UniProtKB - P42574 (CASP3_HUMAN)
Protein
Caspase-3
Gene
CASP3
Organism
Homo sapiens (Human)
Status
Functioni
Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.2 Publications
Catalytic activityi
- Strict requirement for an Asp residue at positions P1 and P4. It has a preferred cleavage sequence of Asp-Xaa-Xaa-Asp-|- with a hydrophobic amino-acid residue at P2 and a hydrophilic amino-acid residue at P3, although Val or Ala are also accepted at this position. EC:3.4.22.56
Activity regulationi
Inhibited by isatin sulfonamides.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 121 | By similarity | 1 | |
Active sitei | 163 | By similarity | 1 |
GO - Molecular functioni
- aspartic-type endopeptidase activity Source: Ensembl
- cyclin-dependent protein serine/threonine kinase inhibitor activity Source: Ensembl
- cysteine-type endopeptidase activity Source: UniProtKB
- cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB
- cysteine-type endopeptidase activity involved in apoptotic signaling pathway Source: UniProtKB
- cysteine-type endopeptidase activity involved in execution phase of apoptosis Source: UniProtKB
- death receptor binding Source: Ensembl
- peptidase activity Source: UniProtKB
- phospholipase A2 activator activity Source: Ensembl
- protease binding Source: Ensembl
- protein-containing complex binding Source: Ensembl
GO - Biological processi
- anterior neural tube closure Source: Ensembl
- apoptotic DNA fragmentation Source: Reactome
- apoptotic process Source: UniProtKB
- apoptotic signaling pathway Source: BHF-UCL
- axonal fasciculation Source: Ensembl
- B cell homeostasis Source: Ensembl
- cell fate commitment Source: Ensembl
- cellular response to DNA damage stimulus Source: Ensembl
- cellular response to staurosporine Source: CAFA
- cytokine-mediated signaling pathway Source: Reactome
- erythrocyte differentiation Source: UniProtKB
- execution phase of apoptosis Source: UniProtKB
- extrinsic apoptotic signaling pathway in absence of ligand Source: Reactome
- glial cell apoptotic process Source: Ensembl
- heart development Source: Ensembl
- hippocampus development Source: Ensembl
- hippo signaling Source: Reactome
- intrinsic apoptotic signaling pathway in response to osmotic stress Source: Ensembl
- keratinocyte differentiation Source: GO_Central
- learning or memory Source: Ensembl
- leukocyte apoptotic process Source: Ensembl
- luteolysis Source: Ensembl
- negative regulation of activated T cell proliferation Source: Ensembl
- negative regulation of apoptotic process Source: MGI
- negative regulation of B cell proliferation Source: Ensembl
- neuron apoptotic process Source: Ensembl
- neuron differentiation Source: GO_Central
- neurotrophin TRK receptor signaling pathway Source: MGI
- platelet formation Source: UniProtKB
- positive regulation of amyloid-beta formation Source: UniProtKB
- positive regulation of apoptotic process Source: GO_Central
- positive regulation of neuron apoptotic process Source: Ensembl
- protein processing Source: Ensembl
- proteolysis Source: UniProtKB
- regulation of macroautophagy Source: ParkinsonsUK-UCL
- regulation of protein stability Source: UniProtKB
- response to amino acid Source: Ensembl
- response to antibiotic Source: Ensembl
- response to cobalt ion Source: Ensembl
- response to drug Source: Ensembl
- response to estradiol Source: Ensembl
- response to glucocorticoid Source: Ensembl
- response to glucose Source: Ensembl
- response to hydrogen peroxide Source: Ensembl
- response to hypoxia Source: Ensembl
- response to lipopolysaccharide Source: Ensembl
- response to nicotine Source: Ensembl
- response to tumor necrosis factor Source: BHF-UCL
- response to UV Source: Ensembl
- response to X-ray Source: Ensembl
- sensory perception of sound Source: Ensembl
- striated muscle cell differentiation Source: Ensembl
- T cell homeostasis Source: Ensembl
