UniProtKB - P42434 (NASC_BACSU)
Protein
Assimilatory nitrate reductase catalytic subunit
Gene
nasC
Organism
Bacillus subtilis (strain 168)
Status
Functioni
Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria.
Cofactori
Protein has several cofactor binding sites:- [4Fe-4S] clusterCuratedNote: Binds 1 [4Fe-4S] cluster.Curated
- Mo-bis(molybdopterin guanine dinucleotide)By similarityNote: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.By similarity
: nitrate reduction (denitrification) Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes dinitrogen from nitrate.Proteins known to be involved in the 4 steps of the subpathway in this organism are:
- Assimilatory nitrate reductase catalytic subunit (nasC)
- no protein annotated in this organism
- no protein annotated in this organism
- no protein annotated in this organism
View all proteins of this organism that are known to be involved in the subpathway that synthesizes dinitrogen from nitrate, the pathway nitrate reduction (denitrification) and in Nitrogen metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 26 | Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation | 1 | |
Metal bindingi | 29 | Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation | 1 | |
Metal bindingi | 33 | Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation | 1 | |
Metal bindingi | 63 | Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
- molybdopterin cofactor binding Source: InterPro
- oxidoreductase activity Source: UniProtKB-KW
GO - Biological processi
- denitrification pathway Source: UniProtKB-UniPathway
- nitrate assimilation Source: UniProtKB-KW
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Nitrate assimilation |
Ligand | 4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum |
Enzyme and pathway databases
BioCyci | BSUB:BSU03310-MONOMER |
UniPathwayi | UPA00652;UER00706 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:nasC Synonyms:narB, nasBB Ordered Locus Names:BSU03310 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000063239 | 1 – 710 | Assimilatory nitrate reductase catalytic subunitAdd BLAST | 710 |
Proteomic databases
PaxDbi | P42434 |
PRIDEi | P42434 |
Expressioni
Inductioni
Positively regulated by TnrA under nitrogen-limited conditions.2 Publications
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 19 – 77 | 4Fe-4S Mo/W bis-MGD-typePROSITE-ProRule annotationAdd BLAST | 59 |
Sequence similaritiesi
Belongs to the prokaryotic molybdopterin-containing oxidoreductase family.Curated
Phylogenomic databases
eggNOGi | COG0243, Bacteria |
InParanoidi | P42434 |
OMAi | AANIDTN |
PhylomeDBi | P42434 |
Family and domain databases
InterProi | View protein in InterPro IPR009010, Asp_de-COase-like_dom_sf IPR006657, MoPterin_dinucl-bd_dom IPR006656, Mopterin_OxRdtase IPR006963, Mopterin_OxRdtase_4Fe-4S_dom IPR006655, Mopterin_OxRdtase_prok_CS IPR027467, MopterinOxRdtase_cofactor_BS |
Pfami | View protein in Pfam PF04879, Molybdop_Fe4S4, 1 hit PF00384, Molybdopterin, 1 hit PF01568, Molydop_binding, 1 hit |
SMARTi | View protein in SMART SM00926, Molybdop_Fe4S4, 1 hit |
SUPFAMi | SSF50692, SSF50692, 1 hit |
PROSITEi | View protein in PROSITE PS51669, 4FE4S_MOW_BIS_MGD, 1 hit PS00551, MOLYBDOPTERIN_PROK_1, 1 hit PS00490, MOLYBDOPTERIN_PROK_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P42434-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTERLLRYFR DKQQDVQSEK TYDTQCPFCS MQCKMQLVEQ TIVTRKKYTA
60 70 80 90 100
IGIDNPTTQG RLCIKGMNAH QHALNSSRIT RPLLKKNGEF MPVSWEEALN
110 120 130 140 150
HIKDQVTMIQ TEHGHDAMAV YGSASITNEE AYLLGKFARV GLQTKYIDYN
160 170 180 190 200
GRLCMSAAAT AANQTFGADR GLTNPLSDIP HTRVIILAGT NIAECQPTIM
210 220 230 240 250
PYFEKAKENG AYFIAIDPRE TATTKIADLH LKIKPGTDAA LANGLVKIII
260 270 280 290 300
DEQLINEDFI QSRTNGFEEL KQHTDSLDLN DIAEQTSVSL VDIRKAAVKF
310 320 330 340 350
AKETSGMLFT ARGIEQQTDG TAAVKGFLNM VLITGKIGKP YSGYGAITGQ
360 370 380 390 400
GNGQGAREHG QKADQLPGYR SIENEEHRAH IAKVWGIHQD ELPRKGVSAY
410 420 430 440 450
EMMEKINDGD IKGLFLMCSN PAVSSPNANL VKKALRRLTF FVAIDLFISE
460 470 480 490 500
TAKYADVILP ASSYLEDEGT MTNVEGRVTL REASRPCPGE AKHDWQIICD
510 520 530 540 550
LASALGKGRY FSYTSAEDIF NELREASRGG IADYSGISYG RLRREGGIHW
560 570 580 590 600
PCPESDHPGT GRLFTESFAH PDQKAALSVI PNEPPVPKEK PTADYPLYLT
610 620 630 640 650
TGRVMSHYLT GVQTRKSAAL AARHFESFME IHPQTAATYN IEDRVLVKIE
660 670 680 690 700
SPRGSITVRS KLSEQIRKDT VFVPIHWADA QNVNDLIGEA LDPACKMPGF
710
KVCAVRIIPI
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 391 | E → D in BAA08965 (PubMed:8969502).Curated | 1 | |
Sequence conflicti | 391 | E → D in BAA06353 (PubMed:7868621).Curated | 1 | |
Sequence conflicti | 402 | M → V in BAA08965 (PubMed:8969502).Curated | 1 | |
Sequence conflicti | 402 | M → V in BAA06353 (PubMed:7868621).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D50453 Genomic DNA Translation: BAA08965.1 AL009126 Genomic DNA Translation: CAB12125.2 D30689 Genomic DNA Translation: BAA06353.1 |
PIRi | E69665 |
RefSeqi | NP_388213.2, NC_000964.3 WP_003246484.1, NZ_JNCM01000030.1 |
Genome annotation databases
EnsemblBacteriai | CAB12125; CAB12125; BSU_03310 |
GeneIDi | 938321 |
KEGGi | bsu:BSU03310 |
PATRICi | fig|224308.179.peg.345 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D50453 Genomic DNA Translation: BAA08965.1 AL009126 Genomic DNA Translation: CAB12125.2 D30689 Genomic DNA Translation: BAA06353.1 |
PIRi | E69665 |
RefSeqi | NP_388213.2, NC_000964.3 WP_003246484.1, NZ_JNCM01000030.1 |
3D structure databases
SMRi | P42434 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 224308.BSU03310 |
Proteomic databases
PaxDbi | P42434 |
PRIDEi | P42434 |
Genome annotation databases
EnsemblBacteriai | CAB12125; CAB12125; BSU_03310 |
GeneIDi | 938321 |
KEGGi | bsu:BSU03310 |
PATRICi | fig|224308.179.peg.345 |
Phylogenomic databases
eggNOGi | COG0243, Bacteria |
InParanoidi | P42434 |
OMAi | AANIDTN |
PhylomeDBi | P42434 |
Enzyme and pathway databases
UniPathwayi | UPA00652;UER00706 |
BioCyci | BSUB:BSU03310-MONOMER |
Family and domain databases
InterProi | View protein in InterPro IPR009010, Asp_de-COase-like_dom_sf IPR006657, MoPterin_dinucl-bd_dom IPR006656, Mopterin_OxRdtase IPR006963, Mopterin_OxRdtase_4Fe-4S_dom IPR006655, Mopterin_OxRdtase_prok_CS IPR027467, MopterinOxRdtase_cofactor_BS |
Pfami | View protein in Pfam PF04879, Molybdop_Fe4S4, 1 hit PF00384, Molybdopterin, 1 hit PF01568, Molydop_binding, 1 hit |
SMARTi | View protein in SMART SM00926, Molybdop_Fe4S4, 1 hit |
SUPFAMi | SSF50692, SSF50692, 1 hit |
PROSITEi | View protein in PROSITE PS51669, 4FE4S_MOW_BIS_MGD, 1 hit PS00551, MOLYBDOPTERIN_PROK_1, 1 hit PS00490, MOLYBDOPTERIN_PROK_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NASC_BACSU | |
Accessioni | P42434Primary (citable) accession number: P42434 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | June 16, 2009 | |
Last modified: | April 7, 2021 | |
This is version 145 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families