UniProtKB - P42566 (EPS15_HUMAN)

BLAST

>sp|P42566|EPS15_HUMAN Epidermal growth factor receptor substrate 15 OS=Homo sapiens OX=9606 GN=EPS15 PE=1 SV=2
MAAAAQLSLTQLSSGNPVYEKYYRQVDTGNTGRVLASDAAAFLKKSGLPDLILGKIWDLA
DTDGKGILNKQEFFVALRLVACAQNGLEVSLSSLNLAVPPPRFHDTSSPLLISGTSAAEL
PWAVKPEDKAKYDAIFDSLSPVNGFLSGDKVKPVLLNSKLPVDILGRVWELSDIDHDGML
DRDEFAVAMFLVYCALEKEPVPMSLPPALVPPSKRKTWVVSPAEKAKYDEIFLKTDKDMD
GFVSGLEVREIFLKTGLPSTLLAHIWSLCDTKDCGKLSKDQFALAFHLISQKLIKGIDPP
HVLTPEMIPPSDRASLQKNIIGSSPVADFSAIKELDTLNNEIVDLQREKNNVEQDLKEKE
DTIKQRTSEVQDLQDEVQRENTNLQKLQAQKQQVQELLDELDEQKAQLEEQLKEVRKKCA
EEAQLISSLKAELTSQESQISTYEEELAKAREELSRLQQETAELEESVESGKAQLEPLQQ
HLQDSQQEISSMQMKLMEMKDLENHNSQLNWCSSPHSILVNGATDYCSLSTSSSETANLN
EHVEGQSNLESEPIHQESPARSSPELLPSGVTDENEVTTAVTEKVCSELDNNRHSKEEDP
FNVDSSSLTGPVADTNLDFFQSDPFVGSDPFKDDPFGKIDPFGGDPFKGSDPFASDCFFR
QSTDPFATSSTDPFSAANNSSITSVETLKHNDPFAPGGTVVAASDSATDPFASVFGNESF
GGGFADFSTLSKVNNEDPFRSATSSSVSNVVITKNVFEETSVKSEDEPPALPPKIGTPTR
PCPLPPGKRSINKLDSPDPFKLNDPFQPFPGNDSPKEKDPEIFCDPFTSATTTTNKEADP
SNFANFSAYPSEEDMIEWAKRESEREEEQRLARLNQQEQEDLELAIALSKSEISEA

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History
Entry version 202 (07 Nov 2018)
Sequence version 2 (07 Jun 2005)
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Protein

Epidermal growth factor receptor substrate 15

Gene

EPS15

Organism

Homo sapiens (Human)

Status

Reviewed-Annotation score: -Experimental evidence at protein levelⁱ

Functionⁱ

Involved in cell growth regulation. May be involved in the regulation of mitogenic signals and control of cell proliferation. Involved in the internalization of ligand-inducible receptors of the receptor tyrosine kinase (RTK) type, in particular EGFR. Plays a role in the assembly of clathrin-coated pits (CCPs). Acts as a clathrin adapter required for post-Golgi trafficking. Seems to be involved in CCPs maturation including invagination or budding. Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR); internalization of ITGB1 as DAB2-dependent cargo but not TFR seems to require association with DAB2.4 Publications

Caution

Studies in clathrin-mediated endocytosis of ITGB1 and TFR used a siRNA mixture of EPS15 and EPS15L1, and a Dab2 mutant with impaired binding to EH domain-containing proteins EPS15 and ITSN1 suggesting a partially overlapping role of the EH domain-containing proteins.1 Publication

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Calcium bindingⁱ	173 – 184	1Add BLAST		12
Calcium bindingⁱ	236 – 247	2Add BLAST		12

GO - Molecular functionⁱ

cadherin binding
Source: BHF-UCL
Inferred from high throughput direct assayⁱ
- 25468996
calcium ion binding Source: InterPro
cargo receptor activity Source: Ensembl
identical protein binding Source: Ensembl
polyubiquitin modification-dependent protein binding
Source: UniProtKB
Inferred from direct assayⁱ
- 24768539
SH3 domain binding Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

cell proliferation
Source: ProtInc
Traceable author statementⁱ
- 8183552
clathrin coat assembly
Source: BHF-UCL
Inferred from direct assayⁱ
- 12807910
endocytic recycling
Source: BHF-UCL
Inferred by curatorⁱ
- 12807910
epidermal growth factor receptor signaling pathway
Source: ProtInc
Traceable author statementⁱ
- 8183552
Golgi to endosome transport
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 24768539
membrane organization Source: Reactome
negative regulation of epidermal growth factor receptor signaling pathway Source: Reactome
positive regulation of receptor recycling Source: Ensembl
postsynaptic neurotransmitter receptor internalization Source: Ensembl
protein transport Source: UniProtKB-KW
receptor-mediated endocytosis of virus by host cell
Source: CACAO
Inferred from mutant phenotypeⁱ
- 21047970
regulation of cell proliferation Source: Ensembl
vesicle organization
Source: UniProtKB
Traceable author statementⁱ
- 9762922
viral entry into host cell
Source: CACAO
Inferred from mutant phenotypeⁱ
- 20202662

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Biological process	Endocytosis, Host-virus interaction, Protein transport, Transport
Ligand	Calcium, Metal-binding

Enzyme and pathway databases

Reactomeⁱ	R-HSA-182971 EGFR downregulation R-HSA-6807004 Negative regulation of MET activity R-HSA-8856825 Cargo recognition for clathrin-mediated endocytosis R-HSA-8856828 Clathrin-mediated endocytosis R-HSA-8875360 InlB-mediated entry of Listeria monocytogenes into host cell
SignaLinkⁱ	P42566
SIGNORⁱ	P42566

Protein family/group databases

MoonDBⁱ	P42566 Curated
MoonProtⁱ	P42566

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Epidermal growth factor receptor substrate 15 Short name: Protein Eps15 Alternative name(s): Protein AF-1p
Gene namesⁱ	Name:EPS15 Synonyms:AF1P
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 1

Organism-specific databases

EuPathDBⁱ	HostDB:ENSG00000085832.16
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:3419 EPS15
MIMⁱ	600051 gene
neXtProtⁱ	NX_P42566

Keywords - Cellular componentⁱ

Cell membrane, Coated pit, Cytoplasm, Endosome, Membrane

Pathology & Biotechⁱ

Involvement in diseaseⁱ

A chromosomal aberration involving EPS15 is found in acute leukemias. Translocation t(1;11)(p32;q23) with KMT2A/MLL1. The result is a rogue activator protein.

Mutagenesis

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Mutagenesisⁱ	154	V → E: Loss of interaction with STON2 NPF motifs. 1 Publication		1
Mutagenesisⁱ	169	W → A: Loss of interaction with STON2 NPF motifs. 1 Publication		1

Keywords - Diseaseⁱ

Proto-oncogene

Organism-specific databases

DisGeNET More...DisGeNETⁱ	2060
Open Targets More...OpenTargetsⁱ	ENSG00000085832
PharmGKBⁱ	PA27838

Polymorphism and mutation databases

BioMutaⁱ	EPS15
DMDMⁱ	67476728

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Initiator methionineⁱ		RemovedCombined sources
ChainⁱPRO_0000146116	2 – 896	Epidermal growth factor receptor substrate 15Add BLAST		895

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	2	N-acetylalanineCombined sources	1
Modified residueⁱ	108	PhosphoserineCombined sources	1
Modified residueⁱ	140	PhosphoserineCombined sources	1
Modified residueⁱ	323	PhosphoserineCombined sources	1
Modified residueⁱ	324	PhosphoserineCombined sources	1
Modified residueⁱ	467	PhosphoserineCombined sources	1
Modified residueⁱ	470	PhosphoserineCombined sources	1
Modified residueⁱ	485	PhosphoserineCombined sources	1
Modified residueⁱ	562	PhosphoserineBy similarity	1
Modified residueⁱ	563	PhosphoserineBy similarity	1
Modified residueⁱ	746	PhosphoserineCombined sources	1
Modified residueⁱ	777	PhosphothreonineCombined sources	1
Modified residueⁱ	779	PhosphothreonineBy similarity	1
Modified residueⁱ	790	PhosphoserineCombined sources	1
Modified residueⁱ	796	PhosphoserineCombined sources	1
Modified residueⁱ	814	PhosphoserineCombined sources	1
Modified residueⁱ	849	Phosphotyrosine; by EGFRCombined sources	1

