UniProtKB - P42669 (PURA_MOUSE)
Protein
Transcriptional activator protein Pur-alpha
Gene
Pura
Organism
Mus musculus (Mouse)
Status
Functioni
This is a probable transcription activator that specifically binds the purine-rich single strand of the PUR element located upstream of the c-Myc gene. May play a role in the initiation of DNA replication and in recombination.
GO - Molecular functioni
- DNA binding Source: MGI
- DNA-binding transcription factor activity Source: MGI
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
- double-stranded DNA binding Source: MGI
- double-stranded telomeric DNA binding Source: MGI
- purine-rich negative regulatory element binding Source: HGNC-UCL
- RNA binding Source: InterPro
- RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: GO_Central
- single-stranded DNA binding Source: UniProtKB
- SMAD binding Source: MGI
- transcription factor binding Source: UniProtKB
- translation repressor activity, mRNA regulatory element binding Source: HGNC-UCL
GO - Biological processi
- cell differentiation Source: InterPro
- dendritic transport of messenger ribonucleoprotein complex Source: SynGO
- DNA unwinding involved in DNA replication Source: MGI
- epithelial cell proliferation Source: MGI
- lymphocyte proliferation Source: MGI
- negative regulation of transcription, DNA-templated Source: HGNC-UCL
- negative regulation of transcription by RNA polymerase II Source: UniProtKB
- nervous system development Source: MGI
- positive regulation of cell population proliferation Source: MGI
- regulation of transcription by RNA polymerase II Source: GO_Central
Keywordsi
Molecular function | Activator, DNA-binding |
Biological process | Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: Transcriptional activator protein Pur-alphaAlternative name(s): Purine-rich single-stranded DNA-binding protein alpha |
Gene namesi | Name:Pura |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:103079, Pura |
Subcellular locationi
Nucleus
Nucleus
- nucleus Source: MGI
Other locations
- cytoplasm Source: MGI
- dendrite Source: MGI
- dendrite cytoplasm Source: GOC
- glutamatergic synapse Source: SynGO
- neuronal cell body Source: MGI
- postsynapse Source: SynGO
- postsynaptic density Source: MGI
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000097108 | 2 – 321 | Transcriptional activator protein Pur-alphaAdd BLAST | 320 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineCombined sources | 1 | |
Modified residuei | 181 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | P42669 |
jPOSTi | P42669 |
MaxQBi | P42669 |
PaxDbi | P42669 |
PeptideAtlasi | P42669 |
PRIDEi | P42669 |
ProteomicsDBi | 301890 |
2D gel databases
UCD-2DPAGEi | P42669 |
PTM databases
iPTMneti | P42669 |
PhosphoSitePlusi | P42669 |
SwissPalmi | P42669 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000043991, Expressed in striatum and 312 other tissues |
Genevisiblei | P42669, MM |
Interactioni
Subunit structurei
GO - Molecular functioni
- SMAD binding Source: MGI
- transcription factor binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 202515, 30 interactors |
IntActi | P42669, 16 interactors |
MINTi | P42669 |
STRINGi | 10090.ENSMUSP00000059404 |
Miscellaneous databases
RNActi | P42669, protein |
Family & Domainsi
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 11 – 52 | Gly-richAdd BLAST | 42 | |
Compositional biasi | 292 – 321 | Gln/Glu-rich (part of the transcriptional activation domain)Add BLAST | 30 |
Sequence similaritiesi
Belongs to the PUR DNA-binding protein family.Curated
Phylogenomic databases
eggNOGi | KOG3074, Eukaryota |
