UniProtKB - P43114 (PE2R4_RAT)
Protein
Prostaglandin E2 receptor EP4 subtype
Gene
Ptger4
Organism
Rattus norvegicus (Rat)
Status
Functioni
Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. Has a relaxing effect on smooth muscle. May play an important role in regulating renal hemodynamics, intestinal epithelial transport, adrenal aldosterone secretion, and uterine function.
Caution
Was originally designated as the EP2 subtype.Curated
GO - Molecular functioni
- prostaglandin E receptor activity Source: RGD
GO - Biological processi
- adenylate cyclase-activating G protein-coupled receptor signaling pathway Source: GO_Central
- adenylate cyclase-modulating G protein-coupled receptor signaling pathway Source: UniProtKB
- bone development Source: UniProtKB
- cellular response to glucose stimulus Source: RGD
- cellular response to interleukin-1 Source: RGD
- cellular response to mechanical stimulus Source: UniProtKB
- cellular response to prostaglandin E stimulus Source: RGD
- chemokinesis Source: RGD
- ductus arteriosus closure Source: RGD
- ERK1 and ERK2 cascade Source: UniProtKB
- female pregnancy Source: RGD
- immune response Source: RGD
- inflammatory response Source: GO_Central
- JNK cascade Source: UniProtKB
- maternal process involved in parturition Source: RGD
- negative regulation of circadian sleep/wake cycle, REM sleep Source: RGD
- negative regulation of cytokine secretion Source: UniProtKB
- negative regulation of ductus arteriosus closure Source: RGD
- negative regulation of endothelin production Source: RGD
- negative regulation of eosinophil extravasation Source: UniProtKB
- negative regulation of hydrogen peroxide metabolic process Source: RGD
- negative regulation of inflammatory response Source: UniProtKB
- negative regulation of integrin activation Source: UniProtKB
- negative regulation of interleukin-1 alpha production Source: RGD
- negative regulation of nitric-oxide synthase biosynthetic process Source: RGD
- negative regulation of peptide secretion Source: RGD
- negative regulation of small intestine smooth muscle contraction Source: RGD
- negative regulation of tumor necrosis factor production Source: RGD
- negative regulation of tumor necrosis factor secretion Source: RGD
- positive regulation of antral ovarian follicle growth Source: RGD
- positive regulation of bone resorption Source: RGD
- positive regulation of calcitonin secretion Source: RGD
- positive regulation of cAMP-mediated signaling Source: RGD
- positive regulation of cell adhesion Source: RGD
- positive regulation of cell population proliferation Source: RGD
- positive regulation of chemokinesis Source: RGD
- positive regulation of circadian sleep/wake cycle, non-REM sleep Source: RGD
- positive regulation of cytokine secretion Source: UniProtKB
- positive regulation of cytosolic calcium ion concentration Source: GO_Central
- positive regulation of gene expression Source: RGD
- positive regulation of hyaluronan biosynthetic process Source: RGD
- positive regulation of inflammatory response Source: UniProtKB
- positive regulation of interleukin-10 production Source: RGD
- positive regulation of interleukin-8 production Source: RGD
- positive regulation of matrix metallopeptidase secretion Source: RGD
- positive regulation of mucus secretion Source: RGD
- positive regulation of neutrophil extravasation Source: RGD
- positive regulation of ossification Source: RGD
- positive regulation of osteoblast differentiation Source: RGD
- positive regulation of ovarian follicle development Source: RGD
- positive regulation of protein phosphorylation Source: RGD
- positive regulation of protein secretion Source: RGD
- positive regulation of renal sodium excretion Source: RGD
- positive regulation of smooth muscle cell migration Source: RGD
- positive regulation of substance P secretion Source: RGD
