UniProtKB - P42907 (AGAS_ECOLI)
Protein
Putative D-galactosamine-6-phosphate deaminase AgaS
Gene
agaS
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the isomerization-deamination of galactosamine 6-phosphate to form tagatofuranose 6-phosphate and ammonium ion.By similarity
Miscellaneous
In contrast to E.coli strains C and EC3132, K-12 strains cannot grow on N-acetylgalactosamine and D-galactosamine, because they carry a deletion and thus lack active PTS systems specific for these compounds. Therefore, AgaS in K-12 strains is not involved in the degradation of these compounds.
Catalytic activityi
GO - Molecular functioni
- carbohydrate derivative binding Source: InterPro
- hydrolase activity Source: UniProtKB-KW
- isomerase activity Source: InterPro
GO - Biological processi
- carbohydrate derivative metabolic process Source: InterPro
- phosphoenolpyruvate-dependent sugar phosphotransferase system Source: GO_Central
Keywordsi
Molecular function | Hydrolase |
Enzyme and pathway databases
BioCyci | EcoCyc:G7634-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Putative D-galactosamine-6-phosphate deaminase AgaSCurated (EC:3.5.99.-By similarity)Alternative name(s): Gam-6-P deaminase/isomeraseBy similarity |
Gene namesi | Name:agaS Synonyms:yraB Ordered Locus Names:b3136, JW3105 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000136590 | 1 – 384 | Putative D-galactosamine-6-phosphate deaminase AgaSAdd BLAST | 384 |
Proteomic databases
PaxDbi | P42907 |
PRIDEi | P42907 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4261158, 779 interactors |
IntActi | P42907, 5 interactors |
STRINGi | 511145.b3136 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P42907 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P42907 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 45 – 197 | SIS 1PROSITE-ProRule annotationAdd BLAST | 153 | |
Domaini | 215 – 364 | SIS 2PROSITE-ProRule annotationAdd BLAST | 150 |
Sequence similaritiesi
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | COG2222, Bacteria |
HOGENOMi | CLU_012520_0_0_6 |
InParanoidi | P42907 |
PhylomeDBi | P42907 |
Family and domain databases
CDDi | cd05010, SIS_AgaS_like, 1 hit cd05008, SIS_GlmS_GlmD_1, 1 hit |
InterProi | View protein in InterPro IPR035466, GlmS/AgaS_SIS IPR001347, SIS IPR035464, SIS_AgaS IPR014180, Sugar_isomerase_AgaS |
Pfami | View protein in Pfam PF01380, SIS, 2 hits |
TIGRFAMsi | TIGR02815, agaS_fam, 1 hit |
PROSITEi | View protein in PROSITE PS51464, SIS, 2 hits |
i Sequence
Sequence statusi: Complete.
P42907-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPENYTPAAA ATGTWTEEEI RHQPRAWIRS LTNIDALRSA LNNFLEPLLR
60 70 80 90 100
KENLRIILTG AGTSAFIGDI IAPWLASHTG KNFSAVPTTD LVTNPMDYLN
110 120 130 140 150
PAHPLLLISF GRSGNSPESV AAVELANQFV PECYHLPITC NEAGALYQNA
160 170 180 190 200
INSDNAFALL MPAETHDRGF AMTSSITTMM ASCLAVFAPE TINSQTFRDV
210 220 230 240 250
ADRCQAILTS LGDFSEGVFG YAPWKRIVYL GSGGLQGAAR ESALKVLELT
260 270 280 290 300
AGKLAAFYDS PTGFRHGPKS LVDDETLVVV FVSSHPYTRQ YDLDLLAELR
310 320 330 340 350
RDNQAMRVIA IAAESSDIVA AGPHIILPPS RHFIDVEQAF CFLMYAQTFA
360 370 380
LMQSLHMGNT PDTPSASGTV NRVVQGVIIH PWQA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U18997 Genomic DNA Translation: AAA57939.1 U00096 Genomic DNA Translation: AAC76170.1 AP009048 Genomic DNA Translation: BAE77182.1 |
PIRi | D65103 |
RefSeqi | NP_417605.1, NC_000913.3 WP_001114869.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC76170; AAC76170; b3136 BAE77182; BAE77182; BAE77182 |
GeneIDi | 947645 |
KEGGi | ecj:JW3105 eco:b3136 |
PATRICi | fig|1411691.4.peg.3594 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U18997 Genomic DNA Translation: AAA57939.1 U00096 Genomic DNA Translation: AAC76170.1 AP009048 Genomic DNA Translation: BAE77182.1 |
PIRi | D65103 |
RefSeqi | NP_417605.1, NC_000913.3 WP_001114869.1, NZ_LN832404.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3C3J | X-ray | 1.80 | A/B/C/D/E/F | 1-384 | [»] | |
SMRi | P42907 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4261158, 779 interactors |
IntActi | P42907, 5 interactors |
STRINGi | 511145.b3136 |
Proteomic databases
PaxDbi | P42907 |
PRIDEi | P42907 |
Protocols and materials databases
DNASUi | 947645 |
Genome annotation databases
EnsemblBacteriai | AAC76170; AAC76170; b3136 BAE77182; BAE77182; BAE77182 |
GeneIDi | 947645 |
KEGGi | ecj:JW3105 eco:b3136 |
PATRICi | fig|1411691.4.peg.3594 |
Organism-specific databases
EchoBASEi | EB2620 |
Phylogenomic databases
eggNOGi | COG2222, Bacteria |
HOGENOMi | CLU_012520_0_0_6 |
InParanoidi | P42907 |
PhylomeDBi | P42907 |
Enzyme and pathway databases
BioCyci | EcoCyc:G7634-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P42907 |
PROi | PR:P42907 |
Family and domain databases
CDDi | cd05010, SIS_AgaS_like, 1 hit cd05008, SIS_GlmS_GlmD_1, 1 hit |
InterProi | View protein in InterPro IPR035466, GlmS/AgaS_SIS IPR001347, SIS IPR035464, SIS_AgaS IPR014180, Sugar_isomerase_AgaS |
Pfami | View protein in Pfam PF01380, SIS, 2 hits |
TIGRFAMsi | TIGR02815, agaS_fam, 1 hit |
PROSITEi | View protein in PROSITE PS51464, SIS, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AGAS_ECOLI | |
Accessioni | P42907Primary (citable) accession number: P42907 Secondary accession number(s): Q2M974 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | November 1, 1995 | |
Last modified: | April 7, 2021 | |
This is version 131 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families