Post-translational modificationⁱ

Phosphorylation on Tyr-849 is involved in the internalization of EGFR. Not required for membrane translocation after EGF treatment or for targeting to coated pits, but essential for a subsequent step in EGFR endocytosis (By similarity). Phosphorylated on serine upon DNA damage, probably by ATM or ATR.By similarity

Ubiquitinated.By similarity

Keywords - PTMⁱ

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDⁱ	P42566
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	P42566
PaxDbⁱ	P42566
PeptideAtlas More...PeptideAtlasⁱ	P42566
PRIDEⁱ	P42566
ProteomicsDBⁱ	55515 55516 [P42566-2]

2D gel databases

USC-OGP 2-DE database More...OGPⁱ	P42566

OGPⁱ

P42566

PTM databases

iPTMnetⁱ	P42566
PhosphoSitePlusⁱ	P42566

Expressionⁱ

Tissue specificityⁱ

Ubiquitously expressed.

Gene expression databases

Bgeeⁱ	ENSG00000085832 Expressed in 241 organ(s), highest expression level in endothelial cell
CleanExⁱ	HS_EPS15
ExpressionAtlasⁱ	P42566 baseline and differential
Genevisibleⁱ	P42566 HS

Organism-specific databases

Human Protein Atlas More...HPAⁱ	CAB010164 HPA008451 HPA061431

HPAⁱ

CAB010164
HPA008451
HPA061431

Interactionⁱ

Subunit structureⁱ

Interacts with SGIP1 (PubMed:26822536). Interacts with HGS; the interaction bridges the interaction of STAM or STAM2 with EPS15. Isoform 2 interacts with HGS and AP2A2. Part of a complex at least composed of EPS15, HGS, and either STAM or STAM2. Binds AP2A2. Interacts with AP2B1; clathrin competes with EPS15. Binds STON2 and EPN1. Interacts (via its SH3-binding sites) with CRK. Interacts with SH3BP4/TTP. Interacts with ERBB2. Interacts with FCHO1. Interacts with FCHO2. Interacts (via EH domains) with DAB2. Interacts (via UIM repeats) with CORO7 (when ubiquitinated at 'Lys-472'). Interacts (via UIM domains) with UBQLN1 (via ubiquitin-like domain) and can interact with both the ubiquitinated and the non-ubiquitinated forms of UBQLN1. Interacts with UBQLN2 (By similarity) (PubMed:10757979, PubMed:11062555, PubMed:12551915, PubMed:16159959, PubMed:16314522, PubMed:16325581, PubMed:16903783, PubMed:18199683, PubMed:18200045, PubMed:18362181, PubMed:20448150, PubMed:21762413, PubMed:22484487, PubMed:22648170, PubMed:24768539, PubMed:8662907, PubMed:9721102, PubMed:9723620).By similarity19 Publications

(Microbial infection) Interacts with vaccinia virus protein A36.1 Publication

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
FCHO2	Q0JRZ9	3	EBI-396684,EBI-2609756
Fcho2	Q3UQN2	3	EBI-396684,EBI-6094986	From Mus musculus.
ITSN1	Q15811	2	EBI-396684,EBI-602041
NCK1	P16333	2	EBI-396684,EBI-389883
Numb	Q9QZS3-1	3	EBI-396684,EBI-9547433	From a different organism.
Numb	Q9QZS3-2	3	EBI-396684,EBI-3896014	From a different organism.
PTPN3	P26045	4	EBI-15895294,EBI-1047946
STON2	Q8WXE9	17	EBI-396684,EBI-539742
UBQLN1	Q9UMX0	5	EBI-396684,EBI-741480

GO - Molecular functionⁱ

cadherin binding
Source: BHF-UCL
Inferred from high throughput direct assayⁱ
- 25468996
identical protein binding Source: Ensembl
polyubiquitin modification-dependent protein binding
Source: UniProtKB
Inferred from direct assayⁱ
- 24768539
SH3 domain binding Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	108374, 164 interactors
CORUMⁱ	P42566
Database of interacting proteins More...DIPⁱ	DIP-33064N
ELMⁱ	P42566
IntActⁱ	P42566, 79 interactors
Molecular INTeraction database More...MINTⁱ	P42566
STRINGⁱ	9606.ENSP00000360798

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Helixⁱ	126 – 136	Combined sources	11
Beta strandⁱ	142 – 144	Combined sources	3
Beta strandⁱ	145 – 147	Combined sources	3
Helixⁱ	148 – 156	Combined sources	9
Turnⁱ	157 – 159	Combined sources	3
Helixⁱ	162 – 172	Combined sources	11
Beta strandⁱ	177 – 179	Combined sources	3
Helixⁱ	182 – 197	Combined sources	16
Turnⁱ	207 – 209	Combined sources	3
Helixⁱ	212 – 214	Combined sources	3
Helixⁱ	223 – 235	Combined sources	13
Beta strandⁱ	240 – 243	Combined sources	4
Helixⁱ	245 – 253	Combined sources	9
Turnⁱ	254 – 256	Combined sources	3
Helixⁱ	259 – 269	Combined sources	11
Beta strandⁱ	274 – 278	Combined sources	5
Turnⁱ	279 – 281	Combined sources	3
Helixⁱ	282 – 293	Combined sources	12
Turnⁱ	305 – 307	Combined sources	3
Turnⁱ	624 – 627	Combined sources	4
Turnⁱ	630 – 633	Combined sources	4
Helixⁱ	723 – 726	Combined sources	4

Show more details

3D structure databases

ProteinModelPortalⁱ	P42566
SMRⁱ	P42566
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ	P42566

EvolutionaryTraceⁱ

P42566

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Length
Domainⁱ	15 – 104	EH 1PROSITE-ProRule annotationAdd BLAST	90
Domainⁱ	128 – 216	EH 2PROSITE-ProRule annotationAdd BLAST	89
Domainⁱ	160 – 195	EF-hand 1PROSITE-ProRule annotationAdd BLAST	36
Domainⁱ	223 – 258	EF-hand 2PROSITE-ProRule annotationAdd BLAST	36
Domainⁱ	224 – 314	EH 3PROSITE-ProRule annotationAdd BLAST	91
Repeatⁱ	599 – 601	1	3
Repeatⁱ	623 – 625	2	3
Repeatⁱ	629 – 631	3	3
Repeatⁱ	634 – 636	4	3
Repeatⁱ	640 – 642	5	3
Repeatⁱ	645 – 647	6	3
Repeatⁱ	651 – 653	7	3
Repeatⁱ	664 – 666	8	3
Repeatⁱ	672 – 674	9	3
Repeatⁱ	692 – 694	10	3
Repeatⁱ	709 – 711	11	3
Repeatⁱ	737 – 739	12	3
Repeatⁱ	798 – 800	13	3
Repeatⁱ	804 – 806	14	3
Repeatⁱ	825 – 827	15	3
Domainⁱ	851 – 870	UIM 1PROSITE-ProRule annotationAdd BLAST	20
Domainⁱ	877 – 896	UIM 2PROSITE-ProRule annotationAdd BLAST	20

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	2 – 330	Interaction with DAB2By similarityAdd BLAST		329
Regionⁱ	599 – 827	15 X 3 AA repeats of D-P-FAdd BLAST		229

Motif

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Motifⁱ	768 – 774	SH3-binding		7

Compositional bias

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Compositional biasⁱ	768 – 850	Pro-richAdd BLAST		83

Domainⁱ

The EH domain interacts with Asn-Pro-Phe (NPF) motifs of target proteins.1 Publication

Keywords - Domainⁱ

Repeat, SH3-binding

Phylogenomic databases

eggNOGⁱ	KOG0998 Eukaryota ENOG410XTDR LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00760000118985
HOGENOMⁱ	HOG000004804
HOVERGENⁱ	HBG005591
InParanoidⁱ	P42566
KEGG Orthology (KO) More...KOⁱ	K12472
OMAⁱ	EKVCPEF
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G01RG
PhylomeDBⁱ	P42566
TreeFamⁱ	TF324293

