UniProtKB - P42683 (LCK_CHICK)
Protein
Proto-oncogene tyrosine-protein kinase LCK
Gene
LCK
Organism
Gallus gallus (Chicken)
Status
Functioni
Tyrosine kinase that plays an essential role for the selection and maturation of developing T-cell in the thymus and in mature T-cell function. Is constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors and plays a key role in T-cell antigen receptor(TCR)-linked signal transduction pathways (By similarity).By similarity
Catalytic activityi
- EC:2.7.10.2PROSITE-ProRule annotation
Activity regulationi
Inhibited by tyrosine phosphorylation.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 272 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 363 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 250 – 258 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- non-membrane spanning protein tyrosine kinase activity Source: UniProtKB-EC
- protein serine/threonine phosphatase activity Source: UniProtKB
- protein tyrosine kinase activity Source: UniProtKB
- SH2 domain binding Source: UniProtKB
GO - Biological processi
- activation of cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB
- cellular zinc ion homeostasis Source: UniProtKB
- positive regulation of intrinsic apoptotic signaling pathway Source: UniProtKB
- positive regulation of T cell activation Source: UniProtKB
- response to drug Source: UniProtKB
- T cell differentiation Source: UniProtKB
Keywordsi
Molecular function | Kinase, Transferase, Tyrosine-protein kinase |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 2.7.10.2, 1306 |
Names & Taxonomyi
Protein namesi | Recommended name: Proto-oncogene tyrosine-protein kinase LCK (EC:2.7.10.2)Alternative name(s): Protein-tyrosine kinase C-TKL p56tk1 |
Gene namesi | Name:LCK |
Organismi | Gallus gallus (Chicken) |
Taxonomic identifieri | 9031 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archelosauria › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galloanserae › Galliformes › Phasianidae › Phasianinae › Gallus |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Lipid-anchor By similarity; Cytoplasmic side By similarity
Cytosol
- cytosol By similarity
Cytoskeleton
- pericentriolar material Source: UniProtKB
Cytosol
- cytosol Source: UniProtKB-SubCell
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
- membrane raft Source: UniProtKB
Keywords - Cellular componenti
Cell membrane, Cytoplasm, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCurated | |||
ChainiPRO_0000088128 | 2 – 508 | Proto-oncogene tyrosine-protein kinase LCKAdd BLAST | 507 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 2 | N-myristoyl glycineBy similarity | 1 | |
Lipidationi | 3 | S-palmitoyl cysteineBy similarity | 1 | |
Lipidationi | 5 | S-palmitoyl cysteineBy similarity | 1 | |
Modified residuei | 393 | Phosphotyrosine; by autocatalysisBy similarity | 1 | |
Modified residuei | 504 | PhosphotyrosineBy similarity | 1 |
Post-translational modificationi
Phosphorylated on Tyr-393, which increases enzymatic activity, this site is dephosphorylated by PTN22. Phosphorylated on Tyr-504, presumably by CSK, which decreases activity. Dephosphorylated by PTPRC/CD45. Dephosphorylation at Tyr-393 by PTPN2 negatively regulates T-cells differentiation (By similarity).By similarity
Palmitoylation regulates association with the plasma membrane.By similarity
Keywords - PTMi
Lipoprotein, Myristate, Palmitate, PhosphoproteinPTM databases
iPTMneti | P42683 |
Interactioni
Subunit structurei
Binds to the cytoplasmic domain of cell surface receptors, such as CD4, CD8.
By similarityGO - Molecular functioni
- SH2 domain binding Source: UniProtKB
Protein-protein interaction databases
STRINGi | 9031.ENSGALP00000000634 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 60 – 120 | SH3PROSITE-ProRule annotationAdd BLAST | 61 | |
Domaini | 126 – 223 | SH2PROSITE-ProRule annotationAdd BLAST | 98 | |
Domaini | 244 – 497 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 254 |
Sequence similaritiesi
Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.PROSITE-ProRule annotation
Keywords - Domaini
SH2 domain, SH3 domainPhylogenomic databases
eggNOGi | KOG0197, Eukaryota |
InParanoidi | P42683 |
PhylomeDBi | P42683 |
Family and domain databases
CDDi | cd10362, SH2_Src_Lck, 1 hit cd12005, SH3_Lck, 1 hit |
Gene3Di | 3.30.505.10, 1 hit |
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR035850, Lck_SH2 IPR035749, Lck_SH3 IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR001245, Ser-Thr/Tyr_kinase_cat_dom IPR000980, SH2 IPR036860, SH2_dom_sf IPR036028, SH3-like_dom_sf IPR001452, SH3_domain IPR008266, Tyr_kinase_AS IPR020635, Tyr_kinase_cat_dom |
Pfami | View protein in Pfam PF07714, PK_Tyr_Ser-Thr, 1 hit PF00017, SH2, 1 hit PF00018, SH3_1, 1 hit |
PRINTSi | PR00401, SH2DOMAIN PR00452, SH3DOMAIN PR00109, TYRKINASE |
SMARTi | View protein in SMART SM00252, SH2, 1 hit SM00326, SH3, 1 hit SM00219, TyrKc, 1 hit |
SUPFAMi | SSF50044, SSF50044, 1 hit SSF55550, SSF55550, 1 hit SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00109, PROTEIN_KINASE_TYR, 1 hit PS50001, SH2, 1 hit PS50002, SH3, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P42683-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGCCCSSDYD EDWIENIDIC EHCNYPIDPD SKRQQLIRNV SEVRDPLVSY
60 70 80 90 100
EAMSPPCSPL QDKLVVALYD YEPTHDGDLG LKQGEKLRVL EESGEWWRAQ
110 120 130 140 150
SLTTGQEGLI PHNFVAMVNS LEPEPWFFKN LSRKNAEARL LASGNTHGSF
160 170 180 190 200
LIRESETSKG SYSLSVRDFD QNQGETVKHY KIRNMDNGGY YISPRVTFSS
210 220 230 240 250
LHELVEYYSS SSDGLCTRLG KPCRTQKPQK PWWQDEWEVP RESLKLVEKL
260 270 280 290 300
GAGQFGEVWM GFYNGHTKVA IKNLKQGSMS PSAFLAEANL MKNLQHPRLV
310 320 330 340 350
RLYAVVTKEP IYIITEYMEK GSLVDFLKTS EGIKLSINKL LDMAAQIAEG
360 370 380 390 400
MAFIEAKNYI HRDLRAANIL VSEALCCKIA DFGLARLIED NEYTAREGAK
410 420 430 440 450
FPIKWTAPEA INYGTFTIKS DVWSFGILLT EIVTYGRIPY PGMTNPEVIQ
460 470 480 490 500
NLERGYRMPQ PDNCPQELYE LMMQCWKEQP EERPTFEYMK SVLEDFFTAT
EGQYQQQP
Sequence cautioni
The sequence AAA49081 differs from that shown. Reason: Erroneous initiation.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X60380 mRNA Translation: CAA42930.1 M85043 mRNA Translation: AAA49003.1 J03579 mRNA Translation: AAA49081.1 Different initiation. |
PIRi | A42126, A39939 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X60380 mRNA Translation: CAA42930.1 M85043 mRNA Translation: AAA49003.1 J03579 mRNA Translation: AAA49081.1 Different initiation. |
PIRi | A42126, A39939 |
3D structure databases
SMRi | P42683 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9031.ENSGALP00000000634 |
PTM databases
iPTMneti | P42683 |
Phylogenomic databases
eggNOGi | KOG0197, Eukaryota |
InParanoidi | P42683 |
PhylomeDBi | P42683 |
Enzyme and pathway databases
BRENDAi | 2.7.10.2, 1306 |
Family and domain databases
CDDi | cd10362, SH2_Src_Lck, 1 hit cd12005, SH3_Lck, 1 hit |
Gene3Di | 3.30.505.10, 1 hit |
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR035850, Lck_SH2 IPR035749, Lck_SH3 IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR001245, Ser-Thr/Tyr_kinase_cat_dom IPR000980, SH2 IPR036860, SH2_dom_sf IPR036028, SH3-like_dom_sf IPR001452, SH3_domain IPR008266, Tyr_kinase_AS IPR020635, Tyr_kinase_cat_dom |
Pfami | View protein in Pfam PF07714, PK_Tyr_Ser-Thr, 1 hit PF00017, SH2, 1 hit PF00018, SH3_1, 1 hit |
PRINTSi | PR00401, SH2DOMAIN PR00452, SH3DOMAIN PR00109, TYRKINASE |
SMARTi | View protein in SMART SM00252, SH2, 1 hit SM00326, SH3, 1 hit SM00219, TyrKc, 1 hit |
SUPFAMi | SSF50044, SSF50044, 1 hit SSF55550, SSF55550, 1 hit SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00109, PROTEIN_KINASE_TYR, 1 hit PS50001, SH2, 1 hit PS50002, SH3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | LCK_CHICK | |
Accessioni | P42683Primary (citable) accession number: P42683 Secondary accession number(s): Q53WS8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | January 23, 2007 | |
Last modified: | December 2, 2020 | |
This is version 141 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families