UniProtKB - P43261 (EAE_ECO57)

BLAST

>sp|P43261|EAE_ECO57 Intimin OS=Escherichia coli O157:H7 OX=83334 GN=eae PE=1 SV=2
MITHGCYTRTRHKHKLKKTLIMLSAGLGLFFYVNQNSFANGENYFKLGSDSKLLTHDSYQ
NRLFYTLKTGETVADLSKSQDINLSTIWSLNKHLYSSESEMMKAAPGQQIILPLKKLPFE
YSALPLLGSAPLVAAGGVAGHTNKLTKMSPDVTKSNMTDDKALNYAAQQAASLGSQLQSR
SLNGDYAKDTALGIAGNQASSQLQAWLQHYGTAEVNLQSGNNFDGSSLDFLLPFYDSEKM
LAFGQVGARYIDSRFTANLGAGQRFFLPANMLGYNVFIDQDFSGDNTRLGIGGEYWRDYF
KSSVNGYFRMSGWHESYNKKDYDERPANGFDIRFNGYLPSYPALGAKLIYEQYYGDNVAL
FNSDKLQSNPGAATVGVNYTPIPLVTMGIDYRHGTGNENDLLYSMQFRYQFDKSWSQQIE
PQYVNELRTLSGSRYDLVQRNNNIILEYKKQDILSLNIPHDINGTEHSTQKIQLIVKSKY
GLDRIVWDDSALRSQGGQIQHSGSQSAQDYQAILPAYVQGGSNIYKVTARAYDRNGNSSN
NVQLTITVLSNGQVVDQVGVTDFTADKTSAKADNADTITYTATVKKNGVAQANVPVSFNI
VSGTATLGANSAKTDANGKATVTLKSSTPGQVVVSAKTAEMTSALNASAVIFFDQTKASI
TEIKADKTTAVANGKDAIKYTVKVMKNGQPVNNQSVTFSTNFGMFNGKSQTQATTGNDGR
ATITLTSSSAGKATVSATVSDGAEVKATEVTFFDELKIDNKVDIIGNNVRGELPNIWLQY
GQFKLKASGGDGTYSWYSENTSIATVDASGKVTLNGKGSVVIKATSGDKQTVSYTIKAPS
YMIKVDKQAYYADAMSICKNLLPSTQTVLSDIYDSWGAANKYSHYSSMNSITAWIKQTSS
EQRSGVSSTYNLITQNPLPGVNVNTPNVYAVCVE

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History
Entry version 139 (12 Sep 2018)
Sequence version 2 (29 Aug 2001)
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Protein

Intimin

Gene

eae

Organism

Escherichia coli O157:H7

Status

Reviewed-Annotation score: -Experimental evidence at protein levelⁱ

Functionⁱ

Necessary for the production of attaching and effacing lesions on tissue culture cells.

GO - Biological processⁱ

cell adhesion Source: InterPro
pathogenesis
Source: CACAO
Inferred from mutant phenotypeⁱ
- 8376595

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Biological process	Virulence

Biological process

Virulence

Protein family/group databases

TCDBⁱ	1.B.54.1.1 the intimin/invasin (int/inv) or autotransporter-3 (at-3) family

TCDBⁱ

1.B.54.1.1 the intimin/invasin (int/inv) or autotransporter-3 (at-3) family

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Intimin Alternative name(s): Attaching and effacing protein Short name: Eae protein Gamma-intimin
Gene namesⁱ	Name:eae Synonyms:eaeA Ordered Locus Names:Z5110, ECs4559 ORF Names:L0025
Organismⁱ	Escherichia coli O157:H7
Taxonomic identifierⁱ	83334 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › Escherichia coli
Proteomesⁱ	UP000000558 Componentⁱ: Chromosome UP000002519 Componentⁱ: Chromosome

Subcellular locationⁱ

Cell outer membrane By similarity; Single-pass membrane protein By similarity

Topology

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Transmembraneⁱ	20 – 39	HelicalSequence analysisAdd BLAST		20

GO - Cellular componentⁱ

cell outer membrane Source: UniProtKB-SubCell
integral component of membrane Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

Keywords - Cellular componentⁱ

Cell outer membrane, Membrane

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000211828	1 – 934	IntiminAdd BLAST		934

Interactionⁱ

Protein-protein interaction databases

STRINGⁱ	155864.Z5110

STRINGⁱ

155864.Z5110

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Beta strandⁱ	212 – 221	Combined sources	10
Beta strandⁱ	225 – 236	Combined sources	12
Beta strandⁱ	238 – 251	Combined sources	14
Beta strandⁱ	254 – 266	Combined sources	13
Beta strandⁱ	271 – 281	Combined sources	11
Turnⁱ	282 – 285	Combined sources	4
Beta strandⁱ	286 – 297	Combined sources	12
Beta strandⁱ	300 – 309	Combined sources	10
Beta strandⁱ	317 – 319	Combined sources	3
Beta strandⁱ	323 – 326	Combined sources	4
Beta strandⁱ	329 – 338	Combined sources	10
Beta strandⁱ	341 – 353	Combined sources	13
Beta strandⁱ	355 – 358	Combined sources	4
Beta strandⁱ	363 – 365	Combined sources	3
Beta strandⁱ	367 – 369	Combined sources	3
Beta strandⁱ	371 – 382	Combined sources	12
Beta strandⁱ	385 – 394	Combined sources	10
Helixⁱ	395 – 397	Combined sources	3
Beta strandⁱ	398 – 410	Combined sources	13
Helixⁱ	415 – 418	Combined sources	4
Helixⁱ	423 – 428	Combined sources	6
Turnⁱ	430 – 432	Combined sources	3
Beta strandⁱ	446 – 448	Combined sources	3
Beta strandⁱ	760 – 764	Combined sources	5
Turnⁱ	765 – 768	Combined sources	4
Beta strandⁱ	769 – 772	Combined sources	4
Beta strandⁱ	775 – 778	Combined sources	4
Beta strandⁱ	782 – 784	Combined sources	3
Beta strandⁱ	787 – 789	Combined sources	3
Beta strandⁱ	794 – 799	Combined sources	6
Turnⁱ	801 – 803	Combined sources	3
Beta strandⁱ	804 – 806	Combined sources	3
Beta strandⁱ	810 – 814	Combined sources	5
Beta strandⁱ	816 – 818	Combined sources	3
Beta strandⁱ	820 – 826	Combined sources	7
Turnⁱ	827 – 829	Combined sources	3
Beta strandⁱ	831 – 836	Combined sources	6
Beta strandⁱ	842 – 848	Combined sources	7
Helixⁱ	851 – 857	Combined sources	7
Turnⁱ	858 – 860	Combined sources	3
Helixⁱ	866 – 876	Combined sources	11
Helixⁱ	879 – 881	Combined sources	3
Helixⁱ	883 – 885	Combined sources	3
Beta strandⁱ	892 – 894	Combined sources	3
Helixⁱ	899 – 904	Combined sources	6
Beta strandⁱ	906 – 911	Combined sources	6
Turnⁱ	912 – 914	Combined sources	3
Beta strandⁱ	917 – 922	Combined sources	6
Beta strandⁱ	929 – 933	Combined sources	5

Show more details

3D structure databases

ProteinModelPortalⁱ	P43261
SMRⁱ	P43261
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ	P43261

EvolutionaryTraceⁱ

P43261

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Length
Domainⁱ	63 – 112	LysMPROSITE-ProRule annotationAdd BLAST	50
Domainⁱ	560 – 653	Big-1 1PROSITE-ProRule annotationAdd BLAST	94
Domainⁱ	660 – 753	Big-1 2PROSITE-ProRule annotationAdd BLAST	94
Domainⁱ	787 – 834	BIG2Sequence analysisAdd BLAST	48

Sequence similaritiesⁱ

Belongs to the intimin/invasin family.Curated

Keywords - Domainⁱ

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGⁱ	ENOG4105TNG Bacteria ENOG410XQE6 LUCA
HOGENOMⁱ	HOG000217915
KEGG Orthology (KO) More...KOⁱ	K12790
OMAⁱ	PENMLGY

Family and domain databases

Gene3Dⁱ	2.40.160.160, 1 hit 2.60.40.10, 2 hits 3.10.100.10, 1 hit
InterProⁱ	View protein in InterPro IPR003344 Big_1_dom IPR003343 Big_2 IPR016186 C-type_lectin-like/link_sf IPR016187 CTDL_fold IPR024519 IAT_beta IPR038177 IAT_beta_sf IPR013783 Ig-like_fold IPR003535 Intimin/invasin_bac IPR013117 Intimin_C IPR008964 Invasin/intimin_cell_adhesion IPR018392 LysM_dom
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF02369 Big_1, 2 hits PF02368 Big_2, 1 hit PF11924 IAT_beta, 1 hit PF07979 Intimin_C, 1 hit PF01476 LysM, 1 hit
PRINTSⁱ	PR01369 INTIMIN
SMARTⁱ	View protein in SMART SM00634 BID_1, 2 hits SM00635 BID_2, 1 hit SM00257 LysM, 1 hit
SUPFAMⁱ	SSF49373 SSF49373, 3 hits SSF56436 SSF56436, 1 hit
PROSITEⁱ	View protein in PROSITE PS51127 BIG1, 2 hits PS51782 LYSM, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

P43261-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MITHGCYTRT RHKHKLKKTL IMLSAGLGLF FYVNQNSFAN GENYFKLGSD 
        60         70         80         90        100
SKLLTHDSYQ NRLFYTLKTG ETVADLSKSQ DINLSTIWSL NKHLYSSESE 
       110        120        130        140        150
MMKAAPGQQI ILPLKKLPFE YSALPLLGSA PLVAAGGVAG HTNKLTKMSP 
       160        170        180        190        200
DVTKSNMTDD KALNYAAQQA ASLGSQLQSR SLNGDYAKDT ALGIAGNQAS 
       210        220        230        240        250
SQLQAWLQHY GTAEVNLQSG NNFDGSSLDF LLPFYDSEKM LAFGQVGARY 
       260        270        280        290        300
IDSRFTANLG AGQRFFLPAN MLGYNVFIDQ DFSGDNTRLG IGGEYWRDYF 
       310        320        330        340        350
KSSVNGYFRM SGWHESYNKK DYDERPANGF DIRFNGYLPS YPALGAKLIY 
       360        370        380        390        400
EQYYGDNVAL FNSDKLQSNP GAATVGVNYT PIPLVTMGID YRHGTGNEND 
       410        420        430        440        450
LLYSMQFRYQ FDKSWSQQIE PQYVNELRTL SGSRYDLVQR NNNIILEYKK 
       460        470        480        490        500
QDILSLNIPH DINGTEHSTQ KIQLIVKSKY GLDRIVWDDS ALRSQGGQIQ 
       510        520        530        540        550
HSGSQSAQDY QAILPAYVQG GSNIYKVTAR AYDRNGNSSN NVQLTITVLS 
       560        570        580        590        600
NGQVVDQVGV TDFTADKTSA KADNADTITY TATVKKNGVA QANVPVSFNI 
       610        620        630        640        650
VSGTATLGAN SAKTDANGKA TVTLKSSTPG QVVVSAKTAE MTSALNASAV 
       660        670        680        690        700
IFFDQTKASI TEIKADKTTA VANGKDAIKY TVKVMKNGQP VNNQSVTFST 
       710        720        730        740        750
NFGMFNGKSQ TQATTGNDGR ATITLTSSSA GKATVSATVS DGAEVKATEV 
       760        770        780        790        800
TFFDELKIDN KVDIIGNNVR GELPNIWLQY GQFKLKASGG DGTYSWYSEN 
       810        820        830        840        850
TSIATVDASG KVTLNGKGSV VIKATSGDKQ TVSYTIKAPS YMIKVDKQAY 
       860        870        880        890        900
YADAMSICKN LLPSTQTVLS DIYDSWGAAN KYSHYSSMNS ITAWIKQTSS 
       910        920        930 
EQRSGVSSTY NLITQNPLPG VNVNTPNVYA VCVE

Length:934

Mass (Da):101,836

Last modified:August 29, 2001 - v2

Checksum:ⁱBE73686D7F79D3C9

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	221	N → D in CAA77642 (PubMed:1552854).Curated	1
Sequence conflictⁱ	311 – 312	SG → RR in CAA77642 (PubMed:1552854).Curated	2
Sequence conflictⁱ	318	N → H in CAA77642 (PubMed:1552854).Curated	1
Sequence conflictⁱ	642	T → S in CAA77642 (PubMed:1552854).Curated	1
Sequence conflictⁱ	769	V → VK (PubMed:1577255).Curated	1
Sequence conflictⁱ	771 – 772	GE → SM (PubMed:1577255).Curated	2
Sequence conflictⁱ	903	R → S in AAA21468 (PubMed:8005211).Curated	1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	Z11541 Genomic DNA Translation: CAA77642.1 X60439 Genomic DNA Translation: CAA42967.1 AF071034 Genomic DNA Translation: AAC31504.1 AF081182 Genomic DNA Translation: AAD05498.1 AF081183 Genomic DNA Translation: AAD05499.1 AE005174 Genomic DNA Translation: AAG58823.1 BA000007 Genomic DNA Translation: BAB37982.1 U32312 Genomic DNA Translation: AAB00111.1 L08095 Genomic DNA Translation: AAA21468.1
PIRⁱ	C86045 G91198 I41193
NCBI Reference Sequences More...RefSeqⁱ	NP_312586.1, NC_002695.1 WP_000627885.1, NZ_NOKN01000002.1

Genome annotation databases

EnsemblBacteriaⁱ	AAG58823; AAG58823; Z5110 BAB37982; BAB37982; BAB37982
GeneIDⁱ	915471
KEGGⁱ	ece:Z5110 ecs:ECs4559
PATRICⁱ	fig\|386585.9.peg.4776

