UniProtKB - P43429 (GATA1_RAT)
Erythroid transcription factor
Gata1
Functioni
Transcriptional activator or repressor which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence 5'-[AT]GATA[AG]-3' within regulatory regions of globin genes and of other genes expressed in erythroid cells. Activates the transcription of genes involved in erythroid differentiation of K562 erythroleukemia cells, including HBB, HBG1/2, ALAS2 and HMBS.
By similarityRegions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 204 – 228 | GATA-type 1PROSITE-ProRule annotationAdd BLAST | 25 | |
Zinc fingeri | 258 – 282 | GATA-type 2PROSITE-ProRule annotationAdd BLAST | 25 |
GO - Molecular functioni
- C2H2 zinc finger domain binding Source: RGD
- chromatin binding Source: RGD
- chromatin DNA binding Source: UniProtKB
- cis-regulatory region sequence-specific DNA binding Source: RGD
- DNA binding Source: RGD
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: RGD
- DNA-binding transcription factor activity Source: RGD
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
- p53 binding Source: RGD
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: UniProtKB
- RNA polymerase II-specific DNA-binding transcription factor binding Source: RGD
- RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: RGD
- sequence-specific DNA binding Source: RGD
- sequence-specific double-stranded DNA binding Source: RGD
- transcription cis-regulatory region binding Source: RGD
- transcription coactivator binding Source: Ensembl
- zinc ion binding Source: InterPro
GO - Biological processi
- animal organ regeneration Source: RGD
- basophil differentiation Source: RGD
- cell-cell signaling Source: RGD
- cell development Source: RGD
- cell fate commitment Source: GO_Central
- cellular response to cAMP Source: RGD
- cellular response to follicle-stimulating hormone stimulus Source: RGD
- cellular response to lipopolysaccharide Source: RGD
- cellular response to thyroid hormone stimulus Source: RGD
- dendritic cell differentiation Source: RGD
- embryonic hemopoiesis Source: RGD
- eosinophil differentiation Source: RGD
- eosinophil fate commitment Source: RGD
- erythrocyte development Source: RGD
- erythrocyte differentiation Source: RGD
- homeostasis of number of cells within a tissue Source: RGD
- in utero embryonic development Source: RGD
- male gonad development Source: RGD
- megakaryocyte differentiation Source: RGD
- myeloid cell differentiation Source: RGD
- negative regulation of apoptotic process Source: RGD
- negative regulation of bone mineralization Source: RGD
- negative regulation of cell population proliferation Source: RGD
- negative regulation of extrinsic apoptotic signaling pathway in absence of ligand Source: RGD
- negative regulation of transcription by RNA polymerase II Source: UniProtKB
- negative regulation of transcription regulatory region DNA binding Source: RGD
- platelet aggregation Source: RGD
- platelet formation Source: RGD
- positive regulation of cytosolic calcium ion concentration Source: RGD
- positive regulation of erythrocyte differentiation Source: RGD
- positive regulation of mast cell degranulation Source: RGD
- positive regulation of osteoblast proliferation Source: RGD
- positive regulation of peptidyl-tyrosine phosphorylation Source: RGD
- positive regulation of transcription, DNA-templated Source: UniProtKB
- positive regulation of transcription by RNA polymerase II Source: RGD
- regulation of definitive erythrocyte differentiation Source: RGD
- regulation of glycoprotein biosynthetic process Source: RGD
- regulation of primitive erythrocyte differentiation Source: RGD
- regulation of transcription, DNA-templated Source: RGD
- Sertoli cell development Source: RGD
Keywordsi
Molecular function | Activator, DNA-binding, Repressor |
Biological process | Transcription, Transcription regulation |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-RNO-8936459, RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function R-RNO-8939236, RUNX1 regulates transcription of genes involved in differentiation of HSCs R-RNO-983231, Factors involved in megakaryocyte development and platelet production |
