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UniProtKB - P43367 (CAN2_PIG)
Protein
Calpain-2 catalytic subunit
Gene
CAPN2
Organism
Sus scrofa (Pig)
Status
Functioni
Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves MYOC at 'Arg-226'. Proteolytically cleaves CPEB3 following neuronal stimulation which abolishes CPEB3 translational repressor activity, leading to translation of CPEB3 target mRNAs.
By similarityCatalytic activityi
- Broad endopeptidase specificity. EC:3.4.22.53
Cofactori
Ca2+By similarityNote: Binds 7 Ca2+ ions.By similarity
Activity regulationi
Activated by 200-1000 micromolar concentrations of calcium and inhibited by calpastatin.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 166 | Calcium 5; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 169 | Calcium 5By similarity | 1 | |
Metal bindingi | 171 | Calcium 5; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 176 | Calcium 5By similarity | 1 | |
Metal bindingi | 209 | Calcium 6By similarity | 1 | |
Metal bindingi | 211 | Calcium 6By similarity | 1 | |
Metal bindingi | 213 | Calcium 6; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 215 | Calcium 6; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 220 | Calcium 6By similarity | 1 | |
Metal bindingi | 239 | Calcium 7PROSITE-ProRule annotation | 1 | |
Metal bindingi | 241 | Calcium 7PROSITE-ProRule annotation | 1 | |
Metal bindingi | 243 | Calcium 7; via carbonyl oxygenPROSITE-ProRule annotation | 1 | |
Metal bindingi | 245 | Calcium 7; via carbonyl oxygenPROSITE-ProRule annotation | 1 | |
Metal bindingi | 250 | Calcium 7PROSITE-ProRule annotation | 1 | |
Metal bindingi | 282 | Calcium 1By similarity | 1 | |
Metal bindingi | 285 | Calcium 1By similarity | 1 |
GO - Molecular functioni
- calcium-dependent cysteine-type endopeptidase activity Source: UniProtKB
- calcium ion binding Source: InterPro
GO - Biological processi
- cellular response to amino acid stimulus Source: UniProtKB
- proteolysis Source: UniProtKB
- regulation of cytoskeleton organization Source: InterPro
Keywordsi
Molecular function | Hydrolase, Protease, Thiol protease |
Ligand | Calcium, Metal-binding |
Enzyme and pathway databases
BRENDAi | 3.4.22.53, 6170 |
Protein family/group databases
MEROPSi | C95.001 |
Names & Taxonomyi
Protein namesi | Recommended name: Calpain-2 catalytic subunit (EC:3.4.22.53)Alternative name(s): Calcium-activated neutral proteinase 2 Short name: CANP 2 Calpain M-type Calpain-2 large subunit Millimolar-calpain Short name: M-calpain |
Gene namesi | Name:CAPN2 |
Organismi | Sus scrofa (Pig) |
Taxonomic identifieri | 9823 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Suina › Suidae › Sus |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm By similarity
Plasma membrane
- Cell membrane By similarity
Note: Translocates to the plasma membrane upon Ca2+ binding.By similarity
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
Keywords - Cellular componenti
Cell membrane, Cytoplasm, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000207703 | ‹1 – 324 | Calpain-2 catalytic subunitAdd BLAST | ›324 |
Proteomic databases
PeptideAtlasi | P43367 |
PRIDEi | P43367 |
Expressioni
Tissue specificityi
Ubiquitous.
Interactioni
Subunit structurei
Forms a heterodimer with a small (regulatory) subunit (CAPNS1).
Interacts with CPEB3; this leads to cleavage of CPEB3.
By similarityProtein-protein interaction databases
STRINGi | 9823.ENSSSCP00000026311 |
Chemistry databases
BindingDBi | P43367 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 190 – 224 | EF-hand 1CuratedAdd BLAST | 35 | |
Domaini | 226 – 261 | EF-hand 2PROSITE-ProRule annotationAdd BLAST | 36 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | ‹1 – 138 | Domain IIIAdd BLAST | ›138 | |
Regioni | 139 – 153 | LinkerAdd BLAST | 15 | |
Regioni | 158 – 324 | Domain IVAdd BLAST | 167 |
Sequence similaritiesi
Belongs to the peptidase C2 family.Curated
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG0045, Eukaryota |
InParanoidi | P43367 |
Family and domain databases
CDDi | cd00214, Calpain_III, 1 hit |
InterProi | View protein in InterPro IPR033883, C2_III IPR022682, Calpain_domain_III IPR022683, Calpain_III IPR036213, Calpain_III_sf IPR029539, CAPN2 IPR011992, EF-hand-dom_pair IPR018247, EF_Hand_1_Ca_BS IPR002048, EF_hand_dom |
PANTHERi | PTHR10183:SF268, PTHR10183:SF268, 1 hit |
Pfami | View protein in Pfam PF01067, Calpain_III, 1 hit PF13833, EF-hand_8, 1 hit |
SMARTi | View protein in SMART SM00720, calpain_III, 1 hit |
SUPFAMi | SSF47473, SSF47473, 1 hit SSF49758, SSF49758, 1 hit |
PROSITEi | View protein in PROSITE PS00018, EF_HAND_1, 1 hit PS50222, EF_HAND_2, 1 hit |
i Sequence
Sequence statusi: Fragment.
P43367-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
YPNTFWMNPQ YLIKLEEEDE DQEDGESGCT FLVGLIQKHR RRQRKMGEDM
60 70 80 90 100
HTIGFGIYEV PEELTGQTNI HLSKNFFLTH RARERSDTFI NLREVLNRFK
110 120 130 140 150
LPPGEYILVP STFEPNKDGD FCIRVFSEKK ADYQVVDDEI EADLEENDAS
160 170 180 190 200
EDDIDDGFRR LFAQLAGEDA EISAFELQTI LRRVLAKRQD IKSDGFSIET
210 220 230 240 250
CRIMVDMLDS DGSAKLGLKE FYILWTKIQK YQKIYREIDV DRSGTMNSYE
260 270 280 290 300
MRKALEEAGF KLPCQLHQVI VARFADDQLI IDFDNFVRCL VRLETLFRIS
310 320
KQLDSENTGT IELDLISWLC FSVL
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Non-terminal residuei | 1 | 1 | ||
Sequence conflicti | 202 | R → K in AAB17381 (PubMed:9728040).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U01181 mRNA Translation: AAC48401.1 U71320 mRNA Translation: AAB17381.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U01181 mRNA Translation: AAC48401.1 U71320 mRNA Translation: AAB17381.1 |
3D structure databases
AlphaFoldDBi | P43367 |
SMRi | P43367 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9823.ENSSSCP00000026311 |
Chemistry databases
BindingDBi | P43367 |
ChEMBLi | CHEMBL4143 |
Protein family/group databases
MEROPSi | C95.001 |
Proteomic databases
PeptideAtlasi | P43367 |
PRIDEi | P43367 |
Phylogenomic databases
eggNOGi | KOG0045, Eukaryota |
InParanoidi | P43367 |
Enzyme and pathway databases
BRENDAi | 3.4.22.53, 6170 |
Family and domain databases
CDDi | cd00214, Calpain_III, 1 hit |
InterProi | View protein in InterPro IPR033883, C2_III IPR022682, Calpain_domain_III IPR022683, Calpain_III IPR036213, Calpain_III_sf IPR029539, CAPN2 IPR011992, EF-hand-dom_pair IPR018247, EF_Hand_1_Ca_BS IPR002048, EF_hand_dom |
PANTHERi | PTHR10183:SF268, PTHR10183:SF268, 1 hit |
Pfami | View protein in Pfam PF01067, Calpain_III, 1 hit PF13833, EF-hand_8, 1 hit |
SMARTi | View protein in SMART SM00720, calpain_III, 1 hit |
SUPFAMi | SSF47473, SSF47473, 1 hit SSF49758, SSF49758, 1 hit |
PROSITEi | View protein in PROSITE PS00018, EF_HAND_1, 1 hit PS50222, EF_HAND_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CAN2_PIG | |
Accessioni | P43367Primary (citable) accession number: P43367 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | November 1, 1997 | |
Last modified: | May 25, 2022 | |
This is version 144 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - SIMILARITY comments
Index of protein domains and families