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00052 EH, 3 hits
InterProⁱ	View protein in InterPro IPR011992 EF-hand-dom_pair IPR018247 EF_Hand_1_Ca_BS IPR002048 EF_hand_dom IPR000261 EH_dom IPR003903 UIM_dom
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF12763 EF-hand_4, 3 hits
SMARTⁱ	View protein in SMART SM00054 EFh, 3 hits SM00027 EH, 3 hits SM00726 UIM, 3 hits
SUPFAMⁱ	SSF47473 SSF47473, 3 hits
PROSITEⁱ	View protein in PROSITE PS00018 EF_HAND_1, 2 hits PS50222 EF_HAND_2, 4 hits PS50031 EH, 3 hits PS50330 UIM, 2 hits

Sequences (2+)ⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsⁱ produced by alternative splicing. Align Add to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show all Align All

Isoform 1 (identifier: P42566-1) [UniParc]FASTA Add to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAAAQLSLT QLSSGNPVYE KYYRQVDTGN TGRVLASDAA AFLKKSGLPD 
        60         70         80         90        100
LILGKIWDLA DTDGKGILNK QEFFVALRLV ACAQNGLEVS LSSLNLAVPP 
       110        120        130        140        150
PRFHDTSSPL LISGTSAAEL PWAVKPEDKA KYDAIFDSLS PVNGFLSGDK 
       160        170        180        190        200
VKPVLLNSKL PVDILGRVWE LSDIDHDGML DRDEFAVAMF LVYCALEKEP 
       210        220        230        240        250
VPMSLPPALV PPSKRKTWVV SPAEKAKYDE IFLKTDKDMD GFVSGLEVRE 
       260        270        280        290        300
IFLKTGLPST LLAHIWSLCD TKDCGKLSKD QFALAFHLIS QKLIKGIDPP 
       310        320        330        340        350
HVLTPEMIPP SDRASLQKNI IGSSPVADFS AIKELDTLNN EIVDLQREKN 
       360        370        380        390        400
NVEQDLKEKE DTIKQRTSEV QDLQDEVQRE NTNLQKLQAQ KQQVQELLDE 
       410        420        430        440        450
LDEQKAQLEE QLKEVRKKCA EEAQLISSLK AELTSQESQI STYEEELAKA 
       460        470        480        490        500
REELSRLQQE TAELEESVES GKAQLEPLQQ HLQDSQQEIS SMQMKLMEMK 
       510        520        530        540        550
DLENHNSQLN WCSSPHSILV NGATDYCSLS TSSSETANLN EHVEGQSNLE 
       560        570        580        590        600
SEPIHQESPA RSSPELLPSG VTDENEVTTA VTEKVCSELD NNRHSKEEDP 
       610        620        630        640        650
FNVDSSSLTG PVADTNLDFF QSDPFVGSDP FKDDPFGKID PFGGDPFKGS 
       660        670        680        690        700
DPFASDCFFR QSTDPFATSS TDPFSAANNS SITSVETLKH NDPFAPGGTV 
       710        720        730        740        750
VAASDSATDP FASVFGNESF GGGFADFSTL SKVNNEDPFR SATSSSVSNV 
       760        770        780        790        800
VITKNVFEET SVKSEDEPPA LPPKIGTPTR PCPLPPGKRS INKLDSPDPF 
       810        820        830        840        850
KLNDPFQPFP GNDSPKEKDP EIFCDPFTSA TTTTNKEADP SNFANFSAYP 
       860        870        880        890 
SEEDMIEWAK RESEREEEQR LARLNQQEQE DLELAIALSK SEISEA

Length:896

Mass (Da):98,656

Last modified:June 7, 2005 - v2

Checksum:ⁱA1B9FE15B47ABAFF

Isoform 2 (identifier: P42566-2) [UniParc]FASTA Add to basket

Also known as: Eps15b

The sequence of this isoform differs from the canonical sequence as follows:
1-314: Missing.
315-346: SLQKNIIGSSPVADFSAIKELDTLNNEIVDLQ → MYLKSDSGLGGWITIPAVADVLKYSCIVCWSS

