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Entry version 138 (05 Jun 2019)
Sequence version 2 (11 Oct 2004)
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Protein

PFL-like enzyme TdcE

Gene

tdcE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Catalyzes the cleavage of 2-ketobutyrate to propionyl-CoA and formate. It can also use pyruvate as substrate.1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes regulatory mechanisms for enzymes, transporters or microbial transcription factors, and reports the components which regulate (by activation or inhibition) the reaction.<p><a href='/help/activity_regulation' target='_top'>More...</a></p>Activity regulationi

Dependent on PFL-activase.1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: L-threonine degradation via propanoate pathway

This protein is involved in step 2 of the subpathway that synthesizes propanoate from L-threonine.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. L-threonine dehydratase catabolic TdcB (tdcB)
  2. PFL-like enzyme TdcE (tdcE)
  3. no protein annotated in this organism
  4. Propionate kinase (tdcD)
This subpathway is part of the pathway L-threonine degradation via propanoate pathway, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes propanoate from L-threonine, the pathway L-threonine degradation via propanoate pathway and in Amino-acid degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei423S-acetylcysteine intermediateBy similarity1
Active sitei424Cysteine radical intermediateBy similarity1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

  • 2-ketobutyrate formate-lyase activity Source: EcoliWiki
  • formate C-acetyltransferase activity Source: EcoCyc

GO - Biological processi

  • carbohydrate metabolic process Source: InterPro
  • L-threonine catabolic process to propionate Source: EcoliWiki
  • threonine catabolic process Source: EcoCyc

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionAcyltransferase, Transferase

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
EcoCyc:KETOBUTFORMLY-INACT-MONOMER
ECOL316407:JW5522-MONOMER
MetaCyc:KETOBUTFORMLY-INACT-MONOMER

UniPathway: a resource for the exploration and annotation of metabolic pathways

More...
UniPathwayi
UPA00052;UER00508

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
PFL-like enzyme TdcE
Alternative name(s):
Keto-acid formate acetyltransferase
Keto-acid formate-lyase
Ketobutyrate formate-lyase (EC:2.3.1.-)
Short name:
KFL
Pyruvate formate-lyase (EC:2.3.1.54)
Short name:
PFL
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:tdcE
Synonyms:yhaS
Ordered Locus Names:b3114, JW5522
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiEscherichia coli (strain K12)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri83333 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000318 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

Escherichia coli strain K12 genome database

More...
EcoGenei
EG12758 tdcE

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This subsection of the ‘Pathology and Biotech’ section describes the in vivo effects caused by ablation of the gene (or one or more transcripts) coding for the protein described in the entry. This includes gene knockout and knockdown, provided experiments have been performed in the context of a whole organism or a specific tissue, and not at the single-cell level.<p><a href='/help/disruption_phenotype' target='_top'>More...</a></p>Disruption phenotypei

No discernible phenotype.1 Publication

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001666911 – 764PFL-like enzyme TdcEAdd BLAST764

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei739Glycine radicalPROSITE-ProRule annotation1

Keywords - PTMi

Organic radical

Proteomic databases

jPOST - Japan Proteome Standard Repository/Database

More...
jPOSTi
P42632

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
P42632

PRoteomics IDEntifications database

More...
PRIDEi
P42632

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the ‘Expression’ section reports the experimentally proven effects of inducers and repressors (usually chemical compounds or environmental factors) on the level of protein (or mRNA) expression (up-regulation, down-regulation, constitutive expression).<p><a href='/help/induction' target='_top'>More...</a></p>Inductioni

Strongly repressed by glucose. Anaerobic growth of the pfl mutant in the presence of cAMP and L-threonine induced synthesis of TdcE.1 Publication

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGrid)

More...
BioGridi
4262415, 15 interactors

Database of interacting proteins

More...
DIPi
DIP-10972N

Protein interaction database and analysis system

More...
IntActi
P42632, 2 interactors

STRING: functional protein association networks

More...
STRINGi
511145.b3114

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
P42632

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family_and_domains_section">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini7 – 629PFLPROSITE-ProRule annotationAdd BLAST623
Domaini636 – 764Glycine radicalPROSITE-ProRule annotationAdd BLAST129

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
ENOG4105C6N Bacteria
COG1882 LUCA

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
HOG000011346

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
P42632

KEGG Orthology (KO)

More...
KOi
K00656

Database for complete collections of gene phylogenies

More...
PhylomeDBi
P42632

Family and domain databases

Conserved Domains Database

More...
CDDi
cd01678 PFL1, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR005949 Form_AcTrfase
IPR019777 Form_AcTrfase_GR_CS
IPR001150 Gly_radical
IPR004184 PFL_dom

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF01228 Gly_radical, 1 hit
PF02901 PFL-like, 1 hit

