UniProtKB - P42331 (RHG25_HUMAN)
Protein
Rho GTPase-activating protein 25
Gene
ARHGAP25
Organism
Homo sapiens (Human)
Status
Functioni
GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state.By similarity
GO - Molecular functioni
- GTPase activator activity Source: GO_Central
GO - Biological processi
- actin filament organization Source: UniProtKB
- activation of GTPase activity Source: GO_Central
- negative regulation of small GTPase mediated signal transduction Source: UniProtKB
- phagocytosis, engulfment Source: UniProtKB
- regulation of small GTPase mediated signal transduction Source: Reactome
- signal transduction Source: InterPro
Keywordsi
Molecular function | GTPase activation |
Enzyme and pathway databases
PathwayCommonsi | P42331 |
Reactomei | R-HSA-194840, Rho GTPase cycle |
Names & Taxonomyi
Protein namesi | Recommended name: Rho GTPase-activating protein 25Alternative name(s): Rho-type GTPase-activating protein 25 |
Gene namesi | Name:ARHGAP25 Synonyms:KIAA0053 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:28951, ARHGAP25 |
MIMi | 610587, gene |
neXtProti | NX_P42331 |
VEuPathDBi | HostDB:ENSG00000163219.11 |
Subcellular locationi
Cytosol
- cytosol Source: Reactome
Plasma Membrane
- phagocytic cup Source: UniProtKB
Pathology & Biotechi
Organism-specific databases
DisGeNETi | 9938 |
OpenTargetsi | ENSG00000163219 |
PharmGKBi | PA134941737 |
Miscellaneous databases
Pharosi | P42331, Tbio |
Genetic variation databases
BioMutai | ARHGAP25 |
DMDMi | 238054314 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000056716 | 1 – 645 | Rho GTPase-activating protein 25Add BLAST | 645 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 362 | PhosphoserineCombined sources | 1 | |
Modified residuei | 395 | PhosphoserineCombined sources | 1 | |
Modified residuei | 406 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 536 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | P42331 |
MassIVEi | P42331 |
MaxQBi | P42331 |
PaxDbi | P42331 |
PeptideAtlasi | P42331 |
PRIDEi | P42331 |
ProteomicsDBi | 20155 33928 55505 [P42331-1] 55506 [P42331-2] 55507 [P42331-3] 55508 [P42331-4] |
PTM databases
CarbonylDBi | P42331 |
iPTMneti | P42331 |
PhosphoSitePlusi | P42331 |
Expressioni
Gene expression databases
Bgeei | ENSG00000163219, Expressed in blood and 195 other tissues |
ExpressionAtlasi | P42331, baseline and differential |
Genevisiblei | P42331, HS |
Organism-specific databases
HPAi | ENSG00000163219, Group enriched (blood, lymphoid tissue) |
Interactioni
Binary interactionsi
Hide detailsIsoform 2 [P42331-2]
With | #Exp. | IntAct |
---|---|---|
CCT6A [P40227] | 3 | EBI-21499901,EBI-356687 |
CDHR3 [Q6ZTQ4] | 3 | EBI-21499901,EBI-12143631 |
MAGEB6 [Q8N7X4] | 3 | EBI-21499901,EBI-6447163 |
VDAC2 [P45880] | 3 | EBI-21499901,EBI-354022 |
Protein-protein interaction databases
BioGRIDi | 115264, 30 interactors |
IntActi | P42331, 25 interactors |
MINTi | P42331 |
STRINGi | 9606.ENSP00000386911 |
Miscellaneous databases
RNActi | P42331, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P42331 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P42331 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 46 – 151 | PHPROSITE-ProRule annotationAdd BLAST | 106 | |
Domaini | 159 – 353 | Rho-GAPPROSITE-ProRule annotationAdd BLAST | 195 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 541 – 644 | Sequence analysisAdd BLAST | 104 |
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG4270, Eukaryota |
GeneTreei | ENSGT00950000183015 |
HOGENOMi | CLU_020795_1_0_1 |
InParanoidi | P42331 |
OMAi | DVKPQGC |
OrthoDBi | 917149at2759 |
PhylomeDBi | P42331 |
TreeFami | TF323577 |
Family and domain databases
Gene3Di | 1.10.555.10, 1 hit 2.30.29.30, 1 hit |
InterProi | View protein in InterPro IPR011993, PH-like_dom_sf IPR001849, PH_domain IPR008936, Rho_GTPase_activation_prot IPR000198, RhoGAP_dom |
Pfami | View protein in Pfam PF00169, PH, 1 hit PF00620, RhoGAP, 1 hit |
SMARTi | View protein in SMART SM00233, PH, 1 hit SM00324, RhoGAP, 1 hit |
SUPFAMi | SSF48350, SSF48350, 1 hit |
PROSITEi | View protein in PROSITE PS50003, PH_DOMAIN, 1 hit PS50238, RHOGAP, 1 hit |
s (6+)i Sequence
Sequence statusi: Complete.