Protein	Similar proteins		Species	Score	Length	Source
P43261	Gamma-intimin		ECO57		934	UniRef100_P43261
Gamma intimin		ECOLX		934
Intimin type gamma		ECO5C		934
Intimin type gamma		Escherichia coli O157:H7 str. SS52		934
Gamma intimin		Escherichia coli Xuzhou21		934
+19

Protein	Similar proteins		Species	Score	Length	Source
P43261	Gamma-intimin		ECO57		934	UniRef90_P43261
Gamma intimin		ECOLX		934
Intimin type gamma		ECO5C		934
Intimin type gamma		Escherichia coli O157:H7 str. SS52		934
Gamma intimin		Escherichia coli Xuzhou21		934
+75

Protein	Similar proteins		Species	Score	Length	Source
P43261	Intimin	)	ECO27		939	UniRef50_P43261
Intimin		CITFR		936
Intimin		ECO11		935
Gamma-intimin		ECO57		934
Gamma intimin		ECOLX		934
+463

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	Z11541 Genomic DNA Translation: CAA77642.1 X60439 Genomic DNA Translation: CAA42967.1 AF071034 Genomic DNA Translation: AAC31504.1 AF081182 Genomic DNA Translation: AAD05498.1 AF081183 Genomic DNA Translation: AAD05499.1 AE005174 Genomic DNA Translation: AAG58823.1 BA000007 Genomic DNA Translation: BAB37982.1 U32312 Genomic DNA Translation: AAB00111.1 L08095 Genomic DNA Translation: AAA21468.1
PIRⁱ	C86045 G91198 I41193
RefSeqⁱ	NP_312586.1, NC_002695.1 WP_000627885.1, NZ_NOKN01000002.1

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	2ZQK	X-ray	2.80	A/B	747-934	[»]
	2ZWK	X-ray	3.10	A/C/E	752-934	[»]
	3NCW	X-ray	2.80	A/B/C/D	747-934	[»]
	3NCX	X-ray	2.60	A/B	747-934	[»]
	4E1S	X-ray	1.86	A	208-449	[»]
	5G26	X-ray	2.42	A	208-449	[»]
ProteinModelPortalⁱ	P43261
SMRⁱ	P43261
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

STRINGⁱ	155864.Z5110

STRINGⁱ

155864.Z5110

Protein family/group databases

TCDBⁱ	1.B.54.1.1 the intimin/invasin (int/inv) or autotransporter-3 (at-3) family

TCDBⁱ

1.B.54.1.1 the intimin/invasin (int/inv) or autotransporter-3 (at-3) family

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	AAG58823; AAG58823; Z5110 BAB37982; BAB37982; BAB37982
GeneIDⁱ	915471
KEGGⁱ	ece:Z5110 ecs:ECs4559
PATRICⁱ	fig\|386585.9.peg.4776

Phylogenomic databases

eggNOGⁱ	ENOG4105TNG Bacteria ENOG410XQE6 LUCA
HOGENOMⁱ	HOG000217915
KOⁱ	K12790
OMAⁱ	PENMLGY

Miscellaneous databases

EvolutionaryTraceⁱ	P43261

EvolutionaryTraceⁱ

P43261

Family and domain databases

Gene3Dⁱ	2.40.160.160, 1 hit 2.60.40.10, 2 hits 3.10.100.10, 1 hit
InterProⁱ	View protein in InterPro IPR003344 Big_1_dom IPR003343 Big_2 IPR016186 C-type_lectin-like/link_sf IPR016187 CTDL_fold IPR024519 IAT_beta IPR038177 IAT_beta_sf IPR013783 Ig-like_fold IPR003535 Intimin/invasin_bac IPR013117 Intimin_C IPR008964 Invasin/intimin_cell_adhesion IPR018392 LysM_dom
Pfamⁱ	View protein in Pfam PF02369 Big_1, 2 hits PF02368 Big_2, 1 hit PF11924 IAT_beta, 1 hit PF07979 Intimin_C, 1 hit PF01476 LysM, 1 hit
PRINTSⁱ	PR01369 INTIMIN
SMARTⁱ	View protein in SMART SM00634 BID_1, 2 hits SM00635 BID_2, 1 hit SM00257 LysM, 1 hit
SUPFAMⁱ	SSF49373 SSF49373, 3 hits SSF56436 SSF56436, 1 hit
PROSITEⁱ	View protein in PROSITE PS51127 BIG1, 2 hits PS51782 LYSM, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	EAE_ECO57
Accessionⁱ	P43261Primary (citable) accession number: P43261 Secondary accession number(s): O85627, Q47168
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
	Last sequence update:	August 29, 2001
	Last modified:	September 12, 2018
	This is version 139 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

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UniParc

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UniRef

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Supporting data

UniProtKB - P43261 (EAE_ECO57)

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Intimin

eae

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Protein family/group databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Topology

GO - Cellular componenti

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

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Functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