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Entry version 169 (02 Dec 2020)
Sequence version 1 (01 Nov 1995)
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Protein

Citrate synthase 3, mitochondrial

Gene

CIT3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Dual specificity mitochondrial citrate and methylcitrate synthase with similar catalytic efficiency with both acetyl-CoA and propionyl-CoA.2 Publications

Miscellaneous

Citrate synthase is found in nearly all cells capable of oxidative metabolism.Curated

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the 'Function' section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

  1. KM=1200 µM for acetyl-CoA1 Publication
  2. KM=520 µM for propionyl-CoA1 Publication

    <p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: tricarboxylic acid cycle

    This protein is involved in step 1 of the subpathway that synthesizes isocitrate from oxaloacetate.Curated
    Proteins known to be involved in the 2 steps of the subpathway in this organism are:
    1. Citrate synthase, mitochondrial (CIT1), Citrate synthase 3, mitochondrial (CIT3), Citrate synthase, peroxisomal (CIT2)
    2. Aconitate hydratase, mitochondrial (ACO1)
    This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes isocitrate from oxaloacetate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei315PROSITE-ProRule annotation1
    Active sitei361PROSITE-ProRule annotation1
    Active sitei419PROSITE-ProRule annotation1

    <p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

    GO - Biological processi

    <p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

    Molecular functionTransferase
    Biological processTricarboxylic acid cycle

    Enzyme and pathway databases

    BioCyc Collection of Pathway/Genome Databases

    More...
    BioCyci
    MetaCyc:YPR001W-MONOMER

    UniPathway: a resource for the exploration and annotation of metabolic pathways

    More...
    UniPathwayi
    UPA00223;UER00717

    <p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
    Recommended name:
    Citrate synthase 3, mitochondrial1 Publication (EC:2.3.3.11 Publication)
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
    Name:CIT31 Publication
    Ordered Locus Names:YPR001W
    ORF Names:YP9723.01
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri559292 [NCBI]
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
    • UP000002311 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome XVI

    Organism-specific databases

    Eukaryotic Pathogen and Host Database Resources

    More...
    EuPathDBi
    FungiDB:YPR001W

    Saccharomyces Genome Database

    More...
    SGDi
    S000006205, CIT3

    <p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Mitochondrion

    <p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

    <p>This subsection of the 'Pathology and Biotech' section describes the in vivo effects caused by ablation of the gene (or one or more transcripts) coding for the protein described in the entry. This includes gene knockout and knockdown, provided experiments have been performed in the context of a whole organism or a specific tissue, and not at the single-cell level.<p><a href='/help/disruption_phenotype' target='_top'>More...</a></p>Disruption phenotypei

    Leads to an accumulation of acetate and of isobutanol.1 Publication

    <p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the 'PTM / Processing' section describes the extent of a transit peptide.<p><a href='/help/transit' target='_top'>More...</a></p>Transit peptidei1 – 23MitochondrionSequence analysisAdd BLAST23
    <p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_000016998524 – 486Citrate synthase 3, mitochondrialAdd BLAST463

    Proteomic databases

    PaxDb, a database of protein abundance averages across all three domains of life

    More...
    PaxDbi
    P43635

    PRoteomics IDEntifications database

    More...
    PRIDEi
    P43635

    <p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

    Protein-protein interaction databases

    The Biological General Repository for Interaction Datasets (BioGRID)

    More...
    BioGRIDi
    36177, 76 interactors

    Database of interacting proteins

    More...
    DIPi
    DIP-3915N

    STRING: functional protein association networks

    More...
    STRINGi
    4932.YPR001W

    Miscellaneous databases

    RNAct, Protein-RNA interaction predictions for model organisms.

    More...
    RNActi
    P43635, protein

    <p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

    3D structure databases

    SWISS-MODEL Repository - a database of annotated 3D protein structure models

    More...
    SMRi
    P43635

    Database of comparative protein structure models

    More...
    ModBasei
    Search...

    <p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

    Motif

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the 'Family and Domains' section describes a short (usually not more than 20 amino acids) conserved sequence motif of biological significance.<p><a href='/help/motif' target='_top'>More...</a></p>Motifi484 – 486Microbody targeting signalSequence analysis3

    <p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

    Belongs to the citrate synthase family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    evolutionary genealogy of genes: Non-supervised Orthologous Groups

    More...
    eggNOGi
    KOG2617, Eukaryota

    Ensembl GeneTree

    More...
    GeneTreei
    ENSGT00390000006813

    The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

    More...
    HOGENOMi
    CLU_022049_2_1_1

    InParanoid: Eukaryotic Ortholog Groups

    More...
    InParanoidi
    P43635

    Identification of Orthologs from Complete Genome Data

    More...
    OMAi
    ANAARCY

    Family and domain databases

    Gene3D Structural and Functional Annotation of Protein Families

    More...
    Gene3Di
    1.10.230.10, 1 hit
    1.10.580.10, 1 hit

    Integrated resource of protein families, domains and functional sites

    More...
    InterProi
    View protein in InterPro
    IPR016142, Citrate_synth-like_lrg_a-sub
    IPR016143, Citrate_synth-like_sm_a-sub
    IPR002020, Citrate_synthase
    IPR019810, Citrate_synthase_AS
    IPR036969, Citrate_synthase_sf

    The PANTHER Classification System

    More...
    PANTHERi
    PTHR11739, PTHR11739, 1 hit

    Pfam protein domain database

    More...
    Pfami
    View protein in Pfam
    PF00285, Citrate_synt, 1 hit

    Protein Motif fingerprint database; a protein domain database

    More...
    PRINTSi
    PR00143, CITRTSNTHASE

    Superfamily database of structural and functional annotation

    More...
    SUPFAMi
    SSF48256, SSF48256, 1 hit

    PROSITE; a protein domain and family database

    More...
    PROSITEi
    View protein in PROSITE
    PS00480, CITRATE_SYNTHASE, 1 hit

    <p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

    <p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

    <p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is in its mature form or if it represents the precursor.<p><a href='/help/sequence_processing' target='_top'>More...</a></p>Sequence processingi: The displayed sequence is further processed into a mature form.

    P43635-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MVQRLLPGAH ICRRSFNSSA IIKSSALTLK EALENVIPKK RDAVKKLKAC
    60 70 80 90 100
    YGSTFVGPIT ISSVLGGMRG NQSMFWQGTS LDPEHGIKFQ GLTIEECQNR
    110 120 130 140 150
    LPNTGIDGDN FLPESMLWLL MTGGVPTFQQ AASFRKELAI RGRKLPHYTE
    160 170 180 190 200
    KVLSSLPKDM HPMTQLAIGL ASMNKGSLFA TNYQKGLIGK MEFWKDTLED
    210 220 230 240 250
    SLNLIASLPL LTGRIYSNIT NEGHPLGQYS EEVDWCTNIC SLLGMTNGTN
    260 270 280 290 300
    SSNTCNLTSQ QSLDFINLMR LYTGIHVDHE GGNVSAHTTH LVGSALSDPY
    310 320 330 340 350
    LSYSSGIMGL AGPLHGLAAQ EVVRFLIEMN SNISSIAREQ EIKDYLWKIL
    360 370 380 390 400
    NSNRVIPGYG HAVLRKPDPR FTAMLEFAQK RPIEFENDKN VLLMQKLAEI
    410 420 430 440 450
    APKVLLEHGK SKNPFPNVDS ASGILFYHYG IRELLFFTVI FGCSRAMGPL
    460 470 480
    TQLVWDRILG LPIERPKSLN LEGLEALTKA SNVNKL
    Length:486
    Mass (Da):53,811
    Last modified:November 1, 1995 - v1
    <p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iF299CA258B772125
    GO

    Sequence databases

    Select the link destinations:

    EMBL nucleotide sequence database

    More...
    EMBLi

    GenBank nucleotide sequence database

    More...
    GenBanki

    DNA Data Bank of Japan; a nucleotide sequence database

    More...
    DDBJi
    Links Updated
    X88846 Genomic DNA Translation: CAA61299.1
    Z48951 Genomic DNA Translation: CAA88779.1
    Z71255 Genomic DNA Translation: CAA95041.1
    U31900 Genomic DNA Translation: AAA97580.1
    BK006949 Genomic DNA Translation: DAA11428.1

    Protein sequence database of the Protein Information Resource

    More...
    PIRi
    S52814

    NCBI Reference Sequences

    More...
    RefSeqi
    NP_015325.1, NM_001184098.1

    Genome annotation databases

    Ensembl fungal genome annotation project

    More...
    EnsemblFungii
    YPR001W_mRNA; YPR001W; YPR001W

    Database of genes from NCBI RefSeq genomes

    More...
    GeneIDi
    856107

    KEGG: Kyoto Encyclopedia of Genes and Genomes

    More...
    KEGGi
    sce:YPR001W

    <p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

    <p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X88846 Genomic DNA Translation: CAA61299.1
    Z48951 Genomic DNA Translation: CAA88779.1
    Z71255 Genomic DNA Translation: CAA95041.1
    U31900 Genomic DNA Translation: AAA97580.1
    BK006949 Genomic DNA Translation: DAA11428.1
    PIRiS52814
    RefSeqiNP_015325.1, NM_001184098.1

    3D structure databases

    SMRiP43635
    ModBaseiSearch...

    Protein-protein interaction databases

    BioGRIDi36177, 76 interactors
    DIPiDIP-3915N
    STRINGi4932.YPR001W

    Proteomic databases

    PaxDbiP43635
    PRIDEiP43635

    Genome annotation databases

    EnsemblFungiiYPR001W_mRNA; YPR001W; YPR001W
    GeneIDi856107
    KEGGisce:YPR001W

    Organism-specific databases

    EuPathDBiFungiDB:YPR001W
    SGDiS000006205, CIT3

    Phylogenomic databases

    eggNOGiKOG2617, Eukaryota
    GeneTreeiENSGT00390000006813
    HOGENOMiCLU_022049_2_1_1
    InParanoidiP43635
    OMAiANAARCY

    Enzyme and pathway databases

    UniPathwayiUPA00223;UER00717
    BioCyciMetaCyc:YPR001W-MONOMER

    Miscellaneous databases

    Protein Ontology

    More...
    PROi
    PR:P43635
    RNActiP43635, protein

    Family and domain databases

    Gene3Di1.10.230.10, 1 hit
    1.10.580.10, 1 hit
    InterProiView protein in InterPro
    IPR016142, Citrate_synth-like_lrg_a-sub
    IPR016143, Citrate_synth-like_sm_a-sub
    IPR002020, Citrate_synthase
    IPR019810, Citrate_synthase_AS
    IPR036969, Citrate_synthase_sf
    PANTHERiPTHR11739, PTHR11739, 1 hit
    PfamiView protein in Pfam
    PF00285, Citrate_synt, 1 hit
    PRINTSiPR00143, CITRTSNTHASE
    SUPFAMiSSF48256, SSF48256, 1 hit
    PROSITEiView protein in PROSITE
    PS00480, CITRATE_SYNTHASE, 1 hit

    ProtoNet; Automatic hierarchical classification of proteins

    More...
    ProtoNeti
    Search...

    MobiDB: a database of protein disorder and mobility annotations

    More...
    MobiDBi
    Search...

    <p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

    <p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiCISY3_YEAST
    <p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P43635
    Secondary accession number(s): D6W412
    <p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: December 2, 2020
    This is version 169 of the entry and version 1 of the sequence. See complete history.
    <p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    <p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

    Keywords - Technical termi

    Reference proteome

    Documents

    1. Yeast chromosome XVI
      Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families
    4. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
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