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Protein

Polyphosphoinositide phosphatase

Gene

FIG4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Major enzyme required for hyperosmotic shock-induced turnover of PtdIns(3,5)P2 and requires VAC14 for this function. In vivo, mediates turnover of PtdIns(3,5)P2 at the vacuole membrane necessary for vacuolar size control. In vitro, catalyzes the removal of phosphate from the fifth hydroxyl of the myo-inositol ring of phosphatidylinositol 3,5-bisphosphate.4 Publications

Miscellaneous

Present with 339 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

1-phosphatidyl-1D-myo-inositol 3,5-bisphosphate + H2O = 1-phosphatidyl-1D-myo-inositol 3-phosphate + phosphate.1 Publication

Cofactori

Mg2+1 Publication

GO - Molecular functioni

  • phosphatidylinositol-3,5-bisphosphate 5-phosphatase activity Source: SGD

GO - Biological processi

Keywordsi

Molecular functionHydrolase

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER3O-86
YEAST:MONOMER3O-86
ReactomeiR-SCE-1660514 Synthesis of PIPs at the Golgi membrane
R-SCE-1660516 Synthesis of PIPs at the early endosome membrane
R-SCE-1660517 Synthesis of PIPs at the late endosome membrane

Names & Taxonomyi

Protein namesi
Recommended name:
Polyphosphoinositide phosphatase (EC:3.1.3.-)
Alternative name(s):
Factor-induced gene 4 protein
Phosphatidylinositol 3,5-bisphosphate 5-phosphatase
Gene namesi
Name:FIG4
Ordered Locus Names:YNL325C
ORF Names:N0330
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

SGDiS000005269 FIG4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane, Vacuole

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi59I → T: Defective activation of FAB1. 1 Publication1
Mutagenesisi469D → N: Partially defective in both hyperosmotic shock-induced PtdIns(3,5)P2 elevation and turnover. 1 Publication1
Mutagenesisi519G → R in FIG4-1; loss of catalytic activity in vitro. Almost 3-fold increase in PtdIns(3,5)P2 level in vivo. Partially defective in both hyperosmotic shock-induced PtdIns(3,5)P2 elevation and turnover. 3 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002097421 – 879Polyphosphoinositide phosphataseAdd BLAST879

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei829PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP42837
PaxDbiP42837
PRIDEiP42837

PTM databases

iPTMnetiP42837

Interactioni

Subunit structurei

Component of the PI(3,5)P2 regulatory complex, composed of ATG18, FIG4, FAB1, VAC14 and VAC7. VAC14 nucleates the assembly of the complex and serves as a scaffold.2 Publications

Binary interactionsi

Protein-protein interaction databases

BioGridi35516, 123 interactors
ComplexPortaliCPX-3088 PAS complex
IntActiP42837, 3 interactors
MINTiP42837
STRINGi4932.YNL325C

Structurei

3D structure databases

ProteinModelPortaliP42837
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini166 – 528SACPROSITE-ProRule annotationAdd BLAST363

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni761 – 879Required for vacuolar localizationAdd BLAST119

Phylogenomic databases

GeneTreeiENSGT00550000074943
HOGENOMiHOG000168063
InParanoidiP42837
KOiK22913
OMAiKRKCCAH
OrthoDBiEOG092C1610

Family and domain databases

InterProiView protein in InterPro
IPR002013 SAC_dom
PfamiView protein in Pfam
PF02383 Syja_N, 1 hit
PROSITEiView protein in PROSITE
PS50275 SAC, 1 hit

Sequencei

Sequence statusi: Complete.

