UniProtKB - P43577 (GNA1_YEAST)
Protein
Glucosamine 6-phosphate N-acetyltransferase
Gene
GNA1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Miscellaneous
Present with 2550 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
- EC:2.3.1.41 Publication
: UDP-N-acetyl-alpha-D-glucosamine biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes N-acetyl-alpha-D-glucosamine 1-phosphate from alpha-D-glucosamine 6-phosphate (route I).Proteins known to be involved in the 2 steps of the subpathway in this organism are:
- Glucosamine 6-phosphate N-acetyltransferase (GNA1)
- Phosphoacetylglucosamine mutase (PCM1)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N-acetyl-alpha-D-glucosamine 1-phosphate from alpha-D-glucosamine 6-phosphate (route I), the pathway UDP-N-acetyl-alpha-D-glucosamine biosynthesis and in Nucleotide-sugar biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 28 | Substrate1 Publication | 1 | |
Binding sitei | 134 | Substrate; via carbonyl oxygen1 Publication | 1 | |
Binding sitei | 158 | Substrate1 Publication | 1 |
GO - Molecular functioni
- glucosamine 6-phosphate N-acetyltransferase activity Source: SGD
- monosaccharide binding Source: GO_Central
- N-acetyltransferase activity Source: GO_Central
GO - Biological processi
- UDP-N-acetylglucosamine biosynthetic process Source: SGD
Keywordsi
Molecular function | Acyltransferase, Transferase |
Enzyme and pathway databases
BioCyci | MetaCyc:YFL017C-MONOMER |
Reactomei | R-SCE-446210, Synthesis of UDP-N-acetyl-glucosamine |
SABIO-RKi | P43577 |
UniPathwayi | UPA00113;UER00529 |
Names & Taxonomyi
Protein namesi | Recommended name: Glucosamine 6-phosphate N-acetyltransferase (EC:2.3.1.41 Publication)Alternative name(s): Phosphoglucosamine acetylase Phosphoglucosamine transacetylase |
Gene namesi | Name:GNA1 Synonyms:PAT1 Ordered Locus Names:YFL017C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000001877, GNA1 |
VEuPathDBi | FungiDB:YFL017C |
Subcellular locationi
Endoplasmic reticulum
- endoplasmic reticulum Source: GO_Central
Golgi apparatus
- Golgi apparatus Source: GO_Central
Nucleus
- nucleus Source: SGD
Other locations
- cytoplasm Source: SGD
- endoplasmic reticulum-Golgi intermediate compartment Source: GO_Central
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000074552 | 2 – 159 | Glucosamine 6-phosphate N-acetyltransferaseAdd BLAST | 158 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineCombined sources | 1 |
Keywords - PTMi
AcetylationProteomic databases
MaxQBi | P43577 |
PaxDbi | P43577 |
PRIDEi | P43577 |
PTM databases
iPTMneti | P43577 |
Interactioni
Subunit structurei
Homodimer.
1 PublicationProtein-protein interaction databases
BioGRIDi | 31130, 309 interactors |
DIPi | DIP-1349N |
IntActi | P43577, 7 interactors |
MINTi | P43577 |
STRINGi | 4932.YFL017C |
Miscellaneous databases
RNActi | P43577, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P43577 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P43577 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 28 – 159 | N-acetyltransferasePROSITE-ProRule annotationAdd BLAST | 132 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 86 – 89 | Substrate binding1 Publication | 4 | |
Regioni | 98 – 100 | Substrate binding1 Publication | 3 | |
Regioni | 100 – 102 | Acetyl-CoA bindingCombined sources1 Publication | 3 | |
Regioni | 108 – 113 | Acetyl-CoA bindingCombined sources1 Publication | 6 | |
Regioni | 129 – 130 | Substrate binding1 Publication | 2 | |
Regioni | 143 – 145 | Acetyl-CoA bindingCombined sources1 Publication | 3 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG3396, Eukaryota |
