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UniProtKB - P43276 (H15_MOUSE)
Protein
Histone H1.5
Gene
H1-5
Organism
Mus musculus (Mouse)
Status
Functioni
Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity).
By similarityGO - Molecular functioni
- chromatin DNA binding Source: MGI
- DNA binding Source: MGI
- double-stranded DNA binding Source: GO_Central
- histone deacetylase binding Source: MGI
- nucleosomal DNA binding Source: GO_Central
GO - Biological processi
- chromatin organization Source: MGI
- chromosome condensation Source: GO_Central
- establishment of protein localization to chromatin Source: MGI
- muscle organ development Source: MGI
- negative regulation of DNA recombination Source: GO_Central
- negative regulation of transcription by RNA polymerase II Source: MGI
- nucleosome assembly Source: InterPro
- nucleosome positioning Source: GO_Central
- positive regulation of cell growth Source: MGI
- positive regulation of histone H3-K9 methylation Source: MGI
- protein stabilization Source: MGI
Keywordsi
Molecular function | DNA-binding |
Enzyme and pathway databases
Reactomei | R-MMU-140342, Apoptosis induced DNA fragmentation R-MMU-2559584, Formation of Senescence-Associated Heterochromatin Foci (SAHF) |
Names & Taxonomyi
Protein namesi | Recommended name: Histone H1.5Alternative name(s): H1 VAR.5 H1b |
Gene namesi | Name:H1-5 Synonyms:H1f5, Hist1h1b |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1861461, H1f5 |
VEuPathDBi | HostDB:ENSMUSG00000058773 |
Subcellular locationi
Nucleus
Other locations
Note: Mainly localizes in heterochromatin.By similarity
Nucleus
- nucleus Source: MGI
Other locations
- chromatin Source: MGI
- heterochromatin Source: MGI
- nucleosome Source: InterPro
Keywords - Cellular componenti
Chromosome, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000195918 | 2 – 223 | Histone H1.5Add BLAST | 222 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineCombined sources | 1 | |
Modified residuei | 2 | PhosphoserineBy similarity | 1 | |
Modified residuei | 17 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 18 | PhosphoserineCombined sources | 1 | |
Modified residuei | 27 | N6-methyllysineBy similarity | 1 | |
Modified residuei | 34 | N6-(beta-hydroxybutyryl)lysine; alternateBy similarity | 1 | |
Modified residuei | 34 | N6-succinyllysine; alternateCombined sources | 1 | |
Modified residuei | 36 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 46 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 52 | N6-(beta-hydroxybutyryl)lysineBy similarity | 1 | |
Modified residuei | 54 | Citrulline1 Publication | 1 | |
Modified residuei | 64 | N6-(beta-hydroxybutyryl)lysineBy similarity | 1 | |
Modified residuei | 75 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 85 | N6-(beta-hydroxybutyryl)lysineBy similarity | 1 | |
Modified residuei | 90 | N6-(beta-hydroxybutyryl)lysineBy similarity | 1 | |
Modified residuei | 106 | N6-(beta-hydroxybutyryl)lysineBy similarity | 1 | |
Modified residuei | 135 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 152 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 165 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 170 | PhosphoserineBy similarity | 1 | |
Modified residuei | 186 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
H1 histones are progressively phosphorylated during the cell cycle, becoming maximally phosphorylated during late G2 phase and M phase, and being dephosphorylated sharply thereafter.By similarity
Citrullination at Arg-54 (H1R54ci) by PADI4 takes place within the DNA-binding site of H1 and results in its displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance.1 Publication
Hydroxybutyrylation of histones is induced by starvation.By similarity
Keywords - PTMi
Acetylation, Citrullination, Hydroxylation, Methylation, PhosphoproteinProteomic databases
EPDi | P43276 |
jPOSTi | P43276 |
PaxDbi | P43276 |
PeptideAtlasi | P43276 |
PRIDEi | P43276 |
ProteomicsDBi | 269705 |
PTM databases
iPTMneti | P43276 |
PhosphoSitePlusi | P43276 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000058773, Expressed in heart and 82 other tissues |
Interactioni
Binary interactionsi
P43276
With | #Exp. | IntAct |
---|---|---|
Msx1 [P13297] | 3 | EBI-903960,EBI-903969 |
GO - Molecular functioni
- histone deacetylase binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 208131, 11 interactors |
IntActi | P43276, 4 interactors |
STRINGi | 10090.ENSMUSP00000079356 |
Miscellaneous databases
RNActi | P43276, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 36 – 109 | H15PROSITE-ProRule annotationAdd BLAST | 74 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 56 | DisorderedSequence analysisAdd BLAST | 56 | |
