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Protein

Chaperone protein ClpB1

Gene

CLPB1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Molecular chaperone that plays an important role in thermotolerance. Together with HSA32, required for long-term acquired thermotolerance (LAT) in plants and naturally high basal thermotolerance observed in germinating seedlings.7 Publications

Miscellaneous

Over-expression of HSP101 partially reverse the sensitivity of 14 day-old seedling to heat stress.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi207 – 214ATPSequence analysis8
Nucleotide bindingi606 – 613ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

  • positive regulation of translation Source: TAIR
  • protein metabolic process Source: InterPro
  • protein unfolding Source: TAIR
  • response to heat Source: TAIR
  • response to high light intensity Source: TAIR
  • response to hydrogen peroxide Source: TAIR

Keywordsi

Molecular functionChaperone
Biological processStress response
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Chaperone protein ClpB1
Alternative name(s):
ATP-dependent Clp protease ATP-binding subunit ClpB homolog 1
Casein lytic proteinase B1
Heat shock protein 101
Protein DEFECTIVE IN LONG-TERM ACQUIRED THERMOTOLERANCE1 Publication
Gene namesi
Name:CLPB1
Synonyms:DLT11 Publication, HOT1, HSP101
Ordered Locus Names:At1g74310
ORF Names:F1O17.2
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G74310
TAIRilocus:2019667 AT1G74310

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

No visible phenotype under normal growth conditions, but germinating seeds have greatly reduced basal thermotolerance and are unable to acquire thermotolerance (PubMed:10760305, PubMed:11489180). Faster degradation of HSA32 (at protein level) (PubMed:23439916).3 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi33H → Y in dlt1-2; normal growth and development under nonstress conditions. Impaired chaperone activity and thermotolerance function, but normal positive regulation of HSA32 during recovery after heat treatment. 1 Publication1
Mutagenesisi499A → T in hot1-4; reduced basal thermotolerance and unable to acquire thermotolerance. 1 Publication1
Mutagenesisi509E → K in hot1-6; reduced ability to acquire thermotolerance. 1 Publication1
Mutagenesisi599T → I in dlt1-1; normal growth and development under nonstress conditions, and normal chaperone activity and thermotolerance function. Compromised positive regulation of HSA32 during recovery after heat treatment. 1 Publication1
Mutagenesisi637E → K in hot1-1; greatly reduced basal thermotolerance and unable to acquire thermotolerance. 2 Publications1
Mutagenesisi706R → K in hot1-5; unable to acquire thermotolerance. 1 Publication1
Mutagenesisi815G → D in hot1-7; reduced ability to acquire thermotolerance. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001912201 – 911Chaperone protein ClpB1Add BLAST911

Proteomic databases

PaxDbiP42730
PRIDEiP42730

Expressioni

Developmental stagei

Starts to accumulate in maturing seeds 15 days after pollination and decreases rapidly during seed germination.2 Publications

Inductioni

By heat stress and oilseed rape mosaic virus (PubMed:10760238, PubMed:10760305, PubMed:11489180, PubMed:16644052, PubMed:16995899, PubMed:17144892, PubMed:7866032). Regulated by HSA32 that retards its decay in a positive feedback loop (at protein level) during recovery after heat treatment (PubMed:23439916).8 Publications

Gene expression databases

ExpressionAtlasiP42730 differential
GenevisibleiP42730 AT

Interactioni

Protein-protein interaction databases

BioGridi28990, 2 interactors
IntActiP42730, 1 interactor
STRINGi3702.AT1G74310.1

Structurei

3D structure databases

ProteinModelPortaliP42730
SMRiP42730
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni164 – 410IAdd BLAST247
Regioni532 – 723IIAdd BLAST192

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili403 – 500Sequence analysisAdd BLAST98

Sequence similaritiesi

Belongs to the ClpA/ClpB family.Curated

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiKOG1051 Eukaryota
COG0542 LUCA
HOGENOMiHOG000218211
InParanoidiP42730
KOiK03695
OMAiEIRVEMN
OrthoDBiEOG093602DK
PhylomeDBiP42730

Family and domain databases

Gene3Di1.10.1780.10, 1 hit
InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR003959 ATPase_AAA_core
IPR019489 Clp_ATPase_C
IPR004176 Clp_N
IPR036628 Clp_N_dom_sf
IPR001270 ClpA/B
IPR018368 ClpA/B_CS1
IPR028299 ClpA/B_CS2
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF00004 AAA, 1 hit
PF07724 AAA_2, 1 hit
PF02861 Clp_N, 2 hits
PF10431 ClpB_D2-small, 1 hit
PRINTSiPR00300 CLPPROTEASEA
SMARTiView protein in SMART
SM00382 AAA, 2 hits
SM01086 ClpB_D2-small, 1 hit
SUPFAMiSSF52540 SSF52540, 2 hits
SSF81923 SSF81923, 1 hit
PROSITEiView protein in PROSITE
PS00870 CLPAB_1, 1 hit
PS00871 CLPAB_2, 1 hit

Sequencei

Sequence statusi: Complete.