GeneTreei | ENSGT00390000008666 |
HOGENOMi | CLU_072095_0_1_1 |
InParanoidi | P43577 |
OMAi | FYIKCGF |
Family and domain databases
InterProi | View protein in InterPro IPR016181, Acyl_CoA_acyltransferase IPR000182, GNAT_dom IPR039143, GNPNAT1 |
PANTHERi | PTHR13355, PTHR13355, 1 hit |
Pfami | View protein in Pfam PF00583, Acetyltransf_1, 1 hit |
SUPFAMi | SSF55729, SSF55729, 1 hit |
PROSITEi | View protein in PROSITE PS51186, GNAT, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P43577-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSLPDGFYIR RMEEGDLEQV TETLKVLTTV GTITPESFSK LIKYWNEATV
60 70 80 90 100
WNDNEDKKIM QYNPMVIVDK RTETVAATGN IIIERKIIHE LGLCGHIEDI
110 120 130 140 150
AVNSKYQGQG LGKLLIDQLV TIGFDYGCYK IILDCDEKNV KFYEKCGFSN
AGVEMQIRK
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 112 – 159 | GKLLI…MQIRK → ASS in CAA86352 (Ref. 4) CuratedAdd BLAST | 48 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB017626 Genomic DNA Translation: BAA36495.1 D50617 Genomic DNA Translation: BAA09221.1 Z46255 Genomic DNA Translation: CAA86352.1 AY558564 Genomic DNA Translation: AAS56890.1 BK006940 Genomic DNA Translation: DAA12423.1 |
PIRi | S56237 |
RefSeqi | NP_116637.1, NM_001179949.1 |
Genome annotation databases
EnsemblFungii | YFL017C_mRNA; YFL017C; YFL017C |
GeneIDi | 850529 |
KEGGi | sce:YFL017C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB017626 Genomic DNA Translation: BAA36495.1 D50617 Genomic DNA Translation: BAA09221.1 Z46255 Genomic DNA Translation: CAA86352.1 AY558564 Genomic DNA Translation: AAS56890.1 BK006940 Genomic DNA Translation: DAA12423.1 |
PIRi | S56237 |
RefSeqi | NP_116637.1, NM_001179949.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1I12 | X-ray | 1.30 | A/B/C/D | 1-159 | [»] | |
1I1D | X-ray | 1.80 | A/B/C/D | 1-159 | [»] | |
1I21 | X-ray | 2.40 | A/B/M/N/X/Y | 1-159 | [»] | |
SMRi | P43577 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 31130, 309 interactors |
DIPi | DIP-1349N |
IntActi | P43577, 7 interactors |
MINTi | P43577 |
STRINGi | 4932.YFL017C |
PTM databases
iPTMneti | P43577 |
Proteomic databases
MaxQBi | P43577 |
PaxDbi | P43577 |
PRIDEi | P43577 |
Genome annotation databases
EnsemblFungii | YFL017C_mRNA; YFL017C; YFL017C |
GeneIDi | 850529 |
KEGGi | sce:YFL017C |
Organism-specific databases
SGDi | S000001877, GNA1 |
VEuPathDBi | FungiDB:YFL017C |
Phylogenomic databases
eggNOGi | KOG3396, Eukaryota |
GeneTreei | ENSGT00390000008666 |
HOGENOMi | CLU_072095_0_1_1 |
InParanoidi | P43577 |
OMAi | FYIKCGF |
Enzyme and pathway databases
UniPathwayi | UPA00113;UER00529 |
BioCyci | MetaCyc:YFL017C-MONOMER |
Reactomei | R-SCE-446210, Synthesis of UDP-N-acetyl-glucosamine |
SABIO-RKi | P43577 |
Miscellaneous databases
EvolutionaryTracei | P43577 |
PROi | PR:P43577 |
RNActi | P43577, protein |
Family and domain databases
InterProi | View protein in InterPro IPR016181, Acyl_CoA_acyltransferase IPR000182, GNAT_dom IPR039143, GNPNAT1 |
PANTHERi | PTHR13355, PTHR13355, 1 hit |
Pfami | View protein in Pfam PF00583, Acetyltransf_1, 1 hit |
SUPFAMi | SSF55729, SSF55729, 1 hit |
PROSITEi | View protein in PROSITE PS51186, GNAT, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GNA1_YEAST | |
Accessioni | P43577Primary (citable) accession number: P43577 Secondary accession number(s): D6VTL3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | November 1, 1995 | |
Last modified: | February 10, 2021 | |
This is version 179 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Yeast chromosome VI
Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names