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Protein

Cys-Gly metallodipeptidase DUG1

Gene

DUG1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic component of the GSH degradosomal complex involved in the degradation of glutathione (GSH) and other peptides containing a gamma-glu-X bond. Functions also in a DUG2-DUG3-independent manner as a dipeptidase with high specificity for Cys-Gly and no activity toward tri- or tetrapeptides.2 Publications

Miscellaneous

Present with 22300 molecules/cell in log phase SD medium.1 Publication

Cofactori

Zn2+1 Publication, Mn2+1 Publication

Kineticsi

  1. KM=0.4 mM for Cys-Gly (at pH 8.0 and in the presence of 20 µM manganese ions)
  2. KM=0.8 mM for Cys-Gly (at pH 8.0 and in the presence of 200 µM zinc ions)

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi102Zinc 2By similarity1
    Active sitei104By similarity1
    Metal bindingi137Zinc 1By similarity1
    Metal bindingi137Zinc 2By similarity1
    Active sitei171Proton acceptorBy similarity1
    Metal bindingi172Zinc 1By similarity1
    Metal bindingi200Zinc 2By similarity1
    Metal bindingi450Zinc 1By similarity1

    GO - Molecular functioni

    • metal ion binding Source: UniProtKB-KW
    • metallodipeptidase activity Source: SGD
    • omega peptidase activity Source: SGD

    GO - Biological processi

    • glutathione catabolic process Source: SGD

    Keywordsi

    Molecular functionDipeptidase, Hydrolase, Metalloprotease, Protease
    LigandManganese, Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER3O-4
    YEAST:MONOMER3O-4
    ReactomeiR-SCE-174403 Glutathione synthesis and recycling
    SABIO-RKiP43616

    Protein family/group databases

    MEROPSiM20.017

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cys-Gly metallodipeptidase DUG1 (EC:3.4.13.-)
    Alternative name(s):
    Deficient in utilization of glutathione protein 1
    GSH degradosomal complex subunit DUG1
    Gene namesi
    Name:DUG1
    Ordered Locus Names:YFR044C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    Proteomesi
    • UP000002311 Componenti: Chromosome VI

    Organism-specific databases

    EuPathDBiFungiDB:YFR044C
    SGDiS000001940 DUG1

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Cytoplasm, Mitochondrion

    Pathology & Biotechi

    Disruption phenotypei

    Accumulation of Cys-Gly dipeptide and enhanced GSH toxicity.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001852751 – 481Cys-Gly metallodipeptidase DUG1Add BLAST481

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei451PhosphoserineCombined sources1

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP43616
    PaxDbiP43616
    PRIDEiP43616

    PTM databases

    iPTMnetiP43616

    Interactioni

    Subunit structurei

    Homodimer. Component of the GSH degradosomal complex composed of at least DUG1, DUG2 and DUG3.2 Publications

    Protein-protein interaction databases

    BioGridi31202, 50 interactors
    DIPiDIP-6492N
    IntActiP43616, 4 interactors
    MINTiP43616
    STRINGi4932.YFR044C

    Structurei

    Secondary structure

    1481
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details

    3D structure databases

    ProteinModelPortaliP43616
    SMRiP43616
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase M20A family.Curated

    Phylogenomic databases

    GeneTreeiENSGT00390000009682
    HOGENOMiHOG000216709
    InParanoidiP43616
    KOiK15428
    OMAiHITIPGF
    OrthoDBiEOG092C1SDA

    Family and domain databases

    CDDicd05676 M20_dipept_like_CNDP, 1 hit
    InterProiView protein in InterPro
    IPR017153 CNDP/DUG1
    IPR002933 Peptidase_M20
    IPR011650 Peptidase_M20_dimer
    PfamiView protein in Pfam
    PF07687 M20_dimer, 1 hit
    PF01546 Peptidase_M20, 1 hit
    PIRSFiPIRSF037242 CNDP_dipeptidase, 1 hit

    Sequencei

    Sequence statusi: Complete.

