UniProtKB - P42433 (NASB_BACSU)
Protein
Assimilatory nitrate reductase electron transfer subunit
Gene
nasB
Organism
Bacillus subtilis (strain 168)
Status
Functioni
Required for nitrate assimilation.1 Publication
Cofactori
FADCurated
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 43 – 79 | FADSequence analysisAdd BLAST | 37 | |
Nucleotide bindingi | 193 – ? | NAD or NADPSequence analysis |
GO - Molecular functioni
- flavin adenine dinucleotide binding Source: InterPro
- NADP binding Source: InterPro
- nitrite reductase [NAD(P)H] activity Source: InterPro
GO - Biological processi
- nitrate assimilation Source: UniProtKB-KW
Keywordsi
Biological process | Electron transport, Nitrate assimilation, Transport |
Ligand | FAD, Flavoprotein, NADP |
Enzyme and pathway databases
BioCyci | BSUB:BSU03320-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Assimilatory nitrate reductase electron transfer subunit |
Gene namesi | Name:nasB Synonyms:nasBA Ordered Locus Names:BSU03320 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000096736 | 1 – 771 | Assimilatory nitrate reductase electron transfer subunitAdd BLAST | 771 |
Proteomic databases
PaxDbi | P42433 |
PRIDEi | P42433 |
Expressioni
Inductioni
Positively regulated by TnrA under nitrogen-limited conditions.2 Publications
Family & Domainsi
Phylogenomic databases
eggNOGi | COG1251, Bacteria |
InParanoidi | P42433 |
OMAi | ETICQCN |
PhylomeDBi | P42433 |
Family and domain databases
Gene3Di | 1.10.10.1100, 1 hit 3.30.390.30, 1 hit 3.50.50.60, 2 hits |
InterProi | View protein in InterPro IPR007419, BFD-like_2Fe2S-bd_dom IPR041854, BFD-like_2Fe2S-bd_dom_sf IPR036188, FAD/NAD-bd_sf IPR023753, FAD/NAD-binding_dom IPR016156, FAD/NAD-linked_Rdtase_dimer_sf IPR005117, NiRdtase/SiRdtase_haem-b_fer IPR036136, Nit/Sulf_reduc_fer-like_dom_sf IPR012744, Nitri_red_NirB IPR017121, Nitrite_Rdtase_lsu IPR041575, Rubredoxin_C |
Pfami | View protein in Pfam PF04324, Fer2_BFD, 2 hits PF03460, NIR_SIR_ferr, 1 hit PF07992, Pyr_redox_2, 1 hit PF18267, Rubredoxin_C, 1 hit |
PIRSFi | PIRSF037149, NirB, 1 hit |
SUPFAMi | SSF51905, SSF51905, 2 hits SSF55124, SSF55124, 1 hit |
TIGRFAMsi | TIGR02374, nitri_red_nirB, 1 hit |
i Sequence
Sequence statusi: Complete.
P42433-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKKQRLVLAG NGMAGIRCIE EVLKLNRHMF EIVIFGSEPH PNYNRILLSS
60 70 80 90 100
VLQGEASLDD ITLNSKDWYD KHGITLYTGE TVIQIDTDQQ QVITDRKRTL
110 120 130 140 150
SYDKLIVATG SSPHILPIPG ADKKGVYGFR TIEDCQALMN MAQHFQKAAV
160 170 180 190 200
IGAGLLGLEA AVGLQHLGMD VSVIHHSAGI MQKQLDQTAA RLLQTELEQK
210 220 230 240 250
GLTFLLEKDT VSISGATKAD RIHFKDGSSL KADLIVMAAG VKPNIELAVS
260 270 280 290 300
AGIKVNRGII VNDFMQTSEP NIYAVGECAE HNGTVYGLVA PLYEQGKALA
310 320 330 340 350
SHICGVPCEE YQGSAPSAAL KIAGIDVWSA GKIQEDERTT SIKIYDEQAG
360 370 380 390 400
VYKKALFVDD KLAGVILFGD TRDKQRLLDS LLKQRDISIA KKQIIEPETS
410 420 430 440 450
GPLFESMPSS ETICQCNTVT KGAIEDAVHT NSLTTVEEVK HCTKATGSCG
460 470 480 490 500
GCKPLVEDLL RYMTNSEYTK PASTPSFCSC TDFTEDDIIA ELQRRPFTNP
