UniProtKB - P42494 (DPOLX_ASFB7)
Repair DNA polymerase X
Ba71V-97
Functioni
Error-prone polymerase lacking a proofreading 3'-5' exonuclease which catalyzes the gap-filling reaction during the DNA repair process (PubMed:11685239, PubMed:28245220).
Specifically binds intermediates in the single-nucleotide base-excision repair process (PubMed:12595253).
Also catalyzes DNA polymerization with low nucleotide-insertion fidelity (PubMed:24617852).
Probably acts as a strategic DNA mutase, which gives rise to a rapid emergence of variants (PubMed:11685239).
Generates mismatched G-G pairs, in that case, the polymerase first binds the deoxynucleotide followed by mismatch formation (PubMed:24617852).
Together with the viral DNA ligase, fills the single nucleotide gaps generated by the AP endonuclease (Probable). Binds DNA with high affinity via the helix alphaE (PubMed:24617852).
Curated4 PublicationsMiscellaneous
Catalytic activityi
- EC:2.7.7.71 Publication
Cofactori
Kineticsi
- KM=0.26 µM for dGTP1 Publication
- KM=0.46 µM for dATP1 Publication
- KM=9.4 µM for dCTP1 Publication
- KM=8.6 µM for dTTP1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 49 | Magnesium1 Publication1 Publication | 1 | |
Metal bindingi | 51 | Magnesium1 Publication1 Publication | 1 | |
Metal bindingi | 100 | Magnesium1 Publication1 Publication | 1 | |
Sitei | 115 | Stabilizes dGTP in a syn confromation to overcome the Watson-Crick base pairing constraint1 Publication | 1 |
GO - Molecular functioni
- DNA binding Source: UniProtKB-KW
- DNA-directed DNA polymerase activity Source: CACAO
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- DNA repair Source: UniProtKB-KW
Keywordsi
Molecular function | DNA-binding, DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase |
Biological process | DNA damage, DNA repair |
Ligand | Magnesium, Metal-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Repair DNA polymerase X (EC:2.7.7.72 Publications)Short name: Pol X Alternative name(s): AsfvPolX |
Gene namesi | Ordered Locus Names:Ba71V-97 ORF Names:O174L |
Organismi | African swine fever virus (strain Badajoz 1971 Vero-adapted) (Ba71V) (ASFV) |
Taxonomic identifieri | 10498 [NCBI] |
Taxonomic lineagei | Viruses › Varidnaviria › Bamfordvirae › Nucleocytoviricota › Pokkesviricetes › Asfuvirales › Asfarviridae › Asfivirus › |
Virus hosti | Ornithodoros (relapsing fever ticks) [TaxID: 6937] Sus scrofa (Pig) [TaxID: 9823] |
Proteomesi |
|
Subcellular locationi
- Virion 1 Publication Note: Found in association with viral nucleoid.1 Publication
Keywords - Cellular componenti
VirionPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 115 | H → A: Complete loss of MgdGTP binding and dG:dGTP ternary complex formation but not dG:dCTP ternary complex formation. 1 Publication | 1 | |
Mutagenesisi | 115 | H → D: 18x decreased dG:dGTP misincorporation. 1 Publication | 1 | |
Mutagenesisi | 115 | H → E: 36x decreased dG:dGTP misincorporation. 1 Publication | 1 | |
Mutagenesisi | 115 | H → F: Slower dG:dGTP misincorporation. 1 Publication | 1 | |
Mutagenesisi | 125 | R → A: Loss of DNA binding affinity. Decreased dG:dGTP misincorporation. 1 Publication | 1 | |
Mutagenesisi | 127 | R → A: Slower dG:dGTP misincorporation. 1 Publication | 1 | |