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        10         20         30         40         50
MYLKSDSGLG GWITIPAVAD VLKYSCIVCW SSREKNNVEQ DLKEKEDTIK 
        60         70         80         90        100
QRTSEVQDLQ DEVQRENTNL QKLQAQKQQV QELLDELDEQ KAQLEEQLKE 
       110        120        130        140        150
VRKKCAEEAQ LISSLKAELT SQESQISTYE EELAKAREEL SRLQQETAEL 
       160        170        180        190        200
EESVESGKAQ LEPLQQHLQD SQQEISSMQM KLMEMKDLEN HNSQLNWCSS 
       210        220        230        240        250
PHSILVNGAT DYCSLSTSSS ETANLNEHVE GQSNLESEPI HQESPARSSP 
       260        270        280        290        300
ELLPSGVTDE NEVTTAVTEK VCSELDNNRH SKEEDPFNVD SSSLTGPVAD 
       310        320        330        340        350
TNLDFFQSDP FVGSDPFKDD PFGKIDPFGG DPFKGSDPFA SDCFFRQSTD 
       360        370        380        390        400
PFATSSTDPF SAANNSSITS VETLKHNDPF APGGTVVAAS DSATDPFASV 
       410        420        430        440        450
FGNESFGGGF ADFSTLSKVN NEDPFRSATS SSVSNVVITK NVFEETSVKS 
       460        470        480        490        500
EDEPPALPPK IGTPTRPCPL PPGKRSINKL DSPDPFKLND PFQPFPGNDS 
       510        520        530        540        550
PKEKDPEIFC DPFTSATTTT NKEADPSNFA NFSAYPSEED MIEWAKRESE 
       560        570        580 
REEEQRLARL NQQEQEDLEL AIALSKSEIS EA

Show »

Length:582

Mass (Da):64,376

Checksum:ⁱ2A7BACA24604E8CA

Computationally mapped potential isoform sequencesⁱ

There are 2 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

Entry	Entry name	Protein names	Gene names	Length	Annotation

B1AUU8	B1AUU8_HUMAN	Epidermal growth factor receptor su... Epidermal growth factor receptor substrate 15	EPS15	762	Annotation score:
S4R3U1	S4R3U1_HUMAN	Epidermal growth factor receptor su... Epidermal growth factor receptor substrate 15	EPS15	90	Annotation score:

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	446	Missing in BX647676 (PubMed:17974005).Curated		1

Natural variant

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Natural variantⁱVAR_016142	822	I → M1 PublicationCorresponds to variant dbSNP:rs17567Ensembl.		1

Alternative sequence

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Alternative sequenceⁱVSP_036168	1 – 314	Missing in isoform 2. 1 PublicationAdd BLAST		314
Alternative sequenceⁱVSP_036169	315 – 346	SLQKN…IVDLQ → MYLKSDSGLGGWITIPAVAD VLKYSCIVCWSS in isoform 2. 1 PublicationAdd BLAST		32

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U07707 mRNA Translation: AAA52101.1 Z29064 mRNA Translation: CAA82305.1 AK313396 mRNA Translation: BAG36194.1 DQ367924 mRNA Translation: ABD34786.1 BX647676 mRNA No translation available. AC104170 Genomic DNA No translation available. AL671986 Genomic DNA No translation available. CH471059 Genomic DNA Translation: EAX06823.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS557.1 [P42566-1]
PIRⁱ	S43074
NCBI Reference Sequences More...RefSeqⁱ	NP_001153441.1, NM_001159969.1 [P42566-2] NP_001972.1, NM_001981.2 [P42566-1]
UniGeneⁱ	Hs.83722

Genome annotation databases

Ensemblⁱ	ENST00000371733; ENSP00000360798; ENSG00000085832 [P42566-1]
GeneIDⁱ	2060
KEGGⁱ	hsa:2060
UCSC genome browser More...UCSCⁱ	uc001csq.2 human [P42566-1]

