Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Entry version 184 (13 Nov 2019)
Sequence version 1 (01 Nov 1995)
Previous versions | rss
Help videoAdd a publicationFeedback
Protein

Excitatory amino acid transporter 1

Gene

SLC1A3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate (PubMed:7521911, PubMed:8123008, PubMed:20477940, PubMed:26690923, PubMed:28032905, PubMed:28424515). Functions as a symporter that transports one amino acid molecule together with two or three Na+ ions and one proton, in parallel with the counter-transport of one K+ ion (PubMed:20477940). Mediates Cl- flux that is not coupled to amino acid transport; this avoids the accumulation of negative charges due to aspartate and Na+ symport (PubMed:20477940). Plays a redundant role in the rapid removal of released glutamate from the synaptic cleft, which is essential for terminating the postsynaptic action of glutamate (By similarity).By similarity6 Publications

<p>This subsection of the ‘Function’ section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

  1. KM=525 µM for L-glutamate1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the ‘Description’ field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi394Sodium 1; via carbonyl oxygenBy similarity1
    Metal bindingi396Sodium 2; via carbonyl oxygenCombined sources1 Publication1
    Metal bindingi398Sodium 1By similarity1
    <p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei402AspartateCombined sources1 Publication1
    Binding sitei476AspartateCombined sources1 Publication1
    Metal bindingi483Sodium 1; via carbonyl oxygenBy similarity1
    Binding sitei483AspartateCombined sources1 Publication1
    Metal bindingi487Sodium 1By similarity1

    <p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

    GO - Biological processi

    <p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

    Biological processAmino-acid transport, Symport, Transport
    LigandChloride, Metal-binding, Potassium, Sodium

    Enzyme and pathway databases

    Reactome - a knowledgebase of biological pathways and processes

    More...
    Reactomei
    R-HSA-210455 Astrocytic Glutamate-Glutamine Uptake And Metabolism
    R-HSA-210500 Glutamate Neurotransmitter Release Cycle
    R-HSA-425393 Transport of inorganic cations/anions and amino acids/oligopeptides
    R-HSA-5619062 Defective SLC1A3 causes episodic ataxia 6 (EA6)

    Protein family/group databases

    Transport Classification Database

    More...
    TCDBi
    2.A.23.2.6 the dicarboxylate/amino acid:cation (na(+) or h(+)) symporter (daacs) family

    <p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
    Recommended name:
    Excitatory amino acid transporter 12 Publications
    Alternative name(s):
    Sodium-dependent glutamate/aspartate transporter 11 Publication
    Short name:
    GLAST-11 Publication
    Solute carrier family 1 member 3
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
    Name:SLC1A3
    Synonyms:EAAT12 Publications, GLAST, GLAST11 Publication
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiHomo sapiens (Human)
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri9606 [NCBI]
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
    • UP000005640 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 5

    Organism-specific databases

    Human Gene Nomenclature Database

    More...
    HGNCi
    HGNC:10941 SLC1A3

    Online Mendelian Inheritance in Man (OMIM)

    More...
    MIMi
    600111 gene

    neXtProt; the human protein knowledge platform

    More...
    neXtProti
    NX_P43003

    <p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/help/subcellular_location_section">'Subcellular location'</a> section describes the subcellular compartment where each non-membrane region of a membrane-spanning protein is found.<p><a href='/help/topo_dom' target='_top'>More...</a></p>Topological domaini1 – 47Cytoplasmic1 PublicationAdd BLAST47
    <p>This subsection of the <a href="http://www.uniprot.org/help/subcellular_location_section">'Subcellular location'</a> section describes the extent of a membrane-spanning region of the protein. It denotes the presence of both alpha-helical transmembrane regions and the membrane spanning regions of beta-barrel transmembrane proteins.<p><a href='/help/transmem' target='_top'>More...</a></p>Transmembranei48 – 68Helical; Name=11 PublicationAdd BLAST21
    Topological domaini69 – 86Extracellular1 PublicationAdd BLAST18
    Transmembranei87 – 108Helical; Name=21 PublicationAdd BLAST22
    Topological domaini109 – 122Cytoplasmic1 PublicationAdd BLAST14
    Transmembranei123 – 145Helical; Name=31 PublicationAdd BLAST23
    Topological domaini146 – 236Extracellular1 PublicationAdd BLAST91
    Transmembranei237 – 260Helical; Name=41 PublicationAdd BLAST24
    Topological domaini261 – 269Cytoplasmic1 Publication9
    Transmembranei270 – 297Helical; Name=51 PublicationAdd BLAST28
    Topological domaini298 – 318Extracellular1 PublicationAdd BLAST21
    Transmembranei319 – 340Helical; Name=61 PublicationAdd BLAST22
    Topological domaini341 – 345Cytoplasmic1 Publication5
    <p>This subsection of the <a href="http://www.uniprot.org/help/subcellular_location_section">'Subcellular location'</a> section describes the extent of a region that is buried within a membrane, but does not cross it.<p><a href='/help/intramem' target='_top'>More...</a></p>Intramembranei346 – 376Discontinuously helical1 PublicationAdd BLAST31
    Topological domaini377 – 385Cytoplasmic1 Publication9
    Transmembranei386 – 412Helical; Name=71 PublicationAdd BLAST27
    Topological domaini413 – 425Extracellular1 PublicationAdd BLAST13
    Intramembranei426 – 459Discontinuously helical1 PublicationAdd BLAST34
    Topological domaini460 – 472Extracellular1 PublicationAdd BLAST13
    Transmembranei473 – 494Helical; Name=81 PublicationAdd BLAST22
    Topological domaini495 – 542Cytoplasmic1 PublicationAdd BLAST48

    Keywords - Cellular componenti

    Cell membrane, Membrane

    <p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

    <p>This subsection of the ‘Pathology and Biotech’ section provides information on the disease(s) associated with genetic variations in a given protein. The information is extracted from the scientific literature and diseases that are also described in the <a href="http://www.ncbi.nlm.nih.gov/sites/entrez?db=omim">OMIM</a> database are represented with a <a href="http://www.uniprot.org/diseases">controlled vocabulary</a> in the following way:<p><a href='/help/involvement_in_disease' target='_top'>More...</a></p>Involvement in diseasei

    Episodic ataxia 6 (EA6)1 Publication
    The disease is caused by mutations affecting the gene represented in this entry.
    Disease descriptionA disorder characterized by episodic ataxia, seizures, migraine and alternating hemiplegia.
    Related information in OMIM
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the ‘Sequence’ section describes natural variant(s) of the protein sequence.<p><a href='/help/variant' target='_top'>More...</a></p>Natural variantiVAR_031733290P → R in EA6. 1 PublicationCorresponds to variant dbSNP:rs137852619EnsemblClinVar.1

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/manual/pathology_and_biotech_section">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi363S → R: Loss of electrogenic glutamate transport. Strongly decreased L-aspartate and L-glutamate uptake combined with strongly increased permeability ot other ions; when associated with M-477. 1 Publication1
    Mutagenesisi477R → M: Strongly decreased L-aspartate and L-glutamate uptake combined with strongly increased permeability ot other ions; when associated with R-363. 1 Publication1
    Mutagenesisi523Y → F: No effect on activity. 1 Publication1

    Keywords - Diseasei

    Disease mutation

    Organism-specific databases

    DisGeNET

    More...
    DisGeNETi
    6507

    MalaCards human disease database

    More...
    MalaCardsi
    SLC1A3
    MIMi612656 phenotype

    Open Targets

    More...
    OpenTargetsi
    ENSG00000079215

    Orphanet; a database dedicated to information on rare diseases and orphan drugs

    More...
    Orphaneti
    2131 Alternating hemiplegia of childhood
    209967 Episodic ataxia type 6

    The Pharmacogenetics and Pharmacogenomics Knowledge Base

    More...
    PharmGKBi
    PA35828

    Miscellaneous databases

    Pharos NIH Druggable Genome Knowledgebase

    More...
    Pharosi
    P43003

    Chemistry databases

    ChEMBL database of bioactive drug-like small molecules

    More...
    ChEMBLi
    CHEMBL3085

    Drug and drug target database

    More...
    DrugBanki
    DB00142 Glutamic Acid

    DrugCentral

    More...
    DrugCentrali
    P43003

    IUPHAR/BPS Guide to PHARMACOLOGY

    More...
    GuidetoPHARMACOLOGYi
    868

    Polymorphism and mutation databases

    BioMuta curated single-nucleotide variation and disease association database

    More...
    BioMutai
    SLC1A3

    Domain mapping of disease mutations (DMDM)

    More...
    DMDMi
    1169458

    <p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00002020571 – 542Excitatory amino acid transporter 1Add BLAST542

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the ‘PTM / Processing’ section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei512PhosphoserineCombined sources1

    <p>This subsection of the <a href="http://www.uniprot.org/help/ptm_processing_section">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationi

    Glycosylated.By similarity

    Keywords - PTMi

    Glycoprotein, Phosphoprotein

    Proteomic databases

    jPOST - Japan Proteome Standard Repository/Database

    More...
    jPOSTi
    P43003

    MassIVE - Mass Spectrometry Interactive Virtual Environment

    More...
    MassIVEi
    P43003

    MaxQB - The MaxQuant DataBase

    More...
    MaxQBi
    P43003

    PaxDb, a database of protein abundance averages across all three domains of life

    More...
    PaxDbi
    P43003

    PeptideAtlas

    More...
    PeptideAtlasi
    P43003

    PRoteomics IDEntifications database

    More...
    PRIDEi
    P43003

    ProteomicsDB: a multi-organism proteome resource

    More...
    ProteomicsDBi
    55567 [P43003-1]
    55568 [P43003-2]

    PTM databases

    iPTMnet integrated resource for PTMs in systems biology context

    More...
    iPTMneti
    P43003

    Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

    More...
    PhosphoSitePlusi
    P43003

    SwissPalm database of S-palmitoylation events

    More...
    SwissPalmi
    P43003

    <p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

    <p>This subsection of the ‘Expression’ section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified ‘at protein level’. <br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityi

    Detected in brain (PubMed:8218410, PubMed:7521911, PubMed:8123008). Detected at very much lower levels in heart, lung, placenta and skeletal muscle (PubMed:7521911, PubMed:8123008). Highly expressed in cerebellum, but also found in frontal cortex, hippocampus and basal ganglia (PubMed:7521911).3 Publications

    Gene expression databases

    Bgee dataBase for Gene Expression Evolution

    More...
    Bgeei
    ENSG00000079215 Expressed in 210 organ(s), highest expression level in cerebellum

    ExpressionAtlas, Differential and Baseline Expression

    More...
    ExpressionAtlasi
    P43003 baseline and differential

    Genevisible search portal to normalized and curated expression data from Genevestigator

    More...
    Genevisiblei
    P43003 HS

    Organism-specific databases

    Human Protein Atlas

    More...
    HPAi
    CAB002573
    HPA037467
    HPA037468

    <p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

    <p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

    Homotrimer.

    1 Publication

    Protein-protein interaction databases

    The Biological General Repository for Interaction Datasets (BioGrid)

    More...
    BioGridi
    112398, 10 interactors

    Protein interaction database and analysis system

    More...
    IntActi
    P43003, 11 interactors

    STRING: functional protein association networks

    More...
    STRINGi
    9606.ENSP00000265113

    Chemistry databases

    BindingDB database of measured binding affinities

    More...
    BindingDBi
    P43003

    <p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

    Secondary structure

    1542
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details

    3D structure databases

    SWISS-MODEL Repository - a database of annotated 3D protein structure models

    More...
    SMRi
    P43003

    Database of comparative protein structure models

    More...
    ModBasei
    Search...

    Protein Data Bank in Europe - Knowledge Base

    More...
    PDBe-KBi
    Search...

    <p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the ‘Family and Domains’ section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni363 – 365Aspartate bindingCombined sources1 Publication3
    Regioni443 – 447Aspartate bindingCombined sources1 Publication5

    <p>This subsection of the ‘Family and domains’ section provides general information on the biological role of a domain. The term ‘domain’ is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

    Contains eight transmembrane regions plus two helical hairpins that dip into the membrane. These helical hairpin structures play an important role in the transport process. The first enters the membrane from the cytoplasmic side, the second one from the extracellular side. During the transport cycle, the regions involved in amino acid transport, and especially the helical hairpins, move vertically by about 15-18 Angstroms, alternating between exposure to the aqueous phase and reinsertion in the lipid bilayer. In contrast, the regions involved in trimerization do not move.1 Publication

    <p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    evolutionary genealogy of genes: Non-supervised Orthologous Groups

    More...
    eggNOGi
    KOG3787 Eukaryota
    COG1301 LUCA

    Ensembl GeneTree

    More...
    GeneTreei
    ENSGT00940000155464

    The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

    More...
    HOGENOMi
    HOG000208776

    InParanoid: Eukaryotic Ortholog Groups

    More...
    InParanoidi
    P43003

    KEGG Orthology (KO)

    More...
    KOi
    K05614

    Identification of Orthologs from Complete Genome Data

    More...
    OMAi
    GIMFVVH

    Database of Orthologous Groups

    More...
    OrthoDBi
    1604981at2759

    Database for complete collections of gene phylogenies

    More...
    PhylomeDBi
    P43003

    TreeFam database of animal gene trees

    More...
    TreeFami
    TF315206

    Family and domain databases

    Gene3D Structural and Functional Annotation of Protein Families

    More...
    Gene3Di
    1.10.3860.10, 2 hits

    Integrated resource of protein families, domains and functional sites

    More...
    InterProi
    View protein in InterPro
    IPR001991 Na-dicarboxylate_symporter
    IPR018107 Na-dicarboxylate_symporter_CS
    IPR036458 Na:dicarbo_symporter_sf

    Pfam protein domain database

    More...
    Pfami
    View protein in Pfam
    PF00375 SDF, 1 hit

    Superfamily database of structural and functional annotation

    More...
    SUPFAMi
    SSF118215 SSF118215, 1 hit

    PROSITE; a protein domain and family database

    More...
    PROSITEi
    View protein in PROSITE
    PS00713 NA_DICARBOXYL_SYMP_1, 1 hit
    PS00714 NA_DICARBOXYL_SYMP_2, 1 hit

    <p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (2+)i

    <p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

    This entry describes 2 <p>This subsection of the ‘Sequence’ section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by alternative splicing. AlignAdd to basket

