Gata1

Functionⁱ

Transcriptional activator or repressor which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence 5'-[AT]GATA[AG]-3' within regulatory regions of globin genes and of other genes expressed in erythroid cells. Activates the transcription of genes involved in erythroid differentiation of K562 erythroleukemia cells, including HBB, HBG1/2, ALAS2 and HMBS.By similarity

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Zinc fingerⁱ	204 – 228	GATA-type 1PROSITE-ProRule annotationAdd BLAST		25
Zinc fingerⁱ	258 – 282	GATA-type 2PROSITE-ProRule annotationAdd BLAST		25

GO - Molecular functionⁱ

chromatin binding
Source: RGD
Inferred from direct assayⁱ
- 22885997
chromatin DNA binding Source: UniProtKB
DNA binding
Source: RGD
Inferred from direct assayⁱ
- 7957872
RNA polymerase II distal enhancer sequence-specific DNA binding Source: UniProtKB
RNA polymerase II regulatory region sequence-specific DNA binding Source: GO_Central
RNA polymerase II transcription factor binding Source: GO_Central
sequence-specific DNA binding
Source: RGD
Inferred from direct assayⁱ
- 7957872
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: GO_Central
zinc ion binding Source: InterPro

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

animal organ regeneration
Source: RGD
Inferred from direct assayⁱ
- 18990226
basophil differentiation Source: InterPro
cellular response to cAMP
Source: RGD
Inferred from expression patternⁱ
- 11861504
cellular response to follicle-stimulating hormone stimulus
Source: RGD
Inferred from expression patternⁱ
- 11861504
cellular response to lipopolysaccharide
Source: RGD
Inferred from direct assayⁱ
- 17687519
eosinophil differentiation Source: InterPro
erythrocyte development Source: InterPro
megakaryocyte differentiation Source: InterPro
negative regulation of transcription by RNA polymerase II Source: UniProtKB
positive regulation of cytosolic calcium ion concentration
Source: RGD
Inferred from mutant phenotypeⁱ
- 22390417
positive regulation of mast cell degranulation
Source: RGD
Inferred from mutant phenotypeⁱ
- 22390417
positive regulation of transcription, DNA-templated Source: UniProtKB
regulation of transcription, DNA-templated
Source: RGD
Traceable author statementⁱ
- 7957872
Sertoli cell development
Source: RGD
Inferred from expression patternⁱ
- 9089398

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Activator, DNA-binding, Repressor
Biological process	Transcription, Transcription regulation
Ligand	Metal-binding, Zinc

Enzyme and pathway databases

Reactomeⁱ	R-RNO-8936459 RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function R-RNO-8939236 RUNX1 regulates transcription of genes involved in differentiation of HSCs R-RNO-983231 Factors involved in megakaryocyte development and platelet production

Reactomeⁱ

R-RNO-8936459 RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function
R-RNO-8939236 RUNX1 regulates transcription of genes involved in differentiation of HSCs
R-RNO-983231 Factors involved in megakaryocyte development and platelet production

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Erythroid transcription factor Alternative name(s): Eryf1 GATA-binding factor 1 Short name: GATA-1 Short name: GF-1 NF-E1 DNA-binding protein
Gene namesⁱ	Name:Gata1
Organismⁱ	Rattus norvegicus (Rat)
Taxonomic identifierⁱ	10116 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus
Proteomesⁱ	UP000002494 Componentⁱ: Chromosome X

Organism-specific databases

Rat genome database More...RGDⁱ	2663 Gata1

RGDⁱ

2663 Gata1

Keywords - Cellular componentⁱ

Nucleus

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000083399	1 – 413	Erythroid transcription factorAdd BLAST		413

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	26	PhosphoserineBy similarity	1
Modified residueⁱ	49	PhosphoserineBy similarity	1
Modified residueⁱ	72	PhosphoserineBy similarity	1
Cross-linkⁱ	137	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Modified residueⁱ	142	PhosphoserineBy similarity	1
Modified residueⁱ	178	PhosphoserineBy similarity	1
Modified residueⁱ	187	PhosphoserineBy similarity	1
Modified residueⁱ	233	N6-acetyllysine; by EP300By similarity	1
Modified residueⁱ	245	N6-acetyllysine; by EP300By similarity	1
Modified residueⁱ	246	N6-acetyllysine; by CREBBPBy similarity	1
Modified residueⁱ	246	N6-acetyllysine; by EP300By similarity	1
Modified residueⁱ	252	N6-acetyllysine; by CREBBPBy similarity	1
Modified residueⁱ	308	N6-acetyllysineBy similarity	1
Modified residueⁱ	310	PhosphoserineBy similarity	1
Modified residueⁱ	312	N6-acetyllysine; by CREBBPBy similarity	1
Modified residueⁱ	314	N6-acetyllysineBy similarity	1
Modified residueⁱ	315	N6-acetyllysineBy similarity	1

Post-translational modificationⁱ

Highly phosphorylated on serine residues. Phosphorylation on Ser-310 is enhanced on erythroid differentiation. Phosphorylation on Ser-142 promotes sumoylation on Lys-137 (By similarity).By similarity