- positive regulation of substance P secretion, neurotransmission Source: RGD
- positive regulation of tyrosine phosphorylation of STAT protein Source: RGD
- positive regulation of urine volume Source: RGD
- positive regulation of wound healing Source: RGD
- regulation of circadian sleep/wake cycle, wakefulness Source: RGD
- regulation of ossification Source: RGD
- regulation of stress fiber assembly Source: UniProtKB
- response to amino acid Source: RGD
- response to drug Source: RGD
- response to interleukin-1 Source: RGD
- response to lipopolysaccharide Source: RGD
- response to mechanical stimulus Source: RGD
- response to organic cyclic compound Source: RGD
- response to progesterone Source: RGD
- response to prostaglandin E Source: RGD
- response to salt Source: RGD
- response to water-immersion restraint stress Source: RGD
- T-helper cell differentiation Source: UniProtKB
Keywordsi
Molecular function | G-protein coupled receptor, Receptor, Transducer |
Enzyme and pathway databases
Reactomei | R-RNO-391908, Prostanoid ligand receptors |
Names & Taxonomyi
Protein namesi | Recommended name: Prostaglandin E2 receptor EP4 subtypeShort name: PGE receptor EP4 subtype Short name: PGE2 receptor EP4 subtype Alternative name(s): Prostanoid EP4 receptor |
Gene namesi | Name:Ptger4 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 628641, Ptger4 |
Subcellular locationi
Plasma membrane
Nucleus
- nuclear membrane Source: RGD
Plasma Membrane
- plasma membrane Source: RGD
Other locations
- integral component of membrane Source: UniProtKB-KW
- neuron projection terminus Source: RGD
- neuronal cell body Source: RGD
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 19 | ExtracellularSequence analysisAdd BLAST | 19 | |
Transmembranei | 20 – 43 | Helical; Name=1Sequence analysisAdd BLAST | 24 | |
Topological domaini | 44 – 55 | CytoplasmicSequence analysisAdd BLAST | 12 | |
Transmembranei | 56 – 79 | Helical; Name=2Sequence analysisAdd BLAST | 24 | |
Topological domaini | 80 – 96 | ExtracellularSequence analysisAdd BLAST | 17 | |
Transmembranei | 97 – 115 | Helical; Name=3Sequence analysisAdd BLAST | 19 | |
Topological domaini | 116 – 135 | CytoplasmicSequence analysisAdd BLAST | 20 | |
Transmembranei | 136 – 160 | Helical; Name=4Sequence analysisAdd BLAST | 25 | |
Topological domaini | 161 – 184 | ExtracellularSequence analysisAdd BLAST | 24 | |
Transmembranei | 185 – 211 | Helical; Name=5Sequence analysisAdd BLAST | 27 | |
Topological domaini | 212 – 270 | CytoplasmicSequence analysisAdd BLAST | 59 | |
Transmembranei | 271 – 298 | Helical; Name=6Sequence analysisAdd BLAST | 28 | |
Topological domaini | 299 – 315 | ExtracellularSequence analysisAdd BLAST | 17 | |
Transmembranei | 316 – 335 | Helical; Name=7Sequence analysisAdd BLAST | 20 | |
Topological domaini | 336 – 488 | CytoplasmicSequence analysisAdd BLAST | 153 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Chemistry databases
ChEMBLi | CHEMBL4086 |
DrugCentrali | P43114 |
GuidetoPHARMACOLOGYi | 343 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000070068 | 1 – 488 | Prostaglandin E2 receptor EP4 subtypeAdd BLAST | 488 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 7 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 92 ↔ 170 | PROSITE-ProRule annotation | ||
Modified residuei | 377 | PhosphoserineBy similarity | 1 | |
Modified residuei | 380 | PhosphoserineBy similarity | 1 | |
Modified residuei | 382 | PhosphoserineBy similarity | 1 | |
Modified residuei | 385 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Phosphorylation mediates agonist-mediated desensitization by promoting cytoplasmic retention.By similarity
Keywords - PTMi
Disulfide bond, Glycoprotein, PhosphoproteinProteomic databases
PaxDbi | P43114 |
PTM databases
GlyGeni | P43114, 1 site |
PhosphoSitePlusi | P43114 |
Interactioni
Subunit structurei
Interacts with FEM1A.
By similarityProtein-protein interaction databases
STRINGi | 10116.ENSRNOP00000017886 |
Chemistry databases
BindingDBi | P43114 |
Family & Domainsi
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 230 – 235 | Poly-Ala | 6 |
Sequence similaritiesi
Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3656, Eukaryota |
InParanoidi | P43114 |
OrthoDBi | 972015at2759 |
PhylomeDBi | P43114 |
TreeFami | TF324982 |
Family and domain databases
InterProi | View protein in InterPro IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM IPR001758, Prost_EP4_rcpt IPR008365, Prostanoid_rcpt IPR001244, Prostglndn_DP_rcpt |
PANTHERi | PTHR11866, PTHR11866, 1 hit PTHR11866:SF6, PTHR11866:SF6, 1 hit |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit |
PRINTSi | PR00237, GPCRRHODOPSN PR00428, PROSTAGLNDNR PR01788, PROSTANOIDR PR00586, PRSTNOIDEP4R |
PROSITEi | View protein in PROSITE PS00237, G_PROTEIN_RECEP_F1_1, 1 hit PS50262, G_PROTEIN_RECEP_F1_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
P43114-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSIPGVNASF SSTPERLNSP VTIPAVMFIF GVVGNLVAIV VLCKSRKEQK
60 70 80 90 100
ETTFYTLVCG LAVTDLLGTL LVSPVTIATY MKGQWPGDQA LCDYSTFILL
110 120 130 140 150
FFGLSGLSII CAMSIERYLA INHAYFYSHY VDKRLAGLTL FAVYASNVLF
160 170 180 190 200
CALPNMGLGR SERQYPGTWC FIDWTTNVTA YAAFSYMYAG FSSFLILATV
210 220 230 240 250
LCNVLVCGAL LRMLRQFMRR TSLGTEQHHA AAAAAVASVA CRGHAAASPA
260 270 280 290 300
LQRLSDFRRR RSFRRIAGAE IQMVILLIAT SLVVLICSIP LVVRVFINQL
310 320 330 340 350
YQPSVVKDIS RNPDLQAIRI ASVNPILDPW IYILLRKTVL SKAIEKIKCL
360 370 380 390 400
FCRIGGSGRD GSAQHCSESR RTSSAMSGHS RSFLSRELRE ISSTSHTLLY
410 420 430 440 450
LPDLTESSLG GKNLLPGTHG MGLTQADTTS LRTLRISETS DSSQGQDSES
460 470 480
VLLVDEVSGS QREEPASKGN SLQVTFPSET LKLSEKCI
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A0G2JSM6 | A0A0G2JSM6_RAT | Prostaglandin E2 receptor EP4 subty... | Ptger4 rCG_51033 | 488 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 214 | L → H in AAB53326 (PubMed:9537820).Curated | 1 | |
Sequence conflicti | 396 | H → Q in AAB53326 (PubMed:9537820).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D28860 mRNA Translation: BAA06011.1 U94709 mRNA Translation: AAB53326.1 |
PIRi | JC2241 |
RefSeqi | NP_114465.3, NM_032076.3 XP_006232058.1, XM_006231996.3 |
Genome annotation databases
GeneIDi | 84023 |
KEGGi | rno:84023 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D28860 mRNA Translation: BAA06011.1 U94709 mRNA Translation: AAB53326.1 |
PIRi | JC2241 |
RefSeqi | NP_114465.3, NM_032076.3 XP_006232058.1, XM_006231996.3 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000017886 |
Chemistry databases
BindingDBi | P43114 |
ChEMBLi | CHEMBL4086 |
DrugCentrali | P43114 |
GuidetoPHARMACOLOGYi | 343 |
Protein family/group databases
GPCRDBi | Search... |
PTM databases
GlyGeni | P43114, 1 site |
PhosphoSitePlusi | P43114 |
Proteomic databases
PaxDbi | P43114 |
Genome annotation databases
GeneIDi | 84023 |
KEGGi | rno:84023 |
Organism-specific databases
CTDi | 5734 |
RGDi | 628641, Ptger4 |
Phylogenomic databases
eggNOGi | KOG3656, Eukaryota |
InParanoidi | P43114 |
OrthoDBi | 972015at2759 |
PhylomeDBi | P43114 |
TreeFami | TF324982 |
Enzyme and pathway databases
Reactomei | R-RNO-391908, Prostanoid ligand receptors |
Miscellaneous databases
PROi | PR:P43114 |
Family and domain databases
InterProi | View protein in InterPro IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM IPR001758, Prost_EP4_rcpt IPR008365, Prostanoid_rcpt IPR001244, Prostglndn_DP_rcpt |
PANTHERi | PTHR11866, PTHR11866, 1 hit PTHR11866:SF6, PTHR11866:SF6, 1 hit |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit |
PRINTSi | PR00237, GPCRRHODOPSN PR00428, PROSTAGLNDNR PR01788, PROSTANOIDR PR00586, PRSTNOIDEP4R |
PROSITEi | View protein in PROSITE PS00237, G_PROTEIN_RECEP_F1_1, 1 hit PS50262, G_PROTEIN_RECEP_F1_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PE2R4_RAT | |
Accessioni | P43114Primary (citable) accession number: P43114 Secondary accession number(s): O08728 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | November 1, 1995 | |
Last modified: | December 2, 2020 | |
This is version 143 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - 7-transmembrane G-linked receptors
List of 7-transmembrane G-linked receptor entries