- wound healing Source: Ensembl
Keywordsi
Molecular function | Hydrolase, Protease, Thiol protease |
Biological process | Apoptosis |
Enzyme and pathway databases
BRENDAi | 3.4.22.56, 2681 |
PathwayCommonsi | P42574 |
Reactomei | R-HSA-111459, Activation of caspases through apoptosome-mediated cleavage R-HSA-111463, SMAC (DIABLO) binds to IAPs R-HSA-111464, SMAC(DIABLO)-mediated dissociation of IAP:caspase complexes R-HSA-111465, Apoptotic cleavage of cellular proteins R-HSA-111469, SMAC, XIAP-regulated apoptotic response R-HSA-140342, Apoptosis induced DNA fragmentation R-HSA-1474228, Degradation of the extracellular matrix R-HSA-2028269, Signaling by Hippo R-HSA-205025, NADE modulates death signalling R-HSA-211736, Stimulation of the cell death response by PAK-2p34 R-HSA-264870, Caspase-mediated cleavage of cytoskeletal proteins R-HSA-351906, Apoptotic cleavage of cell adhesion proteins R-HSA-418889, Caspase activation via Dependence Receptors in the absence of ligand R-HSA-449836, Other interleukin signaling |
SABIO-RKi | P42574 |
SIGNORi | P42574 |
Protein family/group databases
MEROPSi | C14.003 |
Names & Taxonomyi
Protein namesi | Recommended name: Caspase-3 (EC:3.4.22.56)Short name: CASP-3 Alternative name(s): Apopain Cysteine protease CPP32 Short name: CPP-32 Protein Yama SREBP cleavage activity 1 Short name: SCA-1 Cleaved into the following 2 chains: |
Gene namesi | Name:CASP3 Synonyms:CPP32 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:1504, CASP3 |
MIMi | 600636, gene |
neXtProti | NX_P42574 |
VEuPathDBi | HostDB:ENSG00000164305.17 |
Subcellular locationi
Other locations
Cytosol
- cytosol Source: UniProtKB
Nucleus
- nucleoplasm Source: Reactome
- nucleus Source: UniProtKB
Plasma Membrane
- death-inducing signaling complex Source: Ensembl
Other locations
- cytoplasm Source: GO_Central
- membrane raft Source: Ensembl
- neuronal cell body Source: Ensembl
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Organism-specific databases
DisGeNETi | 836 |
OpenTargetsi | ENSG00000164305 |
PharmGKBi | PA26087 |
Miscellaneous databases
Pharosi | P42574, Tchem |
Chemistry databases
ChEMBLi | CHEMBL2334 |
DrugBanki | DB08498, (1S)-1-(3-chlorophenyl)-2-oxo-2-[(1,3,4-trioxo-1,2,3,4-tetrahydroisoquinolin-5-yl)amino]ethyl acetate DB08497, (1S)-2-oxo-1-phenyl-2-[(1,3,4-trioxo-1,2,3,4-tetrahydroisoquinolin-5-yl)amino]ethyl acetate DB08213, 1-METHYL-5-(2-PHENOXYMETHYL-PYRROLIDINE-1-SULFONYL)-1H-INDOLE-2,3-DIONE DB06862, 2-HYDROXY-5-(2-MERCAPTO-ETHYLSULFAMOYL)-BENZOIC ACID DB08251, 4-[5-(2-CARBOXY-1-FORMYL-ETHYLCARBAMOYL)-PYRIDIN-3-YL]-BENZOIC ACID DB03124, 5-[4-(1-Carboxymethyl-2-Oxo-Propylcarbamoyl)-Benzylsulfamoyl]-2-Hydroxy-Benzoic Acid DB08229, [N-(3-dibenzylcarbamoyl-oxiranecarbonyl)-hydrazino]-acetic acid DB00945, Acetylsalicylic acid DB05408, Emricasan DB13751, Glycyrrhizic acid DB06255, Incadronic acid DB07696, methyl (3S)-3-[(tert-butoxycarbonyl)amino]-4-oxopentanoate DB01017, Minocycline DB08499, N-[3-(2-fluoroethoxy)phenyl]-N'-(1,3,4-trioxo-1,2,3,4-tetrahydroisoquinolin-6-yl)butanediamide DB00282, Pamidronic acid |
DrugCentrali | P42574 |
GuidetoPHARMACOLOGYi | 1619 |
Genetic variation databases
BioMutai | CASP3 |
DMDMi | 77416852 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
PropeptideiPRO_0000004569 | 1 – 9 | 9 | ||
PropeptideiPRO_0000004570 | 10 – 28 | 1 PublicationAdd BLAST | 19 | |
ChainiPRO_0000004571 | 29 – 175 | Caspase-3 subunit p17Add BLAST | 147 | |
ChainiPRO_0000004572 | 176 – 277 | Caspase-3 subunit p12Add BLAST | 102 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineCombined sources | 1 | |
Modified residuei | 11 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 26 | PhosphoserineCombined sources | 1 | |
Modified residuei | 163 | S-nitrosocysteine; in inhibited form1 Publication | 1 |
Post-translational modificationi
Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.
