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Protein

Chromatin structure-remodeling complex protein RSC8

Gene

RSC8

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the chromatin structure-remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton. This subunit is essential for mitotic growth and for repression of CHA1 expression.7 Publications

Miscellaneous

Present with 3380 molecules/cell in log phase SD medium.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri254 – 298ZZ-typeAdd BLAST45

GO - Molecular functioni

  • DNA binding Source: SGD
  • zinc ion binding Source: InterPro

GO - Biological processi

  • ATP-dependent chromatin remodeling Source: UniProtKB
  • double-strand break repair via nonhomologous end joining Source: SGD
  • nucleosome disassembly Source: SGD
  • regulation of transcription, DNA-templated Source: UniProtKB
  • transcription elongation from RNA polymerase II promoter Source: SGD

Keywordsi

Molecular functionChromatin regulator, DNA-binding
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-30484-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Chromatin structure-remodeling complex protein RSC8
Alternative name(s):
Remodel the structure of chromatin complex subunit 8
SWI3 homolog
Gene namesi
Name:RSC8
Synonyms:SWH3
Ordered Locus Names:YFR037C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VI

Organism-specific databases

EuPathDBiFungiDB:YFR037C
SGDiS000001933 RSC8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi347 – 351Missing : Loss of function. 1 Publication5

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001971141 – 557Chromatin structure-remodeling complex protein RSC8Add BLAST557

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei485PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP43609
PaxDbiP43609
PRIDEiP43609

PTM databases

iPTMnetiP43609

Interactioni

Subunit structurei

Dimerizes via the C-terminal coiled coil. Interacts directly with HTL1, NPL6, RSC6 and the N-terminus of STH1. Component of the two forms of the RSC complex composed of at least either RSC1 or RSC2, and ARP7, ARP9, LDB7, NPL6, RSC3, RSC30, RSC4, RSC58, RSC6, RSC8, RSC9, SFH1, STH1, HTL1 and probably RTT102. The complexes interact with histone and histone variant components of centromeric chromatin.5 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi31195, 480 interactors
ComplexPortaliCPX-1888 RSC complex variant RSC2
CPX-1889 RSC complex variant RSC1
DIPiDIP-1478N
IntActiP43609, 93 interactors
MINTiP43609
STRINGi4932.YFR037C

Structurei

3D structure databases

ProteinModelPortaliP43609
SMRiP43609
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini80 – 177SWIRMPROSITE-ProRule annotationAdd BLAST98
Domaini310 – 362SANTPROSITE-ProRule annotationAdd BLAST53

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili38 – 77Sequence analysisAdd BLAST40
Coiled coili462 – 494Sequence analysisAdd BLAST33

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri254 – 298ZZ-typeAdd BLAST45

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

HOGENOMiHOG000246692
InParanoidiP43609
KOiK11762
OMAiVRYHNLK
OrthoDBiEOG092C5VLW

Family and domain databases

CDDicd00167 SANT, 1 hit
Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR009057 Homeobox-like_sf
IPR001005 SANT/Myb
IPR017884 SANT_dom
IPR032451 SMARCC_C
IPR007526 SWIRM
IPR036388 WH-like_DNA-bd_sf
IPR000433 Znf_ZZ
PfamiView protein in Pfam
PF00249 Myb_DNA-binding, 1 hit
PF04433 SWIRM, 1 hit
PF16495 SWIRM-assoc_1, 1 hit
PF00569 ZZ, 1 hit
SMARTiView protein in SMART
SM00717 SANT, 1 hit
SM00291 ZnF_ZZ, 1 hit
SUPFAMiSSF46689 SSF46689, 2 hits
PROSITEiView protein in PROSITE
PS51293 SANT, 1 hit
PS50934 SWIRM, 1 hit

Sequencei

Sequence statusi: Complete.

P43609-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDTEKDKDV PMVDSHEATE EPPTTSTNTP SFPHLAQEQA KEESATLGAE
60 70 80 90 100
VAHKKINYEQ EAQKLEEKAL RFLAKQTHPV IIPSFASWFD ISKIHEIEKR
110 120 130 140 150
SNPDFFNDSS RFKTPKAYKD TRNFIINTYR LSPYEYLTIT AVRRNVAMDV
160 170 180 190 200
ASIVKIHAFL EKWGLINYQI DPRTKPSLIG PSFTGHFQVV LDTPQGLKPF
210 220 230 240 250
LPENVIKQEV EGGDGAEPQV KKEFPVNLTI KKNVYDSAQD FNALQDESRN
260 270 280 290 300
SRQIHKVYIC HTCGNESINV RYHNLRARDT NLCSRCFQEG HFGANFQSSD
310 320 330 340 350
FIRLENNGNS VKKNWSDQEM LLLLEGIEMY EDQWEKIADH VGGHKRVEDC
360 370 380 390 400
IEKFLSLPIE DNYIREVVGS TLNGKGGDSR DGSVSGSKLM ECVNDAVQTL
410 420 430 440 450
LQGDDKLGKV SDKSREISEK YIEESQAIIQ ELVKLTMEKL ESKFTKLCDL
460 470 480 490 500
ETQLEMEKLK YVKESEKMLN DRLSLSKQIL DLNKSLEELN VSKKLVLISE
510 520 530 540 550
QVDSGIQLVE KDQEGDDEDG NTATGHGVKR VGKEGEEVGE GDSIAKLQPQ

VYKPWSL
Length:557
Mass (Da):63,168
Last modified:November 1, 1995 - v1
Checksum:iD3D3120397CF0058
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti192D → G in AAT92860 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50617 Genomic DNA Translation: BAA09276.1
AY692841 Genomic DNA Translation: AAT92860.1
BK006940 Genomic DNA Translation: DAA12480.1
PIRiS56292
RefSeqiNP_116695.3, NM_001180002.3

Genome annotation databases

EnsemblFungiiBAA09276; BAA09276; BAA09276
YFR037C; YFR037C; YFR037C
GeneIDi850598
KEGGisce:YFR037C

Similar proteinsi

Entry informationi

Entry nameiRSC8_YEAST
AccessioniPrimary (citable) accession number: P43609
Secondary accession number(s): D6VTS0, Q6B289
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: June 20, 2018
This is version 175 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome VI
    Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

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