    This entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All

    Isoform 1 (identifier: P43003-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the <div> <p><b>What is the canonical sequence?</b><p><a href='/help/canonical_and_isoforms' target='_top'>More...</a></p>canonicali sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide
            10         20         30         40         50
    MTKSNGEEPK MGGRMERFQQ GVRKRTLLAK KKVQNITKED VKSYLFRNAF
    60 70 80 90 100
    VLLTVTAVIV GTILGFTLRP YRMSYREVKY FSFPGELLMR MLQMLVLPLI
    110 120 130 140 150
    ISSLVTGMAA LDSKASGKMG MRAVVYYMTT TIIAVVIGII IVIIIHPGKG
    160 170 180 190 200
    TKENMHREGK IVRVTAADAF LDLIRNMFPP NLVEACFKQF KTNYEKRSFK
    210 220 230 240 250
    VPIQANETLV GAVINNVSEA METLTRITEE LVPVPGSVNG VNALGLVVFS
    260 270 280 290 300
    MCFGFVIGNM KEQGQALREF FDSLNEAIMR LVAVIMWYAP VGILFLIAGK
    310 320 330 340 350
    IVEMEDMGVI GGQLAMYTVT VIVGLLIHAV IVLPLLYFLV TRKNPWVFIG
    360 370 380 390 400
    GLLQALITAL GTSSSSATLP ITFKCLEENN GVDKRVTRFV LPVGATINMD
    410 420 430 440 450
    GTALYEALAA IFIAQVNNFE LNFGQIITIS ITATAASIGA AGIPQAGLVT
    460 470 480 490 500
    MVIVLTSVGL PTDDITLIIA VDWFLDRLRT TTNVLGDSLG AGIVEHLSRH
    510 520 530 540
    ELKNRDVEMG NSVIEENEMK KPYQLIAQDN ETEKPIDSET KM
    Length:542
    Mass (Da):59,572
    Last modified:November 1, 1995 - v1
    <p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i6E9F62D35A3A5A29
    GO
    Isoform 2 (identifier: P43003-2) [UniParc]FASTAAdd to basket
    Also known as: EAAT1ex9skip

    The sequence of this isoform differs from the canonical sequence as follows:
         430-475: SITATAASIGAAGIPQAGLVTMVIVLTSVGLPTDDITLIIAVDWFL → R

    Note: Expressed throughout the CNS, both in gray matter and axonal tracts, at levels ranging between 10% and 20% of isoform 1. Localizes to ER, has no functional glutamate uptake activity, and exerts a dominant negative effect isoform 1.
    Show »
    Length:497
    Mass (Da):55,149
    Checksum:i7C21EC2B467E1C11
    GO

    <p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

    There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
    EntryEntry nameProtein names
    Gene namesLengthAnnotation
    E7EUS7E7EUS7_HUMAN
    Amino acid transporter
    SLC1A3
    101Annotation score:

    Annotation score:2 out of 5

    <p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
    E7EUV6E7EUV6_HUMAN
    Amino acid transporter
    SLC1A3
    106Annotation score:

    Annotation score:2 out of 5

    <p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
    A0A087X0U3A0A087X0U3_HUMAN
    Amino acid transporter
    SLC1A3
    430Annotation score:

    Annotation score:2 out of 5

    <p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
    A0A087WT87A0A087WT87_HUMAN
    Amino acid transporter
    SLC1A3
    496Annotation score:

    Annotation score:2 out of 5

    <p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the ‘Sequence’ section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti366S → CT (PubMed:8218410).Curated1

    Natural variant

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Natural variantiVAR_011877219E → D. Corresponds to variant dbSNP:rs2032892EnsemblClinVar.1
    Natural variantiVAR_031733290P → R in EA6. 1 PublicationCorresponds to variant dbSNP:rs137852619EnsemblClinVar.1

    Alternative sequence

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the ‘Sequence’ section describes the sequence of naturally occurring alternative protein isoform(s). The changes in the amino acid sequence may be due to alternative splicing, alternative promoter usage, alternative initiation, or ribosomal frameshifting.<p><a href='/help/var_seq' target='_top'>More...</a></p>Alternative sequenceiVSP_043913430 – 475SITAT…VDWFL → R in isoform 2. 1 PublicationAdd BLAST46

    Sequence databases

    Select the link destinations:

    EMBL nucleotide sequence database

    More...
    EMBLi

    GenBank nucleotide sequence database

    More...
    GenBanki

    DNA Data Bank of Japan; a nucleotide sequence database

    More...
    DDBJi
    Links Updated
    L19158 mRNA No translation available.
    U03504 mRNA Translation: AAA50428.1
    D26443 mRNA Translation: BAA05462.1
    Z31713
    , Z31703, Z31704, Z31705, Z31706, Z31707, Z31708, Z31709, Z31710 Genomic DNA Translation: CAA83507.1
    AY954110 mRNA Translation: AAY28724.1
    AK312304 mRNA Translation: BAG35230.1
    AC008957 Genomic DNA No translation available.
    AC010631 Genomic DNA No translation available.
    CH471119 Genomic DNA Translation: EAW55945.1

    The Consensus CDS (CCDS) project

    More...
    CCDSi
    CCDS3919.1 [P43003-1]
    CCDS54844.1 [P43003-2]

    Protein sequence database of the Protein Information Resource

    More...
    PIRi
    S38353

    NCBI Reference Sequences

    More...
    RefSeqi
    NP_001160167.1, NM_001166695.2 [P43003-2]
    NP_001276869.1, NM_001289940.1
    NP_004163.3, NM_004172.4 [P43003-1]
    XP_005248399.1, XM_005248342.2 [P43003-1]

    Genome annotation databases

    Ensembl eukaryotic genome annotation project

    More...
    Ensembli
    ENST00000265113; ENSP00000265113; ENSG00000079215 [P43003-1]
    ENST00000381918; ENSP00000371343; ENSG00000079215 [P43003-2]

    Database of genes from NCBI RefSeq genomes

    More...
    GeneIDi
    6507

    KEGG: Kyoto Encyclopedia of Genes and Genomes

    More...
    KEGGi
    hsa:6507

    UCSC genome browser

    More...
    UCSCi
    uc003jkj.4 human [P43003-1]

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    <p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

    <p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    L19158 mRNA No translation available.
    U03504 mRNA Translation: AAA50428.1
    D26443 mRNA Translation: BAA05462.1
    Z31713
    , Z31703, Z31704, Z31705, Z31706, Z31707, Z31708, Z31709, Z31710 Genomic DNA Translation: CAA83507.1
    AY954110 mRNA Translation: AAY28724.1
    AK312304 mRNA Translation: BAG35230.1
    AC008957 Genomic DNA No translation available.
    AC010631 Genomic DNA No translation available.
    CH471119 Genomic DNA Translation: EAW55945.1
    CCDSiCCDS3919.1 [P43003-1]
    CCDS54844.1 [P43003-2]
    PIRiS38353
    RefSeqiNP_001160167.1, NM_001166695.2 [P43003-2]
    NP_001276869.1, NM_001289940.1
    NP_004163.3, NM_004172.4 [P43003-1]
    XP_005248399.1, XM_005248342.2 [P43003-1]

    3D structure databases

    Select the link destinations:

    Protein Data Bank Europe

    More...
    PDBei

    Protein Data Bank RCSB

    More...
    RCSB PDBi

    Protein Data Bank Japan

    More...
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    5LLMX-ray3.25A1-148[»]
    A243-542[»]
    5LLUX-ray3.32A1-148[»]
    A243-542[»]
    5LM4X-ray3.10A1-148[»]
    A243-542[»]
    5MJUX-ray3.71A1-148[»]
    A243-542[»]
    SMRiP43003
    ModBaseiSearch...
    PDBe-KBiSearch...

    Protein-protein interaction databases

    BioGridi112398, 10 interactors
    IntActiP43003, 11 interactors
    STRINGi9606.ENSP00000265113

    Chemistry databases

    BindingDBiP43003
    ChEMBLiCHEMBL3085
    DrugBankiDB00142 Glutamic Acid
    DrugCentraliP43003
    GuidetoPHARMACOLOGYi868

    Protein family/group databases

    TCDBi2.A.23.2.6 the dicarboxylate/amino acid:cation (na(+) or h(+)) symporter (daacs) family

    PTM databases

    iPTMnetiP43003
    PhosphoSitePlusiP43003
    SwissPalmiP43003

    Polymorphism and mutation databases

    BioMutaiSLC1A3
    DMDMi1169458

    Proteomic databases

    jPOSTiP43003
    MassIVEiP43003
    MaxQBiP43003
    PaxDbiP43003
    PeptideAtlasiP43003
    PRIDEiP43003
    ProteomicsDBi55567 [P43003-1]
    55568 [P43003-2]