S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol.1 Publication
Keywords - PTMi
Acetylation, Phosphoprotein, S-nitrosylation, ZymogenProteomic databases
EPDi | P42574 |
jPOSTi | P42574 |
MassIVEi | P42574 |
MaxQBi | P42574 |
PaxDbi | P42574 |
PeptideAtlasi | P42574 |
PRIDEi | P42574 |
ProteomicsDBi | 55518 |
2D gel databases
OGPi | P42574 |
PTM databases
iPTMneti | P42574 |
MetOSitei | P42574 |
PhosphoSitePlusi | P42574 |
Expressioni
Tissue specificityi
Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
Gene expression databases
Bgeei | ENSG00000164305, Expressed in duodenum and 209 other tissues |
ExpressionAtlasi | P42574, baseline and differential |
Genevisiblei | P42574, HS |
Organism-specific databases
HPAi | ENSG00000164305, Tissue enhanced (blood) |
Interactioni
Subunit structurei
Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit.
Interacts with BIRC6/bruce.
1 PublicationBinary interactionsi
Hide detailsP42574
GO - Molecular functioni
- death receptor binding Source: Ensembl
- protease binding Source: Ensembl
Protein-protein interaction databases
BioGRIDi | 107286, 110 interactors |
ComplexPortali | CPX-970, Caspase-3 complex |
DIPi | DIP-268N |
ELMi | P42574 |
IntActi | P42574, 64 interactors |
MINTi | P42574 |
STRINGi | 9606.ENSP00000311032 |
Chemistry databases
BindingDBi | P42574 |
Miscellaneous databases
RNActi | P42574, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P42574 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P42574 |
Family & Domainsi
Sequence similaritiesi
Belongs to the peptidase C14A family.Curated
Phylogenomic databases
eggNOGi | KOG3573, Eukaryota |
GeneTreei | ENSGT00940000153232 |
HOGENOMi | CLU_036904_2_0_1 |
InParanoidi | P42574 |
OMAi | VALILNH |
OrthoDBi | 984395at2759 |
PhylomeDBi | P42574 |
TreeFami | TF102023 |
Family and domain databases
CDDi | cd00032, CASc, 1 hit |
InterProi | View protein in InterPro IPR015471, Casp3/7 IPR029030, Caspase-like_dom_sf IPR033139, Caspase_cys_AS IPR016129, Caspase_his_AS IPR002398, Pept_C14 IPR002138, Pept_C14_p10 IPR001309, Pept_C14_p20 IPR015917, Pept_C14A |
PANTHERi | PTHR10454, PTHR10454, 1 hit PTHR10454:SF31, PTHR10454:SF31, 1 hit |
PRINTSi | PR00376, IL1BCENZYME |
SMARTi | View protein in SMART SM00115, CASc, 1 hit |
SUPFAMi | SSF52129, SSF52129, 1 hit |
PROSITEi | View protein in PROSITE PS01122, CASPASE_CYS, 1 hit PS01121, CASPASE_HIS, 1 hit PS50207, CASPASE_P10, 1 hit PS50208, CASPASE_P20, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
P42574-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MENTENSVDS KSIKNLEPKI IHGSESMDSG ISLDNSYKMD YPEMGLCIII