Mutagenesisi | 168 | R → A: Loss of DNA binding affinity. Decreased dGTP misincorporation. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000218798 | 1 – 174 | Repair DNA polymerase XAdd BLAST | 174 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 81 ↔ 86 | 1 Publication |
Keywords - PTMi
Disulfide bondStructurei
Secondary structure
3D structure databases
BMRBi | P42494 |
SMRi | P42494 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P42494 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 42 – 51 | Involved in ssDNA bindingBy similarity | 10 |
Domaini
Sequence similaritiesi
Family and domain databases
Gene3Di | 3.30.210.10, 1 hit 3.30.460.10, 1 hit |
InterProi | View protein in InterPro IPR019843, DNA_pol-X_BS IPR037160, DNA_Pol_thumb_sf IPR022312, DNA_pol_X IPR043519, NT_sf IPR029398, PolB_thumb |
PANTHERi | PTHR11276, PTHR11276, 1 hit |
Pfami | View protein in Pfam PF14791, DNA_pol_B_thumb, 1 hit |
SUPFAMi | SSF81301, SSF81301, 1 hit |
PROSITEi | View protein in PROSITE PS00522, DNA_POLYMERASE_X, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MLTLIQGKKI VNHLRSRLAF EYNGQLIKIL SKNIVAVGSL RREEKMLNDV
60 70 80 90 100
DLLIIVPEKK LLKHVLPNIR IKGLSFSVKV CGERKCVLFI EWEKKTYQLD
110 120 130 140 150
LFTALAEEKP YAIFHFTGPV SYLIRIRAAL KKKNYKLNQY GLFKNQTLVP
160 170
LKITTEKELI KELGFTYRIP KKRL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U18466 Genomic DNA Translation: AAA65326.1 |
RefSeqi | NP_042790.1, NC_001659.2 |
Genome annotation databases
GeneIDi | 22220326 |
KEGGi | vg:22220326 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U18466 Genomic DNA Translation: AAA65326.1 |
RefSeqi | NP_042790.1, NC_001659.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1JAJ | NMR | - | A | 1-174 | [»] | |
1JQR | NMR | - | A | 1-174 | [»] | |
2M2T | NMR | - | A | 1-174 | [»] | |
2M2U | NMR | - | A | 1-174 | [»] | |
2M2V | NMR | - | A | 1-174 | [»] | |
2M2W | NMR | - | A | 1-174 | [»] | |
5HR9 | X-ray | 2.20 | A/B | 1-174 | [»] | |
5HRB | X-ray | 1.70 | A | 1-174 | [»] | |
5HRD | X-ray | 1.80 | A/B/C/D | 1-174 | [»] | |
5HRE | X-ray | 1.75 | A | 1-174 | [»] | |
5HRF | X-ray | 2.25 | A/B | 1-174 | [»] | |
5HRG | X-ray | 2.00 | A/B | 1-174 | [»] | |
5HRH | X-ray | 3.00 | A/B | 1-174 | [»] | |
5HRI | X-ray | 2.20 | A/B | 1-174 | [»] | |
5HRK | X-ray | 2.90 | A/B | 1-174 | [»] | |
5HRL | X-ray | 2.40 | A/B | 1-174 | [»] | |
5XM8 | X-ray | 2.55 | A/B/C/D | 1-174 | [»] | |
5XM9 | X-ray | 3.05 | A/B/C/D | 1-174 | [»] | |
5XMA | X-ray | 3.80 | A/B | 1-174 | [»] | |
BMRBi | P42494 | |||||
SMRi | P42494 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Genome annotation databases
GeneIDi | 22220326 |
KEGGi | vg:22220326 |
Miscellaneous databases
EvolutionaryTracei | P42494 |
Family and domain databases
Gene3Di | 3.30.210.10, 1 hit 3.30.460.10, 1 hit |
InterProi | View protein in InterPro IPR019843, DNA_pol-X_BS IPR037160, DNA_Pol_thumb_sf IPR022312, DNA_pol_X IPR043519, NT_sf IPR029398, PolB_thumb |
PANTHERi | PTHR11276, PTHR11276, 1 hit |
Pfami | View protein in Pfam PF14791, DNA_pol_B_thumb, 1 hit |
SUPFAMi | SSF81301, SSF81301, 1 hit |
PROSITEi | View protein in PROSITE PS00522, DNA_POLYMERASE_X, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DPOLX_ASFB7 | |
Accessioni | P42494Primary (citable) accession number: P42494 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | November 1, 1995 | |
Last modified: | February 23, 2022 | |
This is version 105 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families