This entry describes 6 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 6 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: P42331-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSLKLPRNWD FNLKVEAAKI ARSRSVMTGE QMAAFHPSST PNPLERPIKM
60 70 80 90 100
GWLKKQRSIV KNWQQRYFVL RAQQLYYYKD EEDTKPQGCM YLPGCTIKEI
110 120 130 140 150
ATNPEEAGKF VFEIIPASWD QNRMGQDSYV LMASSQAEME EWVKFLRRVA
160 170 180 190 200
GTPCGVFGQR LDETVAYEQK FGPHLVPILV EKCAEFILEH GRNEEGIFRL
210 220 230 240 250
PGQDNLVKQL RDAFDAGERP SFDRDTDVHT VASLLKLYLR DLPEPVVPWS
260 270 280 290 300
QYEGFLLCGQ LTNADEAKAQ QELMKQLSIL PRDNYSLLSY ICRFLHEIQL
310 320 330 340 350
NCAVNKMSVD NLATVIGVNL IRSKVEDPAV IMRGTPQIQR VMTMMIRDHE
360 370 380 390 400
VLFPKSKDIP LSPPAQKNDP KKAPVARSSV GWDATEDLRI SRTDSFSSMT
410 420 430 440 450
SDSDTTSPTG QQPSDAFPED SSKVPREKPG DWKMQSRKRT QTLPNRKCFL
460 470 480 490 500
TSAFQGANSS KMEIFKNEFW SPSSEAKAGE GHRRTMSQDL RQLSDSQRTS
510 520 530 540 550
TYDNVPSLPG SPGEEASALS SQACDSKGDT LASPNSETGP GKKNSGEEEI
560 570 580 590 600
DSLQRMVQEL RKEIETQKQM YEEQIKNLEK ENYDVWAKVV RLNEELEKEK
610 620 630 640
KKSAALEISL RNMERSREDV EKRNKALEEE VKEFVKSMKE PKTEA
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketH7C4P1 | H7C4P1_HUMAN | Rho GTPase-activating protein 25 | ARHGAP25 | 505 | Annotation score: | ||
C9JB56 | C9JB56_HUMAN | Rho GTPase-activating protein 25 | ARHGAP25 | 339 | Annotation score: | ||
B7Z8K7 | B7Z8K7_HUMAN | Rho GTPase-activating protein 25 | ARHGAP25 | 283 | Annotation score: | ||
F8WAV9 | F8WAV9_HUMAN | Rho GTPase-activating protein 25 | ARHGAP25 | 85 | Annotation score: | ||
F8WDF6 | F8WDF6_HUMAN | Rho GTPase-activating protein 25 | ARHGAP25 | 139 | Annotation score: |
Sequence cautioni
The sequence BAA06125 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_049142 | 192 | R → W. Corresponds to variant dbSNP:rs3749130Ensembl. | 1 | |
Natural variantiVAR_049143 | 555 | R → S1 PublicationCorresponds to variant dbSNP:rs4241344Ensembl. | 1 | |
Natural variantiVAR_049144 | 556 | M → T1 PublicationCorresponds to variant dbSNP:rs10177248Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_037345 | 1 – 19 | MSLKL…VEAAK → MSLGQSACLFLS in isoform 2, isoform 3 and isoform 6. 2 PublicationsAdd BLAST | 19 | |
Alternative sequenceiVSP_045391 | 117 – 155 | Missing in isoform 5. 1 PublicationAdd BLAST | 39 | |
Alternative sequenceiVSP_010275 | 155 | G → GA in isoform 2, isoform 4 and isoform 6. 2 Publications | 1 | |
Alternative sequenceiVSP_010276 | 400 – 645 | TSDSD…PKTEA → VRCREPSCFHWVLPLVQAIP CKACSRVAIWGVLGDAVAVG AAATDSSEHTLKAWPLSKSS FYWHL in isoform 2. 1 PublicationAdd BLAST | 246 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D29642 mRNA Translation: BAA06125.2 Different initiation. AK290396 mRNA Translation: BAF83085.1 AK297056 mRNA Translation: BAH12484.1 AC097495 Genomic DNA No translation available. AC105054 Genomic DNA No translation available. AC112700 Genomic DNA No translation available. CH471053 Genomic DNA Translation: EAW99866.1 BC039591 mRNA Translation: AAH39591.1 |
CCDSi | CCDS33214.2 [P42331-4] CCDS46312.1 [P42331-3] CCDS54363.1 [P42331-5] CCDS54364.1 [P42331-6] |
PIRi | C59430 |
RefSeqi | NP_001007232.2, NM_001007231.2 [P42331-4] NP_001159748.1, NM_001166276.1 [P42331-6] NP_001159749.1, NM_001166277.1 [P42331-5] NP_055697.1, NM_014882.2 [P42331-3] XP_005264732.1, XM_005264675.2 |
Genome annotation databases
Ensembli | ENST00000409030; ENSP00000386863; ENSG00000163219 [P42331-3] ENST00000409202; ENSP00000386911; ENSG00000163219 [P42331-4] ENST00000409220; ENSP00000386241; ENSG00000163219 [P42331-6] ENST00000467265; ENSP00000420583; ENSG00000163219 [P42331-5] ENST00000497079; ENSP00000417139; ENSG00000163219 [P42331-2] |
GeneIDi | 9938 |
KEGGi | hsa:9938 |
