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Protein

Interleukin-12 subunit beta

Gene

Il12b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.By similarity
Associates with IL23A to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionCytokine

Enzyme and pathway databases

ReactomeiR-MMU-9020591 Interleukin-12 signaling
R-MMU-9020933 Interleukin-23 signaling

Names & Taxonomyi

Protein namesi
Recommended name:
Interleukin-12 subunit beta
Short name:
IL-12B
Alternative name(s):
Cytotoxic lymphocyte maturation factor 40 kDa subunit
Short name:
CLMF p40
IL-12 subunit p40
Gene namesi
Name:Il12b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:96540 Il12b

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2176814

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22By similarityAdd BLAST22
ChainiPRO_000001093323 – 335Interleukin-12 subunit betaAdd BLAST313

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi47N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi50 ↔ 90PROSITE-ProRule annotation
Glycosylationi122N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi132N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi197Interchain (with C-92 in IL12A)PROSITE-ProRule annotation
Glycosylationi220N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP43432
PRIDEiP43432

PTM databases

iPTMnetiP43432
PhosphoSitePlusiP43432

Expressioni

Gene expression databases

BgeeiENSMUSG00000004296 Expressed in 28 organ(s), highest expression level in lumbar subsegment of spinal cord
CleanExiMM_IL12B
ExpressionAtlasiP43432 baseline and differential
GenevisibleiP43432 MM

Interactioni

Subunit structurei

Heterodimer with IL12A; disulfide-linked. The heterodimer is known as interleukin IL-12. Heterodimer with IL23A; disulfide-linked. The heterodimer is known as interleukin IL-23. Also secreted as a monomer.

Binary interactionsi

WithEntry#Exp.IntActNotes
Il23aQ9EQ143EBI-2481353,EBI-2481329

GO - Molecular functioni

Protein-protein interaction databases

ComplexPortaliCPX-3293 Interleukin-23 complex
CPX-387 Interleukin-12 complex
CPX-388 Interleukin-12-receptor complex
CPX-389 Interleukin-23-receptor complex
DIPiDIP-6013N
IntActiP43432, 1 interactor
STRINGi10090.ENSMUSP00000099860

Structurei

3D structure databases

ProteinModelPortaliP43432
SMRiP43432
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini23 – 106Ig-like C2-typeAdd BLAST84
Domaini233 – 324Fibronectin type-IIIAdd BLAST92

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi155 – 159Poly-Ser5

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain, Signal

Phylogenomic databases

eggNOGiENOG410IF5K Eukaryota
ENOG410YWXR LUCA
GeneTreeiENSGT00390000012630
HOGENOMiHOG000113027
HOVERGENiHBG052094
InParanoidiP43432
KOiK05425
OMAiCEAKNYS
OrthoDBiEOG091G0EKV
PhylomeDBiP43432
TreeFamiTF334829

Family and domain databases

CDDicd00063 FN3, 1 hit
Gene3Di2.60.40.10, 3 hits
InterProiView protein in InterPro
IPR003961 FN3_dom
IPR036116 FN3_sf
IPR003530 Hematopoietin_rcpt_L_F3_CS
IPR007110 Ig-like_dom
IPR036179 Ig-like_dom_sf
IPR013783 Ig-like_fold
IPR003598 Ig_sub2
IPR015528 IL-12_beta
IPR019482 IL-12_beta_cen-dom
PANTHERiPTHR44982 PTHR44982, 1 hit
PfamiView protein in Pfam
PF10420 IL12p40_C, 1 hit
PIRSFiPIRSF038007 IL_12_beta, 1 hit
PRINTSiPR01928 INTRLEUKN12B
SMARTiView protein in SMART
SM00408 IGc2, 1 hit
SUPFAMiSSF48726 SSF48726, 1 hit
SSF49265 SSF49265, 2 hits
PROSITEiView protein in PROSITE
PS01354 HEMATOPO_REC_L_F3, 1 hit
PS50835 IG_LIKE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P43432-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MCPQKLTISW FAIVLLVSPL MAMWELEKDV YVVEVDWTPD APGETVNLTC
60 70 80 90 100
DTPEEDDITW TSDQRHGVIG SGKTLTITVK EFLDAGQYTC HKGGETLSHS
110 120 130 140 150
HLLLHKKENG IWSTEILKNF KNKTFLKCEA PNYSGRFTCS WLVQRNMDLK
160 170 180 190 200
FNIKSSSSSP DSRAVTCGMA SLSAEKVTLD QRDYEKYSVS CQEDVTCPTA
210 220 230 240 250
EETLPIELAL EARQQNKYEN YSTSFFIRDI IKPDPPKNLQ MKPLKNSQVE
260 270 280 290 300
VSWEYPDSWS TPHSYFSLKF FVRIQRKKEK MKETEEGCNQ KGAFLVEKTS
310 320 330
TEVQCKGGNV CVQAQDRYYN SSCSKWACVP CRVRS
Length:335
Mass (Da):38,235
Last modified:November 1, 1995 - v1
Checksum:i19E4FA5EF0CFC82A
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti169M → T in strain: B10.S/J and SJL/J. 1 Publication1
Natural varianti294F → L in strain: B10.S/J and SJL/J. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M86671 mRNA Translation: AAA39296.1
S82426
, S82421, S82422, S82424, S82425 Genomic DNA Translation: AAB37383.1
AF128214 mRNA Translation: AAF22555.1
AF128215 mRNA Translation: AAF22556.1
CCDSiCCDS24563.1
PIRiI72789
RefSeqiNP_001290173.1, NM_001303244.1
UniGeneiMm.239707

Genome annotation databases

EnsembliENSMUST00000102796; ENSMUSP00000099860; ENSMUSG00000004296
ENSMUST00000170513; ENSMUSP00000125867; ENSMUSG00000004296
GeneIDi16160
KEGGimmu:16160
UCSCiuc007inc.2 mouse

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M86671 mRNA Translation: AAA39296.1
S82426
, S82421, S82422, S82424, S82425 Genomic DNA Translation: AAB37383.1
AF128214 mRNA Translation: AAF22555.1
AF128215 mRNA Translation: AAF22556.1
CCDSiCCDS24563.1
PIRiI72789
RefSeqiNP_001290173.1, NM_001303244.1
UniGeneiMm.239707

3D structure databases

ProteinModelPortaliP43432
SMRiP43432
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

ComplexPortaliCPX-3293 Interleukin-23 complex
CPX-387 Interleukin-12 complex
CPX-388 Interleukin-12-receptor complex
CPX-389 Interleukin-23-receptor complex
DIPiDIP-6013N
IntActiP43432, 1 interactor
STRINGi10090.ENSMUSP00000099860

Chemistry databases

ChEMBLiCHEMBL2176814

PTM databases

iPTMnetiP43432
PhosphoSitePlusiP43432

Proteomic databases

PaxDbiP43432
PRIDEiP43432

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000102796; ENSMUSP00000099860; ENSMUSG00000004296
ENSMUST00000170513; ENSMUSP00000125867; ENSMUSG00000004296
GeneIDi16160
KEGGimmu:16160
UCSCiuc007inc.2 mouse

Organism-specific databases

CTDi3593
MGIiMGI:96540 Il12b

Phylogenomic databases

eggNOGiENOG410IF5K Eukaryota
ENOG410YWXR LUCA
GeneTreeiENSGT00390000012630
HOGENOMiHOG000113027
HOVERGENiHBG052094
InParanoidiP43432
KOiK05425
OMAiCEAKNYS
OrthoDBiEOG091G0EKV
PhylomeDBiP43432
TreeFamiTF334829

Enzyme and pathway databases

ReactomeiR-MMU-9020591 Interleukin-12 signaling
R-MMU-9020933 Interleukin-23 signaling

Miscellaneous databases

PROiPR:P43432
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000004296 Expressed in 28 organ(s), highest expression level in lumbar subsegment of spinal cord
CleanExiMM_IL12B
ExpressionAtlasiP43432 baseline and differential
GenevisibleiP43432 MM

Family and domain databases

CDDicd00063 FN3, 1 hit
Gene3Di2.60.40.10, 3 hits
InterProiView protein in InterPro
IPR003961 FN3_dom
IPR036116 FN3_sf
IPR003530 Hematopoietin_rcpt_L_F3_CS
IPR007110 Ig-like_dom
IPR036179 Ig-like_dom_sf
IPR013783 Ig-like_fold
IPR003598 Ig_sub2
IPR015528 IL-12_beta
IPR019482 IL-12_beta_cen-dom
PANTHERiPTHR44982 PTHR44982, 1 hit
PfamiView protein in Pfam
PF10420 IL12p40_C, 1 hit
PIRSFiPIRSF038007 IL_12_beta, 1 hit
PRINTSiPR01928 INTRLEUKN12B
SMARTiView protein in SMART
SM00408 IGc2, 1 hit
SUPFAMiSSF48726 SSF48726, 1 hit
SSF49265 SSF49265, 2 hits
PROSITEiView protein in PROSITE
PS01354 HEMATOPO_REC_L_F3, 1 hit
PS50835 IG_LIKE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiIL12B_MOUSE
AccessioniPrimary (citable) accession number: P43432
Secondary accession number(s): Q9QUM1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 7, 2018
This is version 163 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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