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UniProtKB - P42787 (CBPD_DROME)
Protein
Carboxypeptidase D
Gene
svr
Organism
Drosophila melanogaster (Fruit fly)
Status
Functioni
Required for the proper melanization and sclerotization of the cuticle.
3 PublicationsCatalytic activityi
- Releases C-terminal Arg and Lys from polypeptides. EC:3.4.17.22
Cofactori
Zn2+By similarity
pH dependencei
Optimum pH is 7.0 for carboxypeptidase domain 1, and 5.0-6.0 for domain 2.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 101 | Zinc 1; catalyticBy similarity | 1 | |
Metal bindingi | 104 | Zinc 1; catalyticBy similarity | 1 | |
Metal bindingi | 217 | Zinc 1; catalyticBy similarity | 1 | |
Active sitei | 305 | Proton donor/acceptor 1By similarity | 1 | |
Metal bindingi | 517 | Zinc 2; catalyticBy similarity | 1 | |
Metal bindingi | 520 | Zinc 2; catalyticBy similarity | 1 | |
Metal bindingi | 626 | Zinc 2; catalyticBy similarity | 1 | |
Active sitei | 730 | Proton donor/acceptor 2By similarity | 1 |
GO - Molecular functioni
- carboxypeptidase activity Source: FlyBase
- metallocarboxypeptidase activity Source: FlyBase
- structural constituent of cuticle Source: UniProtKB-KW
- zinc ion binding Source: InterPro
GO - Biological processi
- imaginal disc-derived wing morphogenesis Source: FlyBase
- long-term memory Source: FlyBase
- negative regulation of protein phosphorylation Source: UniProtKB
- neuropeptide processing Source: FlyBase
- peptide metabolic process Source: GO_Central
- positive regulation of actin filament polymerization Source: UniProtKB
- protein processing Source: GO_Central
Keywordsi
Molecular function | Carboxypeptidase, Hydrolase, Metalloprotease, Protease |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
BRENDAi | 3.4.17.22, 1994 |
Reactomei | R-DME-977606, Regulation of Complement cascade |
SignaLinki | P42787 |
Protein family/group databases
MEROPSi | M14.037 |
Names & Taxonomyi
Protein namesi | Recommended name: Carboxypeptidase D (EC:3.4.17.22)Alternative name(s): Metallocarboxypeptidase D Protein silver |
Gene namesi | Name:svr Synonyms:CPD, CpepE ORF Names:CG4122 |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0004648, svr |
VEuPathDBi | VectorBase:FBgn0004648 |
Subcellular locationi
Other locations
- Membrane Curated; Single-pass type I membrane protein Curated
Extracellular region or secreted
- extracellular region Source: FlyBase
- extracellular space Source: GO_Central
Other locations
- endomembrane system Source: FlyBase
- integral component of membrane Source: UniProtKB-KW
- perinuclear region of cytoplasm Source: FlyBase
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 26 – 1312 | ExtracellularSequence analysisAdd BLAST | 1287 | |
Transmembranei | 1313 – 1333 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 1334 – 1406 | CytoplasmicSequence analysisAdd BLAST | 73 |
Keywords - Cellular componenti
Cuticle, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 25 | Sequence analysisAdd BLAST | 25 | |
ChainiPRO_0000004405 | 26 – 1406 | Carboxypeptidase DAdd BLAST | 1381 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 133 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 269 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 458 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 549 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 612 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 652 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 787 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 808 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 981 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 1152 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 1251 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Modified residuei | 1380 | Phosphoserine1 Publication | 1 |
Keywords - PTMi
Glycoprotein, PhosphoproteinProteomic databases
PaxDbi | P42787 |
PRIDEi | P42787 |
PTM databases
GlyGeni | P42787, 11 sites |
iPTMneti | P42787 |
Expressioni
Developmental stagei
Embryonic and adult stages.1 Publication
Gene expression databases
Bgeei | FBgn0004648, Expressed in seminal fluid secreting gland and 31 other tissues |
ExpressionAtlasi | P42787, baseline and differential |
Genevisiblei | P42787, DM |
Interactioni
Protein-protein interaction databases
BioGRIDi | 57568, 3 interactors |
IntActi | P42787, 2 interactors |
STRINGi | 7227.FBpp0089126 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 26 – 446 | Domain 1Add BLAST | 421 | |
Regioni | 447 – 865 | Domain 2Add BLAST | 419 | |
Regioni | 866 – 1313 | Domain 3Add BLAST | 448 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 1343 – 1345 | Cell attachment siteSequence analysis | 3 |
Domaini
There are 3 carboxypeptidase-like domains. Only the first two domains seem to have kept a catalytic activity.