PIRSF; a whole-protein classification database

More...
PIRSFi
PIRSF000379 For_Ac_trans_1, 1 hit

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR01255 pyr_form_ly_1, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS00850 GLY_RADICAL_1, 1 hit
PS51149 GLY_RADICAL_2, 1 hit
PS51554 PFL, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P42632-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKVDIDTSDK LYADAWLGFK GTDWKNEINV RDFIQHNYTP YEGDESFLAE
60 70 80 90 100
ATPATTELWE KVMEGIRIEN ATHAPVDFDT NIATTITAHD AGYINQPLEK
110 120 130 140 150
IVGLQTDAPL KRALHPFGGI NMIKSSFHAY GREMDSEFEY LFTDLRKTHN
160 170 180 190 200
QGVFDVYSPD MLRCRKSGVL TGLPDGYGRG RIIGDYRRVA LYGISYLVRE
210 220 230 240 250
RELQFADLQS RLEKGEDLEA TIRLREELAE HRHALLQIQE MAAKYGFDIS
260 270 280 290 300
RPAQNAQEAV QWLYFAYLAA VKSQNGGAMS LGRTASFLDI YIERDFKAGV
310 320 330 340 350
LNEQQAQELI DHFIMKIRMV RFLRTPEFDS LFSGDPIWAT EVIGGMGLDG
360 370 380 390 400
RTLVTKNSFR YLHTLHTMGP APEPNLTILW SEELPIAFKK YAAQVSIVTS
410 420 430 440 450
SLQYENDDLM RTDFNSDDYA IACCVSPMVI GKQMQFFGAR ANLAKTLLYA
460 470 480 490 500
INGGVDEKLK IQVGPKTAPL MDDVLDYDKV MDSLDHFMDW LAVQYISALN
510 520 530 540 550
IIHYMHDKYS YEASLMALHD RDVYRTMACG IAGLSVATDS LSAIKYARVK
560 570 580 590 600
PIRDENGLAV DFEIDGEYPQ YGNNDERVDS IACDLVERFM KKIKALPTYR
610 620 630 640 650
NAVPTQSILT ITSNVVYGQK TGNTPDGRRA GTPFAPGANP MHGRDRKGAV
660 670 680 690 700
ASLTSVAKLP FTYAKDGISY TFSIVPAALG KEDPVRKTNL VGLLDGYFHH
710 720 730 740 750
EADVEGGQHL NVNVMNREML LDAIEHPEKY PNLTIRVSGY AVRFNALTRE
760
QQQDVISRTF TQAL
Length:764
Mass (Da):85,936
Last modified:October 11, 2004 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i2C4F46944118449B
GO

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

The sequence AAA57918 differs from that shown. Reason: Frameshift at position 742.Curated

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
U18997 Genomic DNA Translation: AAA57918.1 Frameshift.
U00096 Genomic DNA Translation: AAT48170.1
AP009048 Genomic DNA Translation: BAE77162.1

Protein sequence database of the Protein Information Resource

More...
PIRi
G65100

NCBI Reference Sequences

More...
RefSeqi
WP_000861734.1, NZ_LN832404.1
YP_026205.1, NC_000913.3

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...
EnsemblBacteriai
AAT48170; AAT48170; b3114
BAE77162; BAE77162; BAE77162

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
947623

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
ecj:JW5522
eco:b3114

Pathosystems Resource Integration Center (PATRIC)

More...
PATRICi
fig|1411691.4.peg.3616

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA Translation: AAA57918.1 Frameshift.
U00096 Genomic DNA Translation: AAT48170.1
AP009048 Genomic DNA Translation: BAE77162.1
PIRiG65100
RefSeqiWP_000861734.1, NZ_LN832404.1
YP_026205.1, NC_000913.3

3D structure databases

SMRiP42632
ModBaseiSearch...

Protein-protein interaction databases

BioGridi4262415, 15 interactors
DIPiDIP-10972N
IntActiP42632, 2 interactors
STRINGi511145.b3114

Proteomic databases

jPOSTiP42632
PaxDbiP42632
PRIDEiP42632

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAT48170; AAT48170; b3114
BAE77162; BAE77162; BAE77162
GeneIDi947623
KEGGiecj:JW5522
eco:b3114
PATRICifig|1411691.4.peg.3616

Organism-specific databases

EchoBASE - an integrated post-genomic database for E. coli

More...
EchoBASEi
EB2612
EcoGeneiEG12758 tdcE

Phylogenomic databases

eggNOGiENOG4105C6N Bacteria
COG1882 LUCA
HOGENOMiHOG000011346
InParanoidiP42632
KOiK00656
PhylomeDBiP42632

Enzyme and pathway databases

UniPathwayiUPA00052;UER00508
BioCyciEcoCyc:KETOBUTFORMLY-INACT-MONOMER
ECOL316407:JW5522-MONOMER
MetaCyc:KETOBUTFORMLY-INACT-MONOMER

Miscellaneous databases

Protein Ontology

More...
PROi
PR:P42632

Family and domain databases

CDDicd01678 PFL1, 1 hit
InterProiView protein in InterPro
IPR005949 Form_AcTrfase
IPR019777 Form_AcTrfase_GR_CS
IPR001150 Gly_radical
IPR004184 PFL_dom
PfamiView protein in Pfam
PF01228 Gly_radical, 1 hit
PF02901 PFL-like, 1 hit
PIRSFiPIRSF000379 For_Ac_trans_1, 1 hit
TIGRFAMsiTIGR01255 pyr_form_ly_1, 1 hit
PROSITEiView protein in PROSITE
PS00850 GLY_RADICAL_1, 1 hit
PS51149 GLY_RADICAL_2, 1 hit
PS51554 PFL, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiTDCE_ECOLI
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P42632
Secondary accession number(s): Q2M994, Q6BF44
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: October 11, 2004
Last modified: June 5, 2019
This is version 138 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
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