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UniProtKB - P43261 (EAE_ECO57)
Protein
Intimin
Gene
eae
Organism
Escherichia coli O157:H7
Status
Functioni
Necessary for the production of attaching and effacing lesions on tissue culture cells.
GO - Biological processi
- cell adhesion Source: InterPro
Keywordsi
Biological process | Virulence |
Protein family/group databases
TCDBi | 1.B.54.1.1, the intimin/invasin (int/inv) or autotransporter-3 (at-3) family |
Names & Taxonomyi
Protein namesi | Recommended name: IntiminAlternative name(s): Attaching and effacing protein Short name: Eae protein Gamma-intimin |
Gene namesi | Name:eae Synonyms:eaeA Ordered Locus Names:Z5110, ECs4559 ORF Names:L0025 |
Organismi | Escherichia coli O157:H7 |
Taxonomic identifieri | 83334 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Other locations
- Cell outer membrane By similarity; Single-pass membrane protein By similarity
Other locations
- cell outer membrane Source: UniProtKB-SubCell
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 20 – 39 | HelicalSequence analysisAdd BLAST | 20 |
Keywords - Cellular componenti
Cell outer membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000211828 | 1 – 934 | IntiminAdd BLAST | 934 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | P43261 |
BMRBi | P43261 |
SMRi | P43261 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P43261 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 63 – 112 | LysMPROSITE-ProRule annotationAdd BLAST | 50 | |
Domaini | 560 – 653 | Big-1 1PROSITE-ProRule annotationAdd BLAST | 94 | |
Domaini | 660 – 753 | Big-1 2PROSITE-ProRule annotationAdd BLAST | 94 | |
Domaini | 787 – 834 | BIG2Sequence analysisAdd BLAST | 48 |
Sequence similaritiesi
Belongs to the intimin/invasin family.Curated
Keywords - Domaini
Repeat, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG5492, Bacteria |
HOGENOMi | CLU_000210_1_1_6 |
OMAi | PENMLGY |
Family and domain databases
Gene3Di | 2.40.160.160, 1 hit 2.60.40.10, 2 hits 3.10.100.10, 1 hit |
InterProi | View protein in InterPro IPR003344, Big_1_dom IPR003343, Big_2 IPR016186, C-type_lectin-like/link_sf IPR016187, CTDL_fold IPR024519, IAT_beta IPR038177, IAT_beta_sf IPR013783, Ig-like_fold IPR003535, Intimin/invasin_bac IPR013117, Intimin_C IPR008964, Invasin/intimin_cell_adhesion IPR018392, LysM_dom |
Pfami | View protein in Pfam PF02369, Big_1, 2 hits PF02368, Big_2, 1 hit PF11924, IAT_beta, 1 hit PF07979, Intimin_C, 1 hit PF01476, LysM, 1 hit |
PRINTSi | PR01369, INTIMIN |
SMARTi | View protein in SMART SM00634, BID_1, 2 hits SM00635, BID_2, 1 hit SM00257, LysM, 1 hit |
SUPFAMi | SSF49373, SSF49373, 3 hits SSF56436, SSF56436, 1 hit |
PROSITEi | View protein in PROSITE PS51127, BIG1, 2 hits PS51782, LYSM, 1 hit |
i Sequence
Sequence statusi: Complete.
P43261-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MITHGCYTRT RHKHKLKKTL IMLSAGLGLF FYVNQNSFAN GENYFKLGSD
60 70 80 90 100
SKLLTHDSYQ NRLFYTLKTG ETVADLSKSQ DINLSTIWSL NKHLYSSESE
110 120 130 140 150
MMKAAPGQQI ILPLKKLPFE YSALPLLGSA PLVAAGGVAG HTNKLTKMSP
160 170 180 190 200
DVTKSNMTDD KALNYAAQQA ASLGSQLQSR SLNGDYAKDT ALGIAGNQAS
210 220 230 240 250
SQLQAWLQHY GTAEVNLQSG NNFDGSSLDF LLPFYDSEKM LAFGQVGARY
260 270 280 290 300
IDSRFTANLG AGQRFFLPAN MLGYNVFIDQ DFSGDNTRLG IGGEYWRDYF
310 320 330 340 350
KSSVNGYFRM SGWHESYNKK DYDERPANGF DIRFNGYLPS YPALGAKLIY
360 370 380 390 400
EQYYGDNVAL FNSDKLQSNP GAATVGVNYT PIPLVTMGID YRHGTGNEND
410 420 430 440 450
LLYSMQFRYQ FDKSWSQQIE PQYVNELRTL SGSRYDLVQR NNNIILEYKK
460 470 480 490 500
QDILSLNIPH DINGTEHSTQ KIQLIVKSKY GLDRIVWDDS ALRSQGGQIQ
510 520 530 540 550
HSGSQSAQDY QAILPAYVQG GSNIYKVTAR AYDRNGNSSN NVQLTITVLS
560 570 580 590 600
NGQVVDQVGV TDFTADKTSA KADNADTITY TATVKKNGVA QANVPVSFNI
610 620 630 640 650
VSGTATLGAN SAKTDANGKA TVTLKSSTPG QVVVSAKTAE MTSALNASAV
660 670 680 690 700
IFFDQTKASI TEIKADKTTA VANGKDAIKY TVKVMKNGQP VNNQSVTFST
710 720 730 740 750
NFGMFNGKSQ TQATTGNDGR ATITLTSSSA GKATVSATVS DGAEVKATEV
760 770 780 790 800
TFFDELKIDN KVDIIGNNVR GELPNIWLQY GQFKLKASGG DGTYSWYSEN
810 820 830 840 850
TSIATVDASG KVTLNGKGSV VIKATSGDKQ TVSYTIKAPS YMIKVDKQAY
860 870 880 890 900
YADAMSICKN LLPSTQTVLS DIYDSWGAAN KYSHYSSMNS ITAWIKQTSS
910 920 930
EQRSGVSSTY NLITQNPLPG VNVNTPNVYA VCVE
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 221 | N → D in CAA77642 (PubMed:1552854).Curated | 1 | |
Sequence conflicti | 311 – 312 | SG → RR in CAA77642 (PubMed:1552854).Curated | 2 | |
Sequence conflicti | 318 | N → H in CAA77642 (PubMed:1552854).Curated | 1 | |
Sequence conflicti | 642 | T → S in CAA77642 (PubMed:1552854).Curated | 1 | |
Sequence conflicti | 769 | V → VK (PubMed:1577255).Curated | 1 | |
Sequence conflicti | 771 – 772 | GE → SM (PubMed:1577255).Curated | 2 | |
Sequence conflicti | 903 | R → S in AAA21468 (PubMed:8005211).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z11541 Genomic DNA Translation: CAA77642.1 X60439 Genomic DNA Translation: CAA42967.1 AF071034 Genomic DNA Translation: AAC31504.1 AF081182 Genomic DNA Translation: AAD05498.1 AF081183 Genomic DNA Translation: AAD05499.1 AE005174 Genomic DNA Translation: AAG58823.1 BA000007 Genomic DNA Translation: BAB37982.1 U32312 Genomic DNA Translation: AAB00111.1 L08095 Genomic DNA Translation: AAA21468.1 |
PIRi | C86045 G91198 I41193 |
RefSeqi | NP_312586.1, NC_002695.1 WP_000627885.1, NZ_SWKA01000005.1 |
Genome annotation databases
EnsemblBacteriai | AAG58823; AAG58823; Z5110 BAB37982; BAB37982; ECs_4559 |
GeneIDi | 915471 |
KEGGi | ece:Z5110 ecs:ECs4559 |
PATRICi | fig|386585.9.peg.4776 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z11541 Genomic DNA Translation: CAA77642.1 X60439 Genomic DNA Translation: CAA42967.1 AF071034 Genomic DNA Translation: AAC31504.1 AF081182 Genomic DNA Translation: AAD05498.1 AF081183 Genomic DNA Translation: AAD05499.1 AE005174 Genomic DNA Translation: AAG58823.1 BA000007 Genomic DNA Translation: BAB37982.1 U32312 Genomic DNA Translation: AAB00111.1 L08095 Genomic DNA Translation: AAA21468.1 |
PIRi | C86045 G91198 I41193 |
RefSeqi | NP_312586.1, NC_002695.1 WP_000627885.1, NZ_SWKA01000005.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2ZQK | X-ray | 2.80 | A/B | 747-934 | [»] | |
2ZWK | X-ray | 3.10 | A/C/E | 752-934 | [»] | |
3NCW | X-ray | 2.80 | A/B/C/D | 747-934 | [»] | |
3NCX | X-ray | 2.60 | A/B | 747-934 | [»] | |
4E1S | X-ray | 1.86 | A | 208-449 | [»] | |
5G26 | X-ray | 2.42 | A | 208-449 | [»] | |
AlphaFoldDBi | P43261 | |||||
BMRBi | P43261 | |||||
SMRi | P43261 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 155864.EDL933_4947 |
Protein family/group databases
TCDBi | 1.B.54.1.1, the intimin/invasin (int/inv) or autotransporter-3 (at-3) family |
Genome annotation databases
EnsemblBacteriai | AAG58823; AAG58823; Z5110 BAB37982; BAB37982; ECs_4559 |
GeneIDi | 915471 |
KEGGi | ece:Z5110 ecs:ECs4559 |
PATRICi | fig|386585.9.peg.4776 |
Phylogenomic databases
eggNOGi | COG5492, Bacteria |
HOGENOMi | CLU_000210_1_1_6 |
OMAi | PENMLGY |
Miscellaneous databases
EvolutionaryTracei | P43261 |
PHI-basei | PHI:7913 |
Family and domain databases
Gene3Di | 2.40.160.160, 1 hit 2.60.40.10, 2 hits 3.10.100.10, 1 hit |
InterProi | View protein in InterPro IPR003344, Big_1_dom IPR003343, Big_2 IPR016186, C-type_lectin-like/link_sf IPR016187, CTDL_fold IPR024519, IAT_beta IPR038177, IAT_beta_sf IPR013783, Ig-like_fold IPR003535, Intimin/invasin_bac IPR013117, Intimin_C IPR008964, Invasin/intimin_cell_adhesion IPR018392, LysM_dom |
Pfami | View protein in Pfam PF02369, Big_1, 2 hits PF02368, Big_2, 1 hit PF11924, IAT_beta, 1 hit PF07979, Intimin_C, 1 hit PF01476, LysM, 1 hit |
PRINTSi | PR01369, INTIMIN |
SMARTi | View protein in SMART SM00634, BID_1, 2 hits SM00635, BID_2, 1 hit SM00257, LysM, 1 hit |
SUPFAMi | SSF49373, SSF49373, 3 hits SSF56436, SSF56436, 1 hit |
PROSITEi | View protein in PROSITE PS51127, BIG1, 2 hits PS51782, LYSM, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | EAE_ECO57 | |
Accessioni | P43261Primary (citable) accession number: P43261 Secondary accession number(s): O85627, Q47168 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | August 29, 2001 | |
Last modified: | May 25, 2022 | |
This is version 155 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families