Sequence similaritiesi
Belongs to the peptidase M14 family.Curated
Keywords - Domaini
Repeat, Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG2649, Eukaryota |
GeneTreei | ENSGT00940000156919 |
InParanoidi | P42787 |
OMAi | YHEAKKM |
PhylomeDBi | P42787 |
Family and domain databases
InterProi | View protein in InterPro IPR008969, CarboxyPept-like_regulatory IPR000834, Peptidase_M14 |
Pfami | View protein in Pfam PF00246, Peptidase_M14, 2 hits |
PRINTSi | PR00765, CRBOXYPTASEA |
SMARTi | View protein in SMART SM00631, Zn_pept, 2 hits |
SUPFAMi | SSF49464, SSF49464, 4 hits |
PROSITEi | View protein in PROSITE PS00132, CARBOXYPEPT_ZN_1, 2 hits PS00133, CARBOXYPEPT_ZN_2, 2 hits |
s (8+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 8 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 8 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: P42787-1) [UniParc]FASTAAdd to basket
Also known as: B, 1B long tail-1
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MPTLGLLFAS IGIAVLAMGV PHCRGYTIKE DESFLQQPHY ASQEQLEDLF
60 70 80 90 100
AGLEKAYPNQ AKVHFLGRSL EGRNLLALQI SRNTRSRNLL TPPVKYIANM
110 120 130 140 150
HGDETVGRQL LVYMAQYLLG NHERISDLGQ LVNSTDIYLV PTMNPDGYAL
160 170 180 190 200
SQEGNCESLP NYVGRGNAAN IDLNRDFPDR LEQSHVHQLR AQSRQPETAA
210 220 230 240 250
LVNWIVSKPF VLSANFHGGA VVASYPYDNS LAHNECCEES LTPDDRVFKQ
260 270 280 290 300
LAHTYSDNHP IMRKGNNCND SFSGGITNGA HWYELSGGMQ DFNYAFSNCF
310 320 330 340 350
ELTIELSCCK YPAASTLPQE WQRNKASLLQ LLRQAHIGIK GLVTDASGFP
360 370 380 390 400
IADANVYVAG LEEKPMRTSK RGEYWRLLTP GLYSVHASAF GYQTSAPQQV
410 420 430 440 450
RVTNDNQEAL RLDFKLAPVE TNFDGNFRKV KVERSEPPQK LKKQFNGFLT
460 470 480 490 500
PTKYEHHNFT AMESYLRAIS SSYPSLTRLY SIGKSVQGRD LWVLEIFATP
510 520 530 540 550
GSHVPGVPEF KYVANMHGNE VVGKELLLIL TKYMLERYGN DDRITKLVNG
560 570 580 590 600
TRMHFLYSMN PDGYEISIEG DRTGGVGRAN AHGIDLNRNF PDQYGTDRFN
610 620 630 640 650
KVTEPEVAAV MNWTLSLPFV LSANLHGGSL VANYPFDDNE NDFNDPFMRL
660 670 680 690 700
RNSSINGRKP NPTEDNALFK HLAGIYSNAH PTMYLGQPCE LFQNEFFPDG
710 720 730 740 750
ITNGAQWYSV TGGMQDWNYV RAGCLELTIE MGCDKFPKAA ELSRYWEDHR
760 770 780 790 800
EPLLQFIEQV HCGIHGFVHS TIGTPIAGAV VRLDGANHST YSQVFGDYWK
810 820 830 840 850
LALPGRHNLT VLGDNYAPLR MEVEVPDVHP FEMRMDITLM PDDPQHWASA
860 870 880 890 900
NDFRIIENVV NTRYHTNPQV RARLAELENQ NGQIASFGYA DSEFGTIFNY
910 920 930 940 950
LKMTSDIGEP EEHKYKLLVV SSLYDTTAPL GREILLNLIR HLVEGFKLQD
960 970 980 990 1000
TSVVELLKRS VIYFLPQTSK FQNVFDMYNS NTSICDPVLG DELAERILGP
1010 1020 1030 1040 1050
ETDQAKDVFL QFLRSERFDL MLTFGAGNSD LNYPKGDSVL VKFAHRMQRT
1060 1070 1080 1090 1100
EFNYSPLQCP PSATRQLHRE TTERLTNMMY RIYNLPVYTL GISCCRMPHQ
1110 1120 1130 1140 1150