Names & Taxonomyi
Protein namesi | Recommended name: Erythroid transcription factorAlternative name(s): Eryf1 GATA-binding factor 1 Short name: GATA-1 Short name: GF-1 NF-E1 DNA-binding protein |
Gene namesi | Name:Gata1 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 2663, Gata1 |
Subcellular locationi
Nucleus
Nucleus
- nucleoplasm Source: Ensembl
- nucleus Source: RGD
Other locations
- protein-DNA complex Source: RGD
- transcription regulator complex Source: RGD
- transcription repressor complex Source: RGD
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000083399 | 1 – 413 | Erythroid transcription factorAdd BLAST | 413 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 26 | PhosphoserineBy similarity | 1 | |
Modified residuei | 49 | PhosphoserineBy similarity | 1 | |
Modified residuei | 72 | PhosphoserineBy similarity | 1 | |
Cross-linki | 137 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity | ||
Modified residuei | 142 | PhosphoserineBy similarity | 1 | |
Modified residuei | 178 | PhosphoserineBy similarity | 1 | |
Modified residuei | 187 | PhosphoserineBy similarity | 1 | |
Modified residuei | 233 | N6-acetyllysine; by EP300By similarity | 1 | |
Modified residuei | 245 | N6-acetyllysine; by EP300By similarity | 1 | |
Modified residuei | 246 | N6-acetyllysine; by CREBBPBy similarity | 1 | |
Modified residuei | 246 | N6-acetyllysine; by EP300By similarity | 1 | |
Modified residuei | 252 | N6-acetyllysine; by CREBBPBy similarity | 1 | |
Modified residuei | 308 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 310 | PhosphoserineBy similarity | 1 | |
Modified residuei | 312 | N6-acetyllysine; by CREBBPBy similarity | 1 | |
Modified residuei | 314 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 315 | N6-acetyllysineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
PaxDbi | P43429 |
PTM databases
PhosphoSitePlusi | P43429 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSRNOG00000025534, Expressed in spleen and 14 other tissues |
Genevisiblei | P43429, RN |
Interactioni
Subunit structurei
Interacts (via the N-terminal zinc finger) with ZFPM1.
Interacts with GFI1B.
Interacts with PIAS4; the interaction enhances sumoylation and represses the transactivational activity in a sumoylation-independent manner.
Interacts with LMCD1.
Interacts with BRD3 (By similarity).
Interacts with CREBBP; the interaction stimulates acetylation and transcriptional activity in vivo.
Interacts with MED1, CCAR1 and CALCOCO1 (By similarity).
Interacts with EP300 (By similarity).
Interacts with CEBPE (By similarity).
By similarityGO - Molecular functioni
- C2H2 zinc finger domain binding Source: RGD
- p53 binding Source: RGD
- RNA polymerase II-specific DNA-binding transcription factor binding Source: RGD
- transcription coactivator binding Source: Ensembl
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000038111 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 109 – 128 | DisorderedSequence analysisAdd BLAST | 20 | |
Regioni | 200 – 330 | Interaction with MED1 and CCAR1By similarityAdd BLAST | 131 | |
Regioni | 203 – 222 | Required for interaction with ZFPM1By similarityAdd BLAST | 20 | |
Regioni | 249 – 315 | Interaction with CALCOCO1By similarityAdd BLAST | 67 | |
Regioni | 297 – 325 | DisorderedSequence analysisAdd BLAST | 29 | |
Regioni | 391 – 413 | DisorderedSequence analysisAdd BLAST | 23 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 394 – 413 | Polar residuesSequence analysisAdd BLAST | 20 |
Domaini
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 204 – 228 | GATA-type 1PROSITE-ProRule annotationAdd BLAST | 25 | |
Zinc fingeri | 258 – 282 | GATA-type 2PROSITE-ProRule annotationAdd BLAST | 25 |
Keywords - Domaini
Repeat, Zinc-fingerPhylogenomic databases
eggNOGi | KOG1601, Eukaryota |
