UniProtKB - P43535 (GCN20_YEAST)
Protein GCN20
GCN20
Functioni
Miscellaneous
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 232 – 239 | ATP 1PROSITE-ProRule annotation | 8 | |
Nucleotide bindingi | 565 – 572 | ATP 2PROSITE-ProRule annotation | 8 |
GO - Molecular functioni
- ATPase activity Source: SGD
- ATP binding Source: GO_Central
- ribosome binding Source: UniProtKB
- translation initiation factor binding Source: UniProtKB
GO - Biological processi
- cellular response to histidine Source: UniProtKB
- positive regulation of phosphorylation Source: UniProtKB
- positive regulation of translational initiation in response to starvation Source: UniProtKB
- regulation of translational elongation Source: SGD
Keywordsi
Molecular function | Activator |
Biological process | Stress response, Translation regulation |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Protein GCN201 PublicationAlternative name(s): General control non-derepressible protein 20Imported |
Gene namesi | Name:GCN20Imported Ordered Locus Names:YFR009W |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000001905, GCN20 |
VEuPathDBi | FungiDB:YFR009W |
Subcellular locationi
Pathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000093461 | 2 – 752 | Protein GCN20Add BLAST | 751 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineCombined sources | 1 |
Keywords - PTMi
AcetylationProteomic databases
MaxQBi | P43535 |
PaxDbi | P43535 |
PRIDEi | P43535 |
PTM databases
iPTMneti | P43535 |
Interactioni
Subunit structurei
Interacts (via N-terminus) with GCN1 (via C-terminus); this interaction stimulates GCN2 kinase activity in response to amino acid starvation (PubMed:7621831, PubMed:9234705, PubMed:11101534, PubMed:15722345). The GCN1-GCN20 complex interacts with GCN2 on translating ribosomes in amino acid-starved cells; this association stimulates GCN2 kinase activation by uncharged tRNAs, and hence allowing GCN4 translational activation and derepression of amino acid biosynthetic genes (PubMed:7621831, PubMed:9234705, PubMed:10775272, PubMed:11101534). Associates with ribosomes (PubMed:7621831, PubMed:9234705, PubMed:15722345).
5 PublicationsBinary interactionsi
P43535
With | #Exp. | IntAct |
---|---|---|
GCN1 [P33892] | 3 | EBI-7423,EBI-7442 |
GO - Molecular functioni
- translation initiation factor binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 31159, 343 interactors |
ComplexPortali | CPX-1808, GCN1-GCN20 complex |
DIPi | DIP-2345N |
IntActi | P43535, 54 interactors |
MINTi | P43535 |
STRINGi | 4932.YFR009W |
Miscellaneous databases
RNActi | P43535, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 199 – 464 | ABC transporter 1PROSITE-ProRule annotationAdd BLAST | 266 | |
Domaini | 532 – 748 | ABC transporter 2PROSITE-ProRule annotationAdd BLAST | 217 |
Sequence similaritiesi
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG0062, Eukaryota |
GeneTreei | ENSGT00940000155604 |
HOGENOMi | CLU_000604_36_6_1 |
InParanoidi | P43535 |
OMAi | GNYTFWY |
Family and domain databases
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR032781, ABC_tran_Xtn IPR003439, ABC_transporter-like IPR017871, ABC_transporter_CS IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF00005, ABC_tran, 2 hits PF12848, ABC_tran_Xtn, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 2 hits |
SUPFAMi | SSF52540, SSF52540, 2 hits |
PROSITEi | View protein in PROSITE PS00211, ABC_TRANSPORTER_1, 1 hit PS50893, ABC_TRANSPORTER_2, 2 hits |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MASIGSQVRK AASSIDPIVT DYAVGYFNHL SGITFDAVQS KQVDLSTEVQ
60 70 80 90 100
FVSDLLIDAG ASKAKVKELS ESILKQLTTQ LKENEAKLEL TGDTSKRLLD
110 120 130 140 150
INVLKSHNSK SDINVSLSML GVNGDIEHTG RKMETRVDLK KLAKAEQKIA
160 170 180 190 200
KKVAKRNNKF VKYEASKLIN DQKEEDYDSF FLQINPLEFG SSAGKSKDIH
210 220 230 240 250
IDTFDLYVGD GQRILSNAQL TLSFGHRYGL VGQNGIGKST LLRALSRREL
260 270 280 290 300
NVPKHVSILH VEQELRGDDT KALQSVLDAD VWRKQLLSEE AKINERLKEM
310 320 330 340 350
DVLRQEFEED SLEVKKLDNE REDLDNHLIQ ISDKLVDMES DKAEARAASI
360 370 380 390 400
LYGLGFSTEA QQQPTNSFSG GWRMRLSLAR ALFCQPDLLL LDEPSNMLDV
410 420 430 440 450
PSIAYLAEYL KTYPNTVLTV SHDRAFLNEV ATDIIYQHNE RLDYYRGQDF
460 470 480 490 500
DTFYTTKEER RKNAQREYDN QMVYRKHLQE FIDKYRYNAA KSQEAQSRIK
510 520 530 540 550
KLEKLPVLEP PEQDKTIDFK FPECDKLSPP IIQLQDVSFG YDENNLLLKD
560 570 580 590 600
VNLDVQMDSR IALVGANGCG KTTLLKIMME QLRPLKGFVS RNPRLRIGYF
610 620 630 640 650
TQHHVDSMDL TTSAVDWMSK SFPGKTDEEY RRHLGSFGIT GTLGLQKMQL
660 670 680 690 700
LSGGQKSRVA FAALCLNNPH ILVLDEPSNH LDTTGLDALV EALKNFNGGV
710 720 730 740 750
LMVSHDISVI DSVCKEIWVS EQGTVKRFEG TIYDYRDYIL QSADAAGVVK
KH
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U19971 Genomic DNA Translation: AAA75444.1 D50617 Genomic DNA Translation: BAA09248.1 AY723804 Genomic DNA Translation: AAU09721.1 BK006940 Genomic DNA Translation: DAA12449.1 |
PIRi | S56146 |
RefSeqi | NP_116664.1, NM_001179974.1 |
Genome annotation databases
EnsemblFungii | YFR009W_mRNA; YFR009W; YFR009W |
GeneIDi | 850561 |
KEGGi | sce:YFR009W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U19971 Genomic DNA Translation: AAA75444.1 D50617 Genomic DNA Translation: BAA09248.1 AY723804 Genomic DNA Translation: AAU09721.1 BK006940 Genomic DNA Translation: DAA12449.1 |
PIRi | S56146 |
RefSeqi | NP_116664.1, NM_001179974.1 |
3D structure databases
SMRi | P43535 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 31159, 343 interactors |
ComplexPortali | CPX-1808, GCN1-GCN20 complex |
DIPi | DIP-2345N |
IntActi | P43535, 54 interactors |
MINTi | P43535 |
STRINGi | 4932.YFR009W |
PTM databases
iPTMneti | P43535 |
Proteomic databases
MaxQBi | P43535 |
PaxDbi | P43535 |
PRIDEi | P43535 |
Genome annotation databases
EnsemblFungii | YFR009W_mRNA; YFR009W; YFR009W |
GeneIDi | 850561 |
KEGGi | sce:YFR009W |
Organism-specific databases
SGDi | S000001905, GCN20 |
VEuPathDBi | FungiDB:YFR009W |
Phylogenomic databases
eggNOGi | KOG0062, Eukaryota |
GeneTreei | ENSGT00940000155604 |
HOGENOMi | CLU_000604_36_6_1 |
InParanoidi | P43535 |
OMAi | GNYTFWY |
Miscellaneous databases
PROi | PR:P43535 |
RNActi | P43535, protein |
Family and domain databases
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR032781, ABC_tran_Xtn IPR003439, ABC_transporter-like IPR017871, ABC_transporter_CS IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF00005, ABC_tran, 2 hits PF12848, ABC_tran_Xtn, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 2 hits |
SUPFAMi | SSF52540, SSF52540, 2 hits |
PROSITEi | View protein in PROSITE PS00211, ABC_TRANSPORTER_1, 1 hit PS50893, ABC_TRANSPORTER_2, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GCN20_YEAST | |
Accessioni | P43535Primary (citable) accession number: P43535 Secondary accession number(s): D6VTN9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | November 1, 1995 | |
Last modified: | February 10, 2021 | |
This is version 188 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - SIMILARITY comments
Index of protein domains and families - Yeast chromosome VI
Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names