GeneTreei | ENSGT00950000183162 |
HOGENOMi | CLU_057873_1_1_1 |
InParanoidi | P42669 |
OMAi | MSSWGAF |
OrthoDBi | 1248813at2759 |
PhylomeDBi | P42669 |
TreeFami | TF313701 |
Family and domain databases
InterProi | View protein in InterPro IPR006628, PUR-bd_fam IPR030500, PURalpha |
PANTHERi | PTHR12611, PTHR12611, 1 hit PTHR12611:SF2, PTHR12611:SF2, 1 hit |
Pfami | View protein in Pfam PF04845, PurA, 1 hit |
SMARTi | View protein in SMART SM00712, PUR, 3 hits |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P42669-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MADRDSGSEQ GGAALGSGGS LGHPGSGSGS GGGGGGGGGG GGSGGGGGAP
60 70 80 90 100
GGLQHETQEL ASKRVDIQNK RFYLDVKQNA KGRFLKIAEV GAGGNKSRLT
110 120 130 140 150
LSMSVAVEFR DYLGDFIEHY AQLGPSQPPD LAQAQDEPRR ALKSEFLVRE
160 170 180 190 200
NRKYYMDLKE NQRGRFLRIR QTVNRGPGLG STQGQTIALP AQGLIEFRDA
210 220 230 240 250
LAKLIDDYGV EEEPAELPEG TSLTVDNKRF FFDVGSNKYG VFMRVSEVKP
260 270 280 290 300
TYRNSITVPY KVWAKFGHTF CKYSEEMKKI QEKQREKRAA CEQLHQQQQQ
310 320
QQEETTAATL LLQGEEEGEE D
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U02098 mRNA Translation: AAA64630.1 AF017631 mRNA Translation: AAB71860.1 |
CCDSi | CCDS29151.1 |
RefSeqi | NP_033015.1, NM_008989.3 XP_006525787.1, XM_006525724.3 XP_011245162.1, XM_011246860.2 |
Genome annotation databases
Ensembli | ENSMUST00000051301; ENSMUSP00000059404; ENSMUSG00000043991 |
GeneIDi | 19290 |
KEGGi | mmu:19290 |
UCSCi | uc008enh.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U02098 mRNA Translation: AAA64630.1 AF017631 mRNA Translation: AAB71860.1 |
CCDSi | CCDS29151.1 |
RefSeqi | NP_033015.1, NM_008989.3 XP_006525787.1, XM_006525724.3 XP_011245162.1, XM_011246860.2 |
3D structure databases
SMRi | P42669 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 202515, 30 interactors |
IntActi | P42669, 16 interactors |
MINTi | P42669 |
STRINGi | 10090.ENSMUSP00000059404 |
PTM databases
iPTMneti | P42669 |
PhosphoSitePlusi | P42669 |
SwissPalmi | P42669 |
2D gel databases
UCD-2DPAGEi | P42669 |
Proteomic databases
EPDi | P42669 |
jPOSTi | P42669 |
MaxQBi | P42669 |
PaxDbi | P42669 |
PeptideAtlasi | P42669 |
PRIDEi | P42669 |
ProteomicsDBi | 301890 |
Protocols and materials databases
Antibodypediai | 26834, 260 antibodies |
DNASUi | 19290 |
Genome annotation databases
Ensembli | ENSMUST00000051301; ENSMUSP00000059404; ENSMUSG00000043991 |
GeneIDi | 19290 |
KEGGi | mmu:19290 |
UCSCi | uc008enh.2, mouse |
Organism-specific databases
CTDi | 5813 |
MGIi | MGI:103079, Pura |
Phylogenomic databases
eggNOGi | KOG3074, Eukaryota |
GeneTreei | ENSGT00950000183162 |
HOGENOMi | CLU_057873_1_1_1 |
InParanoidi | P42669 |
OMAi | MSSWGAF |
OrthoDBi | 1248813at2759 |
PhylomeDBi | P42669 |
TreeFami | TF313701 |
Miscellaneous databases
BioGRID-ORCSi | 19290, 2 hits in 52 CRISPR screens |
ChiTaRSi | Pura, mouse |
PROi | PR:P42669 |
RNActi | P42669, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000043991, Expressed in striatum and 312 other tissues |
Genevisiblei | P42669, MM |
Family and domain databases
InterProi | View protein in InterPro IPR006628, PUR-bd_fam IPR030500, PURalpha |
PANTHERi | PTHR12611, PTHR12611, 1 hit PTHR12611:SF2, PTHR12611:SF2, 1 hit |
Pfami | View protein in Pfam PF04845, PurA, 1 hit |
SMARTi | View protein in SMART SM00712, PUR, 3 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PURA_MOUSE | |
Accessioni | P42669Primary (citable) accession number: P42669 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | November 1, 1995 | |
Last modified: | April 7, 2021 | |
This is version 169 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families