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Protein

Plastocyanin major isoform, chloroplastic

Gene

DRT112

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Participates in electron transfer between P700 and the cytochrome b6-f complex in photosystem I. Seems to be the major plastocyanin in Arabidopsis.1 Publication

Caution

Was originally thought to be involved in the resistance to UV light and chemical DNA-damaging agents.1 Publication

Cofactori

Cu2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi105Copper; via pros nitrogenBy similarity1
Metal bindingi152CopperBy similarity1
Metal bindingi155Copper; via pros nitrogenBy similarity1
Metal bindingi160CopperBy similarity1

GO - Molecular functioni

  • copper ion binding Source: InterPro
  • electron transporter, transferring electrons from cytochrome b6/f complex of photosystem II activity Source: CACAO
  • protein domain specific binding Source: CAFA

GO - Biological processi

  • copper ion homeostasis Source: TAIR
  • negative regulation of translation Source: TAIR
  • response to copper ion Source: TAIR

Keywordsi

Biological processElectron transport, Transport
LigandCopper, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Plastocyanin major isoform, chloroplastic
Alternative name(s):
DNA-damage-repair/toleration protein DRT112
Gene namesi
Name:DRT112
Ordered Locus Names:At1g20340
ORF Names:F14O10.6, F14O10_4, K3F10TP
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G20340
TAIRilocus:2012928 AT1G20340

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Membrane, Plastid, Thylakoid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 52ChloroplastSequence analysisAdd BLAST52
Transit peptidei53 – 68Thylakoid3 PublicationsAdd BLAST16
ChainiPRO_000000288569 – 167Plastocyanin major isoform, chloroplasticAdd BLAST99

Proteomic databases

PaxDbiP42699
PRIDEiP42699

Expressioni

Gene expression databases

ExpressionAtlasiP42699 baseline and differential
GenevisibleiP42699 AT

Interactioni

GO - Molecular functioni

  • protein domain specific binding Source: CAFA

Protein-protein interaction databases

BioGridi23861, 1 interactor
STRINGi3702.AT1G20340.1

Structurei

3D structure databases

ProteinModelPortaliP42699
SMRiP42699
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini69 – 167Plastocyanin-likeAdd BLAST99

Sequence similaritiesi

Belongs to the plastocyanin family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiENOG410IY43 Eukaryota
COG3794 LUCA
HOGENOMiHOG000209447
InParanoidiP42699
KOiK02638
OMAiNNKLAPH
OrthoDBiEOG09360R7Y
PhylomeDBiP42699

Family and domain databases

CDDicd04219 Plastocyanin, 1 hit
Gene3Di2.60.40.420, 1 hit
InterProiView protein in InterPro
IPR000923 BlueCu_1
IPR028871 BlueCu_1_BS
IPR001235 Copper_blue_Plastocyanin
IPR008972 Cupredoxin
IPR002387 Plastocyanin
PfamiView protein in Pfam
PF00127 Copper-bind, 1 hit
PRINTSiPR00156 COPPERBLUE
PR00157 PLASTOCYANIN
SUPFAMiSSF49503 SSF49503, 1 hit
TIGRFAMsiTIGR02656 cyanin_plasto, 1 hit
PROSITEiView protein in PROSITE
PS00196 COPPER_BLUE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P42699-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASVTSATVA IPSFTGLKAS TIKSSATVRI QTAAVASPKL TVKSSLKNFG
60 70 80 90 100
VAAVAAAASI ALAGNAMAIE VLLGGGDGSL AFIPNDFSIA KGEKIVFKNN
110 120 130 140 150
AGYPHNVVFD EDEIPSGVDV AKISMDEQDL LNGAGETYEV ALTEPGTYSF
160
YCAPHQGAGM VGKVTVN
Length:167
Mass (Da):16,984
Last modified:December 1, 2000 - v2
Checksum:i00BE1D33D47E35D6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti7A → T in AAA32787 (PubMed:8479917).Curated1
Sequence conflicti10A → P in AAA32787 (PubMed:8479917).Curated1
Sequence conflicti22I → T in AAA32787 (PubMed:8479917).Curated1
Sequence conflicti30I → T in AAA32787 (PubMed:8479917).Curated1
Sequence conflicti56A → P in AAA32787 (PubMed:8479917).Curated1
Sequence conflicti164V → I in AAM64356 (Ref. 6) Curated1
Sequence conflicti166V → G in CAB66894 (PubMed:11034343).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M98456 mRNA Translation: AAA32787.1
AJ271355 Genomic DNA Translation: CAB66894.1
AC026234 Genomic DNA Translation: AAF88155.1
CP002684 Genomic DNA Translation: AEE29963.1
AF324702 mRNA Translation: AAG40053.1
AF326879 mRNA Translation: AAG41461.1
AF334383 mRNA Translation: AAG50089.1
AF361853 mRNA Translation: AAK32865.1
BT001030 mRNA Translation: AAN46784.1
AY086284 mRNA Translation: AAM64356.1
AY374308 Genomic DNA Translation: AAR15395.1
PIRiB86337
PA0004
S33707
RefSeqiNP_173459.1, NM_101885.3
UniGeneiAt.24399
At.71481
At.71708
At.72031

Genome annotation databases

EnsemblPlantsiAT1G20340.1; AT1G20340.1; AT1G20340
GeneIDi838622
GrameneiAT1G20340.1; AT1G20340.1; AT1G20340
KEGGiath:AT1G20340

Similar proteinsi

Entry informationi

Entry nameiPLAS2_ARATH
AccessioniPrimary (citable) accession number: P42699
Secondary accession number(s): Q6UB15, Q8LD06, Q9LN26
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: December 1, 2000
Last modified: May 23, 2018
This is version 143 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

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