UniProtKB - P43154 (COLA_VIBAL)
Protein
Microbial collagenase
Gene
N/A
Organism
Vibrio alginolyticus
Status
Functioni
Catalytic activityi
- Digestion of native collagen in the triple helical region at Xaa-|-Gly bonds. With synthetic peptides, a preference is shown for Gly at P3 and P1', Pro and Ala at P2 and P2', and hydroxyproline, Ala or Arg at P3'. EC:3.4.24.3
Cofactori
Zn2+By similarityNote: Binds 1 zinc ion.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 477 | Zinc; catalyticPROSITE-ProRule annotation | 1 | |
Active sitei | 478 | PROSITE-ProRule annotation | 1 | |
Metal bindingi | 481 | Zinc; catalyticPROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- metalloendopeptidase activity Source: InterPro
- serine-type endopeptidase activity Source: UniProtKB-EC
- zinc ion binding Source: InterPro
GO - Biological processi
- collagen catabolic process Source: UniProtKB-KW
Keywordsi
Molecular function | Hydrolase, Metalloprotease, Protease |
Biological process | Collagen degradation |
Ligand | Metal-binding, Zinc |
Protein family/group databases
MEROPSi | M09.001 |
Names & Taxonomyi
Protein namesi | |
Organismi | Vibrio alginolyticus |
Taxonomic identifieri | 663 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio › |
Subcellular locationi
Extracellular region or secreted
Extracellular region or secreted
- extracellular region Source: UniProtKB-SubCell
Keywords - Cellular componenti
SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 21 | Sequence analysisAdd BLAST | 21 | |
PropeptideiPRO_0000028676 | 22 – 75 | Add BLAST | 54 | |
ChainiPRO_0000028677 | 76 – 814 | Microbial collagenaseAdd BLAST | 739 |
Post-translational modificationi
Proteolytic cleavage might yield three different active forms.
Keywords - PTMi
ZymogenProteomic databases
PRIDEi | P43154 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 609 – 697 | PKDPROSITE-ProRule annotationAdd BLAST | 89 |
Sequence similaritiesi
Belongs to the peptidase M9A family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | COG3291, Bacteria |
Family and domain databases
Gene3Di | 2.60.40.10, 1 hit |
InterProi | View protein in InterPro IPR013783, Ig-like_fold IPR013661, Peptidase_M9_N_dom IPR002169, Peptidase_M9A/M9B IPR022409, PKD/Chitinase_dom IPR000601, PKD_dom IPR035986, PKD_dom_sf |
Pfami | View protein in Pfam PF01752, Peptidase_M9, 1 hit PF08453, Peptidase_M9_N, 1 hit |
PRINTSi | PR00931, MICOLLPTASE |
SMARTi | View protein in SMART SM00089, PKD, 1 hit |
SUPFAMi | SSF49299, SSF49299, 1 hit |
PROSITEi | View protein in PROSITE PS50093, PKD, 1 hit PS00142, ZINC_PROTEASE, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P43154-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MELKILSVAI ATTLTSTGVF ALSEPVSQVT EQHAHSAHTH GVEFNRVEYQ
60 70 80 90 100
PTATLPIQPS KATRVQSLES LDESSTACDL EALVTESSNQ LISEILSQGA
110 120 130 140 150
TCVNQLFSAE SRIQESVFSS DHMYNIAKHT TTLAKGYTGG GSDELETLFL
160 170 180 190 200
YLRAGYYAEF YNDNISFIEW VTPAVKESVD AFVNTASFYE NSDRHGKVLS
210 220 230 240 250
EVIITMDSAG LQHAYLPQVT QWLTRWNDQY AQHWYMRNAV NGVFTILFGG
260 270 280 290 300
QWNEQFVQII GNQTDLAKAL GDFALRASSI GAEDEFMAAN AGRELGRLTK
310 320 330 340 350
YTGNASSVVK SQLSRIFEQY EMYGRGDAVW LAAADTASYY ADCSEFGICN
360 370 380 390 400
FETELKGLVL SQTYTCSPTI RILSQNMTQE QHAAACSKMG YEEGYFHQSL
410 420 430 440 450
ETGEQPVKDD HNTQLQVNIF DSSTDYGKYA GPIFDISTDN GGMYLEGDPS
460 470 480 490 500
QPGNIPNFIA YEASYANADH FVWNLEHEYV HYLDGRFDLY GGFSHPTEKI
510 520 530 540 550
VWWSEGIAEY VAQENDNQAA LETILDGSTY TLSEIFETTY DGFDVDRIYR
560 570 580 590 600
WGYLAVRFMF ENHKDDVNQM LVETRQGNWI NYKATITQWA NLYQSEFEQW
610 620 630 640 650
QQTLVSNGAP NAVITANSKG KVGESITFSS ENSTDPNGKI VSVLWDFGDG
660 670 680 690 700
STSTQTKPTH QYGSEGEYSV SLSVTDSEGL TATATHTVVI SALGGNDTLP
710 720 730 740 750
QDCAVQSKVS GGRLTAGEPV CLANQQTIWL SVPAVNESSN LAITTGNGTG
760 770 780 790 800
NLKLEYSNSG WPDDTNLHGW SDNIGNGECI TLSNQSNYWG YVKVSGDFEN
810
AAIVVDFDAQ KCRQ
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X62635 Genomic DNA Translation: CAA44501.1 |
PIRi | S19658 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X62635 Genomic DNA Translation: CAA44501.1 |
PIRi | S19658 |
3D structure databases
SMRi | P43154 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 1219076.N646_0375 |
Protein family/group databases
MEROPSi | M09.001 |
Proteomic databases
PRIDEi | P43154 |
Phylogenomic databases
eggNOGi | COG3291, Bacteria |
Family and domain databases
Gene3Di | 2.60.40.10, 1 hit |
InterProi | View protein in InterPro IPR013783, Ig-like_fold IPR013661, Peptidase_M9_N_dom IPR002169, Peptidase_M9A/M9B IPR022409, PKD/Chitinase_dom IPR000601, PKD_dom IPR035986, PKD_dom_sf |
Pfami | View protein in Pfam PF01752, Peptidase_M9, 1 hit PF08453, Peptidase_M9_N, 1 hit |
PRINTSi | PR00931, MICOLLPTASE |
SMARTi | View protein in SMART SM00089, PKD, 1 hit |
SUPFAMi | SSF49299, SSF49299, 1 hit |
PROSITEi | View protein in PROSITE PS50093, PKD, 1 hit PS00142, ZINC_PROTEASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | COLA_VIBAL | |
Accessioni | P43154Primary (citable) accession number: P43154 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | November 1, 1995 | |
Last modified: | December 2, 2020 | |
This is version 111 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencingDocuments
- Peptidase families
Classification of peptidase families and list of entries - SIMILARITY comments
Index of protein domains and families