UniProtKB - P43605 (ECO1_YEAST)
N-acetyltransferase ECO1
ECO1
Functioni
Required for establishment of sister chromatid cohesion during S phase but not for its further maintenance during G2 or M phases or for loading the cohesin complex onto DNA. Interacts with the three known alternate replication factor C (RFC) complexes, suggesting that these complexes have essential but redundant activity in cohesion establishment. Acts by acetylating the cohesin complex component SMC3. In vitro, possesses acetyltransferase activity where it can acetylate itself and components of the cohesin complex (MCD1, IRR1 and PDS5), but is unable to acetylate histones.
4 PublicationsRegions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 33 – 57 | CCHH-typeAdd BLAST | 25 |
GO - Molecular functioni
- acetyltransferase activity Source: UniProtKB
- chromatin binding Source: UniProtKB
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- chromatin looping Source: SGD
- chromosome organization Source: SGD
- DNA repair Source: SGD
- DNA replication Source: SGD
- double-strand break repair Source: SGD
- establishment of mitotic sister chromatid cohesion Source: SGD
- internal peptidyl-lysine acetylation Source: SGD
- meiotic chromosome segregation Source: InterPro
- mitotic chromosome condensation Source: SGD
- post-translational protein acetylation Source: GO_Central
- regulation of DNA replication Source: GO_Central
- regulation of mitotic nuclear division Source: UniProtKB
- sister chromatid cohesion Source: GO_Central
- telomere organization Source: SGD
- tRNA gene clustering Source: SGD
Keywordsi
Molecular function | Acyltransferase, Transferase |
Biological process | Cell cycle, DNA replication |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-SCE-2468052, Establishment of Sister Chromatid Cohesion |
Names & Taxonomyi
Protein namesi | Recommended name: N-acetyltransferase ECO1 (EC:2.3.1.-)Alternative name(s): Chromosome transmission fidelity protein 7 Establishment of cohesion protein 1 |
Gene namesi | Name:ECO1 Synonyms:CTF7 Ordered Locus Names:YFR027W |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000001923, ECO1 |
VEuPathDBi | FungiDB:YFR027W |
Subcellular locationi
Nucleus
- Nucleus 2 Publications
Note: Associated with chromatin.
Nucleus
- nuclear replication fork Source: SGD
- nucleus Source: SGD
Other locations
Keywords - Cellular componenti
NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 35 | C → Y in ctf7-109; loss of function. 1 Publication | 1 | |
Mutagenesisi | 53 | H → Y in ctf7-108; loss of function. 1 Publication | 1 | |
Mutagenesisi | 211 | G → D: Abolishes acetyltransferase activity; but not chromatid cohesion activity. 1 Publication | 1 | |
Mutagenesisi | 222 – 223 | RK → GG: Abolishes acetyltransferase activity; but not chromatid cohesion activity. 1 Publication | 2 | |
Mutagenesisi | 225 | G → D: Abolishes acetyltransferase activity; but not chromatid cohesion activity. 1 Publication | 1 | |
Mutagenesisi | 232 | D → G: Abolishes acetyltransferase activity; but not chromatid cohesion activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000074550 | 1 – 281 | N-acetyltransferase ECO1Add BLAST | 281 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 223 | N6-acetyllysine; by autocatalysis1 Publication | 1 |
Post-translational modificationi
Keywords - PTMi
AcetylationProteomic databases
MaxQBi | P43605 |
PaxDbi | P43605 |
PTM databases
iPTMneti | P43605 |
Interactioni
Subunit structurei
Binds specifically to CHL12, RFC1, RFC2, RFC3, RFC4, RFC5 and RAD24 when members of an RFC complex.
Interacts with CHL1 and MPS3.
