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Protein

UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase

Gene

Dpagt1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the initial step of dolichol-linked oligosaccharide biosynthesis in N-linked protein glycosylation pathway: transfers GlcNAc-1-P from UDP-GlcNAc onto the carrier lipid dolichyl phosphate (P-dolichol), yielding GlcNAc-P-P-dolichol.By similarity

Catalytic activityi

UDP-N-acetyl-D-glucosamine + dolichyl phosphate = UMP + N-acetyl-D-glucosaminyl-diphosphodolichol.By similarity

Cofactori

Mg2+By similarity

Activity regulationi

Activated by mannosylphosphoryldolichol and phospholipids such as phosphatidylglycerol and phosphatidylcholine. Inhibited by natural nucleoside antibiotic tunicamycin, which acts as a structural analog and competitor of UDP-GlcNAc.By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei48Inhibitor; via amide nitrogenBy similarity1
Binding sitei121InhibitorBy similarity1
Binding sitei187InhibitorBy similarity1
Binding sitei254InhibitorBy similarity1
Binding sitei305InhibitorBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase
LigandMagnesium

Enzyme and pathway databases

ReactomeiR-MMU-446193 Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein
UniPathwayi
UPA00378

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase (EC:2.7.8.15By similarity)
Alternative name(s):
GlcNAc-1-P transferase
Short name:
G1PT
Short name:
GPT
N-acetylglucosamine-1-phosphate transferase
Gene namesi
Name:Dpagt1Imported
Synonyms:Dpagt2, Gnpta
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1196396 Dpagt1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 10LumenalCurated10
Transmembranei11 – 40Helical; Name=Helix 1By similarityAdd BLAST30
Topological domaini41 – 60CytoplasmicCuratedAdd BLAST20
Transmembranei61 – 80Helical; Name=Helix 2By similarityAdd BLAST20
Topological domaini81 – 93LumenalCuratedAdd BLAST13
Transmembranei94 – 120Helical; Name=Helix 3By similarityAdd BLAST27
Topological domaini121 – 123CytoplasmicCurated3
Transmembranei124 – 145Helical; Name=Helix 4By similarityAdd BLAST22
Topological domaini146 – 168LumenalCuratedAdd BLAST23
Transmembranei169 – 188Helical; Name=Helix 5By similarityAdd BLAST20
Topological domaini189 – 194CytoplasmicCurated6
Transmembranei195 – 215Helical; Name=Helix 6By similarityAdd BLAST21
Topological domaini216 – 220LumenalCurated5
Transmembranei221 – 244Helical; Name=Helix 7By similarityAdd BLAST24
Topological domaini245 – 252CytoplasmicCurated8
Transmembranei253 – 271Helical; Name=Helix 8By similarityAdd BLAST19
Topological domaini272 – 273LumenalCurated2
Transmembranei274 – 295Helical; Name=Helix 9By similarityAdd BLAST22
Topological domaini296 – 377CytoplasmicCuratedAdd BLAST82
Transmembranei378 – 402Helical; Name=Helix 10By similarityAdd BLAST25
Topological domaini403 – 410LumenalCurated8

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice die 4 to 5 days post-fertilization, just after implantation, suggesting that protein function and N-glycosylation are essential in early embryogenesis.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001087621 – 410UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferaseAdd BLAST410

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi148N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP42867
PaxDbiP42867
PeptideAtlasiP42867
PRIDEiP42867

PTM databases

iPTMnetiP42867
PhosphoSitePlusiP42867

Expressioni

Developmental stagei

Highest activity is during the mid-phase of lactation.

Gene expression databases

BgeeiENSMUSG00000032123 Expressed in 237 organ(s), highest expression level in saliva-secreting gland
ExpressionAtlasiP42867 baseline and differential
GenevisibleiP42867 MM

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

IntActiP42867, 1 interactor
STRINGi10090.ENSMUSP00000056282

Structurei

3D structure databases

ProteinModelPortaliP42867
SMRiP42867
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi69 – 81Dolichol recognitionAdd BLAST13
Motifi224 – 236Dolichol recognitionAdd BLAST13

Sequence similaritiesi

Belongs to the glycosyltransferase 4 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2788 Eukaryota
COG0472 LUCA
GeneTreeiENSGT00390000011424
HOGENOMiHOG000163915
HOVERGENiHBG000846
InParanoidiP42867
KOiK01001
OMAiGDYRDDH
OrthoDBiEOG091G0A0M
PhylomeDBiP42867
TreeFamiTF313734