60 70 80 90 100
NNKNFHKSTG MTSRSGTDVD AANLRETFRN LKYEVRNKND LTREEIVELM
110 120 130 140 150
RDVSKEDHSK RSSFVCVLLS HGEEGIIFGT NGPVDLKKIT NFFRGDRCRS
160 170 180 190 200
LTGKPKLFII QACRGTELDC GIETDSGVDD DMACHKIPVE ADFLYAYSTA
210 220 230 240 250
PGYYSWRNSK DGSWFIQSLC AMLKQYADKL EFMHILTRVN RKVATEFESF
260 270
SFDATFHAKK QIPCIVSMLT KELYFYH
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketC9JXR7 | C9JXR7_HUMAN | Caspase-3 | CASP3 | 117 | Annotation score: | ||
A8MVM1 | A8MVM1_HUMAN | Caspase-3 | CASP3 | 182 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 31 – 36 | ISLDNS → MSWDTG in CAC88866 (PubMed:15003516).Curated | 6 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_048616 | 22 | H → R. Corresponds to variant dbSNP:rs35578277Ensembl. | 1 | |
Natural variantiVAR_001401 | 190 | E → D3 PublicationsCorresponds to variant dbSNP:rs1049210Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U13737 mRNA Translation: AAA65015.1 U13738 mRNA Translation: AAB60355.1 U26943 mRNA Translation: AAA74929.1 AJ413269 mRNA Translation: CAC88866.1 AK291337 mRNA Translation: BAF84026.1 AY219866 Genomic DNA Translation: AAO25654.1 CH471056 Genomic DNA Translation: EAX04673.1 CH471056 Genomic DNA Translation: EAX04674.1 CH471056 Genomic DNA Translation: EAX04675.1 BC016926 mRNA Translation: AAH16926.1 |
CCDSi | CCDS3836.1 |
PIRi | A55315 |
RefSeqi | NP_004337.2, NM_004346.3 NP_116786.1, NM_032991.2 XP_011530603.1, XM_011532301.1 |
Genome annotation databases
Ensembli | ENST00000308394; ENSP00000311032; ENSG00000164305 ENST00000523916; ENSP00000428929; ENSG00000164305 |
GeneIDi | 836 |
KEGGi | hsa:836 |
UCSCi | uc003iwh.3, human |
Similar proteinsi
Cross-referencesi
Web resourcesi
NIEHS-SNPs |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U13737 mRNA Translation: AAA65015.1 U13738 mRNA Translation: AAB60355.1 U26943 mRNA Translation: AAA74929.1 AJ413269 mRNA Translation: CAC88866.1 AK291337 mRNA Translation: BAF84026.1 AY219866 Genomic DNA Translation: AAO25654.1 CH471056 Genomic DNA Translation: EAX04673.1 CH471056 Genomic DNA Translation: EAX04674.1 CH471056 Genomic DNA Translation: EAX04675.1 BC016926 mRNA Translation: AAH16926.1 |
CCDSi | CCDS3836.1 |
PIRi | A55315 |
RefSeqi | NP_004337.2, NM_004346.3 NP_116786.1, NM_032991.2 XP_011530603.1, XM_011532301.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1CP3 | X-ray | 2.30 | A/B | 1-277 | [»] | |
1GFW | X-ray | 2.80 | A | 29-175 | [»] | |
B | 181-277 | [»] | ||||
1I3O | X-ray | 2.70 | A/C | 1-175 | [»] | |
B/D | 176-277 | [»] | ||||
1NME | X-ray | 1.60 | A | 29-174 | [»] | |