Keywords - Coding sequence diversityⁱ

Alternative splicing, Chromosomal rearrangement, Polymorphism

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
P42566	Uncharacterized protein		SHEEP		912	UniRef90_P42566
EPS15 protein		BOVIN		910
epidermal growth factor receptor substrate 15 isoform X1		Enhydra lutris kenyoni		902
Epidermal growth factor receptor pathway substrate 15 (Fragment)		NEOVI		353
epidermal growth factor receptor substrate 15		LIPVE		902
+128
P42566-2	Macaca fascicularis brain cDNA clone: QflA-19426, similar to human epidermal growth factor receptor pathway substrate 15(EPS15), mRNA, RefSeq: NM_001981.1		MACFA		583	UniRef90_P42566-2
Epidermal growth factor receptor substrate 15 isoform B		MACMU		583
Epidermal growth factor receptor pathway substrate 15		PANTR		582
cDNA FLJ55050, highly similar to Epidermal growth factor receptor substrate 15		HUMAN		573
UPI0008F499F3		GORGO		642
+7

Protein	Similar proteins		Species	Score	Length	Source
P42566	Uncharacterized protein		SHEEP		912	UniRef50_P42566
EPS15 protein		BOVIN		910
epidermal growth factor receptor substrate 15 isoform X1		Enhydra lutris kenyoni		902
Epidermal growth factor receptor pathway substrate 15 (Fragment)		NEOVI		353
epidermal growth factor receptor substrate 15		LIPVE		902
+129
P42566-2	Macaca fascicularis brain cDNA clone: QflA-19426, similar to human epidermal growth factor receptor pathway substrate 15(EPS15), mRNA, RefSeq: NM_001981.1		MACFA		583	UniRef50_P42566-2
Epidermal growth factor receptor substrate 15 isoform B		MACMU		583
Epidermal growth factor receptor pathway substrate 15		PANTR		582
cDNA FLJ55050, highly similar to Epidermal growth factor receptor substrate 15		HUMAN		573
epidermal growth factor receptor substrate 15 isoform X2		BALAS		589
+44

Cross-referencesⁱ

Web resourcesⁱ

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U07707 mRNA Translation: AAA52101.1 Z29064 mRNA Translation: CAA82305.1 AK313396 mRNA Translation: BAG36194.1 DQ367924 mRNA Translation: ABD34786.1 BX647676 mRNA No translation available. AC104170 Genomic DNA No translation available. AL671986 Genomic DNA No translation available. CH471059 Genomic DNA Translation: EAX06823.1
CCDSⁱ	CCDS557.1 [P42566-1]
PIRⁱ	S43074
RefSeqⁱ	NP_001153441.1, NM_001159969.1 [P42566-2] NP_001972.1, NM_001981.2 [P42566-1]
UniGeneⁱ	Hs.83722

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	1C07	NMR	-	A	217-311	[»]
	1EH2	NMR	-	A	121-218	[»]
	1F8H	NMR	-	A	121-215	[»]
	1FF1	NMR	-	A	121-215	[»]
	2IV9	X-ray	1.90	P	723-730	[»]
	2JXC	NMR	-	A	121-215	[»]
	4RH5	X-ray	1.60	B	846-854	[»]
	4RH9	X-ray	1.60	B	846-854	[»]
	4RHG	X-ray	1.58	B	846-854	[»]
	4S0G	X-ray	1.72	B	846-854	[»]
	5AWT	X-ray	2.70	B	640-649	[»]
	5AWU	X-ray	2.70	B	645-654	[»]
	5JP2	X-ray	2.40	E/F	615-637	[»]
ProteinModelPortalⁱ	P42566
SMRⁱ	P42566
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	108374, 164 interactors
CORUMⁱ	P42566
DIPⁱ	DIP-33064N
ELMⁱ	P42566
IntActⁱ	P42566, 79 interactors
MINTⁱ	P42566
STRINGⁱ	9606.ENSP00000360798

Protein family/group databases

MoonDBⁱ	P42566 Curated
MoonProtⁱ	P42566

PTM databases

iPTMnetⁱ	P42566
PhosphoSitePlusⁱ	P42566

Polymorphism and mutation databases

BioMutaⁱ	EPS15
DMDMⁱ	67476728

2D gel databases

OGPⁱ	P42566

OGPⁱ

P42566

Proteomic databases

EPDⁱ	P42566
MaxQBⁱ	P42566
PaxDbⁱ	P42566
PeptideAtlasⁱ	P42566
PRIDEⁱ	P42566
ProteomicsDBⁱ	55515 55516 [P42566-2]