GeneTreei | ENSGT00940000161156 |
HOGENOMi | CLU_027524_1_1_1 |
InParanoidi | P43429 |
OMAi | YSKTGLY |
OrthoDBi | 807790at2759 |
PhylomeDBi | P43429 |
TreeFami | TF315391 |
Family and domain databases
CDDi | cd00202, ZnF_GATA, 2 hits |
Gene3Di | 3.30.50.10, 2 hits |
InterProi | View protein in InterPro IPR029524, GATA-1 IPR039355, Transcription_factor_GATA IPR000679, Znf_GATA IPR013088, Znf_NHR/GATA |
PANTHERi | PTHR10071, PTHR10071, 1 hit PTHR10071:SF190, PTHR10071:SF190, 1 hit |
Pfami | View protein in Pfam PF00320, GATA, 2 hits |
PRINTSi | PR00619, GATAZNFINGER |
SMARTi | View protein in SMART SM00401, ZnF_GATA, 2 hits |
PROSITEi | View protein in PROSITE PS00344, GATA_ZN_FINGER_1, 2 hits PS50114, GATA_ZN_FINGER_2, 2 hits |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MDFPGLGALG TSEPLPQFVD SALVSSTSDS AGFFSSGPES LDTASSSTSP
60 70 80 90 100
NAATAAATAL AYYREAEAYR HSPVFQVYPL LNSMEGIPGS SPYASWAYSK
110 120 130 140 150
TALYPASTVC PSHEDAPSQT LEDPDGKNNN TFLETLKTER LSPDLLTLGT
160 170 180 190 200
ALPTSLPVTS SAYGGADFPS PFFSPTGSPL SSAAYSSPKF HGSLPLAPCE
210 220 230 240 250
ARECVNCGAT ATPLWRRDRT GHYLCNACGL YHKMNGQNRP LIRPKKRMIV
260 270 280 290 300
SKRAGTQCTN CQTTTTTLWR RNASGDPVCN ACGLYYKLHQ VNRPLTMRKD
310 320 330 340 350
GIQTRNRKAS GKGKKKRGSS LAGAGAAEGP AGGFMVVAGG SSSGNCGEVA
360 370 380 390 400
PGLTLGTAGT AHLYQGLGPV VLSGPVSHLM SFPGPLLGSP TASFPTGPVP
410
TTTSTSVVSP LSS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D13518 mRNA Translation: BAA02735.1 |
PIRi | S48756 |
RefSeqi | NP_036896.1, NM_012764.1 XP_017457744.1, XM_017602255.1 |
Genome annotation databases
Ensembli | ENSRNOT00000029272; ENSRNOP00000038111; ENSRNOG00000025534 ENSRNOT00000075082; ENSRNOP00000067336; ENSRNOG00000047663 |
GeneIDi | 25172 |
KEGGi | rno:25172 |
UCSCi | RGD:2663, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D13518 mRNA Translation: BAA02735.1 |
PIRi | S48756 |
RefSeqi | NP_036896.1, NM_012764.1 XP_017457744.1, XM_017602255.1 |
3D structure databases
SMRi | P43429 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000038111 |
PTM databases
PhosphoSitePlusi | P43429 |
Proteomic databases
PaxDbi | P43429 |
Genome annotation databases
Ensembli | ENSRNOT00000029272; ENSRNOP00000038111; ENSRNOG00000025534 ENSRNOT00000075082; ENSRNOP00000067336; ENSRNOG00000047663 |
GeneIDi | 25172 |
KEGGi | rno:25172 |
UCSCi | RGD:2663, rat |
Organism-specific databases
CTDi | 2623 |
RGDi | 2663, Gata1 |
Phylogenomic databases
eggNOGi | KOG1601, Eukaryota |
GeneTreei | ENSGT00940000161156 |
HOGENOMi | CLU_027524_1_1_1 |
InParanoidi | P43429 |
OMAi | YSKTGLY |
OrthoDBi | 807790at2759 |
PhylomeDBi | P43429 |
TreeFami | TF315391 |
Enzyme and pathway databases
Reactomei | R-RNO-8936459, RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function R-RNO-8939236, RUNX1 regulates transcription of genes involved in differentiation of HSCs R-RNO-983231, Factors involved in megakaryocyte development and platelet production |
Miscellaneous databases
PROi | PR:P43429 |
Gene expression databases
Bgeei | ENSRNOG00000025534, Expressed in spleen and 14 other tissues |
Genevisiblei | P43429, RN |
Family and domain databases
CDDi | cd00202, ZnF_GATA, 2 hits |
Gene3Di | 3.30.50.10, 2 hits |
InterProi | View protein in InterPro IPR029524, GATA-1 IPR039355, Transcription_factor_GATA IPR000679, Znf_GATA IPR013088, Znf_NHR/GATA |
PANTHERi | PTHR10071, PTHR10071, 1 hit PTHR10071:SF190, PTHR10071:SF190, 1 hit |
Pfami | View protein in Pfam PF00320, GATA, 2 hits |
PRINTSi | PR00619, GATAZNFINGER |
SMARTi | View protein in SMART SM00401, ZnF_GATA, 2 hits |
PROSITEi | View protein in PROSITE PS00344, GATA_ZN_FINGER_1, 2 hits PS50114, GATA_ZN_FINGER_2, 2 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | GATA1_RAT | |
Accessioni | P43429Primary (citable) accession number: P43429 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | November 1, 1995 | |
Last modified: | February 23, 2022 | |
This is version 163 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |