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Protein

N-acetyltransferase ECO1

Gene

ECO1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for establishment of sister chromatid cohesion during S phase but not for its further maintenance during G2 or M phases or for loading the cohesin complex onto DNA. Interacts with the three known alternate replication factor C (RFC) complexes, suggesting that these complexes have essential but redundant activity in cohesion establishment. Acts by acetylating the cohesin complex component SMC3. In vitro, possesses acetyltransferase activity where it can acetylate itself and components of the cohesin complex (MCD1, IRR1 and PDS5), but is unable to acetylate histones.4 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri33 – 57CCHH-typeAdd BLAST25

GO - Molecular functioni

  • acetyltransferase activity Source: UniProtKB
  • chromatin binding Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • chromosome organization Source: SGD
  • DNA repair Source: SGD
  • DNA replication Source: SGD
  • double-strand break repair Source: SGD
  • establishment of mitotic sister chromatid cohesion Source: SGD
  • internal peptidyl-lysine acetylation Source: SGD
  • meiotic chromosome segregation Source: InterPro
  • mitotic chromosome condensation Source: SGD
  • regulation of mitotic nuclear division Source: UniProtKB
  • telomere organization Source: SGD
  • tRNA gene clustering Source: SGD

Keywordsi

Molecular functionAcyltransferase, Transferase
Biological processCell cycle, DNA replication
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-30476-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
N-acetyltransferase ECO1 (EC:2.3.1.-)
Alternative name(s):
Chromosome transmission fidelity protein 7
Establishment of cohesion protein 1
Gene namesi
Name:ECO1
Synonyms:CTF7
Ordered Locus Names:YFR027W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VI

Organism-specific databases

EuPathDBiFungiDB:YFR027W
SGDiS000001923 ECO1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi35C → Y in ctf7-109; loss of function. 1 Publication1
Mutagenesisi53H → Y in ctf7-108; loss of function. 1 Publication1
Mutagenesisi211G → D: Abolishes acetyltransferase activity; but not chromatid cohesion activity. 1 Publication1
Mutagenesisi222 – 223RK → GG: Abolishes acetyltransferase activity; but not chromatid cohesion activity. 1 Publication2
Mutagenesisi225G → D: Abolishes acetyltransferase activity; but not chromatid cohesion activity. 1 Publication1
Mutagenesisi232D → G: Abolishes acetyltransferase activity; but not chromatid cohesion activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000745501 – 281N-acetyltransferase ECO1Add BLAST281

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei223N6-acetyllysine; by autocatalysis1 Publication1

Post-translational modificationi

Autoacetylates in vitro.

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP43605
PaxDbiP43605
PRIDEiP43605

PTM databases

iPTMnetiP43605

Interactioni

Subunit structurei

Binds specifically to CHL12, RFC1, RFC2, RFC3, RFC4, RFC5 and RAD24 when members of an RFC complex. Interacts with CHL1 and MPS3.4 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi31181, 128 interactors
DIPiDIP-5602N
IntActiP43605, 14 interactors
STRINGi4932.YFR027W

Structurei

3D structure databases

ProteinModelPortaliP43605
SMRiP43605
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the acetyltransferase family. ECO subfamily.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri33 – 57CCHH-typeAdd BLAST25

Keywords - Domaini

Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00390000008335
HOGENOMiHOG000093358
InParanoidiP43605
KOiK11268
OMAiSRIWVCR
OrthoDBiEOG092C4EXV

Family and domain databases

InterProiView protein in InterPro
IPR028005 AcTrfase_ESCO_Znf_dom
IPR016181 Acyl_CoA_acyltransferase
IPR033257 Eco1/CTF7
IPR028009 ESCO_Acetyltransf_dom
PANTHERiPTHR11076:SF1 PTHR11076:SF1, 1 hit
PfamiView protein in Pfam
PF13880 Acetyltransf_13, 1 hit
PF13878 zf-C2H2_3, 1 hit
SUPFAMiSSF55729 SSF55729, 1 hit

Sequencei

Sequence statusi: Complete.

P43605-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKARKSQRKA GSKPNLIQSK LQVNNGSKSN KIVKCDKCEM SYSSTSIEDR
60 70 80 90 100
AIHEKYHTLQ LHGRKWSPNW GSIVYTERNH SRTVHLSRST GTITPLNSSP
110 120 130 140 150
LKKSSPSITH QEEKIVYVRP DKSNGEVRAM TEIMTLVNNE LNAPHDENVI
160 170 180 190 200
WNSTTEEKGK AFVYIRNDRA VGIIIIENLY GGNGKTSSRG RWMVYDSRRL
210 220 230 240 250
VQNVYPDFKI GISRIWVCRT ARKLGIATKL IDVARENIVY GEVIPRYQVA
260 270 280
WSQPTDSGGK LASKYNGIMH KSGKLLLPVY I
Length:281
Mass (Da):31,845
Last modified:November 1, 1995 - v1
Checksum:i0AF1B59BE9E71818
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50617 Genomic DNA Translation: BAA09266.1
BK006940 Genomic DNA Translation: DAA12467.1
PIRiS56282
RefSeqiNP_116683.1, NM_001179992.1

Genome annotation databases

EnsemblFungiiBAA09266; BAA09266; BAA09266
YFR027W; YFR027W; YFR027W
GeneIDi850584
KEGGisce:YFR027W

Similar proteinsi

Entry informationi

Entry nameiECO1_YEAST
AccessioniPrimary (citable) accession number: P43605
Secondary accession number(s): D6VTQ7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: June 20, 2018
This is version 149 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VI
    Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

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