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UniProtKB - P43274 (H14_MOUSE)
Protein
Histone H1.4
Gene
H1-4
Organism
Mus musculus (Mouse)
Status
Functioni
Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation.
2 PublicationsGO - Molecular functioni
- ADP binding Source: MGI
- AMP binding Source: MGI
- ATP binding Source: MGI
- calcium ion binding Source: MGI
- chromatin DNA binding Source: MGI
- dATP binding Source: MGI
- DNA binding Source: MGI
- double-stranded DNA binding Source: MGI
- GTP binding Source: MGI
- nucleosomal DNA binding Source: GO_Central
- nucleotide binding Source: MGI
GO - Biological processi
- chromosome condensation Source: GO_Central
- histone H3-K27 trimethylation Source: MGI
- histone H3-K4 trimethylation Source: MGI
- negative regulation of DNA recombination Source: GO_Central
- negative regulation of transcription by RNA polymerase II Source: MGI
- nucleosome assembly Source: InterPro
- nucleosome positioning Source: MGI
- regulation of transcription by RNA polymerase II Source: MGI
Keywordsi
Molecular function | DNA-binding |
Protein family/group databases
MoonProti | P43274 |
Names & Taxonomyi
Protein namesi | Recommended name: Histone H1.4Alternative name(s): H1 VAR.2 H1e |
Gene namesi | Name:H1-4By similarity Synonyms:H1f4Imported, Hist1h1eImported |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1931527, H1f4 |
VEuPathDBi | HostDB:ENSMUSG00000051627 |
Subcellular locationi
Nucleus
Other locations
Note: Mainly localizes in heterochromatin. Dysplays a punctuate staining pattern in the nucleus.By similarity
Nucleus
- nucleus Source: UniProtKB
Other locations
- heterochromatin Source: MGI
- nucleosome Source: MGI
Keywords - Cellular componenti
Chromosome, NucleusPathology & Biotechi
Disruption phenotypei
Triple-deficient mice (H1-2, H1-3 and H1-4) die by midgestation with a broad range of defects. These embryos have about 50% of the normal ratio of H1 to nucleosomes. This proves at least that a correct stoichiometry of linker histone deposition on chromatin is essential.2 Publications
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000195917 | 2 – 219 | Histone H1.4Add BLAST | 218 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineCombined sources | 1 | |
Modified residuei | 2 | PhosphoserineBy similarity | 1 | |
Modified residuei | 17 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 18 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 26 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 26 | N6-methyllysine; alternateBy similarity | 1 | |
Modified residuei | 34 | N6-(beta-hydroxybutyryl)lysine; alternateBy similarity | 1 | |
Modified residuei | 34 | N6-succinyllysine; alternateCombined sources | 1 | |
Modified residuei | 36 | PhosphoserineCombined sources | 1 | |
Modified residuei | 52 | N6-(beta-hydroxybutyryl)lysineBy similarity | 1 | |
Modified residuei | 54 | Citrulline1 Publication | 1 | |
Modified residuei | 64 | N6-(beta-hydroxybutyryl)lysineBy similarity | 1 | |
Modified residuei | 85 | N6-(beta-hydroxybutyryl)lysineBy similarity | 1 | |
Modified residuei | 90 | N6-(beta-hydroxybutyryl)lysineBy similarity | 1 | |
Modified residuei | 106 | N6-(beta-hydroxybutyryl)lysineBy similarity | 1 | |
Modified residuei | 146 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 150 | ADP-ribosylserineBy similarity | 1 | |
Modified residuei | 187 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Citrullination at Arg-54 (H1R54ci) by PADI4 takes place within the DNA-binding site of H1 and results in its displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance.1 Publication
ADP-ribosylated on Ser-55, Ser-113 and Ser-150 in response to DNA damage.By similarity
H1 histones are progressively phosphorylated during the cell cycle, becoming maximally phosphorylated during late G2 phase and M phase, and being dephosphorylated sharply thereafter.By similarity
Acetylated at Lys-26. Deacetylated at Lys-26 by SIRT1.By similarity
Hydroxybutyrylation of histones is induced by starvation.By similarity
Keywords - PTMi
Acetylation, ADP-ribosylation, Citrullination, Hydroxylation, Methylation, PhosphoproteinProteomic databases
EPDi | P43274 |
jPOSTi | P43274 |
MaxQBi | P43274 |
PaxDbi | P43274 |
PeptideAtlasi | P43274 |
PRIDEi | P43274 |
ProteomicsDBi | 269642 |
PTM databases
iPTMneti | P43274 |
PhosphoSitePlusi | P43274 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000051627, Expressed in hindbrain and 129 other tissues |
Genevisiblei | P43274, MM |
Interactioni
Protein-protein interaction databases
BioGRIDi | 206062, 12 interactors |
IntActi | P43274, 9 interactors |
MINTi | P43274 |
STRINGi | 10090.ENSMUSP00000057308 |
Miscellaneous databases
RNActi | P43274, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 36 – 109 | H15PROSITE-ProRule annotationAdd BLAST | 74 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 41 | DisorderedSequence analysisAdd BLAST | 41 | |
Regioni | 92 – 219 | DisorderedSequence analysisAdd BLAST | 128 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 150 – 219 | Basic residuesSequence analysisAdd BLAST | 70 |
Domaini
The C-terminal domain is required for high-affinity binding to chromatin.By similarity
Sequence similaritiesi
Belongs to the histone H1/H5 family.PROSITE-ProRule annotation
Phylogenomic databases
eggNOGi | KOG4012, Eukaryota |
GeneTreei | ENSGT00940000155501 |
HOGENOMi | CLU_052897_7_0_1 |
InParanoidi | P43274 |
OMAi | PTWADMI |
OrthoDBi | 1565299at2759 |
PhylomeDBi | P43274 |
TreeFami | TF313664 |
Family and domain databases
CDDi | cd00073, H15, 1 hit |
Gene3Di | 1.10.10.10, 1 hit |
InterProi | View protein in InterPro IPR005819, H1/H5 IPR005818, Histone_H1/H5_H15 IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
Pfami | View protein in Pfam PF00538, Linker_histone, 1 hit |
PRINTSi | PR00624, HISTONEH5 |
SMARTi | View protein in SMART SM00526, H15, 1 hit |
SUPFAMi | SSF46785, SSF46785, 1 hit |
PROSITEi | View protein in PROSITE PS51504, H15, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P43274-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSETAPAAPA APAPAEKTPV KKKARKAAGG AKRKTSGPPV SELITKAVAA
60 70 80 90 100
SKERSGVSLA ALKKALAAAG YDVEKNNSRI KLGLKSLVSK GTLVQTKGTG
110 120 130 140 150
ASGSFKLNKK AASGEAKPKA KRAGAAKAKK PAGAAKKPKK AAGTATAKKS
160 170 180 190 200
TKKTPKKAKK PAAAAGAKKA KSPKKAKATK AKKAPKSPAK AKTVKPKAAK
210
PKTSKPKAAK PKKTAAKKK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L26163 Genomic DNA Translation: AAA37760.1 L04141 Genomic DNA Translation: AAA37814.1 Y12292 Genomic DNA Translation: CAA72971.1 BC089600 mRNA Translation: AAH89600.1 |
CCDSi | CCDS26355.1 |
PIRi | I49742 |
RefSeqi | NP_056602.1, NM_015787.4 |
Genome annotation databases
Ensembli | ENSMUST00000062045; ENSMUSP00000057308; ENSMUSG00000051627 |
GeneIDi | 50709 |
KEGGi | mmu:50709 |
UCSCi | uc007puj.3, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L26163 Genomic DNA Translation: AAA37760.1 L04141 Genomic DNA Translation: AAA37814.1 Y12292 Genomic DNA Translation: CAA72971.1 BC089600 mRNA Translation: AAH89600.1 |
CCDSi | CCDS26355.1 |
PIRi | I49742 |
RefSeqi | NP_056602.1, NM_015787.4 |
3D structure databases
SMRi | P43274 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 206062, 12 interactors |
IntActi | P43274, 9 interactors |
MINTi | P43274 |
STRINGi | 10090.ENSMUSP00000057308 |
Protein family/group databases
MoonProti | P43274 |
PTM databases
iPTMneti | P43274 |
PhosphoSitePlusi | P43274 |
Proteomic databases
EPDi | P43274 |
jPOSTi | P43274 |
MaxQBi | P43274 |
PaxDbi | P43274 |
PeptideAtlasi | P43274 |
PRIDEi | P43274 |
ProteomicsDBi | 269642 |
Protocols and materials databases
Antibodypediai | 25545, 478 antibodies from 22 providers |
DNASUi | 50709 |
Genome annotation databases
Ensembli | ENSMUST00000062045; ENSMUSP00000057308; ENSMUSG00000051627 |
GeneIDi | 50709 |
KEGGi | mmu:50709 |
UCSCi | uc007puj.3, mouse |
Organism-specific databases
CTDi | 50709 |
MGIi | MGI:1931527, H1f4 |
VEuPathDBi | HostDB:ENSMUSG00000051627 |
Phylogenomic databases
eggNOGi | KOG4012, Eukaryota |
GeneTreei | ENSGT00940000155501 |
HOGENOMi | CLU_052897_7_0_1 |
InParanoidi | P43274 |
OMAi | PTWADMI |
OrthoDBi | 1565299at2759 |
PhylomeDBi | P43274 |
TreeFami | TF313664 |
Miscellaneous databases
BioGRID-ORCSi | 50709, 6 hits in 64 CRISPR screens |
PROi | PR:P43274 |
RNActi | P43274, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000051627, Expressed in hindbrain and 129 other tissues |
Genevisiblei | P43274, MM |
Family and domain databases
CDDi | cd00073, H15, 1 hit |
Gene3Di | 1.10.10.10, 1 hit |
InterProi | View protein in InterPro IPR005819, H1/H5 IPR005818, Histone_H1/H5_H15 IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
Pfami | View protein in Pfam PF00538, Linker_histone, 1 hit |
PRINTSi | PR00624, HISTONEH5 |
SMARTi | View protein in SMART SM00526, H15, 1 hit |
SUPFAMi | SSF46785, SSF46785, 1 hit |
PROSITEi | View protein in PROSITE PS51504, H15, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | H14_MOUSE | |
Accessioni | P43274Primary (citable) accession number: P43274 Secondary accession number(s): Q5EBH3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 23, 2022 | |
This is version 176 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families