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Protein

Caspase-1

Gene

Casp1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis. Upon inflammasome activation, during DNA virus infection but not RNA virus challenge, controls antiviral immunity through the cleavage of CGAS, rendering it inactive.By similarity

Catalytic activityi

Strict requirement for an Asp residue at position P1 and has a preferred cleavage sequence of Tyr-Val-Ala-Asp-|-.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei236By similarity1
Active sitei284By similarity1

GO - Molecular functioni

GO - Biological processi

  • apoptotic process Source: RGD
  • lung development Source: RGD
  • memory Source: RGD
  • microglial cell activation Source: RGD
  • midgut development Source: RGD
  • myoblast fusion Source: RGD
  • positive regulation of apoptotic process Source: RGD
  • positive regulation of circadian sleep/wake cycle, non-REM sleep Source: RGD
  • positive regulation of cytokine secretion Source: RGD
  • positive regulation of interleukin-1 beta secretion Source: RGD
  • regulation of inflammatory response Source: GO_Central
  • response to drug Source: RGD
  • response to hypoxia Source: RGD
  • response to lipopolysaccharide Source: RGD
  • response to organic cyclic compound Source: RGD
  • response to toxic substance Source: RGD

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease
Biological processApoptosis

Enzyme and pathway databases

BRENDAi3.4.22.36 5301

Protein family/group databases

MEROPSiC14.001

Names & Taxonomyi

Protein namesi
Recommended name:
Caspase-1 (EC:3.4.22.36)
Short name:
CASP-1
Alternative name(s):
Interleukin-1 beta convertase
Short name:
IL-1BC
Interleukin-1 beta-converting enzyme
Short name:
ICE
Short name:
IL-1 beta-converting enzyme
p45
Cleaved into the following 2 chains:
Gene namesi
Name:Casp1
Synonyms:Il1bc, Il1bce
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2274 Casp1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
PropeptideiPRO_00000045331 – ?118Sequence analysisAdd BLAST118
ChainiPRO_0000004534?119 – 296Caspase-1 subunit p20Add BLAST178
PropeptideiPRO_0000004535297 – 314Sequence analysisAdd BLAST18
ChainiPRO_0000004536315 – 402Caspase-1 subunit p10Add BLAST88

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei301PhosphoserineBy similarity1

Post-translational modificationi

The two subunits are derived from the precursor sequence by a autocatalytic mechanism.

Keywords - PTMi

Phosphoprotein, Zymogen

Proteomic databases

PaxDbiP43527
PRIDEiP43527

PTM databases

PhosphoSitePlusiP43527

Interactioni

Subunit structurei

Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed bya 20 kDa (p20) and a 10 kDa (p10) subunit. The p20 subunit can also form a heterodimer with the epsilon isoform which then has an inhibitory effect. May be a component of the inflammasome, a protein complex which also includes PYCARD, CARD8 and NALP2 and whose function would be the activation of proinflammatory caspases. Both the p20 and p10 subunits interact with MEFV. Interacts with CARD17/INCA and CARD18 (By similarity).By similarity

GO - Molecular functioni

  • scaffold protein binding Source: RGD

Protein-protein interaction databases

DIPiDIP-29840N
IntActiP43527, 1 interactor
STRINGi10116.ENSRNOP00000009993

Structurei

3D structure databases

ProteinModelPortaliP43527
SMRiP43527
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 91CARDPROSITE-ProRule annotationAdd BLAST91

Sequence similaritiesi

Belongs to the peptidase C14A family.Curated

Phylogenomic databases

eggNOGiKOG3573 Eukaryota
ENOG410ZQIE LUCA
HOGENOMiHOG000234399
HOVERGENiHBG076981
InParanoidiP43527
PhylomeDBiP43527

Family and domain databases

CDDicd00032 CASc, 1 hit
InterProiView protein in InterPro
IPR001315 CARD
IPR029030 Caspase-like_dom_sf
IPR033139 Caspase_cys_AS
IPR016129 Caspase_his_AS
IPR011029 DEATH-like_dom_sf
IPR002138 Pept_C14_p10
IPR001309 Pept_C14_p20
IPR015917 Pept_C14A
PfamiView protein in Pfam
PF00619 CARD, 1 hit
PRINTSiPR00376 IL1BCENZYME
SMARTiView protein in SMART
SM00114 CARD, 1 hit
SM00115 CASc, 1 hit
SUPFAMiSSF47986 SSF47986, 1 hit
SSF52129 SSF52129, 1 hit
PROSITEiView protein in PROSITE
PS50209 CARD, 1 hit
PS01122 CASPASE_CYS, 1 hit
PS01121 CASPASE_HIS, 1 hit
PS50207 CASPASE_P10, 1 hit
PS50208 CASPASE_P20, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P43527-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADKVLRAKR KQFINSVSVG TINGLLDELL EKRVLNQEEM DTIKLANITV
60 70 80 90 100
MEKARDLCDH VTKKGPRASQ MFITYICNED CYLAEILELQ SGPSAETVFV
110 120 130 140 150
TEDSKGGHPF SSETKEKLNK EGGAFPGPSG SLKFCPLEIA QKLWKENHSE
160 170 180 190 200
IYPIMKTPTR TRLALIICNT DFQHLSRRVG ADVDLREMKL LLQDLGYTVK
210 220 230 240 250
VKENLTALEM TKELKEFAAC PEHKTSDSTF LVFMSHGLQE GICGITYSNE
260 270 280 290 300
VADILKVDTI FQMMNTLKCP SLKDKPKVII IQACRGEKQG VVLLKDSVGN
310 320 330 340 350
SEEGFLTDAI FEDDGIKKAH IEKDFIAFCS STPDNVSWRH PVRGSLFIES
360 370 380 390 400
LIKHMKEYAW SCDLEDIFRK VRFSFEQPDS RLQMPTTERV TLTKRFYLFP

GH
Length:402
Mass (Da):45,576
Last modified:November 1, 1995 - v1
Checksum:iD2E8FA2BEA76FD40
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti252A → G in AAC52259 (PubMed:7588240).Curated1
Sequence conflicti252A → G in AAB35431 (PubMed:7588240).Curated1
Sequence conflicti256K → E in AAC52259 (PubMed:7588240).Curated1
Sequence conflicti256K → E in AAB35431 (PubMed:7588240).Curated1
Sequence conflicti260I → L in AAC52259 (PubMed:7588240).Curated1
Sequence conflicti260I → L in AAB35431 (PubMed:7588240).Curated1
Sequence conflicti262Q → H in AAC52259 (PubMed:7588240).Curated1
Sequence conflicti262Q → H in AAB35431 (PubMed:7588240).Curated1
Sequence conflicti343R → Q in AAC52259 (PubMed:7588240).Curated1
Sequence conflicti343R → Q in AAB35431 (PubMed:7588240).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14647 mRNA Translation: AAA85812.1
U34621 mRNA Translation: AAC52259.1
S79676 mRNA Translation: AAB35431.1
UniGeneiRn.37508

Genome annotation databases

UCSCiRGD:2274 rat

Similar proteinsi

Entry informationi

Entry nameiCASP1_RAT
AccessioniPrimary (citable) accession number: P43527
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: June 20, 2018
This is version 143 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

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