B | 186-277 | [»] | ||||
1NMQ | X-ray | 2.40 | A/B | 29-277 | [»] | |
1NMS | X-ray | 1.70 | A/B | 29-277 | [»] | |
1PAU | X-ray | 2.50 | A | 29-175 | [»] | |
B | 176-277 | [»] | ||||
1QX3 | X-ray | 1.90 | A | 29-277 | [»] | |
1RE1 | X-ray | 2.50 | A | 29-175 | [»] | |
B | 176-277 | [»] | ||||
1RHJ | X-ray | 2.20 | A/C | 29-175 | [»] | |
B/D | 176-277 | [»] | ||||
1RHK | X-ray | 2.50 | A | 29-175 | [»] | |
B | 176-277 | [»] | ||||
1RHM | X-ray | 2.50 | A/C | 29-175 | [»] | |
B/D | 176-277 | [»] | ||||
1RHQ | X-ray | 3.00 | A/D | 29-175 | [»] | |
B/E | 176-277 | [»] | ||||
1RHR | X-ray | 3.00 | A | 29-175 | [»] | |
B | 176-277 | [»] | ||||
1RHU | X-ray | 2.51 | A | 29-175 | [»] | |
B | 176-277 | [»] | ||||
2C1E | X-ray | 1.77 | A | 29-175 | [»] | |
B | 176-277 | [»] | ||||
2C2K | X-ray | 1.87 | A | 29-175 | [»] | |
B | 176-277 | [»] | ||||
2C2M | X-ray | 1.94 | A | 29-175 | [»] | |
B | 176-277 | [»] | ||||
2C2O | X-ray | 2.45 | A | 29-175 | [»] | |
B | 176-277 | [»] | ||||
2CDR | X-ray | 1.70 | A | 29-175 | [»] | |
B | 176-277 | [»] | ||||
2CJX | X-ray | 1.70 | A | 29-175 | [»] | |
B | 176-277 | [»] | ||||
2CJY | X-ray | 1.67 | A | 29-175 | [»] | |
B | 176-277 | [»] | ||||
2CNK | X-ray | 1.75 | A | 29-175 | [»] | |
B | 176-277 | [»] | ||||
2CNL | X-ray | 1.67 | A | 29-175 | [»] | |
B | 176-277 | [»] | ||||
2CNN | X-ray | 1.70 | A | 29-175 | [»] | |
B | 176-277 | [»] | ||||
2CNO | X-ray | 1.95 | A | 29-175 | [»] | |
B | 176-277 | [»] | ||||
2DKO | X-ray | 1.06 | A | 29-174 | [»] | |
B | 175-277 | [»] | ||||
2H5I | X-ray | 1.69 | A | 29-174 | [»] | |
B | 184-277 | [»] | ||||
2H5J | X-ray | 2.00 | A/C | 29-174 | [»] | |
B/D | 184-277 | [»] | ||||
2H65 | X-ray | 2.30 | A/C | 29-174 | [»] | |
B/D | 184-277 | [»] | ||||
2J30 | X-ray | 1.40 | A | 29-277 | [»] | |
2J31 | X-ray | 1.50 | A | 29-277 | [»] | |
2J32 | X-ray | 1.30 | A | 29-277 | [»] | |
2J33 | X-ray | 2.00 | A | 29-277 | [»] | |
2XYG | X-ray | 1.54 | A | 29-174 | [»] | |
B | 185-277 | [»] | ||||
2XYH | X-ray | 1.89 | A | 29-174 | [»] | |
B | 185-277 | [»] | ||||
2XYP | X-ray | 1.86 | A | 29-174 | [»] | |
B | 185-277 | [»] | ||||
2XZD | X-ray | 2.10 | A/C | 27-175 | [»] | |
B/D | 176-277 | [»] | ||||
2XZT | X-ray | 2.70 | A/C | 29-175 | [»] | |
B/D | 176-277 | [»] | ||||
2Y0B | X-ray | 2.10 | A/C | 29-175 | [»] | |
B/D | 176-277 | [»] | ||||
3DEH | X-ray | 2.50 | A/B/C/D | 29-277 | [»] | |
3DEI | X-ray | 2.80 | A/B/C/D | 29-277 | [»] | |
3DEJ | X-ray | 2.60 | A/B/C/D | 29-277 | [»] | |
3DEK | X-ray | 2.40 | A/B/C/D | 29-277 | [»] | |
3EDQ | X-ray | 1.61 | A/C | 29-175 | [»] | |
B/D | 176-277 | [»] | ||||
3GJQ | X-ray | 2.60 | A/C | 29-175 | [»] | |
B/D | 176-277 | [»] | ||||
3GJR | X-ray | 2.20 | A/C | 29-175 | [»] | |
B/D | 176-277 | [»] | ||||
3GJS | X-ray | 1.90 | A/C | 29-175 | [»] | |
B/D | 176-277 | [»] | ||||
3GJT | X-ray | 2.20 | A/C | 29-175 | [»] | |
B/D | 176-277 | [»] | ||||
3H0E | X-ray | 2.00 | A/B | 29-277 | [»] | |
3ITN | X-ray | 1.63 | A | 29-277 | [»] | |
3KJF | X-ray | 2.00 | A | 29-175 | [»] | |
B | 176-277 | [»] | ||||
3PCX | X-ray | 1.50 | A | 29-277 | [»] | |
3PD0 | X-ray | 2.00 | A | 29-277 | [»] | |
3PD1 | X-ray | 1.62 | A | 29-277 | [»] | |
4DCJ | X-ray | 1.70 | A/D | 29-175 | [»] | |
B/E | 176-277 | [»] | ||||
4DCO | X-ray | 1.70 | A/D | 29-175 | [»] | |
B/E | 176-277 | [»] | ||||
4DCP | X-ray | 1.70 | A/D | 29-175 | [»] | |
B/E | 176-277 | [»] | ||||
4EHA | X-ray | 1.70 | A/C | 1-277 | [»] | |
4EHD | X-ray | 1.58 | A | 1-277 | [»] | |
4EHF | X-ray | 1.66 | A | 1-277 | [»] | |
4EHH | X-ray | 1.78 | A | 1-277 | [»] | |
4EHK | X-ray | 1.67 | A/C | 1-277 | [»] | |
4EHL | X-ray | 1.80 | A/C | 1-277 | [»] | |
4EHN | X-ray | 1.69 | A | 1-277 | [»] | |
4JJE | X-ray | 1.48 | A | 29-277 | [»] | |
4JQY | X-ray | 2.50 | A/B | 34-277 | [»] | |
4JQZ | X-ray | 2.89 | A/B | 34-277 | [»] | |
4JR0 | X-ray | 1.80 | A/B | 34-277 | [»] | |
4PRY | X-ray | 1.70 | A | 1-277 | [»] | |
4PS0 | X-ray | 1.63 | A/B | 1-277 | [»] | |
4QTX | X-ray | 1.97 | A | 1-277 | [»] | |
4QTY | X-ray | 1.60 | A | 29-277 | [»] | |
4QU0 | X-ray | 1.95 | A | 1-277 | [»] | |
4QU5 | X-ray | 1.91 | A | 1-277 | [»] | |
4QU8 | X-ray | 1.72 | A | 1-277 | [»] | |
4QU9 | X-ray | 1.56 | A | 1-277 | [»] | |
4QUA | X-ray | 1.89 | A | 1-277 | [»] | |
4QUB | X-ray | 1.69 | A | 1-277 | [»] | |
4QUD | X-ray | 2.00 | A/B | 1-277 | [»] | |
4QUE | X-ray | 1.84 | A/C | 1-277 | [»] | |
4QUG | X-ray | 1.92 | A/C | 1-277 | [»] | |
4QUH | X-ray | 1.76 | A/C | 1-277 | [»] | |
4QUI | X-ray | 1.76 | A/B | 1-277 | [»] | |
4QUJ | X-ray | 1.50 | A | 1-277 | [»] | |
4QUL | X-ray | 1.90 | A/C | 1-277 | [»] | |
5I9B | X-ray | 1.80 | A | 1-277 | [»] | |
5I9T | X-ray | 1.95 | A/C | 1-277 | [»] | |
5IAB | X-ray | 1.79 | A/C | 1-277 | [»] | |
5IAE | X-ray | 1.55 | A/C | 1-277 | [»] | |
5IAG | X-ray | 1.98 | A | 1-277 | [»] | |
5IAJ | X-ray | 1.58 | A | 1-277 | [»] | |
5IAK | X-ray | 1.82 | A | 1-277 | [»] | |
5IAN | X-ray | 2.70 | A | 1-277 | [»] | |
5IAR | X-ray | 1.76 | A | 1-277 | [»] | |
5IAS | X-ray | 1.54 | A | 1-277 | [»] | |
5IBC | X-ray | 1.66 | A | 1-277 | [»] | |
5IBP | X-ray | 1.38 | A | 1-277 | [»] | |
5IBR | X-ray | 1.74 | A/C | 1-277 | [»] | |
5IC4 | X-ray | 2.65 | A/C/E/G | 1-175 | [»] | |