KKIASVWRKN IDKIKNFLAL VKTGVSGLVQ NDKGQPLREA YVRLLEHDRI
1160 1170 1180 1190 1200
INVTKNVARF QLMLPHGLYG LEVTAPNYES QMIKVDVEDG RVTELGIIRM
1210 1220 1230 1240 1250
HPFTLIRGVV LELPNNDNRA TTSIAGVVLD ESNHPVRNAK VSVVGQTQLR
1260 1270 1280 1290 1300
NFTGSMGQYR ISAVPLGTIT LKVEAPRHLE ATRQMHLIQG GLATENVVFH
1310 1320 1330 1340 1350
LKVNEHVFGL PRFLFILCAS VLIIVGVIVC VLCAQFWFYR RHRGDKPYYN
1360 1370 1380 1390 1400
FSLLPQRGKE QFGLEDDDGG DDGETELFRS PIKRELSQRA HLVNNQTNYS
FIIQAA
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketD3DME3 | D3DME3_DROME | LP15968p | svr cc, CG18503, CG32818, CP, CPD | 435 | Annotation score: | ||
X2J9Z1 | X2J9Z1_DROME | Silver, isoform N | svr cc, CG18503, CG32818, CP, CPD | 1,439 | Annotation score: | ||
Q6AWJ5 | Q6AWJ5_DROME | LP12324p | svr cc, CG18503, CG32818, CP, CPD | 433 | Annotation score: |
Sequence cautioni
The sequence AAA91650 differs from that shown. Reason: Frameshift.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 733 | C → Y in AAA91650 (PubMed:7568156).Curated | 1 | |
Sequence conflicti | 1041 | V → E in ABM92809 (Ref. 5) Curated | 1 | |
Sequence conflicti | 1041 | V → E in AAA91650 (PubMed:7568156).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_000773 | 1 – 152 | MPTLG…YALSQ → MLFFCLALIIGCAVGEYSEV RVIQEEDNFLESPHYLKNEE IGDLFSQLAKDYPDLAQTYT IGKSLEDRPIYALALSAPTG ESKNGDLLRPMVKLVANIQG DEAVGRQMVLYMAEYLATHY DGDPKVQALLNLTEIHFLPT CNPDGFAKAK in isoform 3, isoform 4 and isoform 6. 1 PublicationAdd BLAST | 152 | |
Alternative sequenceiVSP_037495 | 2 – 152 | PTLGL…YALSQ → NTCL in isoform 2 and isoform 8. 1 PublicationAdd BLAST | 151 | |
Alternative sequenceiVSP_000775 | 426 – 435 | NFRKVKVERS → ISSFYSPYYF in isoform 6 and isoform 7. 1 Publication | 10 | |
Alternative sequenceiVSP_000776 | 436 – 1406 | Missing in isoform 6 and isoform 7. 1 PublicationAdd BLAST | 971 | |
Alternative sequenceiVSP_000779 | 1385 – 1406 | ELSQR…IIQAA → GMTIQPYFDEEQLERILHTD DDDDDGPHMEPELDVADDSE DDIVMLHNNGNKRRH in isoform 4, isoform 5 and isoform 8. 2 PublicationsAdd BLAST | 22 |
Sequence databases
Genome annotation databases
EnsemblMetazoai | FBtr0070081; FBpp0070080; FBgn0004648 [P42787-1] FBtr0070084; FBpp0089123; FBgn0004648 [P42787-3] FBtr0070085; FBpp0089124; FBgn0004648 [P42787-6] FBtr0070086; FBpp0089125; FBgn0004648 [P42787-7] FBtr0070087; FBpp0089126; FBgn0004648 [P42787-5] FBtr0290016; FBpp0288455; FBgn0004648 [P42787-4] FBtr0290017; FBpp0288456; FBgn0004648 [P42787-8] FBtr0340146; FBpp0309132; FBgn0004648 [P42787-6] FBtr0344737; FBpp0311069; FBgn0004648 [P42787-7] FBtr0344738; FBpp0311070; FBgn0004648 [P42787-1] FBtr0344739; FBpp0311071; FBgn0004648 [P42787-5] |
GeneIDi | 30998 |
KEGGi | dme:Dmel_CG4122 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
3D structure databases
AlphaFoldDBi | P42787 |
SMRi | P42787 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 57568, 3 interactors |
IntActi | P42787, 2 interactors |
STRINGi | 7227.FBpp0089126 |
Protein family/group databases
MEROPSi | M14.037 |
PTM databases
GlyGeni | P42787, 11 sites |
iPTMneti | P42787 |
Proteomic databases
PaxDbi | P42787 |
PRIDEi | P42787 |
Protocols and materials databases
DNASUi | 30998 |
Genome annotation databases
EnsemblMetazoai | FBtr0070081; FBpp0070080; FBgn0004648 [P42787-1] FBtr0070084; FBpp0089123; FBgn0004648 [P42787-3] FBtr0070085; FBpp0089124; FBgn0004648 [P42787-6] FBtr0070086; FBpp0089125; FBgn0004648 [P42787-7] FBtr0070087; FBpp0089126; FBgn0004648 [P42787-5] FBtr0290016; FBpp0288455; FBgn0004648 [P42787-4] FBtr0290017; FBpp0288456; FBgn0004648 [P42787-8] FBtr0340146; FBpp0309132; FBgn0004648 [P42787-6] FBtr0344737; FBpp0311069; FBgn0004648 [P42787-7] FBtr0344738; FBpp0311070; FBgn0004648 [P42787-1] FBtr0344739; FBpp0311071; FBgn0004648 [P42787-5] |
GeneIDi | 30998 |
KEGGi | dme:Dmel_CG4122 |
Organism-specific databases
CTDi | 30998 |
FlyBasei | FBgn0004648, svr |
VEuPathDBi | VectorBase:FBgn0004648 |
Phylogenomic databases
eggNOGi | KOG2649, Eukaryota |
GeneTreei | ENSGT00940000156919 |
InParanoidi | P42787 |
OMAi | YHEAKKM |
PhylomeDBi | P42787 |
Enzyme and pathway databases
BRENDAi | 3.4.17.22, 1994 |
Reactomei | R-DME-977606, Regulation of Complement cascade |
SignaLinki | P42787 |
Miscellaneous databases
BioGRID-ORCSi | 30998, 0 hits in 3 CRISPR screens |
ChiTaRSi | svr, fly |
GenomeRNAii | 30998 |
PROi | PR:P42787 |
Gene expression databases
Bgeei | FBgn0004648, Expressed in seminal fluid secreting gland and 31 other tissues |
ExpressionAtlasi | P42787, baseline and differential |
Genevisiblei | P42787, DM |
Family and domain databases
InterProi | View protein in InterPro IPR008969, CarboxyPept-like_regulatory IPR000834, Peptidase_M14 |
Pfami | View protein in Pfam PF00246, Peptidase_M14, 2 hits |
PRINTSi | PR00765, CRBOXYPTASEA |
SMARTi | View protein in SMART SM00631, Zn_pept, 2 hits |
SUPFAMi | SSF49464, SSF49464, 4 hits |
PROSITEi | View protein in PROSITE PS00132, CARBOXYPEPT_ZN_1, 2 hits PS00133, CARBOXYPEPT_ZN_2, 2 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | CBPD_DROME | |
Accessioni | P42787Primary (citable) accession number: P42787 Secondary accession number(s): A2RVE4 Q9W5F5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | February 21, 2001 | |
Last modified: | May 25, 2022 | |
This is version 197 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - SIMILARITY comments
Index of protein domains and families