P42730-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNPEKFTHKT NETIATAHEL AVNAGHAQFT PLHLAGALIS DPTGIFPQAI
60 70 80 90 100
SSAGGENAAQ SAERVINQAL KKLPSQSPPP DDIPASSSLI KVIRRAQAAQ
110 120 130 140 150
KSRGDTHLAV DQLIMGLLED SQIRDLLNEV GVATARVKSE VEKLRGKEGK
160 170 180 190 200
KVESASGDTN FQALKTYGRD LVEQAGKLDP VIGRDEEIRR VVRILSRRTK
210 220 230 240 250
NNPVLIGEPG VGKTAVVEGL AQRIVKGDVP NSLTDVRLIS LDMGALVAGA
260 270 280 290 300
KYRGEFEERL KSVLKEVEDA EGKVILFIDE IHLVLGAGKT EGSMDAANLF
310 320 330 340 350
KPMLARGQLR CIGATTLEEY RKYVEKDAAF ERRFQQVYVA EPSVPDTISI
360 370 380 390 400
LRGLKEKYEG HHGVRIQDRA LINAAQLSAR YITGRHLPDK AIDLVDEACA
410 420 430 440 450
NVRVQLDSQP EEIDNLERKR MQLEIELHAL EREKDKASKA RLIEVRKELD
460 470 480 490 500
DLRDKLQPLT MKYRKEKERI DEIRRLKQKR EELMFSLQEA ERRYDLARAA
510 520 530 540 550
DLRYGAIQEV ESAIAQLEGT SSEENVMLTE NVGPEHIAEV VSRWTGIPVT
560 570 580 590 600
RLGQNEKERL IGLADRLHKR VVGQNQAVNA VSEAILRSRA GLGRPQQPTG
610 620 630 640 650
SFLFLGPTGV GKTELAKALA EQLFDDENLL VRIDMSEYME QHSVSRLIGA
660 670 680 690 700
PPGYVGHEEG GQLTEAVRRR PYCVILFDEV EKAHVAVFNT LLQVLDDGRL
710 720 730 740 750
TDGQGRTVDF RNSVIIMTSN LGAEHLLAGL TGKVTMEVAR DCVMREVRKH
760 770 780 790 800
FRPELLNRLD EIVVFDPLSH DQLRKVARLQ MKDVAVRLAE RGVALAVTDA
810 820 830 840 850
ALDYILAESY DPVYGARPIR RWMEKKVVTE LSKMVVREEI DENSTVYIDA
860 870 880 890 900
GAGDLVYRVE SGGLVDASTG KKSDVLIHIA NGPKRSDAAQ AVKKMRIEEI
910
EDDDNEEMIE D
Length:911
Mass (Da):101,295
Last modified:May 27, 2002 - v2
Checksum:i191EC1853B0C4CB9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti141V → F in AAL32674 (PubMed:14593172).Curated1
Sequence conflicti595P → A in AAA67927 (PubMed:7866032).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U13949 mRNA Translation: AAA67927.1
AF218796 Genomic DNA Translation: AAF26423.1
AC020579 Genomic DNA Translation: AAG52410.1
CP002684 Genomic DNA Translation: AEE35576.1
AY062596 mRNA Translation: AAL32674.1
PIRiF96771
RefSeqiNP_565083.1, NM_106091.4
UniGeneiAt.48370
At.69978

Genome annotation databases

EnsemblPlantsiAT1G74310.1; AT1G74310.1; AT1G74310
GeneIDi843771
GrameneiAT1G74310.1; AT1G74310.1; AT1G74310
KEGGiath:AT1G74310

Similar proteinsi

Entry informationi

Entry nameiCLPB1_ARATH
AccessioniPrimary (citable) accession number: P42730
Secondary accession number(s): Q8W4F2, Q9LE57
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: May 27, 2002
Last modified: May 23, 2018
This is version 136 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

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