    Genome annotation databases

    EnsembliENST00000265113; ENSP00000265113; ENSG00000079215 [P43003-1]
    ENST00000381918; ENSP00000371343; ENSG00000079215 [P43003-2]
    GeneIDi6507
    KEGGihsa:6507
    UCSCiuc003jkj.4 human [P43003-1]

    Organism-specific databases

    Comparative Toxicogenomics Database

    More...
    CTDi
    6507
    DisGeNETi6507

    GeneCards: human genes, protein and diseases

    More...
    GeneCardsi
    SLC1A3
    HGNCiHGNC:10941 SLC1A3
    HPAiCAB002573
    HPA037467
    HPA037468
    MalaCardsiSLC1A3
    MIMi600111 gene
    612656 phenotype
    neXtProtiNX_P43003
    OpenTargetsiENSG00000079215
    Orphaneti2131 Alternating hemiplegia of childhood
    209967 Episodic ataxia type 6
    PharmGKBiPA35828

    GenAtlas: human gene database

    More...
    GenAtlasi
    Search...

    Phylogenomic databases

    eggNOGiKOG3787 Eukaryota
    COG1301 LUCA
    GeneTreeiENSGT00940000155464
    HOGENOMiHOG000208776
    InParanoidiP43003
    KOiK05614
    OMAiGIMFVVH
    OrthoDBi1604981at2759
    PhylomeDBiP43003
    TreeFamiTF315206

    Enzyme and pathway databases

    ReactomeiR-HSA-210455 Astrocytic Glutamate-Glutamine Uptake And Metabolism
    R-HSA-210500 Glutamate Neurotransmitter Release Cycle
    R-HSA-425393 Transport of inorganic cations/anions and amino acids/oligopeptides
    R-HSA-5619062 Defective SLC1A3 causes episodic ataxia 6 (EA6)

    Miscellaneous databases

    ChiTaRS: a database of human, mouse and fruit fly chimeric transcripts and RNA-sequencing data

    More...
    ChiTaRSi
    SLC1A3 human

    The Gene Wiki collection of pages on human genes and proteins

    More...
    GeneWikii
    Glutamate_aspartate_transporter

    Database of phenotypes from RNA interference screens in Drosophila and Homo sapiens

    More...
    GenomeRNAii
    6507
    PharosiP43003

    Protein Ontology

    More...
    PROi
    PR:P43003

    The Stanford Online Universal Resource for Clones and ESTs

    More...
    SOURCEi
    Search...

    Gene expression databases

    BgeeiENSG00000079215 Expressed in 210 organ(s), highest expression level in cerebellum
    ExpressionAtlasiP43003 baseline and differential
    GenevisibleiP43003 HS

    Family and domain databases

    Gene3Di1.10.3860.10, 2 hits
    InterProiView protein in InterPro
    IPR001991 Na-dicarboxylate_symporter
    IPR018107 Na-dicarboxylate_symporter_CS
    IPR036458 Na:dicarbo_symporter_sf
    PfamiView protein in Pfam
    PF00375 SDF, 1 hit
    SUPFAMiSSF118215 SSF118215, 1 hit
    PROSITEiView protein in PROSITE
    PS00713 NA_DICARBOXYL_SYMP_1, 1 hit
    PS00714 NA_DICARBOXYL_SYMP_2, 1 hit

    ProtoNet; Automatic hierarchical classification of proteins

    More...
    ProtoNeti
    Search...

    MobiDB: a database of protein disorder and mobility annotations

    More...
    MobiDBi
    Search...

    <p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

    <p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiEAA1_HUMAN
    <p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P43003
    Secondary accession number(s): B2R5T3, Q4JCQ8
    <p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: November 13, 2019
    This is version 184 of the entry and version 1 of the sequence. See complete history.
    <p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    <p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    2. SIMILARITY comments
      Index of protein domains and families
    3. Human chromosome 5
      Human chromosome 5: entries, gene names and cross-references to MIM
    4. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    5. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    6. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health

    We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

    Do not show this banner again