4 PublicationsBinary interactionsi
P43605
With | #Exp. | IntAct |
---|---|---|
CHL1 [P22516] | 2 | EBI-22988,EBI-4600 |
CTF18 [P49956] | 2 | EBI-22988,EBI-4560 |
MPS3 [P47069] | 5 | EBI-22988,EBI-25811 |
POL30 [P15873] | 2 | EBI-22988,EBI-12993 |
Protein-protein interaction databases
BioGRIDi | 31181, 142 interactors |
DIPi | DIP-5602N |
IntActi | P43605, 14 interactors |
STRINGi | 4932.YFR027W |
Miscellaneous databases
RNActi | P43605, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 86 – 105 | DisorderedSequence analysisAdd BLAST | 20 |
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 33 – 57 | CCHH-typeAdd BLAST | 25 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG3014, Eukaryota |
GeneTreei | ENSGT00940000175408 |
HOGENOMi | CLU_039183_2_1_1 |
InParanoidi | P43605 |
OMAi | SRIWVCR |
Family and domain databases
InterProi | View protein in InterPro IPR028005, AcTrfase_ESCO_Znf_dom IPR016181, Acyl_CoA_acyltransferase IPR033257, Eco1/CTF7 IPR028009, ESCO_Acetyltransf_dom |
PANTHERi | PTHR45884:SF2, PTHR45884:SF2, 1 hit |
Pfami | View protein in Pfam PF13880, Acetyltransf_13, 1 hit PF13878, zf-C2H2_3, 1 hit |
SUPFAMi | SSF55729, SSF55729, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MKARKSQRKA GSKPNLIQSK LQVNNGSKSN KIVKCDKCEM SYSSTSIEDR
60 70 80 90 100
AIHEKYHTLQ LHGRKWSPNW GSIVYTERNH SRTVHLSRST GTITPLNSSP
110 120 130 140 150
LKKSSPSITH QEEKIVYVRP DKSNGEVRAM TEIMTLVNNE LNAPHDENVI
160 170 180 190 200
WNSTTEEKGK AFVYIRNDRA VGIIIIENLY GGNGKTSSRG RWMVYDSRRL
210 220 230 240 250
VQNVYPDFKI GISRIWVCRT ARKLGIATKL IDVARENIVY GEVIPRYQVA
260 270 280
WSQPTDSGGK LASKYNGIMH KSGKLLLPVY I
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D50617 Genomic DNA Translation: BAA09266.1 BK006940 Genomic DNA Translation: DAA12467.1 |
PIRi | S56282 |
RefSeqi | NP_116683.1, NM_001179992.1 |
Genome annotation databases
EnsemblFungii | YFR027W_mRNA; YFR027W; YFR027W |
GeneIDi | 850584 |
KEGGi | sce:YFR027W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D50617 Genomic DNA Translation: BAA09266.1 BK006940 Genomic DNA Translation: DAA12467.1 |
PIRi | S56282 |
RefSeqi | NP_116683.1, NM_001179992.1 |
3D structure databases
SMRi | P43605 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 31181, 142 interactors |
DIPi | DIP-5602N |
IntActi | P43605, 14 interactors |
STRINGi | 4932.YFR027W |
PTM databases
iPTMneti | P43605 |
Proteomic databases
MaxQBi | P43605 |
PaxDbi | P43605 |
Genome annotation databases
EnsemblFungii | YFR027W_mRNA; YFR027W; YFR027W |
GeneIDi | 850584 |
KEGGi | sce:YFR027W |
Organism-specific databases
SGDi | S000001923, ECO1 |
VEuPathDBi | FungiDB:YFR027W |
Phylogenomic databases
eggNOGi | KOG3014, Eukaryota |
GeneTreei | ENSGT00940000175408 |
HOGENOMi | CLU_039183_2_1_1 |
InParanoidi | P43605 |
OMAi | SRIWVCR |
Enzyme and pathway databases
Reactomei | R-SCE-2468052, Establishment of Sister Chromatid Cohesion |
Miscellaneous databases
PROi | PR:P43605 |
RNActi | P43605, protein |
Family and domain databases
InterProi | View protein in InterPro IPR028005, AcTrfase_ESCO_Znf_dom IPR016181, Acyl_CoA_acyltransferase IPR033257, Eco1/CTF7 IPR028009, ESCO_Acetyltransf_dom |
PANTHERi | PTHR45884:SF2, PTHR45884:SF2, 1 hit |
Pfami | View protein in Pfam PF13880, Acetyltransf_13, 1 hit PF13878, zf-C2H2_3, 1 hit |
SUPFAMi | SSF55729, SSF55729, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ECO1_YEAST | |
Accessioni | P43605Primary (citable) accession number: P43605 Secondary accession number(s): D6VTQ7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | November 1, 1995 | |
Last modified: | February 23, 2022 | |
This is version 169 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome VI
Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names - SIMILARITY comments
Index of protein domains and families