Sumoylation on Lys-137 is enhanced by phosphorylation on Ser-142 and by interaction with PIAS4. Sumoylation with SUMO1 has no effect on transcriptional activity (By similarity).By similarity

Acetylated at 2 conserved lysine-rich motifs by CREBBP in vitro. Acetylation does not affect DNA-binding in vitro but is essential to induce erythroid differentiation and for binding chromatin in vivo. Acetylated on Lys-233, Lys-245 Lys-246 by EP300 (By similarity).By similarity

Keywords - PTMⁱ

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbⁱ	P43429
PRIDEⁱ	P43429

PTM databases

PhosphoSitePlusⁱ	P43429

PhosphoSitePlusⁱ

P43429

Expressionⁱ

Tissue specificityⁱ

Erythrocytes, and fetal hepatocytes.1 Publication

Gene expression databases

Bgeeⁱ	ENSRNOG00000025534 Expressed in 4 organ(s), highest expression level in spleen
Genevisibleⁱ	P43429 RN

Interactionⁱ

Subunit structureⁱ

Interacts (via the N-terminal zinc finger) with ZFPM1. Interacts with GFI1B. Interacts with PIAS4; the interaction enhances sumoylation and represses the transactivational activity in a sumoylation-independent manner. Interacts with LMCD1. Interacts with BRD3 (By similarity). Interacts with CREBBP; the interaction stimulates acetylation and transcriptional activity in vivo. Interacts with MED1, CCAR1 and CALCOCO1 (By similarity). Interacts with EP300 (By similarity). Interacts with CEBPE (By similarity).By similarity

GO - Molecular functionⁱ

RNA polymerase II transcription factor binding Source: GO_Central

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

STRINGⁱ	10116.ENSRNOP00000067336

STRINGⁱ

10116.ENSRNOP00000067336

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P43429
SMRⁱ	P43429
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Length
Regionⁱ	200 – 330	Interaction with MED1 and CCAR1By similarityAdd BLAST	131
Regionⁱ	203 – 222	Required for interaction with ZFPM1By similarityAdd BLAST	20
Regionⁱ	249 – 315	Interaction with CALCOCO1By similarityAdd BLAST	67

Domainⁱ

The two fingers are functionally distinct and cooperate to achieve specific, stable DNA binding. The first finger is necessary only for full specificity and stability of binding, whereas the second one is required for binding (By similarity).By similarity

Zinc finger

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Zinc fingerⁱ	204 – 228	GATA-type 1PROSITE-ProRule annotationAdd BLAST		25
Zinc fingerⁱ	258 – 282	GATA-type 2PROSITE-ProRule annotationAdd BLAST		25

Keywords - Domainⁱ

Repeat, Zinc-finger

Phylogenomic databases

eggNOGⁱ	KOG1601 Eukaryota COG5641 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00760000119221
HOGENOMⁱ	HOG000047701
HOVERGENⁱ	HBG051705
InParanoidⁱ	P43429
KEGG Orthology (KO) More...KOⁱ	K09182
OMAⁱ	TLKTERM
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0AUR
PhylomeDBⁱ	P43429
TreeFamⁱ	TF315391

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00202 ZnF_GATA, 2 hits
Gene3Dⁱ	3.30.50.10, 2 hits
InterProⁱ	View protein in InterPro IPR029524 GATA-1 IPR039355 Transcription_factor_GATA IPR000679 Znf_GATA IPR013088 Znf_NHR/GATA
PANTHERⁱ	PTHR10071 PTHR10071, 1 hit PTHR10071:SF190 PTHR10071:SF190, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00320 GATA, 2 hits
PRINTSⁱ	PR00619 GATAZNFINGER
SMARTⁱ	View protein in SMART SM00401 ZnF_GATA, 2 hits
PROSITEⁱ	View protein in PROSITE PS00344 GATA_ZN_FINGER_1, 2 hits PS50114 GATA_ZN_FINGER_2, 2 hits

Sequenceⁱ

Sequence statusⁱ: Complete.

P43429-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MDFPGLGALG TSEPLPQFVD SALVSSTSDS AGFFSSGPES LDTASSSTSP 
        60         70         80         90        100
NAATAAATAL AYYREAEAYR HSPVFQVYPL LNSMEGIPGS SPYASWAYSK 
       110        120        130        140        150
TALYPASTVC PSHEDAPSQT LEDPDGKNNN TFLETLKTER LSPDLLTLGT 
       160        170        180        190        200
ALPTSLPVTS SAYGGADFPS PFFSPTGSPL SSAAYSSPKF HGSLPLAPCE 
       210        220        230        240        250
ARECVNCGAT ATPLWRRDRT GHYLCNACGL YHKMNGQNRP LIRPKKRMIV 
       260        270        280        290        300
SKRAGTQCTN CQTTTTTLWR RNASGDPVCN ACGLYYKLHQ VNRPLTMRKD 
       310        320        330        340        350
GIQTRNRKAS GKGKKKRGSS LAGAGAAEGP AGGFMVVAGG SSSGNCGEVA 
       360        370        380        390        400
PGLTLGTAGT AHLYQGLGPV VLSGPVSHLM SFPGPLLGSP TASFPTGPVP 
       410 
TTTSTSVVSP LSS

