More...</a></p>Transmembranei243 – 267HelicalSequence analysisAdd BLAST25
Transmembranei275 – 293HelicalSequence analysisAdd BLAST19
Transmembranei309 – 330HelicalSequence analysisAdd BLAST22
Topological domaini331 – 600CytoplasmicSequence analysisAdd BLAST270
Transmembranei601 – 619HelicalSequence analysisAdd BLAST19

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This subsection of the 'Pathology and Biotech' section provides information on the disease(s) associated with genetic variations in a given protein. The information is extracted from the scientific literature and diseases that are also described in the <a href="http://www.ncbi.nlm.nih.gov/sites/entrez?db=omim">OMIM</a> database are represented with a <a href="http://www.uniprot.org/diseases">controlled vocabulary</a> in the following way:<p><a href='/help/involvement_in_disease' target='_top'>More...</a></p>Involvement in diseasei

Epilepsy, nocturnal frontal lobe, 1 (ENFL1)3 Publications
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionAn autosomal dominant focal epilepsy characterized by nocturnal seizures with hyperkinetic automatisms and poorly organized stereotyped movements.
Related information in OMIM
Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes natural variant(s) of the protein sequence.<p><a href='/help/variant' target='_top'>More...</a></p>Natural variantiVAR_000295280S → F in ENFL1. 1 PublicationCorresponds to variant dbSNP:rs121909580EnsemblClinVar.1
Natural variantiVAR_017531280S → L in ENFL1. 2 Publications1

Keywords - Diseasei

Disease mutation, Epilepsy

Organism-specific databases

DisGeNET

More...DisGeNETi		1137

GeneReviews a resource of expert-authored, peer-reviewed disease descriptions.

More...GeneReviewsi		CHRNA4

MalaCards human disease database

More...MalaCardsi		CHRNA4
MIMi600513 phenotype

Open Targets

More...OpenTargetsi		ENSG00000101204

Orphanet; a database dedicated to information on rare diseases and orphan drugs

More...Orphaneti		98784 Autosomal dominant nocturnal frontal lobe epilepsy

The Pharmacogenetics and Pharmacogenomics Knowledge Base

More...PharmGKBi		PA26490

Miscellaneous databases

Pharos NIH Druggable Genome Knowledgebase

More...Pharosi		P43681 Tclin

Chemistry databases

ChEMBL database of bioactive drug-like small molecules

More...ChEMBLi		CHEMBL1882

Drug and drug target database

More...DrugBanki		DB00915 Amantadine
DB01351 Amobarbital
DB01352 Aprobarbital
DB00572 Atropine
DB01483 Barbital
DB01496 Barbituric acid derivative
DB00237 Butabarbital
DB00241 Butalbital
DB01353 Butobarbital
DB00564 Carbamazepine
DB09028 Cytisine
DB01245 Decamethonium
DB00514 Dextromethorphan
DB07720 Epibatidine
DB00783 Estradiol
DB13952 Estradiol acetate
DB13953 Estradiol benzoate
DB13954 Estradiol cypionate
DB13955 Estradiol dienanthate
DB13956 Estradiol valerate
DB00898 Ethanol
DB00674 Galantamine
DB01354 Heptabarbital
DB01355 Hexobarbital
DB00753 Isoflurane
DB00657 Mecamylamine
DB00333 Methadone
DB00463 Metharbital
DB00849 Methylphenobarbital
DB00184 Nicotine
DB00312 Pentobarbital
DB01174 Phenobarbital
DB00981 Physostigmine
DB05458 Pozanicline
DB00794 Primidone
DB05740 RPI-78M
DB00747 Scopolamine
DB00418 Secobarbital
DB00306 Talbutal
DB00599 Thiopental
DB01273 Varenicline

DrugCentral

More...DrugCentrali		P43681

IUPHAR/BPS Guide to PHARMACOLOGY

More...GuidetoPHARMACOLOGYi		465

Polymorphism and mutation databases

BioMuta curated single-nucleotide variation and disease association database

More...BioMutai		CHRNA4

Domain mapping of disease mutations (DMDM)

