UniProtKB - P43267 (SOX15_MOUSE)
Protein
Protein SOX-15
Gene
Sox15
Organism
Mus musculus (Mouse)
Status
Functioni
Transcription factor that binds to DNA at the 5'-AACAATG-3' consensus sequence (PubMed:10821863, PubMed:15863505, PubMed:16759287, PubMed:17363903). Acts as a transcriptional activator and repressor (PubMed:10821863, PubMed:15863505, PubMed:16759287). Binds synergistically with POU5F1 (OCT3/4) to gene promoters (PubMed:15863505). Binds to the FOXK1 promoter and recruits FHL3, resulting in transcriptional activation of FOXK1 which leads to myoblast proliferation (PubMed:17363903). Acts as an inhibitor of myoblast differentiation via transcriptional repression which leads to down-regulation of the muscle-specific genes MYOD and MYOG (PubMed:10821863). Involved in trophoblast giant cell differentiation via enhancement of HAND1 transcriptional activity (PubMed:16759287). Regulates transcription of HRC via binding to it proximal enhancer region (PubMed:15863505). Involved in skeletal muscle regeneration (PubMed:15367664, PubMed:17363903). Also plays a role in the development of myogenic precursor cells (PubMed:15367664).5 Publications
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 47 – 115 | HMG boxPROSITE-ProRule annotationAdd BLAST | 69 |
GO - Molecular functioni
- chromatin binding Source: MGI
- DNA binding Source: MGI
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: MGI
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: MGI
GO - Biological processi
- anatomical structure morphogenesis Source: GO_Central
- cell differentiation Source: MGI
- myoblast development Source: MGI
- negative regulation of striated muscle tissue development Source: MGI
- negative regulation of transcription by RNA polymerase II Source: MGI
- positive regulation of G0 to G1 transition Source: BHF-UCL
- positive regulation of myoblast proliferation Source: BHF-UCL
- positive regulation of satellite cell activation involved in skeletal muscle regeneration Source: BHF-UCL
- positive regulation of transcription by RNA polymerase II Source: MGI
- regulation of transcription, DNA-templated Source: GO_Central
- skeletal muscle tissue regeneration Source: MGI
- trophoblast giant cell differentiation Source: UniProtKB
Keywordsi
Molecular function | Activator, DNA-binding, Repressor |
Biological process | Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: Protein SOX-15 |
Gene namesi | Name:Sox15 Synonyms:Sox-15 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:98363, Sox15 |
Subcellular locationi
Nucleus
- Nucleus PROSITE-ProRule annotation3 Publications
Nucleus
- nuclear chromatin Source: GO_Central
- nucleus Source: MGI
Other locations
- cytoplasm Source: MGI
- transcription regulator complex Source: MGI
Keywords - Cellular componenti
NucleusPathology & Biotechi
Disruption phenotypei
Knockout mice are viable, morphologically normal and fertile (PubMed:15367664, PubMed:15863505). Reduced regeneration of damaged skeletal muscle fibers following injury with increased numbers of mononuclear cells and a significant reduction in the number of myofibers (PubMed:15367664, PubMed:17363903). Impaired proliferation of myogenic progenitor cells, reduced number of satellite cells within the tibialis anterior muscles, and a decrease in FOXK1 expression (PubMed:17363903).3 Publications
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000048763 | 1 – 231 | Protein SOX-15Add BLAST | 231 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 35 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | P43267 |
PRIDEi | P43267 |
PTM databases
PhosphoSitePlusi | P43267 |
Expressioni
Tissue specificityi
Expressed in myoblasts (at protein level) (PubMed:15367664). Expressed in embryonic stem cells (at protein level) (PubMed:15367664, PubMed:15863505). Expressed in myogenic progenitor cells (at protein level) (PubMed:17363903). Expressed in the ovary (PubMed:15367664). Expressed in kidney, liver, skeletal muscle, and testes (PubMed:10821863, PubMed:15367664). Expressed in lung and skin (PubMed:15863505). Expressed in the brain, heart, diaphragm, and intestines (PubMed:10821863). Expressed in the conceptus tissues of the placenta (PubMed:16759287).5 Publications
Developmental stagei
Highly expressed in the conceptus ectoplacental cone of the placenta at embryonic day 7.5 (E7.5) (PubMed:16759287). Expressed in the conceptus trophoblast giant cell layer of the placenta (PubMed:16759287). Expressed in the trophoblast giant cells of the placenta from E10, expression peaks at E14, then reduces thereafter to E18 (PubMed:16759287). Expression is increased during trophoblast differentiation (PubMed:16759287). Expressed at 8.5 dpc in developing embryos, with increased expression at 9.5 dpc (PubMed:10821863).2 Publications
Gene expression databases
Bgeei | ENSMUSG00000041287, Expressed in testis and 153 other tissues |
ExpressionAtlasi | P43267, baseline and differential |
Genevisiblei | P43267, MM |
Interactioni
Subunit structurei
Interacts with HAND1; the interaction enhances HAND1-induced differentiation of trophoblast giant cells (PubMed:16759287).
Interacts with POU5F1 (OCT3/4); binds synergistically with POU5F1 to DNA (PubMed:15863505).
Interacts with FHL3; the interaction recruits the transcriptional coactivator FHL3 to the FOXK1 promoter (PubMed:17363903).
3 PublicationsBinary interactionsi
P43267
With | #Exp. | IntAct |
---|---|---|
Fhl3 [Q9R059] | 7 | EBI-7332587,EBI-7332617 |
Protein-protein interaction databases
BioGRIDi | 203402, 5 interactors |
IntActi | P43267, 1 interactor |
MINTi | P43267 |
STRINGi | 10090.ENSMUSP00000048524 |
Miscellaneous databases
RNActi | P43267, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 45 | Required to promote HAND1 transcriptional activator activity1 PublicationAdd BLAST | 45 | |
Regioni | 136 – 181 | Interaction with FHL31 PublicationAdd BLAST | 46 |
Phylogenomic databases
eggNOGi | KOG0527, Eukaryota |
GeneTreei | ENSGT00940000162099 |
HOGENOMi | CLU_106341_0_0_1 |
InParanoidi | P43267 |
OMAi | HCKPEAP |
OrthoDBi | 369754at2759 |
PhylomeDBi | P43267 |
TreeFami | TF351735 |
Family and domain databases
Gene3Di | 1.10.30.10, 1 hit |
InterProi | View protein in InterPro IPR009071, HMG_box_dom IPR036910, HMG_box_dom_sf IPR031269, SOX15 |
PANTHERi | PTHR10270:SF45, PTHR10270:SF45, 1 hit |
Pfami | View protein in Pfam PF00505, HMG_box, 1 hit |
SMARTi | View protein in SMART SM00398, HMG, 1 hit |
SUPFAMi | SSF47095, SSF47095, 1 hit |
PROSITEi | View protein in PROSITE PS50118, HMG_BOX_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P43267-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MALTSSSQAE TWSLHPRAST ASLPLGPQEQ EAGGSPGASG GLPLEKVKRP
60 70 80 90 100
MNAFMVWSSV QRRQMAQQNP KMHNSEISKR LGAQWKLLGD EEKRPFVEEA
110 120 130 140 150
KRLRARHLRD YPDYKYRPRR KSKNSSTGSV PFSQEGGGLA CGGSHWGPGY
160 170 180 190 200
TTTQGSRGFG YQPPNYSTAY LPGSYTSSHC RPEAPLPCTF PQSDPRLQGE
210 220 230
LRPSFSPYLS PDSSTPYNTS LAGAPMPVTH L
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 71 | K → E in BAA28604 (PubMed:10524236).Curated | 1 | |
Sequence conflicti | 97 | V → M in CAB37848 (PubMed:8479922).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 135 | E → G in strain: various strains. | 1 | |
Natural varianti | 224 | A → S in strain: various strains. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB014474 Genomic DNA Translation: BAA28604.1 AF182945 mRNA Translation: AAF72108.1 AB039214 Genomic DNA Translation: BAB68738.1 AB039215 Genomic DNA Translation: BAB68739.1 AB039216 Genomic DNA Translation: BAB68740.1 AB039217 Genomic DNA Translation: BAB68741.1 AB039218 Genomic DNA Translation: BAB68742.1 AB039219 Genomic DNA Translation: BAB68743.1 AB039220 Genomic DNA Translation: BAB68744.1 AB039221 Genomic DNA Translation: BAB68745.1 AB039222 Genomic DNA Translation: BAB68746.1 U70443 mRNA Translation: AAC52861.1 X70909 Genomic DNA Translation: CAB37848.1 X98369 mRNA Translation: CAA67015.1 |
CCDSi | CCDS24902.1 |
PIRi | S33715 |