Regioni | 91 – 223 | DisorderedSequence analysisAdd BLAST | 133 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 133 – 223 | Basic residuesSequence analysisAdd BLAST | 91 |
Domaini
The C-terminal domain is required for high-affinity binding to chromatin.By similarity
Sequence similaritiesi
Belongs to the histone H1/H5 family.PROSITE-ProRule annotation
Phylogenomic databases
eggNOGi | KOG4012, Eukaryota |
GeneTreei | ENSGT00940000162950 |
HOGENOMi | CLU_052897_7_0_1 |
InParanoidi | P43276 |
OMAi | ANENVQP |
OrthoDBi | 1565299at2759 |
PhylomeDBi | P43276 |
TreeFami | TF313664 |
Family and domain databases
CDDi | cd00073, H15, 1 hit |
Gene3Di | 1.10.10.10, 1 hit |
InterProi | View protein in InterPro IPR005819, H1/H5 IPR005818, Histone_H1/H5_H15 IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
Pfami | View protein in Pfam PF00538, Linker_histone, 1 hit |
PRINTSi | PR00624, HISTONEH5 |
SMARTi | View protein in SMART SM00526, H15, 1 hit |
SUPFAMi | SSF46785, SSF46785, 1 hit |
PROSITEi | View protein in PROSITE PS51504, H15, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P43276-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSETAPAETA APAPVEKSPA KKKTTKKAGA AKRKATGPPV SELITKAVSA
60 70 80 90 100
SKERGGVSLP ALKKALAAGG YDVEKNNSRI KLGLKSLVSK GTLVQTKGTG
110 120 130 140 150
ASGSFKLNKK AASGEAKPKA KKTGAAKAKK PAGATPKKPK KTAGAKKTVK
160 170 180 190 200
KTPKKAKKPA AAGVKKVAKS PKKAKAAAKP KKAAKSPAKP KAVKSKASKP
210 220
KVTKPKTAKP KAAKAKKAVS KKK
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 61 | A → T in BAB32001 (PubMed:12408966).Curated | 1 | |
Sequence conflicti | 120 | A → V in BAB32001 (PubMed:12408966).Curated | 1 | |
Sequence conflicti | 137 | K → N in BAB32001 (PubMed:12408966).Curated | 1 | |
Sequence conflicti | 195 | S → F in BAB32001 (PubMed:12408966).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z46227 Genomic DNA Translation: CAA86299.1 U62922 Genomic DNA Translation: AAB05798.1 AY158904 Genomic DNA Translation: AAO06215.1 AK020117 mRNA Translation: BAB32001.1 |
CCDSi | CCDS26295.1 |
PIRi | A35245 S49492 |
RefSeqi | NP_064418.1, NM_020034.2 |
Genome annotation databases
Ensembli | ENSMUST00000080511; ENSMUSP00000079356; ENSMUSG00000058773 |
GeneIDi | 56702 |
KEGGi | mmu:56702 |
UCSCi | uc007pri.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z46227 Genomic DNA Translation: CAA86299.1 U62922 Genomic DNA Translation: AAB05798.1 AY158904 Genomic DNA Translation: AAO06215.1 AK020117 mRNA Translation: BAB32001.1 |
CCDSi | CCDS26295.1 |
PIRi | A35245 S49492 |
RefSeqi | NP_064418.1, NM_020034.2 |
3D structure databases
SMRi | P43276 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 208131, 11 interactors |
IntActi | P43276, 4 interactors |
STRINGi | 10090.ENSMUSP00000079356 |
PTM databases
iPTMneti | P43276 |
PhosphoSitePlusi | P43276 |
Proteomic databases
EPDi | P43276 |
jPOSTi | P43276 |
PaxDbi | P43276 |
PeptideAtlasi | P43276 |
PRIDEi | P43276 |
ProteomicsDBi | 269705 |
Protocols and materials databases
Antibodypediai | 54587, 222 antibodies from 22 providers |
DNASUi | 56702 |
Genome annotation databases
Ensembli | ENSMUST00000080511; ENSMUSP00000079356; ENSMUSG00000058773 |
GeneIDi | 56702 |
KEGGi | mmu:56702 |
UCSCi | uc007pri.2, mouse |
Organism-specific databases
CTDi | 56702 |
MGIi | MGI:1861461, H1f5 |
VEuPathDBi | HostDB:ENSMUSG00000058773 |
Phylogenomic databases
eggNOGi | KOG4012, Eukaryota |
GeneTreei | ENSGT00940000162950 |
HOGENOMi | CLU_052897_7_0_1 |
InParanoidi | P43276 |
OMAi | ANENVQP |
OrthoDBi | 1565299at2759 |
PhylomeDBi | P43276 |
TreeFami | TF313664 |
Enzyme and pathway databases
Reactomei | R-MMU-140342, Apoptosis induced DNA fragmentation R-MMU-2559584, Formation of Senescence-Associated Heterochromatin Foci (SAHF) |
Miscellaneous databases
BioGRID-ORCSi | 56702, 3 hits in 64 CRISPR screens |
PROi | PR:P43276 |
RNActi | P43276, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000058773, Expressed in heart and 82 other tissues |
Family and domain databases
CDDi | cd00073, H15, 1 hit |
Gene3Di | 1.10.10.10, 1 hit |
InterProi | View protein in InterPro IPR005819, H1/H5 IPR005818, Histone_H1/H5_H15 IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
Pfami | View protein in Pfam PF00538, Linker_histone, 1 hit |
PRINTSi | PR00624, HISTONEH5 |
SMARTi | View protein in SMART SM00526, H15, 1 hit |
SUPFAMi | SSF46785, SSF46785, 1 hit |
PROSITEi | View protein in PROSITE PS51504, H15, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | H15_MOUSE | |
Accessioni | P43276Primary (citable) accession number: P43276 Secondary accession number(s): Q9CRM8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 23, 2022 | |
This is version 171 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families