More...DMDMi		1351848

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section denotes the presence of an N-terminal signal peptide.<p><a href='/help/signal' target='_top'>More...</a></p>Signal peptidei1 – 28Sequence analysisAdd BLAST28
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_000000035129 – 627Neuronal acetylcholine receptor subunit alpha-4Add BLAST599

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM / Processing</a> section specifies the position and type of each covalently attached glycan group (mono-, di-, or polysaccharide).<p><a href='/help/carbohyd' target='_top'>More...</a></p>Glycosylationi57N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi107N-linked (GlcNAc...) asparagineSequence analysis1
<p>This subsection of the PTM / Processing":/help/ptm_processing_section section describes the positions of cysteine residues participating in disulfide bonds.<p><a href='/help/disulfid' target='_top'>More...</a></p>Disulfide bondi161 ↔ 175By similarity
Glycosylationi174N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi225 ↔ 226Associated with receptor activationBy similarity
<p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM / Processing</a> section specifies the position(s) and the type of covalently attached lipid group(s).<p><a href='/help/lipid' target='_top'>More...</a></p>Lipidationi271S-palmitoyl cysteineBy similarity1
<p>This subsection of the 'PTM / Processing' section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei424PhosphoserineBy similarity1
Modified residuei538PhosphoserineBy similarity1
Modified residuei541PhosphoserineBy similarity1

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

MassIVE - Mass Spectrometry Interactive Virtual Environment

More...MassIVEi		P43681

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		P43681

PeptideAtlas

More...PeptideAtlasi		P43681

PRoteomics IDEntifications database

More...PRIDEi		P43681

ProteomicsDB: a multi-organism proteome resource

More...ProteomicsDBi		55649 [P43681-1]
62290

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...iPTMneti		P43681

Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

More...PhosphoSitePlusi		P43681

SwissPalm database of S-palmitoylation events

More...SwissPalmi		P43681

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Bgee dataBase for Gene Expression Evolution

More...Bgeei		ENSG00000101204 Expressed in right lobe of liver and 76 other tissues

ExpressionAtlas, Differential and Baseline Expression

More...ExpressionAtlasi		P43681 baseline and differential

Genevisible search portal to normalized and curated expression data from Genevestigator

More...Genevisiblei		P43681 HS

Organism-specific databases

Human Protein Atlas

More...HPAi		ENSG00000101204 Group enriched (brain, liver, parathyroid gland)

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Neuronal AChR is composed of two different types of subunits: alpha and beta. Alpha-4 subunit can be combined to beta-2 or beta-4 to give rise to functional receptors, complexes with beta-2 may be heteropentamers (PubMed:22361591).

Interacts with RIC3; which is required for proper folding and assembly (PubMed:16120769).

Interacts with LYPD6 (PubMed:27344019). The heteropentamer alpha-4-beta-2 interacts with alpha-conotoxins PnIA, GID and MII (By similarity).

By similarity3 Publications

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction%5Fsection%27">Interaction</a> section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="https://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated at every <a href="http://www.uniprot.org/help/synchronization">UniProt release</a>.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

Show more details

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGrid)

More...BioGridi		107559, 4 interactors

ComplexPortal: manually curated resource of macromolecular complexes

More...ComplexPortali		CPX-168 Neuronal nicotinic acetylcholine receptor complex, 3xalpha4-2xbeta2
CPX-218 Neuronal nicotinic acetylcholine receptor complex, alpha4-alpha5-beta2
CPX-2180 Neuronal nicotinic acetylcholine receptor complex, 2xalpha4-3xbeta2
CPX-2203 Neuronal nicotinic acetylcholine receptor complex, alpha4-beta4

CORUM comprehensive resource of mammalian protein complexes

More...CORUMi		P43681

Protein interaction database and analysis system

More...IntActi		P43681, 6 interactors

Molecular INTeraction database

More...MINTi		P43681

STRING: functional protein association networks

More...STRINGi		9606.ENSP00000359285

Chemistry databases

BindingDB database of measured binding affinities

More...BindingDBi		P43681

Miscellaneous databases

RNAct, Protein-RNA interaction predictions for model organisms.

