UniProtKB - P43609 (RSC8_YEAST)
Protein
Chromatin structure-remodeling complex protein RSC8
Gene
RSC8
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Component of the chromatin structure-remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton. This subunit is essential for mitotic growth and for repression of CHA1 expression.7 Publications
Miscellaneous
Present with 3380 molecules/cell in log phase SD medium.1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 254 – 298 | ZZ-typeAdd BLAST | 45 |
GO - Molecular functioni
- DNA binding Source: SGD
- histone binding Source: GO_Central
- zinc ion binding Source: InterPro
GO - Biological processi
- ATP-dependent chromatin remodeling Source: UniProtKB
- double-strand break repair via nonhomologous end joining Source: SGD
- nucleosome disassembly Source: SGD
- positive regulation of transcription, DNA-templated Source: GO_Central
- regulation of transcription, DNA-templated Source: UniProtKB
- transcription elongation from RNA polymerase II promoter Source: SGD
Keywordsi
Molecular function | Chromatin regulator, DNA-binding |
Biological process | Transcription, Transcription regulation |
Ligand | Metal-binding, Zinc |
Names & Taxonomyi
Protein namesi | Recommended name: Chromatin structure-remodeling complex protein RSC8Alternative name(s): Remodel the structure of chromatin complex subunit 8 SWI3 homolog |
Gene namesi | Name:RSC8 Synonyms:SWH3 Ordered Locus Names:YFR037C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000001933, RSC8 |
VEuPathDBi | FungiDB:YFR037C |
Subcellular locationi
Nucleus
- Nucleus PROSITE-ProRule annotation1 Publication
Note: Localizes to centromeric and flanking chromatin. Association with these loci is dependent on STH1.
Nucleus
- nucleus Source: SGD
- RSC-type complex Source: UniProtKB
- SWI/SNF complex Source: GO_Central
Keywords - Cellular componenti
NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 347 – 351 | Missing : Loss of function. 1 Publication | 5 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000197114 | 1 – 557 | Chromatin structure-remodeling complex protein RSC8Add BLAST | 557 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 485 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P43609 |
PaxDbi | P43609 |
PRIDEi | P43609 |
PTM databases
iPTMneti | P43609 |
Interactioni
Subunit structurei
Dimerizes via the C-terminal coiled coil.
Interacts directly with HTL1, NPL6, RSC6 and the N-terminus of STH1.
Component of the two forms of the RSC complex composed of at least either RSC1 or RSC2, and ARP7, ARP9, LDB7, NPL6, RSC3, RSC30, RSC4, RSC58, RSC6, RSC8, RSC9, SFH1, STH1, HTL1 and probably RTT102. The complexes interact with histone and histone variant components of centromeric chromatin.
5 PublicationsBinary interactionsi
Hide detailsP43609
With | #Exp. | IntAct |
---|---|---|
HTL1 [Q9URQ5] | 3 | EBI-23005,EBI-8717 |
NPL6 [P32832] | 7 | EBI-23005,EBI-12202 |
RSC6 [P25632] | 12 | EBI-23005,EBI-21941 |
GO - Molecular functioni
- histone binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 31195, 484 interactors |
ComplexPortali | CPX-1888, RSC complex variant RSC2 CPX-1889, RSC complex variant RSC1 |
DIPi | DIP-1478N |
IntActi | P43609, 94 interactors |
MINTi | P43609 |
STRINGi | 4932.YFR037C |
Miscellaneous databases
RNActi | P43609, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P43609 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 80 – 177 | SWIRMPROSITE-ProRule annotationAdd BLAST | 98 | |
Domaini | 310 – 362 | SANTPROSITE-ProRule annotationAdd BLAST | 53 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 38 – 77 | Sequence analysisAdd BLAST | 40 | |
Coiled coili | 462 – 494 | Sequence analysisAdd BLAST | 33 |
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 254 – 298 | ZZ-typeAdd BLAST | 45 |
Keywords - Domaini
Coiled coil, Zinc-fingerPhylogenomic databases
eggNOGi | KOG1279, Eukaryota |
HOGENOMi | CLU_004447_3_2_1 |
InParanoidi | P43609 |
OMAi | TEVRYHN |
Family and domain databases
CDDi | cd02336, ZZ_RSC8, 1 hit |
Gene3Di | 1.10.10.10, 1 hit 3.30.60.90, 1 hit |
InterProi | View protein in InterPro IPR009057, Homeobox-like_sf IPR041984, Rsc8/Ssr1/Ssr2_ZZ IPR001005, SANT/Myb IPR017884, SANT_dom IPR032451, SMARCC_C IPR007526, SWIRM IPR036388, WH-like_DNA-bd_sf IPR000433, Znf_ZZ IPR043145, Znf_ZZ_sf |
Pfami | View protein in Pfam PF00249, Myb_DNA-binding, 1 hit PF04433, SWIRM, 1 hit PF16495, SWIRM-assoc_1, 1 hit PF00569, ZZ, 1 hit |
SMARTi | View protein in SMART SM00717, SANT, 1 hit SM00291, ZnF_ZZ, 1 hit |
SUPFAMi | SSF46689, SSF46689, 2 hits |
PROSITEi | View protein in PROSITE PS51293, SANT, 1 hit PS50934, SWIRM, 1 hit |
i Sequence
Sequence statusi: Complete.