510 520 530 540 550
AEVMNQLDWK TKNGCSTCVP AIQYYLEMLY PGFVQPEPAT EETCILIPQM
560 570 580 590 600
YGGRTNAEQL RTIANIIEAY SIPDVSITHG QRLKLSGIKP ADLPNMKKDL
610 620 630 640 650
KMPVYTNEHR HALQSIKACT CGQNRSIQQL AAQIERQLEM LPLPAPISIS
660 670 680 690 700
LSCETDCTEA ALQDVGAIRT QAGWDIHIGG VRGTHARSGA LFCVTENEDS
710 720 730 740 750
TAGMIKGLIQ YYRETAHYLE GVHQWIDRLG IVHIREVLFE EDLRAQLLES
760 770
LQTDLSLIQN PTVETGAYKK G
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 363 – 364 | AG → R in BAA06352 (PubMed:7868621).Curated | 2 | |
Sequence conflicti | 363 – 364 | AG → R in BAA08966 (PubMed:8969502).Curated | 2 | |
Sequence conflicti | 716 | A → D in BAA06352 (PubMed:7868621).Curated | 1 | |
Sequence conflicti | 716 | A → D in BAA08966 (PubMed:8969502).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D30689 Genomic DNA Translation: BAA06352.1 D50453 Genomic DNA Translation: BAA08966.1 AL009126 Genomic DNA Translation: CAB12126.2 |
PIRi | I40027 |
RefSeqi | NP_388214.2, NC_000964.3 WP_003246389.1, NZ_JNCM01000030.1 |
Genome annotation databases
EnsemblBacteriai | CAB12126; CAB12126; BSU03320 |
GeneIDi | 938328 |
KEGGi | bsu:BSU03320 |
PATRICi | fig|224308.179.peg.346 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D30689 Genomic DNA Translation: BAA06352.1 D50453 Genomic DNA Translation: BAA08966.1 AL009126 Genomic DNA Translation: CAB12126.2 |
PIRi | I40027 |
RefSeqi | NP_388214.2, NC_000964.3 WP_003246389.1, NZ_JNCM01000030.1 |
3D structure databases
SMRi | P42433 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 224308.BSU03320 |
Proteomic databases
PaxDbi | P42433 |
PRIDEi | P42433 |
Genome annotation databases
EnsemblBacteriai | CAB12126; CAB12126; BSU03320 |
GeneIDi | 938328 |
KEGGi | bsu:BSU03320 |
PATRICi | fig|224308.179.peg.346 |
Phylogenomic databases
eggNOGi | COG1251, Bacteria |
InParanoidi | P42433 |
OMAi | ETICQCN |
PhylomeDBi | P42433 |
Enzyme and pathway databases
BioCyci | BSUB:BSU03320-MONOMER |
Family and domain databases
Gene3Di | 1.10.10.1100, 1 hit 3.30.390.30, 1 hit 3.50.50.60, 2 hits |
InterProi | View protein in InterPro IPR007419, BFD-like_2Fe2S-bd_dom IPR041854, BFD-like_2Fe2S-bd_dom_sf IPR036188, FAD/NAD-bd_sf IPR023753, FAD/NAD-binding_dom IPR016156, FAD/NAD-linked_Rdtase_dimer_sf IPR005117, NiRdtase/SiRdtase_haem-b_fer IPR036136, Nit/Sulf_reduc_fer-like_dom_sf IPR012744, Nitri_red_NirB IPR017121, Nitrite_Rdtase_lsu IPR041575, Rubredoxin_C |
Pfami | View protein in Pfam PF04324, Fer2_BFD, 2 hits PF03460, NIR_SIR_ferr, 1 hit PF07992, Pyr_redox_2, 1 hit PF18267, Rubredoxin_C, 1 hit |
PIRSFi | PIRSF037149, NirB, 1 hit |
SUPFAMi | SSF51905, SSF51905, 2 hits SSF55124, SSF55124, 1 hit |
TIGRFAMsi | TIGR02374, nitri_red_nirB, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NASB_BACSU | |
Accessioni | P42433Primary (citable) accession number: P42433 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | June 16, 2009 | |
Last modified: | December 2, 2020 | |
This is version 145 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |