UniProtKB - P43121 (MUC18_HUMAN)
Protein
Cell surface glycoprotein MUC18
Gene
MCAM
Organism
Homo sapiens (Human)
Status
Functioni
Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration.2 Publications
GO - Biological processi
- anatomical structure morphogenesis Source: ProtInc
- angiogenesis Source: MGI
- cell adhesion Source: ProtInc
- glomerular filtration Source: UniProtKB
- positive regulation of cell migration Source: Ensembl
- vascular wound healing Source: UniProtKB
Keywordsi
Biological process | Cell adhesion |
Enzyme and pathway databases
PathwayCommonsi | P43121 |
Names & Taxonomyi
Protein namesi | Recommended name: Cell surface glycoprotein MUC18Alternative name(s): Cell surface glycoprotein P1H12 Melanoma cell adhesion molecule Melanoma-associated antigen A32 Melanoma-associated antigen MUC18 S-endo 1 endothelial-associated antigen CD_antigen: CD146 |
Gene namesi | Name:MCAM Synonyms:MUC18 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000076706.14 |
HGNCi | HGNC:6934, MCAM |
MIMi | 155735, gene |
neXtProti | NX_P43121 |
Subcellular locationi
Other locations
Extracellular region or secreted
- extracellular region Source: BHF-UCL
- extracellular space Source: Ensembl
Nucleus
- nucleus Source: Ensembl
Plasma Membrane
- external side of plasma membrane Source: UniProtKB
- plasma membrane Source: ProtInc
Other locations
- focal adhesion Source: UniProtKB
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 24 – 559 | ExtracellularSequence analysisAdd BLAST | 536 | |
Transmembranei | 560 – 583 | HelicalSequence analysisAdd BLAST | 24 | |
Topological domaini | 584 – 646 | CytoplasmicSequence analysisAdd BLAST | 63 |
Keywords - Cellular componenti
MembranePathology & Biotechi
Organism-specific databases
DisGeNETi | 4162 |
OpenTargetsi | ENSG00000076706 |
PharmGKBi | PA30678 |
Miscellaneous databases
Pharosi | P43121, Tbio |
Chemistry databases
ChEMBLi | CHEMBL3712863 |
GuidetoPHARMACOLOGYi | 2988 |
Polymorphism and mutation databases
BioMutai | MCAM |
DMDMi | 85681878 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 23 | 1 PublicationAdd BLAST | 23 | |
ChainiPRO_0000014891 | 24 – 646 | Cell surface glycoprotein MUC18Add BLAST | 623 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 48 ↔ 116 | Curated | ||
Glycosylationi | 56 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 161 ↔ 223 | Curated | ||
Disulfide bondi | 272 ↔ 320 | Curated | ||
Disulfide bondi | 365 ↔ 407 | Curated | ||
Glycosylationi | 418 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 449 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 452 ↔ 499 | Curated | ||
Glycosylationi | 467 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Glycosylationi | 508 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 518 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 527 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 544 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Modified residuei | 606 | PhosphoserineBy similarity | 1 | |
Modified residuei | 614 | PhosphoserineCombined sources | 1 | |
Modified residuei | 628 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Disulfide bond, Glycoprotein, PhosphoproteinProteomic databases
EPDi | P43121 |
jPOSTi | P43121 |
MassIVEi | P43121 |
MaxQBi | P43121 |
PaxDbi | P43121 |
PeptideAtlasi | P43121 |
PRIDEi | P43121 |
ProteomicsDBi | 55589 [P43121-1] 55590 [P43121-2] |
PTM databases
GlyConnecti | 1104, 2 N-Linked glycans (2 sites) |
GlyGeni | P43121, 9 sites |
iPTMneti | P43121 |
PhosphoSitePlusi | P43121 |
SwissPalmi | P43121 |
Expressioni
Tissue specificityi
Detected in endothelial cells in vascular tissue throughout the body. May appear at the surface of neural crest cells during their embryonic migration. Appears to be limited to vascular smooth muscle in normal adult tissues. Associated with tumor progression and the development of metastasis in human malignant melanoma. Expressed most strongly on metastatic lesions and advanced primary tumors and is only rarely detected in benign melanocytic nevi and thin primary melanomas with a low probability of metastasis.
Gene expression databases
Bgeei | ENSG00000076706, Expressed in popliteal artery and 242 other tissues |
ExpressionAtlasi | P43121, baseline and differential |
Genevisiblei | P43121, HS |
Organism-specific databases
HPAi | ENSG00000076706, Low tissue specificity |
Interactioni
Protein-protein interaction databases
BioGRIDi | 110332, 29 interactors |
DIPi | DIP-52791N |
IntActi | P43121, 17 interactors |
MINTi | P43121 |
STRINGi | 9606.ENSP00000264036 |
Miscellaneous databases
RNActi | P43121, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 24 – 129 | Ig-like V-type 1Add BLAST | 106 | |
Domaini | 139 – 242 | Ig-like V-type 2Add BLAST | 104 | |
Domaini | 244 – 330 | Ig-like C2-type 1Add BLAST | 87 | |
Domaini | 335 – 424 | Ig-like C2-type 2Add BLAST | 90 | |
Domaini | 430 – 510 | Ig-like C2-type 3Add BLAST | 81 |
Keywords - Domaini
Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502QV1U, Eukaryota |
GeneTreei | ENSGT00940000155838 |
HOGENOMi | CLU_028888_3_0_1 |
InParanoidi | P43121 |
OMAi | NPQPHFS |
PhylomeDBi | P43121 |
TreeFami | TF330534 |
Family and domain databases
Gene3Di | 2.60.40.10, 5 hits |
InterProi | View protein in InterPro IPR013162, CD80_C2-set IPR007110, Ig-like_dom IPR036179, Ig-like_dom_sf IPR013783, Ig-like_fold IPR003599, Ig_sub IPR003598, Ig_sub2 IPR013106, Ig_V-set IPR013151, Immunoglobulin |
Pfami | View protein in Pfam PF08205, C2-set_2, 1 hit PF00047, ig, 1 hit PF07686, V-set, 1 hit |
SMARTi | View protein in SMART SM00409, IG, 5 hits SM00408, IGc2, 3 hits |
SUPFAMi | SSF48726, SSF48726, 5 hits |
PROSITEi | View protein in PROSITE PS50835, IG_LIKE, 5 hits |
s (2)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketIsoform 1 (identifier: P43121-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MGLPRLVCAF LLAACCCCPR VAGVPGEAEQ PAPELVEVEV GSTALLKCGL
60 70 80 90 100
SQSQGNLSHV DWFSVHKEKR TLIFRVRQGQ GQSEPGEYEQ RLSLQDRGAT
110 120 130 140 150
LALTQVTPQD ERIFLCQGKR PRSQEYRIQL RVYKAPEEPN IQVNPLGIPV
160 170 180 190 200
NSKEPEEVAT CVGRNGYPIP QVIWYKNGRP LKEEKNRVHI QSSQTVESSG
210 220 230 240 250
LYTLQSILKA QLVKEDKDAQ FYCELNYRLP SGNHMKESRE VTVPVFYPTE
260 270 280 290 300
KVWLEVEPVG MLKEGDRVEI RCLADGNPPP HFSISKQNPS TREAEEETTN
310 320 330 340 350
DNGVLVLEPA RKEHSGRYEC QGLDLDTMIS LLSEPQELLV NYVSDVRVSP
360 370 380 390 400
AAPERQEGSS LTLTCEAESS QDLEFQWLRE ETGQVLERGP VLQLHDLKRE
410 420 430 440 450
AGGGYRCVAS VPSIPGLNRT QLVNVAIFGP PWMAFKERKV WVKENMVLNL
460 470 480 490 500
SCEASGHPRP TISWNVNGTA SEQDQDPQRV LSTLNVLVTP ELLETGVECT
510 520 530 540 550
ASNDLGKNTS ILFLELVNLT TLTPDSNTTT GLSTSTASPH TRANSTSTER
560 570 580 590 600
KLPEPESRGV VIVAVIVCIL VLAVLGAVLY FLYKKGKLPC RRSGKQEITL
610 620 630 640
PPSRKSELVV EVKSDKLPEE MGLLQGSSGD KRAPGDQGEK YIDLRH
Sequence cautioni
The sequence BAD93162 differs from that shown. Reason: Erroneous initiation.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 322 – 324 | GLD → AWN (PubMed:2602381).Curated | 3 | |
Sequence conflicti | 322 – 324 | GLD → AWN (PubMed:8378324).Curated | 3 | |
Sequence conflicti | 383 | G → D (PubMed:2602381).Curated | 1 | |
Sequence conflicti | 383 | G → D (PubMed:8378324).Curated | 1 | |
Sequence conflicti | 383 | G → D (PubMed:11709656).Curated | 1 | |
Sequence conflicti | 424 – 425 | NV → KL (PubMed:2602381).Curated | 2 | |
Sequence conflicti | 424 – 425 | NV → KL (PubMed:8378324).Curated | 2 | |
Sequence conflicti | 606 | S → T (PubMed:2602381).Curated | 1 | |
Sequence conflicti | 606 | S → T (PubMed:8378324).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_049915 | 89 | E → G1 PublicationCorresponds to variant dbSNP:rs34587557Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_016938 | 1 – 187 | MGLPR…EEKNR → MVYIVRQFLLYNVSGSVYLD QLIVLLTAKFSILRIAGSRV HHSPFSGHLDGCSFLSLQHS LHTSLDMSRHENVFLGLTLS SKSAGLKGFQLAFVPGLLQG TGGYLDGPLPTPVDNPRVGL EVGLRLSLPPLPPCPG in isoform 2. 1 PublicationAdd BLAST | 187 | |
Alternative sequenceiVSP_016939 | 549 – 646 | ERKLP…IDLRH → GKPGLAREQGCARASFLPCP SPESPVQKGE in isoform 2. 1 PublicationAdd BLAST | 98 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M29277 mRNA Translation: AAA20824.1 M28882 mRNA Translation: AAA20922.1 X68264 , X68265, X68266, X68267, X68268, X68270, X68271 Genomic DNA Translation: CAA48332.1 AF089868 mRNA Translation: AAD17799.1 AK126303 mRNA Translation: BAC86520.1 AB209925 mRNA Translation: BAD93162.1 Different initiation. BC056418 mRNA Translation: AAH56418.1 |
CCDSi | CCDS31690.1 [P43121-1] |
PIRi | I38049 |
RefSeqi | NP_006491.2, NM_006500.2 [P43121-1] |
Genome annotation databases
Ensembli | ENST00000264036; ENSP00000264036; ENSG00000076706 [P43121-1] |
GeneIDi | 4162 |
KEGGi | hsa:4162 |
UCSCi | uc001pwf.4, human [P43121-1] |
Keywords - Coding sequence diversityi
Alternative splicing, PolymorphismSimilar proteinsi
Cross-referencesi
Web resourcesi
Atlas of Genetics and Cytogenetics in Oncology and Haematology |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M29277 mRNA Translation: AAA20824.1 M28882 mRNA Translation: AAA20922.1 X68264 , X68265, X68266, X68267, X68268, X68270, X68271 Genomic DNA Translation: CAA48332.1 AF089868 mRNA Translation: AAD17799.1 AK126303 mRNA Translation: BAC86520.1 AB209925 mRNA Translation: BAD93162.1 Different initiation. BC056418 mRNA Translation: AAH56418.1 |
CCDSi | CCDS31690.1 [P43121-1] |
PIRi | I38049 |
RefSeqi | NP_006491.2, NM_006500.2 [P43121-1] |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 110332, 29 interactors |
DIPi | DIP-52791N |
IntActi | P43121, 17 interactors |
MINTi | P43121 |
STRINGi | 9606.ENSP00000264036 |
Chemistry databases
ChEMBLi | CHEMBL3712863 |
GuidetoPHARMACOLOGYi | 2988 |
PTM databases
GlyConnecti | 1104, 2 N-Linked glycans (2 sites) |
GlyGeni | P43121, 9 sites |
iPTMneti | P43121 |
PhosphoSitePlusi | P43121 |
SwissPalmi | P43121 |
Polymorphism and mutation databases
BioMutai | MCAM |
DMDMi | 85681878 |
Proteomic databases
EPDi | P43121 |
jPOSTi | P43121 |
MassIVEi | P43121 |
MaxQBi | P43121 |
PaxDbi | P43121 |
PeptideAtlasi | P43121 |
PRIDEi | P43121 |
ProteomicsDBi | 55589 [P43121-1] 55590 [P43121-2] |
Protocols and materials databases
ABCDi | P43121, 2 sequenced antibodies |
Antibodypediai | 2244, 1932 antibodies |
DNASUi | 4162 |
Genome annotation databases
Ensembli | ENST00000264036; ENSP00000264036; ENSG00000076706 [P43121-1] |
GeneIDi | 4162 |
KEGGi | hsa:4162 |
UCSCi | uc001pwf.4, human [P43121-1] |
Organism-specific databases
CTDi | 4162 |
DisGeNETi | 4162 |
EuPathDBi | HostDB:ENSG00000076706.14 |
GeneCardsi | MCAM |
HGNCi | HGNC:6934, MCAM |
HPAi | ENSG00000076706, Low tissue specificity |
MIMi | 155735, gene |
neXtProti | NX_P43121 |
OpenTargetsi | ENSG00000076706 |
PharmGKBi | PA30678 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502QV1U, Eukaryota |
GeneTreei | ENSGT00940000155838 |
HOGENOMi | CLU_028888_3_0_1 |
InParanoidi | P43121 |
OMAi | NPQPHFS |
PhylomeDBi | P43121 |
TreeFami | TF330534 |
Enzyme and pathway databases
PathwayCommonsi | P43121 |
Miscellaneous databases
BioGRID-ORCSi | 4162, 4 hits in 849 CRISPR screens |
ChiTaRSi | MCAM, human |
GeneWikii | CD146 |
GenomeRNAii | 4162 |
Pharosi | P43121, Tbio |
PROi | PR:P43121 |
RNActi | P43121, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000076706, Expressed in popliteal artery and 242 other tissues |
ExpressionAtlasi | P43121, baseline and differential |
Genevisiblei | P43121, HS |
Family and domain databases
Gene3Di | 2.60.40.10, 5 hits |
InterProi | View protein in InterPro IPR013162, CD80_C2-set IPR007110, Ig-like_dom IPR036179, Ig-like_dom_sf IPR013783, Ig-like_fold IPR003599, Ig_sub IPR003598, Ig_sub2 IPR013106, Ig_V-set IPR013151, Immunoglobulin |
Pfami | View protein in Pfam PF08205, C2-set_2, 1 hit PF00047, ig, 1 hit PF07686, V-set, 1 hit |
SMARTi | View protein in SMART SM00409, IG, 5 hits SM00408, IGc2, 3 hits |
SUPFAMi | SSF48726, SSF48726, 5 hits |
PROSITEi | View protein in PROSITE PS50835, IG_LIKE, 5 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MUC18_HUMAN | |
Accessioni | P43121Primary (citable) accession number: P43121 Secondary accession number(s): O95812 Q6ZTR2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | January 10, 2006 | |
Last modified: | December 2, 2020 | |
This is version 185 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - Human chromosome 11
Human chromosome 11: entries, gene names and cross-references to MIM - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations - Human cell differentiation molecules
CD nomenclature of surface proteins of human leucocytes and list of entries