UniProtKB - P43422 (MTV1_GEOSE)
Protein
Modification methylase BstVI
Gene
bstVIM
Organism
Geobacillus stearothermophilus (Bacillus stearothermophilus)
Status
Functioni
This methylase recognizes the double-stranded sequence CTCGAG, causes specific methylation on A-5 on both strands, and protects the DNA from cleavage by the BstVI endonuclease.
Catalytic activityi
- a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an N6-methyl-2'-deoxyadenosine in DNA + H+ + S-adenosyl-L-homocysteineEC:2.1.1.72
GO - Molecular functioni
- DNA binding Source: InterPro
- site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC
GO - Biological processi
- DNA restriction-modification system Source: UniProtKB-KW
Keywordsi
Molecular function | Methyltransferase, Transferase |
Biological process | Restriction system |
Ligand | S-adenosyl-L-methionine |
Protein family/group databases
REBASEi | 3331, M.BstVI |
Names & Taxonomyi
Protein namesi | Recommended name: Modification methylase BstVI (EC:2.1.1.72)Short name: M.BstVI Alternative name(s): Adenine-specific methyltransferase BstVI |
Gene namesi | Name:bstVIM |
Organismi | Geobacillus stearothermophilus (Bacillus stearothermophilus) |
Taxonomic identifieri | 1422 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Geobacillus |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000087945 | 1 – 561 | Modification methylase BstVIAdd BLAST | 561 |
Proteomic databases
PRIDEi | P43422 |
Family & Domainsi
Sequence similaritiesi
Belongs to the N(4)/N(6)-methyltransferase family.Curated
Family and domain databases
InterProi | View protein in InterPro IPR002052, DNA_methylase_N6_adenine_CS IPR011639, RM_methylase_Eco57I IPR029063, SAM-dependent_MTases IPR025931, TaqI_C |
Pfami | View protein in Pfam PF07669, Eco57I, 1 hit PF12950, TaqI_C, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
PROSITEi | View protein in PROSITE PS00092, N6_MTASE, 1 hit |
i Sequence
Sequence statusi: Complete.
P43422-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVTQHSYRMH GAVYTKPIIV DLILDLTGYT SDKNLENFKL LDPSFGDGVF
60 70 80 90 100
LEAAVHRLMD SLIRRGYRPN ELIDHLGNCI RGIELRLEAY QAGRHRLQKV
110 120 130 140 150
LEGYGFSKPE INWLINQWII QADFLLWQED TTEAIKFDFV VGNPPYVRQE
160 170 180 190 200
LIQDELIKKY RKRYTTIYDR ADLYVPFIQH SLELLSEQGT LGIICSDRFT
210 220 230 240 250
KNRYGKKLRK FITDNYKVRY IVDLHKTSPF ENEVTAYPAI YVIKTKNYDK
260 270 280 290 300
SVVRAVYTEV ITSKVCQDAK DFLLSNQKPD QSSKEMKTYV FSEWFAGDEP
310 320 330 340 350
WIIQSQECRE ILRRLENRFP LIEDDVHSCK IRIGVATGAD KVYIVDPQQV
360 370 380 390 400
DIEPEVLLPL VTTADISSGR IIWSGKHVIN PFNSDGGLIN LDDFPRLKTY
410 420 430 440 450
FQQHEEIIKN RNVAKKNPSQ WFRTIDRIYP EIVHQPKLLI PDMKNTNHIV
460 470 480 490 500
KDDGAFYPHH NLYYILPGNW NIDILRAILL SSVVKFFIWS YATKMRGDTL
510 520 530 540 550
RYQAQYLRKI RLPDPKSLTN DQKERLMDER VIQSQEYLDS IVAEIYQLSK
560
TEIEIIKDAL E
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L07642 Genomic DNA Translation: AAA51408.1 |
PIRi | JN0797 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L07642 Genomic DNA Translation: AAA51408.1 |
PIRi | JN0797 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein family/group databases
REBASEi | 3331, M.BstVI |
Proteomic databases
PRIDEi | P43422 |
Miscellaneous databases
PROi | PR:P43422 |
Family and domain databases
InterProi | View protein in InterPro IPR002052, DNA_methylase_N6_adenine_CS IPR011639, RM_methylase_Eco57I IPR029063, SAM-dependent_MTases IPR025931, TaqI_C |
Pfami | View protein in Pfam PF07669, Eco57I, 1 hit PF12950, TaqI_C, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
PROSITEi | View protein in PROSITE PS00092, N6_MTASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MTV1_GEOSE | |
Accessioni | P43422Primary (citable) accession number: P43422 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | November 1, 1995 | |
Last modified: | December 2, 2020 | |
This is version 69 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Documents
- SIMILARITY comments
Index of protein domains and families - Restriction enzymes and methylases
Classification of restriction enzymes and methylases and list of entries