    P43616-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MSHSLTSVFQ KIDSLKPQFF SRLTKAIQIP AVSSDESLRS KVFDKAKFIS
    60 70 80 90 100
    EQLSQSGFHD IKMVDLGIQP PPISTPNLSL PPVILSRFGS DPSKKTVLVY
    110 120 130 140 150
    GHYDVQPAQL EDGWDTEPFK LVIDEAKGIM KGRGVTDDTG PLLSWINVVD
    160 170 180 190 200
    AFKASGQEFP VNLVTCFEGM EESGSLKLDE LIKKEANGYF KGVDAVCISD
    210 220 230 240 250
    NYWLGTKKPV LTYGLRGCNY YQTIIEGPSA DLHSGIFGGV VAEPMIDLMQ
    260 270 280 290 300
    VLGSLVDSKG KILIDGIDEM VAPLTEKEKA LYKDIEFSVE ELNAATGSKT
    310 320 330 340 350
    SLYDKKEDIL MHRWRYPSLS IHGVEGAFSA QGAKTVIPAK VFGKFSIRTV
    360 370 380 390 400
    PDMDSEKLTS LVQKHCDAKF KSLNSPNKCR TELIHDGAYW VSDPFNAQFT
    410 420 430 440 450
    AAKKATKLVY GVDPDFTREG GSIPITLTFQ DALNTSVLLL PMGRGDDGAH
    460 470 480
    SINEKLDISN FVGGMKTMAA YLQYYSESPE N
    Length:481
    Mass (Da):52,871
    Last modified:November 1, 1995 - v1
    Checksum:i3E53773A945F5EBC
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    D50617 Genomic DNA Translation: BAA09283.1
    AY692702 Genomic DNA Translation: AAT92721.1
    BK006940 Genomic DNA Translation: DAA12487.1
    PIRiS56299
    RefSeqiNP_116702.1, NM_001180009.1

    Genome annotation databases

    EnsemblFungiiBAA09283; BAA09283; BAA09283
    YFR044C; YFR044C; YFR044C
    GeneIDi850605
    KEGGisce:YFR044C

    Similar proteinsi

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    D50617 Genomic DNA Translation: BAA09283.1
    AY692702 Genomic DNA Translation: AAT92721.1
    BK006940 Genomic DNA Translation: DAA12487.1
    PIRiS56299
    RefSeqiNP_116702.1, NM_001180009.1

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    4G1PX-ray2.55A1-481[»]
    ProteinModelPortaliP43616
    SMRiP43616
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi31202, 50 interactors
    DIPiDIP-6492N
    IntActiP43616, 4 interactors
    MINTiP43616
    STRINGi4932.YFR044C

    Protein family/group databases

    MEROPSiM20.017

    PTM databases

    iPTMnetiP43616

    Proteomic databases

    MaxQBiP43616
    PaxDbiP43616
    PRIDEiP43616

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblFungiiBAA09283; BAA09283; BAA09283
    YFR044C; YFR044C; YFR044C
    GeneIDi850605
    KEGGisce:YFR044C

    Organism-specific databases

    EuPathDBiFungiDB:YFR044C
    SGDiS000001940 DUG1

    Phylogenomic databases

    GeneTreeiENSGT00390000009682
    HOGENOMiHOG000216709
    InParanoidiP43616
    KOiK15428
    OMAiHITIPGF
    OrthoDBiEOG092C1SDA

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER3O-4
    YEAST:MONOMER3O-4
    ReactomeiR-SCE-174403 Glutathione synthesis and recycling
    SABIO-RKiP43616

    Miscellaneous databases

    PROiPR:P43616

    Family and domain databases

    CDDicd05676 M20_dipept_like_CNDP, 1 hit
    InterProiView protein in InterPro
    IPR017153 CNDP/DUG1
    IPR002933 Peptidase_M20
    IPR011650 Peptidase_M20_dimer
    PfamiView protein in Pfam
    PF07687 M20_dimer, 1 hit
    PF01546 Peptidase_M20, 1 hit
    PIRSFiPIRSF037242 CNDP_dipeptidase, 1 hit
    ProtoNetiSearch...

    Entry informationi

    Entry nameiDUG1_YEAST
    AccessioniPrimary (citable) accession number: P43616
    Secondary accession number(s): D6VTS7
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: September 12, 2018
    This is version 165 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    5. Yeast chromosome VI
      Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names
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