RefSeqi | NP_033261.1, NM_009235.2 |
Genome annotation databases
Ensembli | ENSMUST00000047373; ENSMUSP00000048524; ENSMUSG00000041287 |
GeneIDi | 20670 |
KEGGi | mmu:20670 |
UCSCi | uc007jqu.2, mouse |
Keywords - Coding sequence diversityi
PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB014474 Genomic DNA Translation: BAA28604.1 AF182945 mRNA Translation: AAF72108.1 AB039214 Genomic DNA Translation: BAB68738.1 AB039215 Genomic DNA Translation: BAB68739.1 AB039216 Genomic DNA Translation: BAB68740.1 AB039217 Genomic DNA Translation: BAB68741.1 AB039218 Genomic DNA Translation: BAB68742.1 AB039219 Genomic DNA Translation: BAB68743.1 AB039220 Genomic DNA Translation: BAB68744.1 AB039221 Genomic DNA Translation: BAB68745.1 AB039222 Genomic DNA Translation: BAB68746.1 U70443 mRNA Translation: AAC52861.1 X70909 Genomic DNA Translation: CAB37848.1 X98369 mRNA Translation: CAA67015.1 |
CCDSi | CCDS24902.1 |
PIRi | S33715 |
RefSeqi | NP_033261.1, NM_009235.2 |
3D structure databases
SMRi | P43267 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 203402, 5 interactors |
IntActi | P43267, 1 interactor |
MINTi | P43267 |
STRINGi | 10090.ENSMUSP00000048524 |
PTM databases
PhosphoSitePlusi | P43267 |
Proteomic databases
PaxDbi | P43267 |
PRIDEi | P43267 |
Protocols and materials databases
Antibodypediai | 12118, 194 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000047373; ENSMUSP00000048524; ENSMUSG00000041287 |
GeneIDi | 20670 |
KEGGi | mmu:20670 |
UCSCi | uc007jqu.2, mouse |
Organism-specific databases
CTDi | 6665 |
MGIi | MGI:98363, Sox15 |
Phylogenomic databases
eggNOGi | KOG0527, Eukaryota |
GeneTreei | ENSGT00940000162099 |
HOGENOMi | CLU_106341_0_0_1 |
InParanoidi | P43267 |
OMAi | HCKPEAP |
OrthoDBi | 369754at2759 |
PhylomeDBi | P43267 |
TreeFami | TF351735 |
Miscellaneous databases
BioGRID-ORCSi | 20670, 1 hit in 17 CRISPR screens |
PROi | PR:P43267 |
RNActi | P43267, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000041287, Expressed in testis and 153 other tissues |
ExpressionAtlasi | P43267, baseline and differential |
Genevisiblei | P43267, MM |
Family and domain databases
Gene3Di | 1.10.30.10, 1 hit |
InterProi | View protein in InterPro IPR009071, HMG_box_dom IPR036910, HMG_box_dom_sf IPR031269, SOX15 |
PANTHERi | PTHR10270:SF45, PTHR10270:SF45, 1 hit |
Pfami | View protein in Pfam PF00505, HMG_box, 1 hit |
SMARTi | View protein in SMART SM00398, HMG, 1 hit |
SUPFAMi | SSF47095, SSF47095, 1 hit |
PROSITEi | View protein in PROSITE PS50118, HMG_BOX_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SOX15_MOUSE | |
Accessioni | P43267Primary (citable) accession number: P43267 Secondary accession number(s): O70204 Q9JLG2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | December 8, 2000 | |
Last modified: | December 2, 2020 | |
This is version 154 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot