UniProtKB - P42435 (NASD_BACSU)
Protein
Nitrite reductase [NAD(P)H]
Gene
nasD
Organism
Bacillus subtilis (strain 168)
Status
Functioni
Required for nitrite assimilation.1 Publication
Catalytic activityi
Cofactori
Protein has several cofactor binding sites:- sirohemeNote: Binds 1 siroheme per subunit.
- [4Fe-4S] clusterNote: Binds 1 [4Fe-4S] cluster per subunit.
- FAD
: nitrate reduction (assimilation) Pathwayi
This protein is involved in the pathway nitrate reduction (assimilation), which is part of Nitrogen metabolism.View all proteins of this organism that are known to be involved in the pathway nitrate reduction (assimilation) and in Nitrogen metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 635 | Iron-sulfur (4Fe-4S)By similarity | 1 | |
Metal bindingi | 641 | Iron-sulfur (4Fe-4S)By similarity | 1 | |
Metal bindingi | 675 | Iron-sulfur (4Fe-4S)By similarity | 1 | |
Metal bindingi | 679 | Iron (siroheme axial ligand)By similarity | 1 | |
Metal bindingi | 679 | Iron-sulfur (4Fe-4S)By similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 43 – 79 | FADSequence analysisAdd BLAST | 37 | |
Nucleotide bindingi | 193 – 223 | NAD or NADPSequence analysisAdd BLAST | 31 |
GO - Molecular functioni
- 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
- flavin adenine dinucleotide binding Source: InterPro
- heme binding Source: InterPro
- metal ion binding Source: UniProtKB-KW
- NADP binding Source: InterPro
- nitrite reductase [NAD(P)H] activity Source: UniProtKB-EC
GO - Biological processi
- nitrate assimilation Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Nitrate assimilation |
Ligand | 4Fe-4S, FAD, Flavoprotein, Heme, Iron, Iron-sulfur, Metal-binding, NADP |
Enzyme and pathway databases
BioCyci | BSUB:BSU03300-MONOMER |
UniPathwayi | UPA00653 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:nasD Synonyms:nasBC, nirB Ordered Locus Names:BSU03300 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000199961 | 1 – 805 | Nitrite reductase [NAD(P)H]Add BLAST | 805 |
Proteomic databases
jPOSTi | P42435 |
PaxDbi | P42435 |
PRIDEi | P42435 |
Expressioni
Inductioni
Positively regulated by TnrA under nitrogen-limited conditions. Induced by ArfM.3 Publications
Interactioni
Subunit structurei
Homodimer.
By similarityProtein-protein interaction databases
STRINGi | 224308.BSU03300 |
Family & Domainsi
Sequence similaritiesi
Belongs to the nitrite and sulfite reductase 4Fe-4S domain family.Curated
Phylogenomic databases
eggNOGi | COG1251, Bacteria |
InParanoidi | P42435 |
OMAi | FAQVDPW |
PhylomeDBi | P42435 |
Family and domain databases
Gene3Di | 1.10.10.1100, 1 hit 3.30.390.30, 1 hit 3.50.50.60, 2 hits |
InterProi | View protein in InterPro IPR007419, BFD-like_2Fe2S-bd_dom IPR041854, BFD-like_2Fe2S-bd_dom_sf IPR036188, FAD/NAD-bd_sf IPR023753, FAD/NAD-binding_dom IPR016156, FAD/NAD-linked_Rdtase_dimer_sf IPR005117, NiRdtase/SiRdtase_haem-b_fer IPR036136, Nit/Sulf_reduc_fer-like_dom_sf IPR012744, Nitri_red_NirB IPR017121, Nitrite_Rdtase_lsu IPR006067, NO2/SO3_Rdtase_4Fe4S_dom IPR006066, NO2/SO3_Rdtase_FeS/sirohaem_BS IPR041575, Rubredoxin_C |
Pfami | View protein in Pfam PF04324, Fer2_BFD, 2 hits PF01077, NIR_SIR, 1 hit PF03460, NIR_SIR_ferr, 1 hit PF07992, Pyr_redox_2, 1 hit PF18267, Rubredoxin_C, 1 hit |
PIRSFi | PIRSF037149, NirB, 1 hit |
PRINTSi | PR00397, SIROHAEM |
SUPFAMi | SSF51905, SSF51905, 2 hits SSF55124, SSF55124, 1 hit |
TIGRFAMsi | TIGR02374, nitri_red_nirB, 1 hit |
i Sequence
Sequence statusi: Complete.
P42435-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGKKQLVLVG NGMAGVRAIE EILSVAKDEF QITIFGAEPH PNYNRILLSK
60 70 80 90 100
VLQGDTDIKD ITLNDWDWYE ENNIQLYTNE TVIKVDTENK TVITDADRIQ
110 120 130 140 150
PYDELILATG SVPFILPIPG ADKKGVTAFR DIKDTDTMLA ASKQYKKAAV
160 170 180 190 200
IGGGLLGLEA ARGLLNLGMD VSVIHLAPFL MERQLDATAG RLLQNELEKQ
210 220 230 240 250
GMTFLLEKQT EEIVGDDRVE GLRFKDGTSI EADLVVMAVG IRPNTTLGAE
260 270 280 290 300
SGIPVNRGII VNDYMQTEIP HIYAVGECAE HRGIAYGLVA PLYEQAKVLA
310 320 330 340 350
KHMCGIETKP YEGSVLSTQL KVSGVEVFSA GDFNESEEKK AIKVFDEQDG
360 370 380 390 400
IYKKIVLRGN QIVGAVLFGD SSEGNRLFSM IQKEADISET SKISILQPLS
410 420 430 440 450
QEAGTSITAA MSDDEIICGC NGVSKGAIIQ AIQEKGCSST DEIKACTGAS
460 470 480 490 500
RSCGGCKPLV EEILQHTLGS DFDASAQKEA ICGCTTLSRD EVVEEIKAKG
510 520 530 540 550
LSHTREVMNV LGWKTPEGCS KCRPALNYYL GMINPTKYED DRTSRFVNER
560 570 580 590 600
MHANIQKDGT YSVVPRMYGG VTNSTDLRKI ADVVDKYEIP LVKMTGGQRI
610 620 630 640 650
DLIGVKKEDL PKVWEDLDMP SGYAYGKTLR TVKTCVGEQF CRFGTQDSMA
660 670 680 690 700
LGIALEKKFE GLNTPHKVKM AVSACPRNCA ESGIKDLGVV GIDGGWELYV
710 720 730 740 750
GGNGGTHLRA GDLLMKVKTN EEVLEYAGAY LQYYRETANY LERTSAWLER
760 770 780 790 800
VGLSHVQSVL NDPEKRQELN GRMNETLSVH KDPWKDFLED KQTSKELFEN
VVTTS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D30689 Genomic DNA Translation: BAA06354.1 D50453 Genomic DNA Translation: BAA08964.1 AL009126 Genomic DNA Translation: CAB12124.1 |
PIRi | I40029 |
RefSeqi | NP_388212.1, NC_000964.3 WP_003234637.1, NZ_JNCM01000030.1 |
Genome annotation databases
EnsemblBacteriai | CAB12124; CAB12124; BSU03300 |
GeneIDi | 938329 |
KEGGi | bsu:BSU03300 |
PATRICi | fig|224308.179.peg.344 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D30689 Genomic DNA Translation: BAA06354.1 D50453 Genomic DNA Translation: BAA08964.1 AL009126 Genomic DNA Translation: CAB12124.1 |
PIRi | I40029 |
RefSeqi | NP_388212.1, NC_000964.3 WP_003234637.1, NZ_JNCM01000030.1 |
3D structure databases
SMRi | P42435 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 224308.BSU03300 |
Proteomic databases
jPOSTi | P42435 |
PaxDbi | P42435 |
PRIDEi | P42435 |
Genome annotation databases
EnsemblBacteriai | CAB12124; CAB12124; BSU03300 |
GeneIDi | 938329 |
KEGGi | bsu:BSU03300 |
PATRICi | fig|224308.179.peg.344 |
Phylogenomic databases
eggNOGi | COG1251, Bacteria |
InParanoidi | P42435 |
OMAi | FAQVDPW |
PhylomeDBi | P42435 |
Enzyme and pathway databases
UniPathwayi | UPA00653 |
BioCyci | BSUB:BSU03300-MONOMER |
Family and domain databases
Gene3Di | 1.10.10.1100, 1 hit 3.30.390.30, 1 hit 3.50.50.60, 2 hits |
InterProi | View protein in InterPro IPR007419, BFD-like_2Fe2S-bd_dom IPR041854, BFD-like_2Fe2S-bd_dom_sf IPR036188, FAD/NAD-bd_sf IPR023753, FAD/NAD-binding_dom IPR016156, FAD/NAD-linked_Rdtase_dimer_sf IPR005117, NiRdtase/SiRdtase_haem-b_fer IPR036136, Nit/Sulf_reduc_fer-like_dom_sf IPR012744, Nitri_red_NirB IPR017121, Nitrite_Rdtase_lsu IPR006067, NO2/SO3_Rdtase_4Fe4S_dom IPR006066, NO2/SO3_Rdtase_FeS/sirohaem_BS IPR041575, Rubredoxin_C |
Pfami | View protein in Pfam PF04324, Fer2_BFD, 2 hits PF01077, NIR_SIR, 1 hit PF03460, NIR_SIR_ferr, 1 hit PF07992, Pyr_redox_2, 1 hit PF18267, Rubredoxin_C, 1 hit |
PIRSFi | PIRSF037149, NirB, 1 hit |
PRINTSi | PR00397, SIROHAEM |
SUPFAMi | SSF51905, SSF51905, 2 hits SSF55124, SSF55124, 1 hit |
TIGRFAMsi | TIGR02374, nitri_red_nirB, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NASD_BACSU | |
Accessioni | P42435Primary (citable) accession number: P42435 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | November 1, 1995 | |
Last modified: | December 2, 2020 | |
This is version 162 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families