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENST00000371733; ENSP00000360798; ENSG00000085832 [P42566-1]
GeneIDⁱ	2060
KEGGⁱ	hsa:2060
UCSCⁱ	uc001csq.2 human [P42566-1]

Organism-specific databases

CTDⁱ	2060
DisGeNETⁱ	2060
EuPathDBⁱ	HostDB:ENSG00000085832.16
GeneCardsⁱ	EPS15
HGNCⁱ	HGNC:3419 EPS15
HPAⁱ	CAB010164 HPA008451 HPA061431
MIMⁱ	600051 gene
neXtProtⁱ	NX_P42566
OpenTargetsⁱ	ENSG00000085832
PharmGKBⁱ	PA27838
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	KOG0998 Eukaryota ENOG410XTDR LUCA
GeneTreeⁱ	ENSGT00760000118985
HOGENOMⁱ	HOG000004804
HOVERGENⁱ	HBG005591
InParanoidⁱ	P42566
KOⁱ	K12472
OMAⁱ	EKVCPEF
OrthoDBⁱ	EOG091G01RG
PhylomeDBⁱ	P42566
TreeFamⁱ	TF324293

Enzyme and pathway databases

Reactomeⁱ	R-HSA-182971 EGFR downregulation R-HSA-6807004 Negative regulation of MET activity R-HSA-8856825 Cargo recognition for clathrin-mediated endocytosis R-HSA-8856828 Clathrin-mediated endocytosis R-HSA-8875360 InlB-mediated entry of Listeria monocytogenes into host cell
SignaLinkⁱ	P42566
SIGNORⁱ	P42566

Miscellaneous databases

ChiTaRSⁱ	EPS15 human
EvolutionaryTraceⁱ	P42566
GeneWikiⁱ	EPS15
GenomeRNAiⁱ	2060
Protein Ontology More...PROⁱ	PR:P42566
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSG00000085832 Expressed in 241 organ(s), highest expression level in endothelial cell
CleanExⁱ	HS_EPS15
ExpressionAtlasⁱ	P42566 baseline and differential
Genevisibleⁱ	P42566 HS

Family and domain databases

CDDⁱ	cd00052 EH, 3 hits
InterProⁱ	View protein in InterPro IPR011992 EF-hand-dom_pair IPR018247 EF_Hand_1_Ca_BS IPR002048 EF_hand_dom IPR000261 EH_dom IPR003903 UIM_dom
Pfamⁱ	View protein in Pfam PF12763 EF-hand_4, 3 hits
SMARTⁱ	View protein in SMART SM00054 EFh, 3 hits SM00027 EH, 3 hits SM00726 UIM, 3 hits
SUPFAMⁱ	SSF47473 SSF47473, 3 hits
PROSITEⁱ	View protein in PROSITE PS00018 EF_HAND_1, 2 hits PS50222 EF_HAND_2, 4 hits PS50031 EH, 3 hits PS50330 UIM, 2 hits
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	EPS15_HUMAN
Accessionⁱ	P42566Primary (citable) accession number: P42566 Secondary accession number(s): B2R8J7, D3DPJ2, Q5SRH4
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
	Last sequence update:	June 7, 2005
	Last modified:	November 7, 2018
	This is version 202 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

Documents

Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM
Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations
Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations
MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
PDB cross-references
Index of Protein Data Bank (PDB) cross-references

UniProtKB

UniParc

Help

UniRef

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Supporting data

UniProtKB - P42566 (EPS15_HUMAN)

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Epidermal growth factor receptor substrate 15

EPS15

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Caution

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

Protein family/group databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Plasma membrane

Other locations

Endosome

Cytosol

Endosome

Plasma Membrane

Other locations

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Keywords - Diseasei

Organism-specific databases

Polymorphism and mutation databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

2D gel databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Organism-specific databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Region

Motif

Compositional bias

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Experimental Info

Natural variant

Alternative sequence

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

<p>This subsection of the <a href="http://www.uniprot.org/manual/cross_references_section">Cross-references</a> section provides links to various web resources that are relevant for a specific protein.<p><a href='/help/web_resource' target='_top'>More...</a></p>Web resourcesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

PTM databases

Polymorphism and mutation databases

2D gel databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

Keywords - Diseaseⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Keywords - Domainⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Web resourcesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