P43609-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSDTEKDKDV PMVDSHEATE EPPTTSTNTP SFPHLAQEQA KEESATLGAE
60 70 80 90 100
VAHKKINYEQ EAQKLEEKAL RFLAKQTHPV IIPSFASWFD ISKIHEIEKR
110 120 130 140 150
SNPDFFNDSS RFKTPKAYKD TRNFIINTYR LSPYEYLTIT AVRRNVAMDV
160 170 180 190 200
ASIVKIHAFL EKWGLINYQI DPRTKPSLIG PSFTGHFQVV LDTPQGLKPF
210 220 230 240 250
LPENVIKQEV EGGDGAEPQV KKEFPVNLTI KKNVYDSAQD FNALQDESRN
260 270 280 290 300
SRQIHKVYIC HTCGNESINV RYHNLRARDT NLCSRCFQEG HFGANFQSSD
310 320 330 340 350
FIRLENNGNS VKKNWSDQEM LLLLEGIEMY EDQWEKIADH VGGHKRVEDC
360 370 380 390 400
IEKFLSLPIE DNYIREVVGS TLNGKGGDSR DGSVSGSKLM ECVNDAVQTL
410 420 430 440 450
LQGDDKLGKV SDKSREISEK YIEESQAIIQ ELVKLTMEKL ESKFTKLCDL
460 470 480 490 500
ETQLEMEKLK YVKESEKMLN DRLSLSKQIL DLNKSLEELN VSKKLVLISE
510 520 530 540 550
QVDSGIQLVE KDQEGDDEDG NTATGHGVKR VGKEGEEVGE GDSIAKLQPQ
VYKPWSL
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 192 | D → G in AAT92860 (PubMed:17322287).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D50617 Genomic DNA Translation: BAA09276.1 AY692841 Genomic DNA Translation: AAT92860.1 BK006940 Genomic DNA Translation: DAA12480.1 |
PIRi | S56292 |
RefSeqi | NP_116695.3, NM_001180002.3 |
Genome annotation databases
EnsemblFungii | YFR037C_mRNA; YFR037C; YFR037C |
GeneIDi | 850598 |
KEGGi | sce:YFR037C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D50617 Genomic DNA Translation: BAA09276.1 AY692841 Genomic DNA Translation: AAT92860.1 BK006940 Genomic DNA Translation: DAA12480.1 |
PIRi | S56292 |
RefSeqi | NP_116695.3, NM_001180002.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6K15 | electron microscopy | 3.40 | D/H | 1-557 | [»] | |
6KW3 | electron microscopy | 7.13 | D/H | 1-557 | [»] | |
6KW4 | electron microscopy | 7.55 | D/H | 1-557 | [»] | |
6KW5 | electron microscopy | 10.13 | D/H | 1-557 | [»] | |
6TDA | electron microscopy | 15.00 | L | 1-557 | [»] | |
6V8O | electron microscopy | 3.07 | I/J/K/L | 1-557 | [»] | |
6V92 | electron microscopy | 20.00 | I/J/K/L | 1-557 | [»] | |
SMRi | P43609 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 31195, 484 interactors |
ComplexPortali | CPX-1888, RSC complex variant RSC2 CPX-1889, RSC complex variant RSC1 |
DIPi | DIP-1478N |
IntActi | P43609, 94 interactors |
MINTi | P43609 |
STRINGi | 4932.YFR037C |
PTM databases
iPTMneti | P43609 |
Proteomic databases
MaxQBi | P43609 |
PaxDbi | P43609 |
PRIDEi | P43609 |
Genome annotation databases
EnsemblFungii | YFR037C_mRNA; YFR037C; YFR037C |
GeneIDi | 850598 |
KEGGi | sce:YFR037C |
Organism-specific databases
SGDi | S000001933, RSC8 |
VEuPathDBi | FungiDB:YFR037C |
Phylogenomic databases
eggNOGi | KOG1279, Eukaryota |
HOGENOMi | CLU_004447_3_2_1 |
InParanoidi | P43609 |
OMAi | TEVRYHN |
Miscellaneous databases
PROi | PR:P43609 |
RNActi | P43609, protein |
Family and domain databases
CDDi | cd02336, ZZ_RSC8, 1 hit |
Gene3Di | 1.10.10.10, 1 hit 3.30.60.90, 1 hit |
InterProi | View protein in InterPro IPR009057, Homeobox-like_sf IPR041984, Rsc8/Ssr1/Ssr2_ZZ IPR001005, SANT/Myb IPR017884, SANT_dom IPR032451, SMARCC_C IPR007526, SWIRM IPR036388, WH-like_DNA-bd_sf IPR000433, Znf_ZZ IPR043145, Znf_ZZ_sf |
Pfami | View protein in Pfam PF00249, Myb_DNA-binding, 1 hit PF04433, SWIRM, 1 hit PF16495, SWIRM-assoc_1, 1 hit PF00569, ZZ, 1 hit |
SMARTi | View protein in SMART SM00717, SANT, 1 hit SM00291, ZnF_ZZ, 1 hit |
SUPFAMi | SSF46689, SSF46689, 2 hits |
PROSITEi | View protein in PROSITE PS51293, SANT, 1 hit PS50934, SWIRM, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RSC8_YEAST | |
Accessioni | P43609Primary (citable) accession number: P43609 Secondary accession number(s): D6VTS0, Q6B289 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | November 1, 1995 | |
Last modified: | April 7, 2021 | |
This is version 194 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome VI
Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references