UniProtKB - P43120 (HHEX_MOUSE)
Hematopoietically-expressed homeobox protein Hhex
Hhex
Functioni
Recognizes the DNA sequence 5'-ATTAA-3' (PubMed:10804173).
Transcriptional repressor (PubMed:10804173).
Activator of WNT-mediated transcription in conjunction with CTNNB1 (PubMed:16936074).
Establishes anterior identity at two levels; acts early to enhance canonical WNT-signaling by repressing expression of TLE4, and acts later to inhibit NODAL-signaling by directly targeting NODAL (PubMed:16936074).
May play a role in hematopoietic differentiation (PubMed:8096636).
3 PublicationsRegions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 138 – 197 | HomeoboxPROSITE-ProRule annotationAdd BLAST | 60 |
GO - Molecular functioni
- chromatin binding Source: MGI
- DNA binding, bending Source: MGI
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: UniProtKB
- DNA-binding transcription factor activity Source: BHF-UCL
- DNA-binding transcription factor binding Source: MGI
- DNA-binding transcription repressor activity, RNA polymerase II-specific Source: NTNU_SB
- eukaryotic initiation factor 4E binding Source: MGI
- HMG box domain binding Source: UniProtKB
- protein homodimerization activity Source: MGI
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: MGI
- RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: NTNU_SB
- sequence-specific DNA binding Source: UniProtKB
- transcription cis-regulatory region binding Source: MGI
GO - Biological processi
- animal organ morphogenesis Source: MGI
- anterior/posterior pattern specification Source: UniProtKB
- B cell differentiation Source: BHF-UCL
- cell differentiation Source: MGI
- common bile duct development Source: MGI
- DNA conformation change Source: MGI
- embryonic heart tube development Source: MGI
- embryonic organ development Source: MGI
- endoderm development Source: MGI
- forebrain development Source: MGI
- forebrain morphogenesis Source: MGI
- gall bladder development Source: MGI
- hemopoiesis Source: MGI
- hepatic duct development Source: MGI
- hepatoblast differentiation Source: MGI
- hepatocyte differentiation Source: MGI
- interkinetic nuclear migration Source: MGI
- in utero embryonic development Source: MGI
- liver development Source: MGI
- morphogenesis of an epithelium Source: MGI
- mRNA export from nucleus Source: MGI
- multicellular organism growth Source: MGI
- myeloid leukocyte differentiation Source: MGI
- negative regulation of angiogenesis Source: BHF-UCL
- negative regulation of cyclin-dependent protein serine/threonine kinase activity Source: MGI
- negative regulation of transcription, DNA-templated Source: UniProtKB
- negative regulation of transcription by RNA polymerase II Source: UniProtKB
- negative regulation of vascular endothelial growth factor receptor signaling pathway Source: BHF-UCL
- Notch signaling pathway Source: MGI
- pancreas development Source: MGI
- poly(A)+ mRNA export from nucleus Source: MGI
- positive regulation of canonical Wnt signaling pathway Source: UniProtKB
- positive regulation of transcription, DNA-templated Source: UniProtKB
- positive regulation of transcription by RNA polymerase II Source: UniProtKB
- positive regulation of Wnt signaling pathway Source: UniProtKB
- primary lung bud formation Source: MGI
- primitive streak formation Source: MGI
- protein localization to nucleus Source: MGI
- regulation of cell population proliferation Source: MGI
- regulation of leukocyte proliferation Source: MGI
- regulation of transcription by RNA polymerase II Source: MGI
- response to peptide hormone Source: Ensembl
- response to wounding Source: Ensembl
- signal transduction Source: MGI
- thyroid gland development Source: MGI
- tissue morphogenesis Source: MGI
- vasculogenesis Source: MGI
- Wnt signaling pathway Source: UniProtKB-KW
Keywordsi
Molecular function | Developmental protein, DNA-binding, Repressor |
Biological process | Differentiation, Transcription, Transcription regulation, Wnt signaling pathway |
Names & Taxonomyi
Protein namesi | Recommended name: Hematopoietically-expressed homeobox protein HhexShort name: Homeobox protein HEX Short name: mHex Alternative name(s): Homeobox protein PRH |
Gene namesi | Name:Hhex Synonyms:Prh, Prhx |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:96086, Hhex |
VEuPathDBi | HostDB:ENSMUSG00000024986 |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
Nucleus
- nucleus Source: MGI
Other locations
- cytoplasm Source: MGI
- protein-DNA complex Source: MGI
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000049075 | 1 – 271 | Hematopoietically-expressed homeobox protein HhexAdd BLAST | 271 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 54 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P43120 |
PaxDbi | P43120 |
PRIDEi | P43120 |
ProteomicsDBi | 273338 |
PTM databases
iPTMneti | P43120 |
PhosphoSitePlusi | P43120 |
Expressioni
Developmental stagei
Gene expression databases
Bgeei | ENSMUSG00000024986, Expressed in blood and 264 other tissues |
ExpressionAtlasi | P43120, baseline and differential |
Genevisiblei | P43120, MM |
Interactioni
Subunit structurei
Interacts with CD81; the interaction prevents nuclear translocation of HHEX (PubMed:23665349).
Interacts (via N-terminus) with SOX13; abolishes the SOX13-mediated inhibition of WNT-mediated transcriptional activity via competitive inhibition of the SOX13-TCF7 complex (PubMed:20028982).
2 PublicationsGO - Molecular functioni
- DNA-binding transcription factor binding Source: MGI
- eukaryotic initiation factor 4E binding Source: MGI
- HMG box domain binding Source: UniProtKB
- protein homodimerization activity Source: MGI
Protein-protein interaction databases
BioGRIDi | 200297, 7 interactors |
IntActi | P43120, 2 interactors |
STRINGi | 10090.ENSMUSP00000025944 |
Miscellaneous databases
RNActi | P43120, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 138 | Interaction with SOX13By similarityAdd BLAST | 138 | |
Regioni | 138 – 271 | Required for WNT signaling inductionBy similarityAdd BLAST | 134 | |
Regioni | 195 – 271 | DisorderedSequence analysisAdd BLAST | 77 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 195 – 212 | Basic and acidic residuesSequence analysisAdd BLAST | 18 | |
Compositional biasi | 213 – 246 | Polar residuesSequence analysisAdd BLAST | 34 |
Keywords - Domaini
HomeoboxPhylogenomic databases
eggNOGi | KOG0483, Eukaryota |
GeneTreei | ENSGT00940000164435 |
HOGENOMi | CLU_081944_0_0_1 |
InParanoidi | P43120 |
OMAi | SVNDYTH |
OrthoDBi | 1596695at2759 |
PhylomeDBi | P43120 |
TreeFami | TF325047 |
Family and domain databases
CDDi | cd00086, homeodomain, 1 hit |
InterProi | View protein in InterPro IPR009057, Homeobox-like_sf IPR017970, Homeobox_CS IPR001356, Homeobox_dom IPR020479, Homeobox_metazoa |
Pfami | View protein in Pfam PF00046, Homeodomain, 1 hit |
PRINTSi | PR00024, HOMEOBOX |
SMARTi | View protein in SMART SM00389, HOX, 1 hit |
SUPFAMi | SSF46689, SSF46689, 1 hit |
PROSITEi | View protein in PROSITE PS00027, HOMEOBOX_1, 1 hit PS50071, HOMEOBOX_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MQFPHPGPAA APAVGVPLYA PTPLLQPAHP TPFYIDDILG RGPAAPTPTP
60 70 80 90 100
TLPSPNSSFT SLVSSYRTPV YEPTPVHPAF SHHPAAALAA AYGPSGFGGP
110 120 130 140 150
LYPFPRTVND YTHALLRHDP LGKPLLWSPF LQRPLHKRKG GQVRFSNDQT
160 170 180 190 200
VELEKKFETQ KYLSPPERKR LAKMLQLSER QVKTWFQNRR AKWRRLKQEN
210 220 230 240 250
PQSNKKDALD SLDTSCEQGQ DLPSEQNKGA SLDRSQCSPS PASQEDPDSE
260 270
ISEDSDQEVD IEGDKGYFNA G
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketG3UXH1 | G3UXH1_MOUSE | Hematopoietically-expressed homeobo... | Hhex | 98 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 10 | A → L in BAB29163 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 33 | F → L in BAA76714 (PubMed:10101294).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z21524 Genomic DNA Translation: CAA79729.1 AB017132 Genomic DNA Translation: BAA76714.1 AF132550 Genomic DNA Translation: AAF04349.1 AK014111 mRNA Translation: BAB29163.1 Frameshift. AK033664 mRNA Translation: BAC28416.1 BC057986 mRNA Translation: AAH57986.1 |