P42837-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MNNDAMEHTL GGGILTTSGS KQRKTSKFVM GKYTLYETKD RMYIVGSNKR
60 70 80 90 100
ETMFRILEID LTVPRGELTV LEDNVFFTRN EIMNVLASLE EATEDGLHKK
110 120 130 140 150
ITGYGLLGFI KFTCWYYLIM VTKYSQVAVI GGHGIYHIDG IDIIPITNNY
160 170 180 190 200
KKPEKSSDEA RLLNIFKDLD LTKTFYFSYT YDITNTLQTN ILREKLKAVD
210 220 230 240 250
RCDITIPCGI TDYNEMFVWN NNLLSPIFAC IDTVFDWFQC IIHGFIDQVN
260 270 280 290 300
VSVLGKSIYI TLIARRSHHF AGARFLKRGV NNKGHVANEV ETEQIVTDMI
310 320 330 340 350
LTPFHQPGNG FFDSDRYTSF VQHRGSIPLY WTQDASNLTT KPPIRINVVD
360 370 380 390 400
PFFSPAALHF DNLFQRYGGG TIQILNLIKT KEKTPRETKL LWEFEQCIDY
410 420 430 440 450
LNEFLPTLKK LDYTSWDMSR ASKQDGQGVI EFLEKYAVNT VTTTGIFHNG
460 470 480 490 500
PDFASTKIQE GICRSNCIDC LDRTNAAQFV IGKRALGCQL KSLGIIDNSY
510 520 530 540 550
LEYDSDIVNI LTELFHDLGD TIALQYGGSH LVNTMETYRK INQWSSHSRD
560 570 580 590 600
MIESIKRFYS NSFVDAQRQD AINLFLGHYS WREGFPSLWE MNTDFYLHNA
610 620 630 640 650
YSLNMPKRSY IHWWNDYNIK SVKELINEEL IATGNDVTRE KIIKNVRGYP
660 670 680 690 700
GAFDNYWNEY YLPRSVTWIR DLFAYNMNST RRYHNALSKQ DKAMSPFTSR
710 720 730 740 750
KQSWLNNKLK MITSSKSLEK AEGRVVETTD LDRDTSPKQE LELYEHYLHI
760 770 780 790 800
ISDRSQKLEE KMNSFSYSKY PIFISHESSE IPPMRKVIGE PLVDIAEDFT
810 820 830 840 850
DVYDDDDDGD DENDEMTTEA LLIAPDHVSV DEKFYEKVLN VDDYKPALDD
860 870
YSAVIHIKPD NLQLYRDLCF SKDIQLDFQ
Length:879
Mass (Da):101,746
Last modified:November 1, 1995 - v1
Checksum:i872B3231104185FA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z46259 Genomic DNA Translation: CAA86373.1
Z71601 Genomic DNA Translation: CAA96256.1
BK006947 Genomic DNA Translation: DAA10238.1
PIRiS55864
RefSeqiNP_014074.1, NM_001183163.1

Genome annotation databases

EnsemblFungiiYNL325C; YNL325C; YNL325C
GeneIDi855392
KEGGisce:YNL325C

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z46259 Genomic DNA Translation: CAA86373.1
Z71601 Genomic DNA Translation: CAA96256.1
BK006947 Genomic DNA Translation: DAA10238.1
PIRiS55864
RefSeqiNP_014074.1, NM_001183163.1

3D structure databases

ProteinModelPortaliP42837
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35516, 123 interactors
ComplexPortaliCPX-3088 PAS complex
IntActiP42837, 3 interactors
MINTiP42837
STRINGi4932.YNL325C

PTM databases

iPTMnetiP42837

Proteomic databases

MaxQBiP42837
PaxDbiP42837
PRIDEiP42837

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYNL325C; YNL325C; YNL325C
GeneIDi855392
KEGGisce:YNL325C

Organism-specific databases

SGDiS000005269 FIG4

Phylogenomic databases

GeneTreeiENSGT00550000074943
HOGENOMiHOG000168063
InParanoidiP42837
KOiK22913
OMAiKRKCCAH
OrthoDBiEOG092C1610

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER3O-86
YEAST:MONOMER3O-86
ReactomeiR-SCE-1660514 Synthesis of PIPs at the Golgi membrane
R-SCE-1660516 Synthesis of PIPs at the early endosome membrane
R-SCE-1660517 Synthesis of PIPs at the late endosome membrane

Miscellaneous databases

PROiPR:P42837

Family and domain databases

InterProiView protein in InterPro
IPR002013 SAC_dom
PfamiView protein in Pfam
PF02383 Syja_N, 1 hit
PROSITEiView protein in PROSITE
PS50275 SAC, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiFIG4_YEAST
AccessioniPrimary (citable) accession number: P42837
Secondary accession number(s): D6W0M2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 7, 2018
This is version 144 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
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