Length:413

Mass (Da):42,871

Last modified:November 1, 1995 - v1

Checksum:ⁱ9BE4120FCF8269BB

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	D13518 mRNA Translation: BAA02735.1
PIRⁱ	S48756
NCBI Reference Sequences More...RefSeqⁱ	NP_036896.1, NM_012764.1 XP_017457744.1, XM_017602255.1
UniGeneⁱ	Rn.10024

Genome annotation databases

Ensemblⁱ	ENSRNOT00000029272; ENSRNOP00000038111; ENSRNOG00000025534 ENSRNOT00000075082; ENSRNOP00000067336; ENSRNOG00000047663
GeneIDⁱ	108348091 25172
KEGGⁱ	rno:108348091 rno:25172
UCSC genome browser More...UCSCⁱ	RGD:2663 rat

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
P43429	erythroid transcription factor		MESAU		413	UniRef90_P43429
Erythroid transcription factor		CRIGR		330
UPI000B4ED315		MERUN		419
UPI000A308C90		MUSPA		413
UPI000A315665		MUSCR		413
+3

Protein	Similar proteins		Species	Score	Length	Source
P43429	Erythroid transcription factor		HUMAN		413	UniRef50_P15976
Erythroid transcription factor	)	MOUSE		413
Erythroid transcription factor		CASCN		432
Uncharacterized protein		RHIBE		421
Uncharacterized protein		RHIRO		413
+140

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	D13518 mRNA Translation: BAA02735.1
PIRⁱ	S48756
RefSeqⁱ	NP_036896.1, NM_012764.1 XP_017457744.1, XM_017602255.1
UniGeneⁱ	Rn.10024

3D structure databases

ProteinModelPortalⁱ	P43429
SMRⁱ	P43429
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

STRINGⁱ	10116.ENSRNOP00000067336

STRINGⁱ

10116.ENSRNOP00000067336

PTM databases

PhosphoSitePlusⁱ	P43429

PhosphoSitePlusⁱ

P43429

Proteomic databases

PaxDbⁱ	P43429
PRIDEⁱ	P43429

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSRNOT00000029272; ENSRNOP00000038111; ENSRNOG00000025534 ENSRNOT00000075082; ENSRNOP00000067336; ENSRNOG00000047663
GeneIDⁱ	108348091 25172
KEGGⁱ	rno:108348091 rno:25172
UCSCⁱ	RGD:2663 rat

Organism-specific databases

CTDⁱ	2623
RGDⁱ	2663 Gata1

Phylogenomic databases

eggNOGⁱ	KOG1601 Eukaryota COG5641 LUCA
GeneTreeⁱ	ENSGT00760000119221
HOGENOMⁱ	HOG000047701
HOVERGENⁱ	HBG051705
InParanoidⁱ	P43429
KOⁱ	K09182
OMAⁱ	TLKTERM
OrthoDBⁱ	EOG091G0AUR
PhylomeDBⁱ	P43429
TreeFamⁱ	TF315391

Enzyme and pathway databases

Reactomeⁱ	R-RNO-8936459 RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function R-RNO-8939236 RUNX1 regulates transcription of genes involved in differentiation of HSCs R-RNO-983231 Factors involved in megakaryocyte development and platelet production

Reactomeⁱ

R-RNO-8936459 RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function
R-RNO-8939236 RUNX1 regulates transcription of genes involved in differentiation of HSCs
R-RNO-983231 Factors involved in megakaryocyte development and platelet production

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:P43429

PROⁱ

PR:P43429

Gene expression databases

Bgeeⁱ	ENSRNOG00000025534 Expressed in 4 organ(s), highest expression level in spleen
Genevisibleⁱ	P43429 RN

Family and domain databases

CDDⁱ	cd00202 ZnF_GATA, 2 hits
Gene3Dⁱ	3.30.50.10, 2 hits
InterProⁱ	View protein in InterPro IPR029524 GATA-1 IPR039355 Transcription_factor_GATA IPR000679 Znf_GATA IPR013088 Znf_NHR/GATA
PANTHERⁱ	PTHR10071 PTHR10071, 1 hit PTHR10071:SF190 PTHR10071:SF190, 1 hit
Pfamⁱ	View protein in Pfam PF00320 GATA, 2 hits
PRINTSⁱ	PR00619 GATAZNFINGER
SMARTⁱ	View protein in SMART SM00401 ZnF_GATA, 2 hits
PROSITEⁱ	View protein in PROSITE PS00344 GATA_ZN_FINGER_1, 2 hits PS50114 GATA_ZN_FINGER_2, 2 hits
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	GATA1_RAT
Accessionⁱ	P43429Primary (citable) accession number: P43429
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
	Last sequence update:	November 1, 1995
	Last modified:	September 12, 2018
	This is version 146 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P43429 (GATA1_RAT)

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Erythroid transcription factor

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Nucleus

Other locations

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

Zinc finger

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