B/D/F/H | 176-276 | [»] | ||||
SMRi | P42574 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 107286, 110 interactors |
ComplexPortali | CPX-970, Caspase-3 complex |
DIPi | DIP-268N |
ELMi | P42574 |
IntActi | P42574, 64 interactors |
MINTi | P42574 |
STRINGi | 9606.ENSP00000311032 |
Chemistry databases
BindingDBi | P42574 |
ChEMBLi | CHEMBL2334 |
DrugBanki | DB08498, (1S)-1-(3-chlorophenyl)-2-oxo-2-[(1,3,4-trioxo-1,2,3,4-tetrahydroisoquinolin-5-yl)amino]ethyl acetate DB08497, (1S)-2-oxo-1-phenyl-2-[(1,3,4-trioxo-1,2,3,4-tetrahydroisoquinolin-5-yl)amino]ethyl acetate DB08213, 1-METHYL-5-(2-PHENOXYMETHYL-PYRROLIDINE-1-SULFONYL)-1H-INDOLE-2,3-DIONE DB06862, 2-HYDROXY-5-(2-MERCAPTO-ETHYLSULFAMOYL)-BENZOIC ACID DB08251, 4-[5-(2-CARBOXY-1-FORMYL-ETHYLCARBAMOYL)-PYRIDIN-3-YL]-BENZOIC ACID DB03124, 5-[4-(1-Carboxymethyl-2-Oxo-Propylcarbamoyl)-Benzylsulfamoyl]-2-Hydroxy-Benzoic Acid DB08229, [N-(3-dibenzylcarbamoyl-oxiranecarbonyl)-hydrazino]-acetic acid DB00945, Acetylsalicylic acid DB05408, Emricasan DB13751, Glycyrrhizic acid DB06255, Incadronic acid DB07696, methyl (3S)-3-[(tert-butoxycarbonyl)amino]-4-oxopentanoate DB01017, Minocycline DB08499, N-[3-(2-fluoroethoxy)phenyl]-N'-(1,3,4-trioxo-1,2,3,4-tetrahydroisoquinolin-6-yl)butanediamide DB00282, Pamidronic acid |
DrugCentrali | P42574 |
GuidetoPHARMACOLOGYi | 1619 |
Protein family/group databases
MEROPSi | C14.003 |
PTM databases
iPTMneti | P42574 |
MetOSitei | P42574 |
PhosphoSitePlusi | P42574 |
Genetic variation databases
BioMutai | CASP3 |
DMDMi | 77416852 |
2D gel databases
OGPi | P42574 |
Proteomic databases
EPDi | P42574 |
jPOSTi | P42574 |
MassIVEi | P42574 |
MaxQBi | P42574 |
PaxDbi | P42574 |
PeptideAtlasi | P42574 |
PRIDEi | P42574 |
ProteomicsDBi | 55518 |
Protocols and materials databases
ABCDi | P42574, 3 sequenced antibodies |
Antibodypediai | 1222, 2350 antibodies |
CPTCi | P42574, 1 antibody |
DNASUi | 836 |
Genome annotation databases
Ensembli | ENST00000308394; ENSP00000311032; ENSG00000164305 ENST00000523916; ENSP00000428929; ENSG00000164305 |
GeneIDi | 836 |
KEGGi | hsa:836 |
UCSCi | uc003iwh.3, human |
Organism-specific databases
CTDi | 836 |
DisGeNETi | 836 |
GeneCardsi | CASP3 |
HGNCi | HGNC:1504, CASP3 |
HPAi | ENSG00000164305, Tissue enhanced (blood) |
MIMi | 600636, gene |
neXtProti | NX_P42574 |
OpenTargetsi | ENSG00000164305 |
PharmGKBi | PA26087 |
VEuPathDBi | HostDB:ENSG00000164305.17 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3573, Eukaryota |
GeneTreei | ENSGT00940000153232 |
HOGENOMi | CLU_036904_2_0_1 |
InParanoidi | P42574 |
OMAi | VALILNH |
OrthoDBi | 984395at2759 |
PhylomeDBi | P42574 |
TreeFami | TF102023 |
Enzyme and pathway databases
BRENDAi | 3.4.22.56, 2681 |
PathwayCommonsi | P42574 |
Reactomei | R-HSA-111459, Activation of caspases through apoptosome-mediated cleavage R-HSA-111463, SMAC (DIABLO) binds to IAPs R-HSA-111464, SMAC(DIABLO)-mediated dissociation of IAP:caspase complexes R-HSA-111465, Apoptotic cleavage of cellular proteins R-HSA-111469, SMAC, XIAP-regulated apoptotic response R-HSA-140342, Apoptosis induced DNA fragmentation R-HSA-1474228, Degradation of the extracellular matrix R-HSA-2028269, Signaling by Hippo R-HSA-205025, NADE modulates death signalling R-HSA-211736, Stimulation of the cell death response by PAK-2p34 R-HSA-264870, Caspase-mediated cleavage of cytoskeletal proteins R-HSA-351906, Apoptotic cleavage of cell adhesion proteins R-HSA-418889, Caspase activation via Dependence Receptors in the absence of ligand R-HSA-449836, Other interleukin signaling |
SABIO-RKi | P42574 |
SIGNORi | P42574 |
Miscellaneous databases
BioGRID-ORCSi | 836, 12 hits in 876 CRISPR screens |
ChiTaRSi | CASP3, human |
EvolutionaryTracei | P42574 |
GeneWikii | Caspase_3 |
GenomeRNAii | 836 |
Pharosi | P42574, Tchem |
PROi | PR:P42574 |
RNActi | P42574, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000164305, Expressed in duodenum and 209 other tissues |
ExpressionAtlasi | P42574, baseline and differential |
Genevisiblei | P42574, HS |
Family and domain databases
CDDi | cd00032, CASc, 1 hit |
InterProi | View protein in InterPro IPR015471, Casp3/7 IPR029030, Caspase-like_dom_sf IPR033139, Caspase_cys_AS IPR016129, Caspase_his_AS IPR002398, Pept_C14 IPR002138, Pept_C14_p10 IPR001309, Pept_C14_p20 IPR015917, Pept_C14A |
PANTHERi | PTHR10454, PTHR10454, 1 hit PTHR10454:SF31, PTHR10454:SF31, 1 hit |
PRINTSi | PR00376, IL1BCENZYME |
SMARTi | View protein in SMART SM00115, CASc, 1 hit |
SUPFAMi | SSF52129, SSF52129, 1 hit |
PROSITEi | View protein in PROSITE PS01122, CASPASE_CYS, 1 hit PS01121, CASPASE_HIS, 1 hit PS50207, CASPASE_P10, 1 hit PS50208, CASPASE_P20, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CASP3_HUMAN | |
Accessioni | P42574Primary (citable) accession number: P42574 Secondary accession number(s): A8K5M2 Q96KP2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | October 11, 2005 | |
Last modified: | February 10, 2021 | |
This is version 232 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Human chromosome 4
Human chromosome 4: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Peptidase families
Classification of peptidase families and list of entries