Family and domain databases

CDDicd06855 GT_GPT_euk, 1 hit
InterProiView protein in InterPro
IPR000715 Glycosyl_transferase_4
IPR033895 GPT
PANTHERiPTHR10571 PTHR10571, 1 hit
PfamiView protein in Pfam
PF00953 Glycos_transf_4, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

P42867-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MWAFPELPLP LPLLVNLIGS LLGFVATVTL IPAFRSHFIA ARLCGQDLNK
60 70 80 90 100
LSQQQIPESQ GVISGAVFLI ILFCFIPFPF LNCFVEEQCK AFPHHEFVAL
110 120 130 140 150
IGALLAICCM IFLGFADDVL NLRWRHKLLL PTAASLPLLM VYFTNFGNTT
160 170 180 190 200
IVVPKPFRWI LGLHLDLGIL YYVYMGLLAV FCTNAINILA GINGLEAGQS
210 220 230 240 250
LVISASIIVF NLVELEGDYR DDHIFSLYFM IPFFFTTLGL LYHNWYPSRV
260 270 280 290 300
FVGDTFCYFA GMTFAVVGIL GHFSKTMLLF FMPQVFNFLY SLPQLFHIIP
310 320 330 340 350
CPRHRMPRLN AKTGKLEMSY SKFKTKNLSF LGTFILKVAE NLRLVTVHQG
360 370 380 390 400
ESEDGAFTEC NNMTLINLLL KVFGPIHERN LTLLLLLLQV LSSAATFSIR
410
YQLVRLFYDV
Length:410
Mass (Da):46,412
Last modified:November 28, 2003 - v2
Checksum:iF729D6983FA67E0B
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A1L1ST50A0A1L1ST50_MOUSE
Dolichyl-phosphate (UDP-N-acetylglu...
Dpagt1 mCG_10705
59Annotation score:
A0A1L1STS5A0A1L1STS5_MOUSE
UDP-N-acetylglucosamine--dolichyl-p...
Dpagt1
106Annotation score:

RNA editingi

Edited at position 74.1 Publication
Partially edited.

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti74C → Y in RNA edited version. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X65603 mRNA Translation: CAA46553.1
BC010474 mRNA Translation: AAH10474.1
CCDSiCCDS23104.1
PIRiS24326
RefSeqiNP_031901.2, NM_007875.2
UniGeneiMm.18353

Genome annotation databases

EnsembliENSMUST00000054708; ENSMUSP00000056282; ENSMUSG00000032123
GeneIDi13478
KEGGimmu:13478
UCSCiuc009pcv.1 mouse

Keywords - Coding sequence diversityi

RNA editing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X65603 mRNA Translation: CAA46553.1
BC010474 mRNA Translation: AAH10474.1
CCDSiCCDS23104.1
PIRiS24326
RefSeqiNP_031901.2, NM_007875.2
UniGeneiMm.18353

3D structure databases

ProteinModelPortaliP42867
SMRiP42867
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP42867, 1 interactor
STRINGi10090.ENSMUSP00000056282

PTM databases

iPTMnetiP42867
PhosphoSitePlusiP42867

Proteomic databases

MaxQBiP42867
PaxDbiP42867
PeptideAtlasiP42867
PRIDEiP42867

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000054708; ENSMUSP00000056282; ENSMUSG00000032123
GeneIDi13478
KEGGimmu:13478
UCSCiuc009pcv.1 mouse

Organism-specific databases

CTDi1798
MGIiMGI:1196396 Dpagt1

Phylogenomic databases

eggNOGiKOG2788 Eukaryota
COG0472 LUCA
GeneTreeiENSGT00390000011424
HOGENOMiHOG000163915
HOVERGENiHBG000846
InParanoidiP42867
KOiK01001
OMAiGDYRDDH
OrthoDBiEOG091G0A0M
PhylomeDBiP42867
TreeFamiTF313734

Enzyme and pathway databases

UniPathwayi
UPA00378

ReactomeiR-MMU-446193 Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein

Miscellaneous databases

ChiTaRSiDpagt1 mouse
PROiPR:P42867
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000032123 Expressed in 237 organ(s), highest expression level in saliva-secreting gland
ExpressionAtlasiP42867 baseline and differential
GenevisibleiP42867 MM

Family and domain databases

CDDicd06855 GT_GPT_euk, 1 hit
InterProiView protein in InterPro
IPR000715 Glycosyl_transferase_4
IPR033895 GPT
PANTHERiPTHR10571 PTHR10571, 1 hit
PfamiView protein in Pfam
PF00953 Glycos_transf_4, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiGPT_MOUSE
AccessioniPrimary (citable) accession number: P42867
Secondary accession number(s): Q921W5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 28, 2003
Last modified: September 12, 2018
This is version 143 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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