UCSCi | uc002sev.4, human [P42331-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D29642 mRNA Translation: BAA06125.2 Different initiation. AK290396 mRNA Translation: BAF83085.1 AK297056 mRNA Translation: BAH12484.1 AC097495 Genomic DNA No translation available. AC105054 Genomic DNA No translation available. AC112700 Genomic DNA No translation available. CH471053 Genomic DNA Translation: EAW99866.1 BC039591 mRNA Translation: AAH39591.1 |
CCDSi | CCDS33214.2 [P42331-4] CCDS46312.1 [P42331-3] CCDS54363.1 [P42331-5] CCDS54364.1 [P42331-6] |
PIRi | C59430 |
RefSeqi | NP_001007232.2, NM_001007231.2 [P42331-4] NP_001159748.1, NM_001166276.1 [P42331-6] NP_001159749.1, NM_001166277.1 [P42331-5] NP_055697.1, NM_014882.2 [P42331-3] XP_005264732.1, XM_005264675.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1V89 | NMR | - | A | 47-151 | [»] | |
SMRi | P42331 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 115264, 30 interactors |
IntActi | P42331, 25 interactors |
MINTi | P42331 |
STRINGi | 9606.ENSP00000386911 |
PTM databases
CarbonylDBi | P42331 |
iPTMneti | P42331 |
PhosphoSitePlusi | P42331 |
Genetic variation databases
BioMutai | ARHGAP25 |
DMDMi | 238054314 |
Proteomic databases
jPOSTi | P42331 |
MassIVEi | P42331 |
MaxQBi | P42331 |
PaxDbi | P42331 |
PeptideAtlasi | P42331 |
PRIDEi | P42331 |
ProteomicsDBi | 20155 33928 55505 [P42331-1] 55506 [P42331-2] 55507 [P42331-3] 55508 [P42331-4] |
Protocols and materials databases
Antibodypediai | 30948, 389 antibodies |
Genome annotation databases
Ensembli | ENST00000409030; ENSP00000386863; ENSG00000163219 [P42331-3] ENST00000409202; ENSP00000386911; ENSG00000163219 [P42331-4] ENST00000409220; ENSP00000386241; ENSG00000163219 [P42331-6] ENST00000467265; ENSP00000420583; ENSG00000163219 [P42331-5] ENST00000497079; ENSP00000417139; ENSG00000163219 [P42331-2] |
GeneIDi | 9938 |
KEGGi | hsa:9938 |
UCSCi | uc002sev.4, human [P42331-1] |
Organism-specific databases
CTDi | 9938 |
DisGeNETi | 9938 |
GeneCardsi | ARHGAP25 |
HGNCi | HGNC:28951, ARHGAP25 |
HPAi | ENSG00000163219, Group enriched (blood, lymphoid tissue) |
MIMi | 610587, gene |
neXtProti | NX_P42331 |
OpenTargetsi | ENSG00000163219 |
PharmGKBi | PA134941737 |
VEuPathDBi | HostDB:ENSG00000163219.11 |
HUGEi | Search... |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG4270, Eukaryota |
GeneTreei | ENSGT00950000183015 |
HOGENOMi | CLU_020795_1_0_1 |
InParanoidi | P42331 |
OMAi | DVKPQGC |
OrthoDBi | 917149at2759 |
PhylomeDBi | P42331 |
TreeFami | TF323577 |
Enzyme and pathway databases
PathwayCommonsi | P42331 |
Reactomei | R-HSA-194840, Rho GTPase cycle |
Miscellaneous databases
BioGRID-ORCSi | 9938, 1 hit in 872 CRISPR screens |
ChiTaRSi | ARHGAP25, human |
EvolutionaryTracei | P42331 |
GenomeRNAii | 9938 |
Pharosi | P42331, Tbio |
PROi | PR:P42331 |
RNActi | P42331, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000163219, Expressed in blood and 195 other tissues |
ExpressionAtlasi | P42331, baseline and differential |
Genevisiblei | P42331, HS |
Family and domain databases
Gene3Di | 1.10.555.10, 1 hit 2.30.29.30, 1 hit |
InterProi | View protein in InterPro IPR011993, PH-like_dom_sf IPR001849, PH_domain IPR008936, Rho_GTPase_activation_prot IPR000198, RhoGAP_dom |
Pfami | View protein in Pfam PF00169, PH, 1 hit PF00620, RhoGAP, 1 hit |
SMARTi | View protein in SMART SM00233, PH, 1 hit SM00324, RhoGAP, 1 hit |
SUPFAMi | SSF48350, SSF48350, 1 hit |
PROSITEi | View protein in PROSITE PS50003, PH_DOMAIN, 1 hit PS50238, RHOGAP, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RHG25_HUMAN | |
Accessioni | P42331Primary (citable) accession number: P42331 Secondary accession number(s): A8K2Y1 Q8IXQ2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | May 26, 2009 | |
Last modified: | February 10, 2021 | |
This is version 175 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - Human chromosome 2
Human chromosome 2: entries, gene names and cross-references to MIM