CCDSi | CCDS29778.1 |
PIRi | S30230 |
RefSeqi | NP_032271.1, NM_008245.3 |
Genome annotation databases
Ensembli | ENSMUST00000025944; ENSMUSP00000025944; ENSMUSG00000024986 |
GeneIDi | 15242 |
KEGGi | mmu:15242 |
UCSCi | uc008hio.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z21524 Genomic DNA Translation: CAA79729.1 AB017132 Genomic DNA Translation: BAA76714.1 AF132550 Genomic DNA Translation: AAF04349.1 AK014111 mRNA Translation: BAB29163.1 Frameshift. AK033664 mRNA Translation: BAC28416.1 BC057986 mRNA Translation: AAH57986.1 |
CCDSi | CCDS29778.1 |
PIRi | S30230 |
RefSeqi | NP_032271.1, NM_008245.3 |
3D structure databases
SMRi | P43120 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 200297, 7 interactors |
IntActi | P43120, 2 interactors |
STRINGi | 10090.ENSMUSP00000025944 |
PTM databases
iPTMneti | P43120 |
PhosphoSitePlusi | P43120 |
Proteomic databases
MaxQBi | P43120 |
PaxDbi | P43120 |
PRIDEi | P43120 |
ProteomicsDBi | 273338 |
Protocols and materials databases
DNASUi | 15242 |
Genome annotation databases
Ensembli | ENSMUST00000025944; ENSMUSP00000025944; ENSMUSG00000024986 |
GeneIDi | 15242 |
KEGGi | mmu:15242 |
UCSCi | uc008hio.1, mouse |
Organism-specific databases
CTDi | 3087 |
MGIi | MGI:96086, Hhex |
VEuPathDBi | HostDB:ENSMUSG00000024986 |
Phylogenomic databases
eggNOGi | KOG0483, Eukaryota |
GeneTreei | ENSGT00940000164435 |
HOGENOMi | CLU_081944_0_0_1 |
InParanoidi | P43120 |
OMAi | SVNDYTH |
OrthoDBi | 1596695at2759 |
PhylomeDBi | P43120 |
TreeFami | TF325047 |
Miscellaneous databases
BioGRID-ORCSi | 15242, 2 hits in 64 CRISPR screens |
ChiTaRSi | Ccdc39, mouse |
PROi | PR:P43120 |
RNActi | P43120, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000024986, Expressed in blood and 264 other tissues |
ExpressionAtlasi | P43120, baseline and differential |
Genevisiblei | P43120, MM |
Family and domain databases
CDDi | cd00086, homeodomain, 1 hit |
InterProi | View protein in InterPro IPR009057, Homeobox-like_sf IPR017970, Homeobox_CS IPR001356, Homeobox_dom IPR020479, Homeobox_metazoa |
Pfami | View protein in Pfam PF00046, Homeodomain, 1 hit |
PRINTSi | PR00024, HOMEOBOX |
SMARTi | View protein in SMART SM00389, HOX, 1 hit |
SUPFAMi | SSF46689, SSF46689, 1 hit |
PROSITEi | View protein in PROSITE PS00027, HOMEOBOX_1, 1 hit PS50071, HOMEOBOX_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | HHEX_MOUSE | |
Accessioni | P43120Primary (citable) accession number: P43120 Secondary accession number(s): Q544N6, Q9CRV1, Q9R1X2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | November 1, 1995 | |
Last modified: | February 23, 2022 | |
This is version 169 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot