UniProtKB - P42891 (ECE1_BOVIN)
Protein
Endothelin-converting enzyme 1
Gene
ECE1
Organism
Bos taurus (Bovine)
Status
Functioni
Converts big endothelin-1 to endothelin-1.
Catalytic activityi
- Hydrolysis of the 21-Trp-|-Val-22 bond in big endothelin to form endothelin 1. EC:3.4.24.71
Cofactori
Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity
Activity regulationi
Inhibited by phosphoramidon.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 591 | Zinc; catalyticPROSITE-ProRule annotation | 1 | |
Active sitei | 592 | PROSITE-ProRule annotation | 1 | |
Metal bindingi | 595 | Zinc; catalyticPROSITE-ProRule annotation | 1 | |
Metal bindingi | 651 | Zinc; catalyticPROSITE-ProRule annotation | 1 | |
Active sitei | 655 | Proton donorPROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- endopeptidase activity Source: BHF-UCL
- metal ion binding Source: UniProtKB-KW
- metalloendopeptidase activity Source: BHF-UCL
- protein homodimerization activity Source: BHF-UCL
GO - Biological processi
- peptide hormone processing Source: BHF-UCL
- protein processing Source: BHF-UCL
- proteolysis Source: AgBase
Keywordsi
Molecular function | Hydrolase, Metalloprotease, Protease |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-BTA-375276 Peptide ligand-binding receptors |
SABIO-RKi | P42891 |
Protein family/group databases
MEROPSi | M13.002 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:ECE1 |
Organismi | Bos taurus (Bovine) |
Taxonomic identifieri | 9913 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Proteomesi |
|
Organism-specific databases
VGNCi | VGNC:28302 ECE1 |
Subcellular locationi
Plasma membrane
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
- membrane Source: BHF-UCL
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 52 | CytoplasmicSequence analysisAdd BLAST | 52 | |
Transmembranei | 53 – 73 | Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST | 21 | |
Topological domaini | 74 – 754 | ExtracellularSequence analysisAdd BLAST | 681 |
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000078218 | 1 – 754 | Endothelin-converting enzyme 1Add BLAST | 754 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 9 | PhosphothreonineBy similarity | 1 | |
Disulfide bondi | 83 ↔ 88 | PROSITE-ProRule annotation | ||
Disulfide bondi | 106 ↔ 739 | PROSITE-ProRule annotation | ||
Disulfide bondi | 114 ↔ 699 | PROSITE-ProRule annotation | ||
Glycosylationi | 150 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 169 ↔ 419 | PROSITE-ProRule annotation | ||
Glycosylationi | 171 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 194 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 254 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 300 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 346 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 367 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 523 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 616 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 628 ↔ 751 | PROSITE-ProRule annotation | ||
Glycosylationi | 635 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
Disulfide bond, Glycoprotein, PhosphoproteinProteomic databases
PaxDbi | P42891 |
PeptideAtlasi | P42891 |
PRIDEi | P42891 |
Expressioni
Gene expression databases
Bgeei | ENSBTAG00000002977 Expressed in 9 organ(s), highest expression level in lung |
ExpressionAtlasi | P42891 baseline and differential |
Interactioni
Subunit structurei
Homodimer; disulfide-linked (By similarity).
Interacts with PPP1R16B (PubMed:26806547).
By similarity1 PublicationGO - Molecular functioni
- protein homodimerization activity Source: BHF-UCL
Protein-protein interaction databases
STRINGi | 9913.ENSBTAP00000055669 |
Chemistry databases
BindingDBi | P42891 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 82 – 754 | Peptidase M13PROSITE-ProRule annotationAdd BLAST | 673 |
Sequence similaritiesi
Keywords - Domaini
Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3624 Eukaryota COG3590 LUCA |
GeneTreei | ENSGT00940000156050 |
InParanoidi | P42891 |
OrthoDBi | 282463at2759 |
Family and domain databases
CDDi | cd08662 M13, 1 hit |
Gene3Di | 1.10.1380.10, 1 hit 3.40.390.10, 1 hit |
InterProi | View protein in InterPro IPR029734 ECE1 IPR024079 MetalloPept_cat_dom_sf IPR000718 Peptidase_M13 IPR018497 Peptidase_M13_C IPR042089 Peptidase_M13_dom_2 IPR008753 Peptidase_M13_N |
PANTHERi | PTHR11733 PTHR11733, 1 hit PTHR11733:SF130 PTHR11733:SF130, 1 hit |
Pfami | View protein in Pfam PF01431 Peptidase_M13, 1 hit PF05649 Peptidase_M13_N, 1 hit |
PRINTSi | PR00786 NEPRILYSIN |
PROSITEi | View protein in PROSITE PS51885 NEPRILYSIN, 1 hit PS00142 ZINC_PROTEASE, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 6 potential isoforms that are computationally mapped.Show allAlign All
P42891-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MMSTYKRATL DEEDLVDSLS ESDVYPNHLQ VNFRGPRNGQ RCWAARTPVE
60 70 80 90 100
KRLVVLVALL AAALVACLAV LGIQYQTRTP SVCLSEACIS VTSSILSSMD
110 120 130 140 150
PTVDPCQDFF TYACGGWIKA NPVPDGHSRW GTFSNLWEHN QAIIKHLLEN
160 170 180 190 200
STASVSEAER KAQVYYRACM NETRIEELKA KPLMELIEKL GGWNITGPWD
210 220 230 240 250
KDNFQDTLQV VTSHYHTSPF FSVYVSADSK NSNSNVIQVD QSGLGLPSRD
260 270 280 290 300
YYLNKTENEK VLTGYLNYMV QLGKLLGGGA EDTIRPQMQQ ILDFETALAN
310 320 330 340 350
ITIPQEKRRD EELIYHKVTA AELQTLAPAI NWLPFLNTIF YPVEINESEP
360 370 380 390 400
IVIYDKEYLS KVSTLINSTD KCLLNNYMIW NLVRKTSSFL DQRFQDADEK
410 420 430 440 450
FMEVMYGTKK TCLPRWKFCV SDTENTLGFA LGPMFVKATF AEDSKNIASE
460 470 480 490 500
IILEIKKAFE ESLSTLKWMD EDTRKSAKEK ADAIYNMIGY PNFIMDPKEL
510 520 530 540 550
DKVFNDYTAV PDLYFENAMR FFNFSWRVTA DQLRKAPNRD QWSMTPPMVN
560 570 580 590 600
AYYSPTKNEI VFPAGILQAP FYTRSSPNAL NFGGIGVVVG HELTHAFDDQ
610 620 630 640 650
GREYDKDGNL RPWWKNSSVE AFKQQTACMV EQYGNYSVNG EPVNGRHTLG
660 670 680 690 700
ENIADNGGLK AAYRAYQNWV KKNGAEQTLP TLGLTNNQLF FLSFAQVWCS
710 720 730 740 750
VRTPESSHEG LITDPHSPSR FRVIGSISNS KEFSEHFHCP PGSPMNPHHK
CEVW
Computationally mapped potential isoform sequencesi
There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A3Q1NG29 | A0A3Q1NG29_BOVIN | Endothelin-converting enzyme 1 | ECE1 | 771 | Annotation score: | ||
A0A3Q1M0N7 | A0A3Q1M0N7_BOVIN | Endothelin-converting enzyme 1 | ECE1 | 753 | Annotation score: | ||
G3N1D0 | G3N1D0_BOVIN | Endothelin-converting enzyme 1 | ECE1 | 752 | Annotation score: | ||
A0A3Q1MDU6 | A0A3Q1MDU6_BOVIN | Endothelin-converting enzyme 1 | ECE1 | 751 | Annotation score: | ||
A0A3Q1MWP2 | A0A3Q1MWP2_BOVIN | Endothelin-converting enzyme 1 | ECE1 | 767 | Annotation score: | ||
