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Protein

Interleukin-12 subunit alpha

Gene

Il12a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionCytokine, Growth factor

Enzyme and pathway databases

ReactomeiR-MMU-8984722 Interleukin-35 Signalling
R-MMU-9020591 Interleukin-12 signaling

Names & Taxonomyi

Protein namesi
Recommended name:
Interleukin-12 subunit alpha
Short name:
IL-12A
Alternative name(s):
Cytotoxic lymphocyte maturation factor 35 kDa subunit
Short name:
CLMF p35
IL-12 subunit p35
Gene namesi
Name:Il12a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:96539 Il12a

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22By similarityAdd BLAST22
ChainiPRO_000001560823 – 215Interleukin-12 subunit alphaAdd BLAST193

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi60 ↔ 192By similarity
Disulfide bondi81 ↔ 119By similarity
Glycosylationi89N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi92Interchain (with C-197 in IL12B)By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP43431
PRIDEiP43431

PTM databases

PhosphoSitePlusiP43431

Expressioni

Gene expression databases

BgeeiENSMUSG00000027776 Expressed in 47 organ(s), highest expression level in bone marrow
CleanExiMM_IL12A
ExpressionAtlasiP43431 baseline and differential
GenevisibleiP43431 MM

Interactioni

Subunit structurei

Heterodimer with IL12B; disulfide-linked (PubMed:1350290). This heterodimer is known as interleukin IL-12 (PubMed:1350290). Heterodimer with EBI3/IL27B; not disulfide-linked (By similarity). This heterodimer is known as interleukin IL-35 (By similarity).By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Ebi3O352282EBI-3862959,EBI-3862967

GO - Molecular functioni

Protein-protein interaction databases

ComplexPortaliCPX-387 Interleukin-12 complex
CPX-388 Interleukin-12-receptor complex
DIPiDIP-60363N
IntActiP43431, 1 interactor
STRINGi10090.ENSMUSP00000029345

Structurei

3D structure databases

ProteinModelPortaliP43431
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the IL-6 superfamily.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IVIR Eukaryota
ENOG410ZGHJ LUCA
GeneTreeiENSGT00390000016906
HOGENOMiHOG000113026
HOVERGENiHBG063691
InParanoidiP43431
KOiK05406
PhylomeDBiP43431

Family and domain databases

InterProiView protein in InterPro
IPR009079 4_helix_cytokine-like_core
IPR004281 IL-12_alpha
PANTHERiPTHR45311 PTHR45311, 1 hit
PfamiView protein in Pfam
PF03039 IL12, 1 hit
SUPFAMiSSF47266 SSF47266, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

P43431-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MCQSRYLLFL ATLALLNHLS LARVIPVSGP ARCLSQSRNL LKTTDDMVKT
60 70 80 90 100
AREKLKHYSC TAEDIDHEDI TRDQTSTLKT CLPLELHKNE SCLATRETSS
110 120 130 140 150
TTRGSCLPPQ KTSLMMTLCL GSIYEDLKMY QTEFQAINAA LQNHNHQQII
160 170 180 190 200
LDKGMLVAID ELMQSLNHNG ETLRQKPPVG EADPYRVKMK LCILLHAFST
210
RVVTINRVMG YLSSA
Length:215
Mass (Da):24,179
Last modified:November 1, 1995 - v1
Checksum:i56845B9B66D35A53
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F8WI71F8WI71_MOUSE
Interleukin-12 subunit alpha
Il12a IL12A
236Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M86672 mRNA Translation: AAA39292.1
S82419
, S82412, S82414, S82417, S82418 Genomic DNA Translation: AAB37382.1
D63334 Genomic DNA Translation: BAA09647.1
CCDSiCCDS17400.1
PIRiI56135
RefSeqiNP_001152896.1, NM_001159424.2
NP_032377.1, NM_008351.3
UniGeneiMm.103783

Genome annotation databases

EnsembliENSMUST00000107816; ENSMUSP00000103446; ENSMUSG00000027776
GeneIDi16159
KEGGimmu:16159
UCSCiuc008plv.3 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M86672 mRNA Translation: AAA39292.1
S82419
, S82412, S82414, S82417, S82418 Genomic DNA Translation: AAB37382.1
D63334 Genomic DNA Translation: BAA09647.1
CCDSiCCDS17400.1
PIRiI56135
RefSeqiNP_001152896.1, NM_001159424.2
NP_032377.1, NM_008351.3
UniGeneiMm.103783

3D structure databases

ProteinModelPortaliP43431
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

ComplexPortaliCPX-387 Interleukin-12 complex
CPX-388 Interleukin-12-receptor complex
DIPiDIP-60363N
IntActiP43431, 1 interactor
STRINGi10090.ENSMUSP00000029345

PTM databases

PhosphoSitePlusiP43431

Proteomic databases

PaxDbiP43431
PRIDEiP43431

Protocols and materials databases

DNASUi16159
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000107816; ENSMUSP00000103446; ENSMUSG00000027776
GeneIDi16159
KEGGimmu:16159
UCSCiuc008plv.3 mouse

Organism-specific databases

CTDi3592
MGIiMGI:96539 Il12a

Phylogenomic databases

eggNOGiENOG410IVIR Eukaryota
ENOG410ZGHJ LUCA
GeneTreeiENSGT00390000016906
HOGENOMiHOG000113026
HOVERGENiHBG063691
InParanoidiP43431
KOiK05406
PhylomeDBiP43431

Enzyme and pathway databases

ReactomeiR-MMU-8984722 Interleukin-35 Signalling
R-MMU-9020591 Interleukin-12 signaling

Miscellaneous databases

PROiPR:P43431
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027776 Expressed in 47 organ(s), highest expression level in bone marrow
CleanExiMM_IL12A
ExpressionAtlasiP43431 baseline and differential
GenevisibleiP43431 MM

Family and domain databases

InterProiView protein in InterPro
IPR009079 4_helix_cytokine-like_core
IPR004281 IL-12_alpha
PANTHERiPTHR45311 PTHR45311, 1 hit
PfamiView protein in Pfam
PF03039 IL12, 1 hit
SUPFAMiSSF47266 SSF47266, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiIL12A_MOUSE
AccessioniPrimary (citable) accession number: P43431
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 7, 2018
This is version 140 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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