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Protein

Rho GTPase-activating protein 25

Gene

ARHGAP25

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state.By similarity

GO - Molecular functioni

GO - Biological processi

  • actin filament organization Source: UniProtKB
  • negative regulation of small GTPase mediated signal transduction Source: UniProtKB
  • phagocytosis, engulfment Source: UniProtKB
  • regulation of small GTPase mediated signal transduction Source: Reactome
  • signal transduction Source: InterPro

Keywordsi

Molecular functionGTPase activation

Enzyme and pathway databases

ReactomeiR-HSA-194840 Rho GTPase cycle

Names & Taxonomyi

Protein namesi
Recommended name:
Rho GTPase-activating protein 25
Alternative name(s):
Rho-type GTPase-activating protein 25
Gene namesi
Name:ARHGAP25
Synonyms:KIAA0053
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

EuPathDBiHostDB:ENSG00000163219.11
HGNCiHGNC:28951 ARHGAP25
MIMi610587 gene
neXtProtiNX_P42331

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000163219
PharmGKBiPA134941737

Polymorphism and mutation databases

BioMutaiARHGAP25
DMDMi238054314

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000567161 – 645Rho GTPase-activating protein 25Add BLAST645

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei362PhosphoserineCombined sources1
Modified residuei395PhosphoserineCombined sources1
Modified residuei406PhosphothreonineBy similarity1
Modified residuei536PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP42331
PaxDbiP42331
PeptideAtlasiP42331
PRIDEiP42331
ProteomicsDBi55505
55506 [P42331-2]
55507 [P42331-3]
55508 [P42331-4]

PTM databases

CarbonylDBiP42331
iPTMnetiP42331
PhosphoSitePlusiP42331

Expressioni

Gene expression databases

BgeeiENSG00000163219
CleanExiHS_ARHGAP25
ExpressionAtlasiP42331 baseline and differential
GenevisibleiP42331 HS

Organism-specific databases

HPAiHPA035346
HPA061395

Interactioni

Protein-protein interaction databases

BioGridi115264, 30 interactors
IntActiP42331, 2 interactors
MINTiP42331
STRINGi9606.ENSP00000386911

Structurei

Secondary structure

1645
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi47 – 56Combined sources10
Beta strandi58 – 61Combined sources4
Beta strandi63 – 71Combined sources9
Beta strandi74 – 82Combined sources9
Beta strandi87 – 90Combined sources4
Beta strandi95 – 100Combined sources6
Beta strandi111 – 117Combined sources7
Beta strandi129 – 132Combined sources4
Helixi136 – 151Combined sources16

3D structure databases

ProteinModelPortaliP42331
SMRiP42331
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP42331

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini46 – 151PHPROSITE-ProRule annotationAdd BLAST106
Domaini159 – 353Rho-GAPPROSITE-ProRule annotationAdd BLAST195

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili541 – 644Sequence analysisAdd BLAST104

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG4270 Eukaryota
ENOG410XRR2 LUCA
GeneTreeiENSGT00760000118863
HOGENOMiHOG000232151
HOVERGENiHBG058875
InParanoidiP42331
KOiK20642
OMAiIFKNEFW
OrthoDBiEOG091G02DD
PhylomeDBiP42331
TreeFamiTF323577

Family and domain databases

Gene3Di1.10.555.10, 1 hit
2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
IPR008936 Rho_GTPase_activation_prot
IPR000198 RhoGAP_dom
PfamiView protein in Pfam
PF00169 PH, 1 hit
PF00620 RhoGAP, 1 hit
SMARTiView protein in SMART
SM00233 PH, 1 hit
SM00324 RhoGAP, 1 hit
SUPFAMiSSF48350 SSF48350, 1 hit
PROSITEiView protein in PROSITE
PS50003 PH_DOMAIN, 1 hit
PS50238 RHOGAP, 1 hit

Sequences (6)i

Sequence statusi: Complete.

This entry describes 6 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P42331-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSLKLPRNWD FNLKVEAAKI ARSRSVMTGE QMAAFHPSST PNPLERPIKM
60 70 80 90 100
GWLKKQRSIV KNWQQRYFVL RAQQLYYYKD EEDTKPQGCM YLPGCTIKEI
110 120 130 140 150
ATNPEEAGKF VFEIIPASWD QNRMGQDSYV LMASSQAEME EWVKFLRRVA
160 170 180 190 200
GTPCGVFGQR LDETVAYEQK FGPHLVPILV EKCAEFILEH GRNEEGIFRL
210 220 230 240 250
PGQDNLVKQL RDAFDAGERP SFDRDTDVHT VASLLKLYLR DLPEPVVPWS
260 270 280 290 300
QYEGFLLCGQ LTNADEAKAQ QELMKQLSIL PRDNYSLLSY ICRFLHEIQL
310 320 330 340 350
NCAVNKMSVD NLATVIGVNL IRSKVEDPAV IMRGTPQIQR VMTMMIRDHE
360 370 380 390 400
VLFPKSKDIP LSPPAQKNDP KKAPVARSSV GWDATEDLRI SRTDSFSSMT
410 420 430 440 450
SDSDTTSPTG QQPSDAFPED SSKVPREKPG DWKMQSRKRT QTLPNRKCFL
460 470 480 490 500
TSAFQGANSS KMEIFKNEFW SPSSEAKAGE GHRRTMSQDL RQLSDSQRTS
510 520 530 540 550
TYDNVPSLPG SPGEEASALS SQACDSKGDT LASPNSETGP GKKNSGEEEI
560 570 580 590 600
DSLQRMVQEL RKEIETQKQM YEEQIKNLEK ENYDVWAKVV RLNEELEKEK
610 620 630 640
KKSAALEISL RNMERSREDV EKRNKALEEE VKEFVKSMKE PKTEA
Note: No experimental confirmation available.
Length:645
Mass (Da):73,435
Last modified:May 26, 2009 - v2
Checksum:i3D54323CA5571754
GO
Isoform 2 (identifier: P42331-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-19: MSLKLPRNWDFNLKVEAAK → MSLGQSACLFLS
     155-155: G → GA
     400-645: TSDSDTTSPT...KSMKEPKTEA → VRCREPSCFH...LSKSSFYWHL

Note: No experimental confirmation available.
Show »
Length:458
Mass (Da):51,779
Checksum:i73BC028BC8A2923E
GO
Isoform 3 (identifier: P42331-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-19: MSLKLPRNWDFNLKVEAAK → MSLGQSACLFLS

Show »
Length:638
Mass (Da):72,431
Checksum:i0B2897FB44880871
GO
Isoform 4 (identifier: P42331-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     155-155: G → GA

Show »
Length:646
Mass (Da):73,506
Checksum:i975E9D31C3740E42
GO
Isoform 5 (identifier: P42331-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     117-155: Missing.

Note: No experimental confirmation available.
Show »
Length:606
Mass (Da):69,017
Checksum:iC203F76F8BA05A6F
GO
Isoform 6 (identifier: P42331-6) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-19: MSLKLPRNWDFNLKVEAAK → MSLGQSACLFLS
     155-155: G → GA

Note: No experimental confirmation available. Gene prediction based on cDNA data.
Show »
Length:639
Mass (Da):72,502
Checksum:iA618E1940495D15D
GO

Sequence cautioni

The sequence BAA06125 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_049142192R → W. Corresponds to variant dbSNP:rs3749130Ensembl.1
Natural variantiVAR_049143555R → S1 PublicationCorresponds to variant dbSNP:rs4241344Ensembl.1
Natural variantiVAR_049144556M → T1 PublicationCorresponds to variant dbSNP:rs10177248Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0373451 – 19MSLKL…VEAAK → MSLGQSACLFLS in isoform 2, isoform 3 and isoform 6. 2 PublicationsAdd BLAST19
Alternative sequenceiVSP_045391117 – 155Missing in isoform 5. 1 PublicationAdd BLAST39
Alternative sequenceiVSP_010275155G → GA in isoform 2, isoform 4 and isoform 6. 2 Publications1
Alternative sequenceiVSP_010276400 – 645TSDSD…PKTEA → VRCREPSCFHWVLPLVQAIP CKACSRVAIWGVLGDAVAVG AAATDSSEHTLKAWPLSKSS FYWHL in isoform 2. 1 PublicationAdd BLAST246

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D29642 mRNA Translation: BAA06125.2 Different initiation.
AK290396 mRNA Translation: BAF83085.1
AK297056 mRNA Translation: BAH12484.1
AC097495 Genomic DNA No translation available.
AC105054 Genomic DNA No translation available.
AC112700 Genomic DNA No translation available.
CH471053 Genomic DNA Translation: EAW99866.1
BC039591 mRNA Translation: AAH39591.1
CCDSiCCDS33214.2 [P42331-4]
CCDS46312.1 [P42331-3]
CCDS54363.1 [P42331-5]
CCDS54364.1 [P42331-6]
PIRiC59430
RefSeqiNP_001007232.2, NM_001007231.2 [P42331-4]
NP_001159748.1, NM_001166276.1 [P42331-6]
NP_001159749.1, NM_001166277.1 [P42331-5]
NP_055697.1, NM_014882.2 [P42331-3]
XP_005264732.1, XM_005264675.2 [P42331-1]
UniGeneiHs.531807

Genome annotation databases

EnsembliENST00000409030; ENSP00000386863; ENSG00000163219 [P42331-3]
ENST00000409202; ENSP00000386911; ENSG00000163219 [P42331-4]
ENST00000409220; ENSP00000386241; ENSG00000163219 [P42331-6]
ENST00000467265; ENSP00000420583; ENSG00000163219 [P42331-5]
ENST00000497079; ENSP00000417139; ENSG00000163219 [P42331-2]
GeneIDi9938
KEGGihsa:9938
UCSCiuc002sev.4 human [P42331-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiRHG25_HUMAN
AccessioniPrimary (citable) accession number: P42331
Secondary accession number(s): A8K2Y1
, B7Z498, E9PFQ7, G5E9G2, Q8IXQ2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: May 26, 2009
Last modified: June 20, 2018
This is version 158 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

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