F1N6J1 | F1N6J1_BOVIN | Endothelin-converting enzyme 1 | ECE1 | 339 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 8 | A → P in CAA84547 (PubMed:7805846).Curated | 1 | |
Sequence conflicti | 120 | A → S in AAI33321 (Ref. 2) Curated | 1 | |
Sequence conflicti | 162 – 164 | AQV → DQE in CAA84547 (PubMed:7805846).Curated | 3 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z35306 mRNA Translation: CAA84547.1 BC133320 mRNA Translation: AAI33321.1 |
PIRi | S51010 S47268 |
RefSeqi | XP_005203129.1, XM_005203072.3 |
Genome annotation databases
Ensembli | ENSBTAT00000037770; ENSBTAP00000037593; ENSBTAG00000002977 ENSBTAT00000076810; ENSBTAP00000071468; ENSBTAG00000002977 |
GeneIDi | 281133 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z35306 mRNA Translation: CAA84547.1 BC133320 mRNA Translation: AAI33321.1 |
PIRi | S51010 S47268 |
RefSeqi | XP_005203129.1, XM_005203072.3 |
3D structure databases
SMRi | P42891 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9913.ENSBTAP00000055669 |
Chemistry databases
BindingDBi | P42891 |
ChEMBLi | CHEMBL2340 |
Protein family/group databases
MEROPSi | M13.002 |
Proteomic databases
PaxDbi | P42891 |
PeptideAtlasi | P42891 |
PRIDEi | P42891 |
Genome annotation databases
Ensembli | ENSBTAT00000037770; ENSBTAP00000037593; ENSBTAG00000002977 ENSBTAT00000076810; ENSBTAP00000071468; ENSBTAG00000002977 |
GeneIDi | 281133 |
Organism-specific databases
CTDi | 1889 |
VGNCi | VGNC:28302 ECE1 |
Phylogenomic databases
eggNOGi | KOG3624 Eukaryota COG3590 LUCA |
GeneTreei | ENSGT00940000156050 |
InParanoidi | P42891 |
OrthoDBi | 282463at2759 |
Enzyme and pathway databases
Reactomei | R-BTA-375276 Peptide ligand-binding receptors |
SABIO-RKi | P42891 |
Miscellaneous databases
PROi | PR:P42891 |
Gene expression databases
Bgeei | ENSBTAG00000002977 Expressed in 9 organ(s), highest expression level in lung |
ExpressionAtlasi | P42891 baseline and differential |
Family and domain databases
CDDi | cd08662 M13, 1 hit |
Gene3Di | 1.10.1380.10, 1 hit 3.40.390.10, 1 hit |
InterProi | View protein in InterPro IPR029734 ECE1 IPR024079 MetalloPept_cat_dom_sf IPR000718 Peptidase_M13 IPR018497 Peptidase_M13_C IPR042089 Peptidase_M13_dom_2 IPR008753 Peptidase_M13_N |
PANTHERi | PTHR11733 PTHR11733, 1 hit PTHR11733:SF130 PTHR11733:SF130, 1 hit |
Pfami | View protein in Pfam PF01431 Peptidase_M13, 1 hit PF05649 Peptidase_M13_N, 1 hit |
PRINTSi | PR00786 NEPRILYSIN |
PROSITEi | View protein in PROSITE PS51885 NEPRILYSIN, 1 hit PS00142 ZINC_PROTEASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ECE1_BOVIN | |
Accessioni | P42891Primary (citable) accession number: P42891 Secondary accession number(s): A2VDM9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | February 26, 2008 | |
Last modified: | October 16, 2019 | |
This is version 140 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Direct protein sequencing, Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - SIMILARITY comments
Index of protein domains and families