More...RNActi		P43681 protein

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

1627
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		P43681

Database of comparative protein structure models

More...ModBasei		Search...

Protein Data Bank in Europe - Knowledge Base

More...PDBe-KBi		Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-4/CHRNA4 sub-subfamily. [View classification]Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		KOG3645 Eukaryota
ENOG410XQGR LUCA

Ensembl GeneTree

More...GeneTreei		ENSGT00940000159329

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...HOGENOMi		CLU_018074_1_1_1

InParanoid: Eukaryotic Ortholog Groups

More...InParanoidi		P43681

KEGG Orthology (KO)

More...KOi		K04806

Identification of Orthologs from Complete Genome Data

More...OMAi		VQQMYSP

Database for complete collections of gene phylogenies

More...PhylomeDBi		P43681

TreeFam database of animal gene trees

More...TreeFami		TF315605

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...Gene3Di		2.70.170.10, 1 hit

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR006202 Neur_chan_lig-bd
IPR036734 Neur_chan_lig-bd_sf
IPR006201 Neur_channel
IPR036719 Neuro-gated_channel_TM_sf
IPR006029 Neurotrans-gated_channel_TM
IPR018000 Neurotransmitter_ion_chnl_CS
IPR002394 Nicotinic_acetylcholine_rcpt

The PANTHER Classification System

More...PANTHERi		PTHR18945 PTHR18945, 1 hit

Pfam protein domain database

More...Pfami		View protein in Pfam
PF02931 Neur_chan_LBD, 1 hit
PF02932 Neur_chan_memb, 1 hit

Protein Motif fingerprint database; a protein domain database

More...PRINTSi		PR00254 NICOTINICR
PR00252 NRIONCHANNEL

Superfamily database of structural and functional annotation

More...SUPFAMi		SSF63712 SSF63712, 1 hit
SSF90112 SSF90112, 1 hit

TIGRFAMs; a protein family database

More...TIGRFAMsi		TIGR00860 LIC, 1 hit

PROSITE; a protein domain and family database

More...PROSITEi		View protein in PROSITE
PS00236 NEUROTR_ION_CHANNEL, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (2+)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is in its mature form or if it represents the precursor.<p><a href='/help/sequence_processing' target='_top'>More...</a></p>Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 <p>This subsection of the 'Sequence' section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform. This section is only present in reviewed entries, i.e. in UniProtKB/Swiss-Prot.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: P43681-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the <div> <p><b>What is the canonical sequence?</b><p><a href='/help/canonical_and_isoforms' target='_top'>More...</a></p>canonicali sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MELGGPGAPR LLPPLLLLLG TGLLRASSHV ETRAHAEERL LKKLFSGYNK 
        60         70         80         90        100
WSRPVANISD VVLVRFGLSI AQLIDVDEKN QMMTTNVWVK QEWHDYKLRW 
       110        120        130        140        150
DPADYENVTS IRIPSELIWR PDIVLYNNAD GDFAVTHLTK AHLFHDGRVQ 
       160        170        180        190        200
WTPPAIYKSS CSIDVTFFPF DQQNCTMKFG SWTYDKAKID LVNMHSRVDQ 
       210        220        230        240        250
LDFWESGEWV IVDAVGTYNT RKYECCAEIY PDITYAFVIR RLPLFYTINL 
       260        270        280        290        300
IIPCLLISCL TVLVFYLPSE CGEKITLCIS VLLSLTVFLL LITEIIPSTS 
       310        320        330        340        350
LVIPLIGEYL LFTMIFVTLS IVITVFVLNV HHRSPRTHTM PTWVRRVFLD 
       360        370        380        390        400
IVPRLLLMKR PSVVKDNCRR LIESMHKMAS APRFWPEPEG EPPATSGTQS 
       410        420        430        440        450
LHPPSPSFCV PLDVPAEPGP SCKSPSDQLP PQQPLEAEKA SPHPSPGPCR 
       460        470        480        490        500
PPHGTQAPGL AKARSLSVQH MSSPGEAVEG GVRCRSRSIQ YCVPRDDAAP 
       510        520        530        540        550
EADGQAAGAL ASRNTHSAEL PPPDQPSPCK CTCKKEPSSV SPSATVKTRS 
       560        570        580        590        600
TKAPPPHLPL SPALTRAVEG VQYIADHLKA EDTDFSVKED WKYVAMVIDR 
       610        620 
IFLWMFIIVC LLGTVGLFLP PWLAGMI
Length:627
Mass (Da):69,957
Last modified:February 1, 1996 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iB3A0C0151E5A2AA8
GO
Isoform 2 (identifier: P43681-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-57: MELGGPGAPR...YNKWSRPVAN → MRMSPPSASP...VWTAGCPGEG
     58-128: Missing.

