UniProtKB - P43081 (GUC1A_MOUSE)
Guanylyl cyclase-activating protein 1
Guca1a
Functioni
Stimulates retinal guanylyl cyclase when free calcium ions concentration is low and inhibits guanylyl cyclase when free calcium ions concentration is elevated (By similarity).
This Ca2+-sensitive regulation of retinal guanylyl cyclase is a key event in recovery of the dark state of rod photoreceptors following light exposure (By similarity).
May be involved in cone photoreceptor light response and recovery of response in bright light (PubMed:25673692).
By similarity1 PublicationSites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 64 | Calcium 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 66 | Calcium 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 68 | Calcium 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 70 | Calcium 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 75 | Calcium 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 100 | Calcium 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 102 | Calcium 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 104 | Calcium 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 106 | Calcium 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 111 | Calcium 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 144 | Calcium 3PROSITE-ProRule annotation | 1 | |
Metal bindingi | 146 | Calcium 3PROSITE-ProRule annotation | 1 | |
Metal bindingi | 148 | Calcium 3PROSITE-ProRule annotation | 1 | |
Metal bindingi | 150 | Calcium 3PROSITE-ProRule annotation | 1 | |
Metal bindingi | 155 | Calcium 3PROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- calcium ion binding Source: UniProtKB
- calcium sensitive guanylate cyclase activator activity Source: MGI
- guanylate cyclase regulator activity Source: MGI
GO - Biological processi
- cellular response to calcium ion Source: UniProtKB
- phototransduction Source: MGI
- positive regulation of cGMP-mediated signaling Source: MGI
- positive regulation of guanylate cyclase activity Source: MGI
- regulation of guanylate cyclase activity Source: MGI
- visual perception Source: MGI
Keywordsi
Biological process | Sensory transduction, Vision |
Ligand | Calcium, Metal-binding |
Enzyme and pathway databases
Reactomei | R-MMU-2514859, Inactivation, recovery and regulation of the phototransduction cascade |
Names & Taxonomyi
Protein namesi | Recommended name: Guanylyl cyclase-activating protein 1Short name: GCAP 1 Alternative name(s): Guanylate cyclase activator 1A |
Gene namesi | Name:Guca1a Synonyms:Gcap, Gcap1, Guca1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:102770, Guca1a |
VEuPathDBi | HostDB:ENSMUSG00000023982 |
Subcellular locationi
Other locations
- Membrane ; Lipid-anchor By similarity
- Photoreceptor inner segment 1 Publication
- photoreceptor outer segment 2 Publications
Note: Subcellular location is not affected by light or dark conditions.
Other locations
- cone photoreceptor outer segment Source: UniProtKB
- membrane Source: MGI
- photoreceptor inner segment Source: UniProtKB
- photoreceptor outer segment Source: MGI
Keywords - Cellular componenti
Cell projection, MembranePathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedSequence analysis | |||
ChainiPRO_0000073804 | 2 – 202 | Guanylyl cyclase-activating protein 1Add BLAST | 201 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 2 | N-myristoyl glycineBy similarity | 1 | |
Modified residuei | 3 | Deamidated asparagineSequence analysis | 1 |
Keywords - PTMi
Lipoprotein, MyristateProteomic databases
PaxDbi | P43081 |
PRIDEi | P43081 |
ProteomicsDBi | 271490 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000023982, Expressed in retina and 98 other tissues |
Genevisiblei | P43081, MM |
Interactioni
Subunit structurei
Homodimer.
By similarityProtein-protein interaction databases
STRINGi | 10090.ENSMUSP00000060027 |
Miscellaneous databases
RNActi | P43081, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 31 – 49 | EF-hand 1PROSITE-ProRule annotationAdd BLAST | 19 | |
Domaini | 51 – 86 | EF-hand 2PROSITE-ProRule annotationAdd BLAST | 36 | |
Domaini | 87 – 122 | EF-hand 3PROSITE-ProRule annotationAdd BLAST | 36 | |
Domaini | 131 – 166 | EF-hand 4PROSITE-ProRule annotationAdd BLAST | 36 |
Domaini
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG0044, Eukaryota |
GeneTreei | ENSGT00940000160607 |
HOGENOMi | CLU_072366_4_1_1 |
InParanoidi | P43081 |
OMAi | IRTINPC |
OrthoDBi | 1271942at2759 |
PhylomeDBi | P43081 |
TreeFami | TF333971 |
Family and domain databases
CDDi | cd00051, EFh, 2 hits |
InterProi | View protein in InterPro IPR011992, EF-hand-dom_pair IPR018247, EF_Hand_1_Ca_BS IPR002048, EF_hand_dom IPR033022, GUCA1A IPR028846, Recoverin |
PANTHERi | PTHR23055, PTHR23055, 1 hit PTHR23055:SF13, PTHR23055:SF13, 1 hit |
Pfami | View protein in Pfam PF13499, EF-hand_7, 1 hit PF13833, EF-hand_8, 1 hit |
SMARTi | View protein in SMART SM00054, EFh, 3 hits |
SUPFAMi | SSF47473, SSF47473, 1 hit |
PROSITEi | View protein in PROSITE PS00018, EF_HAND_1, 3 hits PS50222, EF_HAND_2, 4 hits |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MGNIMEGKSV EELSSTECHQ WYKKFMTECP SGQLTLYEFR QFFGLKNLSP
60 70 80 90 100
SASQYVEQMF ETFDFNKDGY IDFMEYVAAL SLVLKGKVEQ KLRWYFKLYD
110 120 130 140 150
VDGNGCIDRD ELLTIIRAIR TINPWSDSSM SAEEFTDTVF AKIDINGDGE
160 170 180 190 200
LSLEEFMEGV QKDQMLLDTL TRSLDLTGIV RRLQNGEHEE AGTGDLAAEA
AG
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 4 | I → V in AAA60716 (PubMed:7983048).Curated | 1 | |
Sequence conflicti | 29 | C → V in AAA60716 (PubMed:7983048).Curated | 1 | |
Sequence conflicti | 194 | G → S in AAA60716 (PubMed:7983048).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L36860 mRNA Translation: AAA60716.1 AC112683 Genomic DNA No translation available. CH466559 Genomic DNA Translation: EDL23558.1 CH466559 Genomic DNA Translation: EDL23559.1 CH466559 Genomic DNA Translation: EDL23560.1 BC026834 mRNA Translation: AAH26834.1 BC031810 mRNA Translation: AAH31810.1 |
CCDSi | CCDS28848.1 |
PIRi | B55331 |
RefSeqi | NP_032215.2, NM_008189.3 XP_011244598.1, XM_011246296.2 |
Genome annotation databases
Ensembli | ENSMUST00000059348; ENSMUSP00000060027; ENSMUSG00000023982 |
GeneIDi | 14913 |
KEGGi | mmu:14913 |
UCSCi | uc008cvc.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L36860 mRNA Translation: AAA60716.1 AC112683 Genomic DNA No translation available. CH466559 Genomic DNA Translation: EDL23558.1 CH466559 Genomic DNA Translation: EDL23559.1 CH466559 Genomic DNA Translation: EDL23560.1 BC026834 mRNA Translation: AAH26834.1 BC031810 mRNA Translation: AAH31810.1 |
CCDSi | CCDS28848.1 |
PIRi | B55331 |
RefSeqi | NP_032215.2, NM_008189.3 XP_011244598.1, XM_011246296.2 |
3D structure databases
AlphaFoldDBi | P43081 |
SMRi | P43081 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000060027 |
Proteomic databases
PaxDbi | P43081 |
PRIDEi | P43081 |
ProteomicsDBi | 271490 |
Protocols and materials databases
Antibodypediai | 30136, 191 antibodies from 29 providers |
DNASUi | 14913 |
Genome annotation databases
Ensembli | ENSMUST00000059348; ENSMUSP00000060027; ENSMUSG00000023982 |
GeneIDi | 14913 |
KEGGi | mmu:14913 |
UCSCi | uc008cvc.1, mouse |
Organism-specific databases
CTDi | 2978 |
MGIi | MGI:102770, Guca1a |
VEuPathDBi | HostDB:ENSMUSG00000023982 |
Phylogenomic databases
eggNOGi | KOG0044, Eukaryota |
GeneTreei | ENSGT00940000160607 |
HOGENOMi | CLU_072366_4_1_1 |
InParanoidi | P43081 |
OMAi | IRTINPC |
OrthoDBi | 1271942at2759 |
PhylomeDBi | P43081 |
TreeFami | TF333971 |
Enzyme and pathway databases
Reactomei | R-MMU-2514859, Inactivation, recovery and regulation of the phototransduction cascade |
Miscellaneous databases
BioGRID-ORCSi | 14913, 2 hits in 76 CRISPR screens |
ChiTaRSi | Guca1a, mouse |
PROi | PR:P43081 |
RNActi | P43081, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000023982, Expressed in retina and 98 other tissues |
Genevisiblei | P43081, MM |
Family and domain databases
CDDi | cd00051, EFh, 2 hits |
InterProi | View protein in InterPro IPR011992, EF-hand-dom_pair IPR018247, EF_Hand_1_Ca_BS IPR002048, EF_hand_dom IPR033022, GUCA1A IPR028846, Recoverin |
PANTHERi | PTHR23055, PTHR23055, 1 hit PTHR23055:SF13, PTHR23055:SF13, 1 hit |
Pfami | View protein in Pfam PF13499, EF-hand_7, 1 hit PF13833, EF-hand_8, 1 hit |
SMARTi | View protein in SMART SM00054, EFh, 3 hits |
SUPFAMi | SSF47473, SSF47473, 1 hit |
PROSITEi | View protein in PROSITE PS00018, EF_HAND_1, 3 hits PS50222, EF_HAND_2, 4 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | GUC1A_MOUSE | |
Accessioni | P43081Primary (citable) accession number: P43081 Secondary accession number(s): Q8R0H3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | October 3, 2012 | |
Last modified: | May 25, 2022 | |
This is version 163 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot