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Protein

Lysosomal Pro-X carboxypeptidase

Gene

PRCP

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cleaves C-terminal amino acids linked to proline in peptides such as angiotensin II, III and des-Arg9-bradykinin. This cleavage occurs at acidic pH, but enzymatic activity is retained with some substrates at neutral pH.

Catalytic activityi

Cleavage of a -Pro-|-Xaa bond to release a C-terminal amino acid.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei179Charge relay system1 Publication1
Active sitei430Charge relay system1 Publication1
Active sitei455Charge relay system1 Publication1

GO - Molecular functioni

  • dipeptidyl-peptidase activity Source: GO_Central
  • serine-type carboxypeptidase activity Source: Reactome

GO - Biological processi

Keywordsi

Molecular functionCarboxypeptidase, Hydrolase, Protease

Enzyme and pathway databases

BRENDAi3.4.16.2 2681
ReactomeiR-HSA-140837 Intrinsic Pathway of Fibrin Clot Formation
R-HSA-6798695 Neutrophil degranulation
SIGNORiP42785

Protein family/group databases

ESTHERihuman-PRCP Prolylcarboxypeptidase
MEROPSiS28.001

Names & Taxonomyi

Protein namesi
Recommended name:
Lysosomal Pro-X carboxypeptidase (EC:3.4.16.2)
Alternative name(s):
Angiotensinase C
Lysosomal carboxypeptidase C
Proline carboxypeptidase
Prolylcarboxypeptidase
Short name:
PRCP
Gene namesi
Name:PRCP
Synonyms:PCP
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

EuPathDBiHostDB:ENSG00000137509.10
HGNCiHGNC:9344 PRCP
MIMi176785 gene
neXtProtiNX_P42785

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Lysosome

Pathology & Biotechi

Organism-specific databases

DisGeNETi5547
OpenTargetsiENSG00000137509
PharmGKBiPA33705

Chemistry databases

ChEMBLiCHEMBL2335
GuidetoPHARMACOLOGYi1584

Polymorphism and mutation databases

BioMutaiPRCP
DMDMi1172047

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
PropeptideiPRO_000002730822 – 45Add BLAST24
ChainiPRO_000002730946 – 496Lysosomal Pro-X carboxypeptidaseAdd BLAST451

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi47N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi101N-linked (GlcNAc...) asparagine1 Publication1
Disulfide bondi215 ↔ 3721 Publication
Disulfide bondi233 ↔ 3101 Publication
Disulfide bondi264 ↔ 3431 Publication
Glycosylationi317N-linked (GlcNAc...) asparagine2 Publications1
Glycosylationi336N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi345N-linked (GlcNAc...) asparagine1 Publication1
Disulfide bondi364 ↔ 3941 Publication
Glycosylationi415N-linked (GlcNAc...) asparagine2 Publications1

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

EPDiP42785
MaxQBiP42785
PaxDbiP42785
PeptideAtlasiP42785
PRIDEiP42785
ProteomicsDBi55558

PTM databases

GlyConnecti1478
iPTMnetiP42785
PhosphoSitePlusiP42785

Expressioni

Tissue specificityi

Highest levels in placenta, lung and liver. Also present in heart, brain, pancreas and kidney.

Gene expression databases

BgeeiENSG00000137509 Expressed in 239 organ(s), highest expression level in kidney
CleanExiHS_PRCP
ExpressionAtlasiP42785 baseline and differential
GenevisibleiP42785 HS

Organism-specific databases

HPAiHPA017065

Interactioni

Subunit structurei

Homodimer.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
GPR37L1O608832EBI-2803892,EBI-2927498

Protein-protein interaction databases

BioGridi111538, 27 interactors
IntActiP42785, 8 interactors
STRINGi9606.ENSP00000377055

Chemistry databases

BindingDBiP42785

Structurei

Secondary structure

1496
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP42785
SMRiP42785
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP42785

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni194 – 334SKS domainAdd BLAST141

Sequence similaritiesi

Belongs to the peptidase S28 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2183 Eukaryota
ENOG410Y38A LUCA
GeneTreeiENSGT00530000063027
HOGENOMiHOG000238311
HOVERGENiHBG005526
InParanoidiP42785
KOiK01285
OMAiITDCDIF
OrthoDBiEOG091G056F
PhylomeDBiP42785
TreeFamiTF314414

Family and domain databases

Gene3Di3.40.50.1820, 1 hit
InterProiView protein in InterPro
IPR029058 AB_hydrolase
IPR008758 Peptidase_S28
PfamiView protein in Pfam
PF05577 Peptidase_S28, 1 hit
SUPFAMiSSF53474 SSF53474, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 10 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: P42785-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MGRRALLLLL LSFLAPWATI ALRPALRALG SLHLPTNPTS LPAVAKNYSV
60 70 80 90 100
LYFQQKVDHF GFNTVKTFNQ RYLVADKYWK KNGGSILFYT GNEGDIIWFC
110 120 130 140 150
NNTGFMWDVA EELKAMLVFA EHRYYGESLP FGDNSFKDSR HLNFLTSEQA
160 170 180 190 200
LADFAELIKH LKRTIPGAEN QPVIAIGGSY GGMLAAWFRM KYPHMVVGAL
210 220 230 240 250
AASAPIWQFE DLVPCGVFMK IVTTDFRKSG PHCSESIHRS WDAINRLSNT
260 270 280 290 300
GSGLQWLTGA LHLCSPLTSQ DIQHLKDWIS ETWVNLAMVD YPYASNFLQP
310 320 330 340 350
LPAWPIKVVC QYLKNPNVSD SLLLQNIFQA LNVYYNYSGQ VKCLNISETA
360 370 380 390 400
TSSLGTLGWS YQACTEVVMP FCTNGVDDMF EPHSWNLKEL SDDCFQQWGV
410 420 430 440 450
RPRPSWITTM YGGKNISSHT NIVFSNGELD PWSGGGVTKD ITDTLVAVTI
460 470 480 490
SEGAHHLDLR TKNALDPMSV LLARSLEVRH MKNWIRDFYD SAGKQH
Length:496
Mass (Da):55,800
Last modified:November 1, 1995 - v1
Checksum:iFC5DD570D3D3AB4F
GO
Isoform 2 (identifier: P42785-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     56-56: K → KALAAGQLHICIIQLNHYKTPL

Note: No experimental confirmation available.
Show »
Length:517
Mass (Da):58,100
Checksum:iA2490258A57E6F23
GO

Computationally mapped potential isoform sequencesi

There are 10 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E9PQN3E9PQN3_HUMAN
Lysosomal Pro-X carboxypeptidase
PRCP
183Annotation score:
E9PKN6E9PKN6_HUMAN
Lysosomal Pro-X carboxypeptidase
PRCP
192Annotation score:
E9PNJ1E9PNJ1_HUMAN
Lysosomal Pro-X carboxypeptidase
PRCP
163Annotation score:
E9PQB5E9PQB5_HUMAN
Lysosomal Pro-X carboxypeptidase
PRCP
145Annotation score:
E9PNF7E9PNF7_HUMAN
Lysosomal Pro-X carboxypeptidase
PRCP
116Annotation score:
E9PL85E9PL85_HUMAN
Lysosomal Pro-X carboxypeptidase
PRCP
147Annotation score:
E9PL49E9PL49_HUMAN
Lysosomal Pro-X carboxypeptidase
PRCP
43Annotation score:
E9PR42E9PR42_HUMAN
Lysosomal Pro-X carboxypeptidase
PRCP
26Annotation score:
E9PLY4E9PLY4_HUMAN
Lysosomal Pro-X carboxypeptidase
PRCP
99Annotation score:
E9PIG4E9PIG4_HUMAN
Lysosomal Pro-X carboxypeptidase
PRCP
233Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti104G → E in BAG52417 (PubMed:14702039).Curated1
Sequence conflicti248S → L in BAG52417 (PubMed:14702039).Curated1
Sequence conflicti304W → R in BAG52417 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_020464112E → D1 PublicationCorresponds to variant dbSNP:rs2229437Ensembl.1
Natural variantiVAR_029329444T → S. Corresponds to variant dbSNP:rs2228312Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_04579956K → KALAAGQLHICIIQLNHYKT PL in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L13977 mRNA Translation: AAA99891.1
AK091786 mRNA Translation: BAG52417.1
AK312919 mRNA Translation: BAG35764.1
AB451270 mRNA Translation: BAG70084.1
AB451397 mRNA Translation: BAG70211.1
AP000893 Genomic DNA No translation available.
AP001646 Genomic DNA No translation available.
CH471076 Genomic DNA Translation: EAW75074.1
CH471076 Genomic DNA Translation: EAW75075.1
BC001500 mRNA Translation: AAH01500.1
CCDSiCCDS41695.1 [P42785-2]
CCDS8262.1 [P42785-1]
PIRiA47352
RefSeqiNP_001306143.1, NM_001319214.1
NP_005031.1, NM_005040.3 [P42785-1]
NP_955450.2, NM_199418.3 [P42785-2]
UniGeneiHs.523936
Hs.733148

Genome annotation databases

EnsembliENST00000313010; ENSP00000317362; ENSG00000137509 [P42785-1]
ENST00000393399; ENSP00000377055; ENSG00000137509 [P42785-2]
GeneIDi5547
KEGGihsa:5547
UCSCiuc001ozr.4 human [P42785-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L13977 mRNA Translation: AAA99891.1
AK091786 mRNA Translation: BAG52417.1
AK312919 mRNA Translation: BAG35764.1
AB451270 mRNA Translation: BAG70084.1
AB451397 mRNA Translation: BAG70211.1
AP000893 Genomic DNA No translation available.
AP001646 Genomic DNA No translation available.
CH471076 Genomic DNA Translation: EAW75074.1
CH471076 Genomic DNA Translation: EAW75075.1
BC001500 mRNA Translation: AAH01500.1
CCDSiCCDS41695.1 [P42785-2]
CCDS8262.1 [P42785-1]
PIRiA47352
RefSeqiNP_001306143.1, NM_001319214.1
NP_005031.1, NM_005040.3 [P42785-1]
NP_955450.2, NM_199418.3 [P42785-2]
UniGeneiHs.523936
Hs.733148

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3N2ZX-ray2.79B46-491[»]
ProteinModelPortaliP42785
SMRiP42785
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111538, 27 interactors
IntActiP42785, 8 interactors
STRINGi9606.ENSP00000377055

Chemistry databases

BindingDBiP42785
ChEMBLiCHEMBL2335
GuidetoPHARMACOLOGYi1584

Protein family/group databases

ESTHERihuman-PRCP Prolylcarboxypeptidase
MEROPSiS28.001

PTM databases

GlyConnecti1478
iPTMnetiP42785
PhosphoSitePlusiP42785

Polymorphism and mutation databases

BioMutaiPRCP
DMDMi1172047

Proteomic databases

EPDiP42785
MaxQBiP42785
PaxDbiP42785
PeptideAtlasiP42785
PRIDEiP42785
ProteomicsDBi55558

Protocols and materials databases

DNASUi5547
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000313010; ENSP00000317362; ENSG00000137509 [P42785-1]
ENST00000393399; ENSP00000377055; ENSG00000137509 [P42785-2]
GeneIDi5547
KEGGihsa:5547
UCSCiuc001ozr.4 human [P42785-1]

Organism-specific databases

CTDi5547
DisGeNETi5547
EuPathDBiHostDB:ENSG00000137509.10
GeneCardsiPRCP
HGNCiHGNC:9344 PRCP
HPAiHPA017065
MIMi176785 gene
neXtProtiNX_P42785
OpenTargetsiENSG00000137509
PharmGKBiPA33705
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2183 Eukaryota
ENOG410Y38A LUCA
GeneTreeiENSGT00530000063027
HOGENOMiHOG000238311
HOVERGENiHBG005526
InParanoidiP42785
KOiK01285
OMAiITDCDIF
OrthoDBiEOG091G056F
PhylomeDBiP42785
TreeFamiTF314414

Enzyme and pathway databases

BRENDAi3.4.16.2 2681
ReactomeiR-HSA-140837 Intrinsic Pathway of Fibrin Clot Formation
R-HSA-6798695 Neutrophil degranulation
SIGNORiP42785

Miscellaneous databases

ChiTaRSiPRCP human
EvolutionaryTraceiP42785
GeneWikiiPRCP
GenomeRNAii5547
PROiPR:P42785
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000137509 Expressed in 239 organ(s), highest expression level in kidney
CleanExiHS_PRCP
ExpressionAtlasiP42785 baseline and differential
GenevisibleiP42785 HS

Family and domain databases

Gene3Di3.40.50.1820, 1 hit
InterProiView protein in InterPro
IPR029058 AB_hydrolase
IPR008758 Peptidase_S28
PfamiView protein in Pfam
PF05577 Peptidase_S28, 1 hit
SUPFAMiSSF53474 SSF53474, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPCP_HUMAN
AccessioniPrimary (citable) accession number: P42785
Secondary accession number(s): A8MU24
, B2R7B7, B3KRK5, B5BU34
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 7, 2018
This is version 173 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  7. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
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