Show »
Length:556
Mass (Da):60,994
Checksum:iCE5902DBC1231CA3
GO

<p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0D9SFU6A0A0D9SFU6_HUMAN
Neuronal acetylcholine receptor sub...
CHRNA4
111Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
H0YBH1H0YBH1_HUMAN
Neuronal acetylcholine receptor sub...
CHRNA4
137Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti4G → E in AAH96290 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_000295280S → F in ENFL1. 1 PublicationCorresponds to variant dbSNP:rs121909580EnsemblClinVar.1
Natural variantiVAR_017531280S → L in ENFL1. 2 Publications1
Natural variantiVAR_023402387E → G1 PublicationCorresponds to variant dbSNP:rs45604738EnsemblClinVar.1
Natural variantiVAR_023403517S → L1 PublicationCorresponds to variant dbSNP:rs45622132EnsemblClinVar.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes the sequence of naturally occurring alternative protein isoform(s). The changes in the amino acid sequence may be due to alternative splicing, alternative promoter usage, alternative initiation, or ribosomal frameshifting.<p><a href='/help/var_seq' target='_top'>More...</a></p>Alternative sequenceiVSP_0542751 – 57MELGG…RPVAN → MRMSPPSASPPSSSGGRTSS STTTAGEPFWAGVLFAIRPH PGLSGRIVWTAGCPGEG in isoform 2. 1 PublicationAdd BLAST57
Alternative sequenceiVSP_05427658 – 128Missing in isoform 2. 1 PublicationAdd BLAST71

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
L35901 mRNA Translation: AAA64743.1
X89741 X89746 Genomic DNA Translation: CAA61893.1
U62433 mRNA Translation: AAB40111.1
Y08421 mRNA Translation: CAA69698.1
DQ093071 Genomic DNA Translation: AAY88737.1
AL121827 Genomic DNA No translation available.
BC096290 mRNA Translation: AAH96290.1
BC096291 mRNA Translation: AAH96291.1
X87629 mRNA Translation: CAA60959.1

The Consensus CDS (CCDS) project

More...CCDSi		CCDS13517.1 [P43681-1]

Protein sequence database of the Protein Information Resource

More...PIRi		JC4021

NCBI Reference Sequences

More...RefSeqi		NP_000735.1, NM_000744.6 [P43681-1]
NP_001243502.1, NM_001256573.1
XP_011526826.1, XM_011528524.1 [P43681-2]

Genome annotation databases

Ensembl eukaryotic genome annotation project

More...Ensembli		ENST00000370263; ENSP00000359285; ENSG00000101204 [P43681-1]
ENST00000615287; ENSP00000483388; ENSG00000101204 [P43681-2]

Database of genes from NCBI RefSeq genomes

More...GeneIDi		1137

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		hsa:1137

UCSC genome browser

More...UCSCi		uc002yes.4 human [P43681-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

<p>This subsection of the <a href="http://www.uniprot.org/manual/cross%5Freferences%5Fsection">Cross-references</a> section provides links to various web resources that are relevant for a specific protein.<p><a href='/help/web_resource' target='_top'>More...</a></p>Web resourcesi

NIEHS-SNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L35901 mRNA Translation: AAA64743.1
X89741 X89746 Genomic DNA Translation: CAA61893.1
U62433 mRNA Translation: AAB40111.1
Y08421 mRNA Translation: CAA69698.1
DQ093071 Genomic DNA Translation: AAY88737.1
AL121827 Genomic DNA No translation available.
BC096290 mRNA Translation: AAH96290.1
BC096291 mRNA Translation: AAH96291.1
X87629 mRNA Translation: CAA60959.1
CCDSiCCDS13517.1 [P43681-1]
PIRiJC4021
RefSeqiNP_000735.1, NM_000744.6 [P43681-1]
NP_001243502.1, NM_001256573.1
XP_011526826.1, XM_011528524.1 [P43681-2]

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...PDBei

Protein Data Bank RCSB

More...RCSB PDBi

Protein Data Bank Japan

More...PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2GVTmodel-A/C38-241[»]
2LLYNMR-A240-339[»]
A598-626[»]
5KXIX-ray3.94A/D27-364[»]
A/D586-627[»]
6CNJelectron microscopy3.40A/D27-364[»]
6CNKelectron microscopy3.70A/B/D27-364[»]
SMRiP43681
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

BioGridi107559, 4 interactors
ComplexPortaliCPX-168 Neuronal nicotinic acetylcholine receptor complex, 3xalpha4-2xbeta2
CPX-218 Neuronal nicotinic acetylcholine receptor complex, alpha4-alpha5-beta2
CPX-2180 Neuronal nicotinic acetylcholine receptor complex, 2xalpha4-3xbeta2
CPX-2203 Neuronal nicotinic acetylcholine receptor complex, alpha4-beta4
CORUMiP43681
IntActiP43681, 6 interactors
MINTiP43681
STRINGi9606.ENSP00000359285

Chemistry databases

BindingDBiP43681
ChEMBLiCHEMBL1882
DrugBankiDB00915 Amantadine
DB01351 Amobarbital
DB01352 Aprobarbital
DB00572 Atropine
DB01483 Barbital
DB01496 Barbituric acid derivative
DB00237 Butabarbital
DB00241 Butalbital
DB01353 Butobarbital
DB00564 Carbamazepine
DB09028 Cytisine
DB01245 Decamethonium
DB00514 Dextromethorphan
DB07720 Epibatidine
DB00783 Estradiol
DB13952 Estradiol acetate
DB13953 Estradiol benzoate
DB13954 Estradiol cypionate
DB13955 Estradiol dienanthate
DB13956 Estradiol valerate
DB00898 Ethanol
DB00674 Galantamine
DB01354 Heptabarbital
DB01355 Hexobarbital
DB00753 Isoflurane
DB00657 Mecamylamine
DB00333 Methadone
DB00463 Metharbital
DB00849 Methylphenobarbital
DB00184 Nicotine
DB00312 Pentobarbital
DB01174 Phenobarbital
DB00981 Physostigmine
DB05458 Pozanicline
DB00794 Primidone
DB05740 RPI-78M
DB00747 Scopolamine
DB00418 Secobarbital
DB00306 Talbutal
DB00599 Thiopental
DB01273 Varenicline
DrugCentraliP43681
GuidetoPHARMACOLOGYi465

Protein family/group databases

TCDBi1.A.9.1.6 the neurotransmitter receptor, cys loop, ligand-gated ion channel (lic) family

PTM databases

iPTMnetiP43681
PhosphoSitePlusiP43681
SwissPalmiP43681

Polymorphism and mutation databases

BioMutaiCHRNA4
DMDMi1351848

Proteomic databases

MassIVEiP43681
PaxDbiP43681
PeptideAtlasiP43681
PRIDEiP43681
ProteomicsDBi55649 [P43681-1]
62290

Protocols and materials databases

Antibodypedia a portal for validated antibodies

More...Antibodypediai		29717 269 antibodies

Genome annotation databases

EnsembliENST00000370263; ENSP00000359285; ENSG00000101204 [P43681-1]
ENST00000615287; ENSP00000483388; ENSG00000101204 [P43681-2]
GeneIDi1137
KEGGihsa:1137
UCSCiuc002yes.4 human [P43681-1]

Organism-specific databases

Comparative Toxicogenomics Database

More...CTDi		1137
DisGeNETi1137

GeneCards: human genes, protein and diseases

More...GeneCardsi		CHRNA4
GeneReviewsiCHRNA4
HGNCiHGNC:1958 CHRNA4
HPAiENSG00000101204 Group enriched (brain, liver, parathyroid gland)
MalaCardsiCHRNA4
MIMi118504 gene
600513 phenotype
neXtProtiNX_P43681
OpenTargetsiENSG00000101204
Orphaneti98784 Autosomal dominant nocturnal frontal lobe epilepsy
PharmGKBiPA26490

GenAtlas: human gene database

More...GenAtlasi		Search...

Phylogenomic databases

eggNOGiKOG3645 Eukaryota
ENOG410XQGR LUCA
GeneTreeiENSGT00940000159329
HOGENOMiCLU_018074_1_1_1
InParanoidiP43681
KOiK04806
OMAiVQQMYSP
PhylomeDBiP43681
TreeFamiTF315605

Enzyme and pathway databases

ReactomeiR-HSA-629587 Highly sodium permeable acetylcholine nicotinic receptors
R-HSA-629594 Highly calcium permeable postsynaptic nicotinic acetylcholine receptors
R-HSA-629597 Highly calcium permeable nicotinic acetylcholine receptors

Miscellaneous databases

The Gene Wiki collection of pages on human genes and proteins

More...GeneWikii		CHRNA4

Database of phenotypes from RNA interference screens in Drosophila and Homo sapiens

More...GenomeRNAii		1137
PharosiP43681 Tclin

Protein Ontology

More...PROi		PR:P43681
RNActiP43681 protein

The Stanford Online Universal Resource for Clones and ESTs

More...SOURCEi		Search...

Gene expression databases

BgeeiENSG00000101204 Expressed in right lobe of liver and 76 other tissues
ExpressionAtlasiP43681 baseline and differential
GenevisibleiP43681 HS

Family and domain databases

Gene3Di2.70.170.10, 1 hit
InterProiView protein in InterPro
IPR006202 Neur_chan_lig-bd
IPR036734 Neur_chan_lig-bd_sf
IPR006201 Neur_channel
IPR036719 Neuro-gated_channel_TM_sf
IPR006029 Neurotrans-gated_channel_TM
IPR018000 Neurotransmitter_ion_chnl_CS
IPR002394 Nicotinic_acetylcholine_rcpt
PANTHERiPTHR18945 PTHR18945, 1 hit
PfamiView protein in Pfam
PF02931 Neur_chan_LBD, 1 hit
PF02932 Neur_chan_memb, 1 hit
PRINTSiPR00254 NICOTINICR
PR00252 NRIONCHANNEL
SUPFAMiSSF63712 SSF63712, 1 hit
SSF90112 SSF90112, 1 hit
TIGRFAMsiTIGR00860 LIC, 1 hit
PROSITEiView protein in PROSITE
PS00236 NEUROTR_ION_CHANNEL, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...ProtoNeti		Search...

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiACHA4_HUMAN
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P43681
Secondary accession number(s): Q4JGR7, Q4VAQ5, Q4VAQ6
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: February 1, 1996
Last modified: April 22, 2020
This is version 205 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human chromosome 20
    Human chromosome 20: entries, gene names and cross-references to MIM
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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